Objective To study the preventive effect of high-dose immuno-chemotherapy on the recurrence and metastasis of renal cell carcinoma(RCC). Methods Overall 18 patients with RCC(13 men and 5 women;mean age,56 years,range,37 to 78 years) underwent radical nephrectomy.These cases were assigned to 2 groups.①Immuno-chemotherapy group included 13 cases (10 men and 3 women),whose tumor stages were T 1 (1 case),T 2 (8) and T 3(4).They were treated with high-dose subcutaneous interleukin-2(IL-2) in combination with interferon-?(IFN-?) and venous infusion 5-fluorouracil(5-Fu) 10 days after operation.②Control group included 5 cases (3 men and 2 women),whose tumor stages were T 1(1 case),T 2(3),T 3(1).They didn’t receive immuno-chemotherapy.The immuno-chemotherapeutic courses consisted of IL-2 (8?10 6 U,3 times/w,weeks 1 and 4;4?10 6 U,3 times/w,weeks 2 and 3);IFN-? 6?10 6 U,3 times/w,weeks 2,3,6 and 7;9?10 6 U,3 times/w,weeks 5 and 8);and 5-Fu (1000 to 1250 mg,once a week,weeks 5 to 8).Such therapeutic cycle could be repeated at 3-month intervals.Follow-up was conducted with hemogram,hepatic and renal function,ultrasonography of liver and kidney,chest X-ray every month after treatment.Long-term outcomes for these patients were analyzed. Results Follow-up of 60 to 87 months was carried out after 1 to 4 therapeutic cycles(mean,1.9 cycles) in the 13 cases of immuno-chemotherapy.Of them,12 cases experienced cancer-free survival.Only 1 case had metastasis in thigh bone 43 months after operation.No severe side effects occurred.5 cases in control group were found with metastasis in 3 to 14 months after operation.The mean survival of control group was 12.8 months (range,6 to 21 months). Conclusions Immuno-chemotherapy with high-dose subcutaneous IL-2 in combination with IFN-? and venous infusion of 5-Fu is safe and effective in prevention of the recurrence and metastasis of RCC.

Objective To evaluate objectively the traditional methods on the assessment of bladder outlet obstruction(BOO) due to BPH. Methods Correlation between the urodynamic findings and the traditional diagnostic parameters such as age,IPSS,Vp,Qmax z and PVRr was studied.The clinical prostatic score(CPS),derived from multiple regression of clinical parameters depending on URA,was evaluated. Results The parameters such as age,IPSS,Vp,Qmax z and PVRr were evaluated with reference to pressure flow study.Every parameter alone was not enough for BOO diagnosis. The regression equation was CPS=49.8-3.3 Qmax z+0.5 IPSS+0.2 Vp+7.5 PVRr.Correlation coefficient between CPS and urodynamic findings was 0.629 and was significantly higher than that of any clinical parameter alone.With CPS≥35,the sensitivity was 83.7% and specificity 85.8% for the diagnosis of BOO.With CPS

[Objective]To investigate the causes and treatment methods of postoperative complications of femoral fractures treated with interlocking intramedullary nails.[Method]A total of 243 cases of femoral fractures from Jan 1999 to Dec 2004 were treated with interlocking intramedullary nails.There were 14 cases having complications,delayed union in 1,nonunion in 3,nailing failure in 6(locking scerw loosen in 4,locking scerw folding in 1,locking scerw breakage in 2),nail breakage in 1,re-fracture in 1 and knee ankylosis in 1.Two cases with delayed union to convert the fixation from statictody namicin time;3 cases with nonunion were treated with bone grafting.Breakage nail and re-fractuer cases were treated with enlarging intramedullary tunels,exchanging nails and bone grafting.One of locking scerw loosen were treated with exchanging nails and bone grafting.Knee ankylosis were treated with quadriceps plasty.[Result]Through treatment,the delayed union and the nonunion were all healed and got recovery of knee function.[Conclusion]Though it has certain complications,the interlocking intramedullary nails used for femoral fractures have more advantages than other internal fixations.It needs to take valid measures to reach a satisfying result when complication appears.

Objective To evaluate the effects of nutritional intervention on the incidence of malnutrition in patients on maintenance hemodialysis (MHD).Methods Totally 196 patients on MHD were randomly assigned to nutritional intervention group (n =98) and control group (n =98).Nutritional intervention or free diet were given in these two groups.The incidence of malnutrition in these two groups was recoded.Results The baseline age,biochemical indicators,and incidence of malnutrition were matched in these two groups (P ＞0.05).After 6 months,although the number of the patients with malnutrition [35.7％ (35/98)] was slightly increased in the intervention group,it showed no significant difference when compared with the baseline level [33.7％ (33/98),P ＞0.05].In the control group,however,the incidence of malnutrition [59.2％ (58/98)] significantly increased (P ＜0.05).The incidence of malnutrition was significantly lower in the intervention group than in the control group (P ＜ 0.05).Conclusion Nutritional intervention can lower the incidence of malnutrition in patients on MHD.

