OBJECTIVE:To prepare pazufloxacin mesilate ophthalmic gel and to establish its quality control method.METHODS:The gel was prepared with pazufloxacin mesilate ophthalmic as principal agent and with PVA-124 as base material.The content of pazufloxacin mesilate was determined by HPLC and the stability of the gel was investigated as well.RESULTS:The prepared gel was yellowish liquid and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of pazufloxacin mesilate was 20.0～80.0 ?g?mL-1(r=0.999 9),and the average recovery was 98.67%(RSD=0.79%,n=3).No stratification was noted for the sample within 2-month storage under room temperature.CONCLUSION:The preparation technique of the pazufloxacin mesilate ophthalmic gel is simple and feasible,its quality control method is rapid and the determination result is accurate and reliable.

OBJECTIVE:To prepare penciclovir hollow suppository and establish its quality control method.METHODS:Penciclovir hollow suppository was prepared by heat-melting method.The content of penciclovir was determined by HPLC and the stability of the suppository was investigated as well.RESULTS:The preparation was ivory yellow hollow suppository and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of penciclovir was 5.00～100.00 ?g?mL-1(r=0.999 7),and its average recovery was 98.69%(RSD=0.81%).No obvious change was noted for the property of the suppository within 1 year storage under temperature away from light.CONCLUSION:The preparation technique of the penciclovir hollow suppository is simple and feasible,its quality control method is rapid in operation and its determination result is accurate and reliable.

Objective To explore the curative effects of comprehensive care in the patients with hysteromyoma treated with UAE. Methods120 hysteromyoma patients were divided into 2 groups randomly.The observation group were treated with integrated nurse including psychology,cognition and praxiology.The control group were treated with conventional care. ResultsThe rates of VVR and low-grade fever in the observation group was lower than that of control group(P＜0.05).The spain in the observation group were less than that of control group(P＜0.01). ConclusionThe effective nursing intervention could reduce the complications after operations.

Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.

To assure the quality and safety of nuclear medicine imaging,quality control for the imaging equipment is essential.Quality control consists of a series of special tests for assurance and adjustment of equipment accuracy according to specific standards.The development and status of quality control and the standards for nuclear medicine imaging equipment are investigated and reviewed in this study.The results show that the quality control and feasibility of the standards are both in need of improvement in China.

Objective To observe the effects of minimally traumatic puncture drainage of hematoma combined with Salviae Miltiorrhizae injection(丹参注射液) on the edematous volume around the lesion and neural functional defect in patients with hypertensive cerebral hemorrhage.Methods Eighty-nine patients with hypertensive cerebral hemorrhage were randomly divided into two groups: treatment group(n=45) and control group(n=44).All the patients had received minimally traumatic puncture drainage of hematoma,dehydration therapy,hemostatics,anti-inflammatory and anti-hypertensive agents, etc.In addition,Salviae Miltiorrhizae injection was continuously injected intravenously in the patients in the treatment group for three weeks.Cranial CT was performed to measure the volume of edema around the lesion at 7,14 and 21 days before and after operation,neurologic impairment degree scores(NDS) were assessed by National Institutes of Health Stroke Scale(NIHSS),and at the same time the fatality rate and re-hemorrhagic situation were observed at 14 and 30 days before and after initiating the operation.Results The areas of perilesional edema in both groups were conspicuously diminished at 7,14 and 21 days after initiating the operation,the area of perilesional edema diminished gradually,and significantly smaller in the therapeutic group than that in the control group at 14 and 21 days(P

