Purpose To investigate the curative effect of electroacupuncture on anxiety neurosis. Method Twenty-six female patients with extensive anxiety neurosis were selected according to CCMD-2-R diagnostic criteria. Evaluations based on STAI were made before and after electroacupuncture treatment for 4 times. Points Baihui ( GV 20), Shangxing ( GV 23), Neiguan ( PC 6),Shenmen ( HT 7 ), Zusanli ( ST 36), Sanyinjiao ( SP 6) and Taichong (LR 3 ) were selected for electroacupuncture treatment. One course of treatment consisted of 10 days and a total of 3 courses were carried out. Results The effective rate was 80.8% at the completion of treatment. The scores of state-anxiety index (S-Al) and typical-anxiety index (T-Al) were significantly decreased as compared with before the treatment ( P < 0.01 ), especially in the patients with recovery or improvement (P <0.01 and P < 0. 05 ). Conclusion Electroacupuncture treatment has a good effect on extensive anxiety disorder and can avoid the dependence of the patients on anxio lytics.

Objective To observe the effect of comprehensive rehabilitation on the motor function and activity of daily living in patients with acute cerebral infarction. Methods 110 patients with acute cerebral infarction were randomly divided into rehabilitation group and control group. The patients in control group were treated with medicine, while the patients in rehabilitation group were treated with comprehensive therapy, including medicine, physical therapy and neuromuscular electrical stimulation. They were assessed with the simplified Fugl-Meyer assessment (FMA), modified Barthel index (MBI), and the clinical defect of neurol function before and 4 weeks after treatment. Results The scores of FMA and MBI improved significantly in both groups after treatment, and improved more in rehabilitation group than in control group (P<0.05). The effective rate was 75% in the rehabilitation group, and 56% in the control group (P<0.05). Conclusion Comprehensive rehabilitation can promote the recovery of motor function and activity of daily living in patients with acute cerebral infarction.

Objective To observe the effects of early rehabilitation training on serum brain-derived neurotrophic factor (BDNF) expressionand motor function in patients with acute cerebral infarction. Methods 48 patients with acute cerebral infarction were randomly dividedinto rehabilitation group (n=24) and control group (n=24). The control group accepted routine medication, while the rehabilitation group acceptedearly rehabilitation training in addition. They were assessed with Fugl-Meyer Assessment (FMA), and the expression of BDNF in serumwas detected before and after treatment. Results The expression of BDNF and the score of FMA increased significantly after treatmentin both groups (P<0.05), but increased more in the rehabilitation group than in the control group (P<0.05). Conclusion Early rehabilitationtraining can promote the expression of serum BDNF and recovery of motor function in patients with acute cerebral infarction.

Objective To explore the feasibility of 256-slice whole-brain CT perfusion (CTP) in evaluate graft reperfusion after surgical revascularization and hemodynamic alterations before and after surgery in Moyamoya disease. Methods Twenty-five cases with Moyamoya disease were scanned on a 256-slice CT.CTP was performed pre- and post- surgical revascularization. The wolumetric CT angiographic ( CTA ) images were generated from volumetric data acquired at the arterial phase of CTP. CBF, CBV, TTP and MTT were measured in functional maps at the operated side within middle cerebral artery perfusion areas and contralateral mirroring areas. Relative CBF( rCBF), relative CBV (rCBV), relative TTP (rTTP), relative MTT (rMTT) were also obtained. Differences in perfusion CT values pre- and post operation were assessed with the paired t test or matched-pairs signed-ranks test. Data with normal distribution was present as : (x-)± s,while those with the non-normal distribution were present as M ( P25-P75 ). Results All the direct graft patencies were displayed on volumetric CTA. No significant differences were found between volumetric CTA and conventional CTA. Postoperative CBF, rCBF and rCBV values of the operated side [ 72. 86 (55.54-112. 19) ml · 100 g-1 · min-1 , 1. 31 ( 1.05-1.73), 1.45 ±0. 62] were significantly higher than those before operation [46.72(28.57-57.67) ml · 100 g-1 · min-1, 0.53(0.33-0.82), 1.01 ±0.36](Z=- 2.72, - 2. 98, t = - 2. 85, P ＜ 0. 05 ). Postoperative MTT, TTP and rTTP values of the operated side [ (3.98 ± 2. 36 ) s, ( 17.56 ± 4. 38 ) s, 1.01 ± 0. 09 ] were significantly lower than those before operation [(5.43±2.07) s,(19.40±3.87) s,1.14±0.28] (t=2.41,2.17,2.17, respectively, P＜0.05).However, no significant differences were detected for changes of CBV and rMTT after revascularization ( P ＞0. 05). Conclusion 256-slice CT has the potential value for the non-invasive assessment of both the graft patency and cerebral hemodynamics changes in moyamoya disease after surgery with administration of one contrast medium bolus in a single examination.

