OBJECTIVE:To prepare pazufloxacin mesilate ophthalmic gel and to establish its quality control method.METHODS:The gel was prepared with pazufloxacin mesilate ophthalmic as principal agent and with PVA-124 as base material.The content of pazufloxacin mesilate was determined by HPLC and the stability of the gel was investigated as well.RESULTS:The prepared gel was yellowish liquid and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of pazufloxacin mesilate was 20.0～80.0 ?g?mL-1(r=0.999 9),and the average recovery was 98.67%(RSD=0.79%,n=3).No stratification was noted for the sample within 2-month storage under room temperature.CONCLUSION:The preparation technique of the pazufloxacin mesilate ophthalmic gel is simple and feasible,its quality control method is rapid and the determination result is accurate and reliable.

OBJECTIVE:To prepare penciclovir hollow suppository and establish its quality control method.METHODS:Penciclovir hollow suppository was prepared by heat-melting method.The content of penciclovir was determined by HPLC and the stability of the suppository was investigated as well.RESULTS:The preparation was ivory yellow hollow suppository and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of penciclovir was 5.00～100.00 ?g?mL-1(r=0.999 7),and its average recovery was 98.69%(RSD=0.81%).No obvious change was noted for the property of the suppository within 1 year storage under temperature away from light.CONCLUSION:The preparation technique of the penciclovir hollow suppository is simple and feasible,its quality control method is rapid in operation and its determination result is accurate and reliable.

Objective To explore the curative effects of comprehensive care in the patients with hysteromyoma treated with UAE. Methods120 hysteromyoma patients were divided into 2 groups randomly.The observation group were treated with integrated nurse including psychology,cognition and praxiology.The control group were treated with conventional care. ResultsThe rates of VVR and low-grade fever in the observation group was lower than that of control group(P＜0.05).The spain in the observation group were less than that of control group(P＜0.01). ConclusionThe effective nursing intervention could reduce the complications after operations.

Results of the study showed that Dendrobium candicum and domestically cultivated Panax quinquefolius can alleviate asthenic symptoms in mice with thyroidism-type "Yin" deficiency. Combined use of these two drugs protected these mice from death. Both drug antagonized the inhibitory effect of atropine on salivary secretion in rabbits, and when used in combination even increased salivary secretion in nomal rabbits. These results proved that D. candicum and domestically cultivated P.quinquefolius Possessed the effects of nourishing "Yin" and promoting glandular secretion with a synergistic effect when used in combination.

Objective To determine the optimal time for intravenous injection of bone marrow mesenchymal stem cells(BMSCs)by observing the effects of inflammation after spinal cord injury on the survival and immigration of BMSCs injected intravenously.Methods Forty SD rats were randomized into 1 non-injury group and 7 spinal cord injury(SCI)groups in which the injured spinal cords were harvested at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d respectively.Hematoxylin and eosin(HE)staining was used to e-valuate the histopathologic changes.Myoleperoxidase(MPO)activity was measured to study the duration of polymorphonuclear leukocyte(PMNL)infiltration in the spinal cord after injury.Another 40 SD rats were also randomly divided into 1 non-injury group and 7 transplantation groups in which intravenous injection of BMSCs was performed at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d after SCI.The survival counts and immigration distance of BMSCs in the spinal cord in all the groups were measured.Results The PMNL infiltration into the injured spinal cord and the MPO activity started from 6 h after SCI.There were a large number of infiltrating PMNL in the spinal cord and the MPO activity reached its peak value at 24 h.The infiltrating PMNL in the injured spinal cord began to decrease and the MPO activity began to weaken from 3 d.Proliferation of glial cells and formation of empty holes in the spinal cord started from 5 d.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was longer in the 0 h, 6 h, 12 h, and 24 h transplantation groups.The survival counts of BMSCs were larger and the immigration distance of BMSCs was longer in the 3 d transplantation group.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was shorter in the 5 d and 7 d transplantation groups.Conclusion The optimal time for intravenous injection of BMSCs may be 3 days after SCI.

Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.