Objective To determine the optimal time for intravenous injection of bone marrow mesenchymal stem cells(BMSCs)by observing the effects of inflammation after spinal cord injury on the survival and immigration of BMSCs injected intravenously.Methods Forty SD rats were randomized into 1 non-injury group and 7 spinal cord injury(SCI)groups in which the injured spinal cords were harvested at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d respectively.Hematoxylin and eosin(HE)staining was used to e-valuate the histopathologic changes.Myoleperoxidase(MPO)activity was measured to study the duration of polymorphonuclear leukocyte(PMNL)infiltration in the spinal cord after injury.Another 40 SD rats were also randomly divided into 1 non-injury group and 7 transplantation groups in which intravenous injection of BMSCs was performed at 0 h, 6 h, 12 h, 24 h, 3d, 5 d, and 7 d after SCI.The survival counts and immigration distance of BMSCs in the spinal cord in all the groups were measured.Results The PMNL infiltration into the injured spinal cord and the MPO activity started from 6 h after SCI.There were a large number of infiltrating PMNL in the spinal cord and the MPO activity reached its peak value at 24 h.The infiltrating PMNL in the injured spinal cord began to decrease and the MPO activity began to weaken from 3 d.Proliferation of glial cells and formation of empty holes in the spinal cord started from 5 d.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was longer in the 0 h, 6 h, 12 h, and 24 h transplantation groups.The survival counts of BMSCs were larger and the immigration distance of BMSCs was longer in the 3 d transplantation group.The survival counts of BMSCs were smaller but the immigration distance of BMSCs was shorter in the 5 d and 7 d transplantation groups.Conclusion The optimal time for intravenous injection of BMSCs may be 3 days after SCI.

BACKGROUND:The research for mesenchymal stem cels in the treatment of liver cirrhosis has made great progress. However,t-test analysis is often misused in clinical practice. OBJECTIVE:To analyze the efficacy and safety of umbilical cord mesenchymal stem cel therapy for liver cirrhosis by repeated measurement method. METHODS: A total of 27 patients with decompensated liver cirrhosis underwent conventional medical treatments, including liver protection treatment and symptomatic treatment. At 1 week after hospitalization, patients were given intravenous transplantation of umbilical cord mesenchymal stem cels (passages 2-4), cel viability≥ 90%, stem cel counting≥ 2×107 cels, four times with an interval of 5-7 days. Analysis of variance based on repeated measurement data was performed to analyze the liver function changes at different time after umbilical cord mesenchymal stem cel transplantation. RESULTS AND CONCLUSION: The results of univariate repeated measurement showed that at 2 and 3 months after treatment, the serum albumin level was increased and the total bilirubin level in serum was decreased significantly (P < 0.05); at 3 months after treatment, the content of aspartate aminotransferase decreased, and the content of cholinesterase increased significantly (P < 0.05). No patient appeared to have liver and other organ tumors during the observation period. These findings indicate that umbilical cord mesenchymal stem cel transplantation is effective and safe in the treatment of liver cirrhosis, which can significantly improve patient's liver functions.

Objective To investigate the effects of different selective attention on the amplitudes of distortion product otoacoustic emission (DPOAE) .Methods DPOAE measurements were performed in 30 young adults (60 ears) in no task (baseline) ,visual selective attention and auditory selective attention ,respectively .The suppression of DPOAE amplitudes were observed in different selective attentions .The visual selective attention was to counter the number of letter Q presented on computer screen .The auditory selective attention was to counter the number of 2 .0 kHz toneburst in the insert earphone .Results Visual and auditory selective attention both decreased the DPOAE amplitudes in mid - low frequencies (0 .75 ~ 2 .0 kHz) .The suppression effects of visual selective attention were significant stronger than that of auditory attention in 0 .75 ~ 1 .0 kHz .The suppression amplitudes were 8 .54 ± 4 .76 and 5 .27 ± 2 .32 dB at 0 .75 kHz ,respectively .They were 7 .66 ± 5 .22 and 3 .22 ± 2 .15 dB at 1 .0 kHz ,re‐spectively .There were significant differences between the two selective attentions suppression (P< 0 .05) .Conclu‐sion Visual and auditory selective attention can both decrease the DPOAE amplitudes .The suppression effects of visual selective attention were significant stronger than that of auditory attention .

Results of the study showed that Dendrobium candicum and domestically cultivated Panax quinquefolius can alleviate asthenic symptoms in mice with thyroidism-type "Yin" deficiency. Combined use of these two drugs protected these mice from death. Both drug antagonized the inhibitory effect of atropine on salivary secretion in rabbits, and when used in combination even increased salivary secretion in nomal rabbits. These results proved that D. candicum and domestically cultivated P.quinquefolius Possessed the effects of nourishing "Yin" and promoting glandular secretion with a synergistic effect when used in combination.