Objective To study the clinical,electrophysiological,athological and genomic features of hereditary neuropathy with liability to pressure palsies (HNPP) and increase the understanding and diagnostic level of this disease.Methods Eleven patients with HNPP,met Gouider diagnostic criteria and admitted to our hospital from March 1999 to December 2014,accepted detailed clinical examinations,electromyogram,sural nerve biopsies.Multiplex ligation-dependent probe amplification was used to detect peripheral myelinprotein 22(PMP22) gene deletion on chromosome 17P11.2.Results Eight patients came from two families and it was consistent with automsomal dominant inheritance.Age at onset was on teen-agers.Clinical manifestations were characterized by recurrent mononeruopathies.Symptoms often disappeared spontaneously after a few days or a few months.Nerve conduction studies showed a sensori-motor demyelinating polyneuropathy with conduction abnormalities preferentially localized at common entrapment sites.The nerve biopsy showed the presence of some large thickened myelinated fibers,but the axons were normal.Gene mutation analysis showed that two patients had large fragment tandem deletions containing PMP22.Conclusions Although HNPP is concerned with heredity,there are also some sporadic cases to be found.Electrophysiologic examination is an important screen method.The definitive diagnosis is dependent on PMP22 mutation detection.

Objective To explore the effect of repeated hypoxic preconditioning (RHP) on renal ischemia-reperfusion-induced hepatic dysfunction in rats and the underlying mechanism. Methods A total of 120 normal SD rats were randomly divided into 4 groups (n=40), namely RHP surgical group, RHP sham-operated (RHPS) group, nonhypoxic surgical group (IRI group), and nonhypoxic sham-operated group (S group). The rats in the hypoxic groups were exposed to hypoxia in a hypoxic chamber for 5 days prior to establishment of renal ischemia-reperfusion model by resection of the right kidney and clamping the left renal hilum. Serum alanine aminotransferase (ALT), IL-17A, TNF-α, liver superoxide dismutase (SOD) and nitric oxide (NO) were detected at 2, 8 and 24h after reperfusion, and Western blotting was used to determine the expression of p-PI3K and p-AKT;HE staining was used to observe the structural changes in the liver. Results Compared with IRI group, RHP group showed significantly milder hepatic damage, lower ALT levels and higher NO levels at 2, 8, and 24 after reperfusion (P<0.05);TNF-αlevels were lowered at 24 h (P<0.05) and SOD increased at 8 h after the reperfusion (P<0.05). Compared with S group, IRI group and RHP group showed significantly higher IL-17A levels (P<0.05) but without significant difference between the latter two groups (P>0.05). The expressions of p-PI3K and P-Akt in RHP group were significantly higher than those in IRI group (P<0.05), especially at 8 h after reperfusion (P<0.05). Conclusion Repeated hypoxic preconditioning can attenuate hepatic injury induced by renal ischemia-reperfusion injury in rats.

OBJECTIVES: In light of the rise in the global aging population, this study investigated the potential of the oxidative balance score (OBS) as an indicator of phenotypic age acceleration (PhenoAgeAccel) to better understand and potentially slow down aging. METHODS: Utilizing data from the National Health and Nutrition Examination Survey collected between 2001 and 2010, including 13,142 United States adults (48.7% female and 51.2% male) aged 20 and above, OBS and PhenoAgeAccel were calculated. Weighted generalized linear regression models were employed to explore the associations between OBS and PhenoAgeAccel, including a sex-specific analysis. RESULTS: The OBS demonstrated significant variability across various demographic and health-related factors. There was a clear negative correlation observed between the higher OBS quartiles and PhenoAgeAccel, which presented sex-specific results: the negative association between OBS and PhenoAgeAccel was more pronounced in male than in female. An analysis using restricted cubic splines revealed no significant non-linear relationships. Interaction effects were noted solely in the context of sex and hyperlipidemia. CONCLUSIONS A higher OBS was significantly associated with a slower aging process, as measured by lower PhenoAgeAccel. These findings underscore the importance of OBS as a biomarker in the study of aging and point to sex and hyperlipidemia as variables that may affect this association. Additional research is required to confirm these results and to investigate the biological underpinnings of this relationship.

