Objective To evaluate the efficacy and safety of cellular bone allograft(CBA)in ankle arthrodesis.Methods The clinical data of 56 patients with traumatic ankle joint injury and ankle joint injury treated in our hospital from April 2013 to April 2016 were retrospectively analyzed.Imaging was performed at 3 months after surgery,and the visual analogue(VAS)score of the patients,the ankle and foot surgery score(AOFAS),and the SF-36 health status questionnaire score were recorded.The group included 33 patients who were treated with CBA,and patients with simple fusion in the treatment group(n=23).Results 2 months after operation,the fusion rate of CBA group was 87.9% and the fusion rate of without CBA group was 52.2%.3 months after operation,the fusion rate of CBA group was not higher than that of group CBA,and the fusion rate was in group.The data showed a significant improvement in pain,function,and quality of life.No adverse events caused by CBA were observed during the study.Conclusion The rate of fusion was higher in patients treated with bone allograft(CBA)than in patients with autologous bone graft,and the fusion rate was not affected by high risk factors.CBA is a safe and effective graft material for the bone healing of damaged joints,which can provide an effective bone graft substitute for ankle arthrodesis.

Objective To explore the clinical effects of therapeutic methods on child distal fracture of radius.Methods Totally 180 children with distal 212 fractures of radius from January 2012 to December 2015 in some hospital were enrolled into three treatment groups and a control group,of whom,there were 58 ones in a treatment group with external fixation support (external fixation group),58 ones in a treatment group with T-shaped plate internal support (T-shaped plate group),56 ones in a treatment group with Kirschner wire internal fixation (Kirschner wire group) as well as 40 ones in the control group with manual reduction and plaster external fixation.The clinical effects in the four groups were observed,and function scoring was carried out according to Dienst standard.Results The excellent rates in the external fixation group,T-shaped plate group,Kirschner wire group and control group were 72.41％,53.57％,82.76％ and 45％ respectively.The external fixation group had the excellent rate with no significant difference with that in T-shaped plate group (P＞0.05),while obviously higher than that in Kirschner wire group (P＜0.05),and Kirschner wire group had the excellent rate statisticallyhigher than that in the control group (P＜0.05).The external fixation group,T-shaped plate group and Kirschner wire group had the satisfaction significantly higher than that in the control group (P＜0.05).Conclusion Compared with the traditional reduction and Kirschner wire fixation,the external fixation and T-shaped plate fixation have better clinical effects.The treatment of child distal fracture of radius should be planed according to the type of fracture,stability,age,economic condition and substance of the bones of the patient.

Objective To explore the effect of simple active respiratory training method on lung function for cervical spinal cord injury. Methods 24 male patients with cervical spinal cord injury were divided into two groups, the control group (n=14) and the treatment group (n=10). 2 groups all received the same rehabilitation training, while the treatment group was added simple active respiratory training method. Lung function was assessed after eight-week intervention. Results 2 groups improved in percentage of expected value of maximum vital capacity (VCmax), forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), peak expiratory flow (PEF), and maximal voluntary ventilation (MVV) 8 weeks after the treatment(P<0.05), while the treatment group was better than the control group(P<0.05). Conclusion Rehabilitation training can improve lung function of the patients with cervical spinal cord injury, and simple active respiratory training method has better effect.

Acupoint,as the special body surface part where the qi of zang-fu and meridians infuses, arouses the effect of acupuncture and moxibustion. Through the exploration for the origin and the evolution of acupoint, and the integration of the related data about acupuncture-moxibustion theory and modern research, it is believed that acupoint is not fixed and it may be in a state of dynamic change. Also, it is bound up with body function. Acupoint is relatively static and the location is immobile while the body is in normal physiological status. When the body is under pathological condition,however, it is activated and the position is correspondingly changed.
Acupuncture Points ; Acupuncture Therapy ; Humans ; Meridians

Objective To evaluate the clinical factors correlated to eclampsia.MethodsPregnant women with pregnancy－ induced hypertension syndrome (PIH) in 5 years were retrospectiv-ely studied. The relations between eclampsia and prenatal care, PIH degree and the dose of magnesiumsulphate used were evaluated, as well as the eclarnptic aural symptoms and their occurring time. Re-sults The incidence of eclampsia in pregnant women was 0.56 %, and 8.33 % in the PIH cases. Eclamp-sia was significantly correlated to antenatal care. The incidence of eclempsia in cases with moderate andsevere PIH were higher than that with wild PIH. Less than 15g magnesium sulphate (MgSO4) used perday in moderate and severe PIH cases had an eclampsia incidence rate as high as 20.00 %. And the inci-dence in the mild PIH cases was comparatively lower, but still as high as 6.19%. Aural symptoms al-ways appeared prior to eclampsia. Almost all eclampsia cases had the aura within 24h prior to the attack.ConclusionProper and effective antenatal care was of great importance in the prevention of the eclamp-sia. In moderate and severe PIH the eclampsia incidence was higher, but could be decreased by the pro-per use and sufficient amount of MgSO4. It should be emphasized that mild PIH could still develop intoeclampsia. All eclampsia cases had aura which must be paid attention to in PIH cases.