ObjectiveLamiophlomis rotate is the rare perfumes Tibetan medicine.This article aimed to explore the current research of Lamiophlomis rotate on the base of literature.MethodsEstablish database of researching literature on Lamiophlomis rotate in domestic published between 2000 and 2010.Results Altogether 210 articles distributed in 112 kinds of journals were found.These articles involved with 8 areas of clinical research(113 article),pharmacological research(18 article),component analysis(35 article),preparation process (15 article),breed and cultivation of seeds (4 article),new drug testing (3 article),biological characteristics and drug economics.32 articles gained fimancial assistance from fund.Three articles on breed and cultivation of seeds gained financial assistance from fund,occupying 75.00％ of such research.Conclusion There were few articles published in core journals,besides relatively scattered distribution of researching institute and journals.Studies on breed and cultivation of seeds should be strengthened.

Objective To explore the short-term efficacy and safety of edaravone combined with clopidogrel in treatment of acute cerebral infarction (ACI).Methods 120 cases of ACI were selected and they were randomly divided into the observation group(60 cases)and the control group according to the visiting order(60 cases).On the basis of routine treatment of ACI,the control group were treated with clopidogrel,and the observation group were added with edaravone combined with clopidogrel.All patients were treated continuously for 14 days.Patients' neural function,activities of daily living,and adverse reactions were evaluated before and after treatment.All patients were followed up for 1 year to record recurrence.Results After treatment,patients' limb movements,neural functions and language had varying degrees of recovery.Total efficiency was 93.33％ and 78.33％ respectively for the observation group and the control group.Grading of the neurologic impairment and life ability of the observation group were both better than the control group.The one-year recurrence rate was 5.77 ％ and 9.26％ for the observation group and the control group,and the difference had statistical significance (P ＜ 0.05).No obvious adverse reaction in the 2 groups was found.Conclusion The curative effect of edaravone combined with clopidogrel is remarkable in treating ACI,and it can remit patients' neurological deficit,improve their daily life ability,reduce the recurrence rate,and has high safety.

Objective To analyze clinical effects of microendoscopic discectomy in the treatment of lumbar disc herniation with or without lumbar spinal stenosis.Methods A total of 216 patients were included in the study.The patient was maintained in the prone position on an arch-like bed.Under C-arm fluoroscopic guidance,a thin pin was inserted down to the facet of the target level.A longitudinal incision was made approximately 1.7 cm at the level of the disease to introduce a series of dilators,over which a working channel was then established.Once extraneous soft tissues were removed,a curette was used to puncture the ligamentum flavum.And the local ligamentum flavum and part of inferior border of the adjacent lamina were removed with a gun-shaped rongeur.The disc space was incised and a discectomy was performed.The lateral recess was cleared of any bone stenosis.Results conversions to open traditional discectomy were conducted in 2 patients because of bleeding.The 216 patients were followed for 3 months ~ 4 years with an average of 2.5 years.According to the Nakai criteria,excellent outcomes were achieved in 210 patients,good in 2 patients,fair in 1,and poor in 3,the rate of excellent or good outcomes being 98.1%(212/216).Of the 3 patients with poor outcomes,2 patients were given a re-operation of traditional surgery 3 months after the microendoscopic discectomy and 1 patient had a missed diagnosis of spinal tuberculosis.Conclusions Microendoscopic discectomy in the treatment of lumbar disc herniation gives little influence to spinal stability and satisfactory clinical effects.

Objective To observe the effects of minimally traumatic puncture drainage of hematoma combined with Salviae Miltiorrhizae injection(丹参注射液) on the edematous volume around the lesion and neural functional defect in patients with hypertensive cerebral hemorrhage.Methods Eighty-nine patients with hypertensive cerebral hemorrhage were randomly divided into two groups: treatment group(n=45) and control group(n=44).All the patients had received minimally traumatic puncture drainage of hematoma,dehydration therapy,hemostatics,anti-inflammatory and anti-hypertensive agents, etc.In addition,Salviae Miltiorrhizae injection was continuously injected intravenously in the patients in the treatment group for three weeks.Cranial CT was performed to measure the volume of edema around the lesion at 7,14 and 21 days before and after operation,neurologic impairment degree scores(NDS) were assessed by National Institutes of Health Stroke Scale(NIHSS),and at the same time the fatality rate and re-hemorrhagic situation were observed at 14 and 30 days before and after initiating the operation.Results The areas of perilesional edema in both groups were conspicuously diminished at 7,14 and 21 days after initiating the operation,the area of perilesional edema diminished gradually,and significantly smaller in the therapeutic group than that in the control group at 14 and 21 days(P