Objective To explore the effect of repeated hypoxic preconditioning (RHP) on renal ischemia-reperfusion-induced hepatic dysfunction in rats and the underlying mechanism. Methods A total of 120 normal SD rats were randomly divided into 4 groups (n=40), namely RHP surgical group, RHP sham-operated (RHPS) group, nonhypoxic surgical group (IRI group), and nonhypoxic sham-operated group (S group). The rats in the hypoxic groups were exposed to hypoxia in a hypoxic chamber for 5 days prior to establishment of renal ischemia-reperfusion model by resection of the right kidney and clamping the left renal hilum. Serum alanine aminotransferase (ALT), IL-17A, TNF-α, liver superoxide dismutase (SOD) and nitric oxide (NO) were detected at 2, 8 and 24h after reperfusion, and Western blotting was used to determine the expression of p-PI3K and p-AKT;HE staining was used to observe the structural changes in the liver. Results Compared with IRI group, RHP group showed significantly milder hepatic damage, lower ALT levels and higher NO levels at 2, 8, and 24 after reperfusion (P<0.05);TNF-αlevels were lowered at 24 h (P<0.05) and SOD increased at 8 h after the reperfusion (P<0.05). Compared with S group, IRI group and RHP group showed significantly higher IL-17A levels (P<0.05) but without significant difference between the latter two groups (P>0.05). The expressions of p-PI3K and P-Akt in RHP group were significantly higher than those in IRI group (P<0.05), especially at 8 h after reperfusion (P<0.05). Conclusion Repeated hypoxic preconditioning can attenuate hepatic injury induced by renal ischemia-reperfusion injury in rats.

Pelvic floor muscle training (PFMT) is the first-line intervention for non-surgical treatment of female patients with stress urinary incontinence (SUI). However, due to the particularity of the physiological position of the pelvic floor muscles, 13% to 53% of patients are unable to perform PFMT correctly after the initial visit. In view of this, scholars at China and abroad put forward some other sports rehabilitation methods and achieve good clinical results. In this paper, exercise rehabilitation methods for female SUI patients were reviewed, including PFMT, core muscle training, Pilates, yoga, physical activities, etc., in order to provide reference for medical personnel to formulate scientific and feasible exercise rehabilitation programs for female SUI patients.

Background::Given that seizures may be triggered by vaccination, this study aimed to evaluate the risk and correlative factors of seizures in patients with epilepsy (PWE) after being vaccinated against coronavirus disease 2019 (COVID-19).Methods::This study retrospectively enrolled PWE who were vaccinated against COVID-19 in the epilepsy centers of 11 hospitals in China. We divided the PWE into two groups as follows: (1) patients who developed seizures within 14 days of vaccination were assigned to the SAV (with seizures after vaccination) group; (2) patients who were seizure-free within 14 days of vaccination were assigned to the SFAV (seizure-free after vaccination) group. To identify potential risk factors for seizure reccurence, the binary logistic regression analysis was performed. Besides, 67 PWE who had not been vaccinated were also included for elucidating the effects of vaccination on seizures recurrence, and binary logistic regression analysis was performed to determine whether vaccination would affect the recurrence rate of PWE who had drug reduction or withdrawal.Results::The study included a total of 407 patients; of which, 48 (11.8%) developed seizures within 14 days after vaccination (SAV group), whereas 359 (88.2%) remained seizure-free (SFAV group). The binary logistic regression analysis revealed that duration of seizure freedom ( P < 0.001) and withdrawal from anti-seizure medications (ASMs) or reduction in their dosage during the peri-vaccination period were significantly associated with the recurrence of seizures (odds ratio= 7.384, 95% confidence interval = 1.732–31.488, P = 0.007). In addition, 32 of 33 patients (97.0%) who were seizure-free for more than three months before vaccination and had a normal electroencephalogram before vaccination did not have any seizures within 14 days of vaccination. A total of 92 (22.6%) patients experienced non-epileptic adverse reactions after vaccination. Binary logistic regression analysis results showed that vaccine did not significantly affect the recurrence rate of PWE who had the behavior of ASMs dose reduction or withdrawal ( P = 0.143). Conclusions::PWE need protection from the COVID-19 vaccine. PWE who are seizure-free for >3 months before vaccination should be vaccinated. Whether the remaining PWE should be vaccinated depends on the local prevalence of COVID-19. Finally, PWE should avoid discontinuing ASMs or reducing their dosage during the peri-vaccination period.