Objective To study the compound oral contraceptives(COC)application in patients with missed abortion after.Methods 600 cases of missed abortion from March 2016～2017 year in March in our hospital,were randomly divided into observation group(n=300)and control group(n=300),the observation group was treated with compound oral contraceptive therapy,the control group were given routine treatment,compared two groups of patients.Results The observation group of patients with vaginal bleeding time,menstrual recovery time was significantly shorter than the control group(P<0.05),endometrial thickness was significantly greater than the control group(P<0.05),the incidence of Intrauterine Residue and uterine cavity adhesion rate,pelvic infection rate was significantly lower than the control group(P<0.05); the two groups of patients with postoperative weight change in March the amount of vaginal bleeding,compared no significant difference,no statistical significance.Conclusion The application of compound oral contraceptives in the treatment of missed abortion patients after curettage,shorten the time of vaginal bleeding,reduce the amount of vaginal bleeding,reduce the incidence of postoperative complications and promote postoperative recovery of menses.

Objective To investigate the effects of biological characters of the human ovarian cell line (OVCAR) by stable transfection short hairpin RNA into the target HMGA1 gene. Methods Experiments were divided into two groups: transfected the OVCAR cells with pSilence4. 1-CMV-Hs plasmid as group A, while transfected OVCAR cells with pSilence4. 1-CMV-Hn plasmid as group B, in which stably transfected cells were gained by antibiotic screening. The comparative expressions of HMGA1 were detected by RT-PCR and western blot. Methyl thiazolyl tetrazolium (MTT) method was applied to measure cell proliferation and at the same time, the cell growth curve was also be mapped. Vitro invasion assay was used to observe the invasion ability of the cancer cells, and the tumor growth of the nude mice inoculated of tumor cells were compared with before and after transfection. Results In group A, the expression level of mRNA and protein HMGA1 gene in OVCAR cells were remarkably reduced before and after the stable transfected with HMGA1 siRNA, in which the percents of mRNA expression were [(86.3±2.7) % vs. (35.8± 3.1) %, P<0.05], the expression of protein were [(68.6±2.8) % vs. (22.3±4.2) %, P<0.05)]. The OVCAR cell growth in stable transfection status was more significantly decreased than that in non-transfection status (P<0.05). In group B, there were no statistical difference in the expression of HMGA1 siRNA, protein and the cell growth between before and after transfection states (P>0.05). The invasion cell numbers were reduced from before to after transfection state in group A [(53±6)vs. (21±6), P< 0.05] ,while there was no significant difference in group B [(51±6)vs. (47±8), P>0.05]. After inoculated transfected cells into nude mice, it took (6.0±0.9) days to grow the planed tumors in group A, which was much shorter than that (12.3±3.9) days in group B (P<0.05 ). After 5 weeks, the tumor weight and volume in group A was were significantly lower than those in group B [(0.8±0.3)g vs. (2.1± 0.4)g, (205±34)mm~3 vs. (987±82) mm~3, all P <0.05]. Conclusion HMGA1 siRNA could remarkably reduce the expression of HMGA1 gene in ovarian cell and also inhabit the ovarian cell growth.

Objective To investigate the relationship between plasma homocysteine levels and lipids in aged patients with acute cerebral infarction(ACI).Methods Fourty-eight cases(observation group),who had been inpatients with cerebral infarction during May 2006 to May 2007,thirty healthy people(control group)contrasted and analyzed.Results The mean plasma homocysteine level and cholesterol(TC),triglycerides(TG),low density lipoprotein(LDL)of aged patients with acute cereral infarction were significantly,higher than those of the control group,but high density lipoprotein(HDL)was lower(P＜0.01).No significant correlation between Hcy level and TC,TG,HDL-C,LDL-C level in serum.Conclusion Plasma homocysteine lexrel and lipids all siginificantly increased in aged patients with acute cereral infarction,and homocysteinemia is not related with lipids,but is an independent risk factor for cerebral infarction.

Objective To investigate the Th1/Th2 cells imbalance in childhood primary nephrotic syndrome(PNS)and its relation to disease activity. Methods Percentage of T-helper-1(Th1) and T-helper-2 (Th2) cells was examined by three-color flow-cytometry in 21 cases of first-onset PNS, 16 cases of remission PNS and 10 healthy children. Results The percentage of Th1, Th2 and Th0 cells in healthy children was (13. 42?4. 36)% , (2. 53 ? 1. 97)% and (1. 25?0. 92)% respectively. In the patients with first-onset PNS, the percentage of Th1, Th2 and Th0 cells was significantly decreased as compared to healthy children [(2. 34 ?2. 09)%, (1.02?0.96)% and (0.40?0.38)% respectively, all P 0. 05]. Because of relatively low percentage of Th1 cells in contrast to Th2 cells in the patients with first-onset PNS, Th1/Th2 ratio was also significantly reduced in those patients, as compared to the patients with remission PNS and the healthy children (2. 43 ? 2.65 vs 4. 17?2. 32 and 5. 41?2. 77, both P