0.05),but 5-Aza at the concentration of 15?mol/L inhibited the growth of MSCs(P

Objective To improve the success rate of insertion of gastric lavage canal in patientswith tracheal intubation. Methods 70 patients with organophosphoms pesticide intoxication who neededgastric lavage after tracheal intubation were randomized into the test group and the control group, 35 easesin each. In test group, lavage canal was inserted through bite block orally and the bedside was raised till 70to 80 in angle. While in the control group, lavage canal was inserted directly through mouth with the posi-tion of hypsokinesis. The success rate of intubation was compared between the two groups. Results One-time success rate was 94.2% in the test group,which was statistically different from that of the control group(37.1%), P < 0.01. The complication of intubation in the test group was also lower than that of the controlgroup,which had statistical difference. Conclusions Through improvement of position to raise the bedsidetill 70 to 80 in angle, the insertion of gastric lavage canal through bite block orally was much better than thenormal one and it is worth applying.

Objective Through the 2003 -2012 epidemic encephalitis B cases analysis ,to study the prevalence of epidemic en-cephalitis B in Shaoguan ,and to provide the scientific basis for the prevention and control epidemic encephalitis B .Methods Active surveillance of suspected cases ,retrospective analysis of laboratory confirmed cases related materials were done .Results There were 47 cases who suffered from epidemic encephalitis B in Shaoguan from 2003 to 2012 ,and 2 death cases occurred .Epidemic en-cephalitis B incidence in years was (0 -0 .531) /10 million .There was a morbidity peak in June and July ,which accounted for 87 .23% of the total incidence ,20 cases occurred in June(42 .55% )and 21 cases occurred in July(44 .68% ) .Men suspected cases in 130 cases ,31 laboratory confirmed cases .Women suspected cases in 86 cases ,16 laboratory confirmed cases .The positive cases of male was more than female .In 47 epidemic encephalitis B cases ,0- <4 years old 6 cases ,4- <7 years old 22 cases ,7- <20 years old 17 cases ,≥ 20 years old 2 cases .Most of the patients were preschool children and students ,accounting for 82 .97% .There were 32 cases with no or uncertain history of vaccination ,accounting for 68 .08% of the total cases .15 cases had history of immunity ,ac-counting for 31 .92% of the total cases .Conclusion Prevention of epidemic encephalitis B should pay more attention to epidemic en-cephalitis B vaccination .Mosquito control work of rural and peri-urban areas should be strengthened .

Objective To study surgical treatment of complex fractures of the tibial plateau. Methods Forty eight cases of complex fractures of the tibial plateau were treated with internal fixation from January 1997 to December 2001. According to Schatzker classification, 12 cases were type Ⅲ, 9 cases type Ⅳ, 14 cases type Ⅴand 13 cases type Ⅵ. They were treated with open reduction and internal fixation with buttress plates and allograft implantation for defective fractures. Results Forty six cases of the forty eight were followed up for 1 to 4 years. The excellent and good rate was 77%. Conclusion The complex fractures of the tibia plateau should be treated surgically, and the operation procedures should be varied according to the fracture types.

Objective To investigate the changes of urinary bladder structure and function of aging rats. Methods The aging group and the young adult group were composed of 24 month old rats and 6 month old rats, respectively. The urodynamic changes were studied with filling cystometry, structural changes by Masson stain and image analysis. Results Filling cystometry revealed that the maximum bladder capacity increased in the aging rats. No difference in leak point pressure was found in the two groups. The occurrence rate of the detrusor instability in aging group was higher than that in young adult group. In bladder structure, fibrous tissue increased significantly but smooth muscle decreased significantly in the detrusor of aging rats. Conclusion Aging may lead to changes of rat bladder structure and function, but its mechanism needs to be further studied.

Objective To establish and optimize three-cube FRET assay in living cells and analyze subunit assembling of iGluR receptors. Methods Taking HEK293 cells cotransfed with pECFP and pEYFP as negative control, and those transfected with pECFP-YFP as positive control,different calculation methods using fluorescence microscopy were compared. Results These calculation methods were all suitable for FRET measurement in the system. but the measurement results were affected by the ratio of Donor/Acceptor (D/A) in some degree,and different calculation methods have different optimized conditions. FRET measurement using FR value showed subunit specific assembly of iGluR subtypes.Conclusion There are different optimized conditions for these different calculation methods in the three-cube FRET measurement system,and a further evidence is provided for subunit specific assembling of iGluR subtypes from the FRET assay.