Objective To detect the expression of chemoattractant receptors, CXCR4 and formyl peptide receptor (FPR), in rat glioma cell line C6 and to explore their relation to tumor differentiation and angiogenesis. Methods The expression of CXCR4 and FPR by C6 cells were detected with immunocytochemistry. The differentiatial degrees were also detected to analyze the relationship of chemoattractant receptors with angiogenesis and malignancy of glioma. Results C6 cells were of a long spindle shape, expressing high level of vimentin but relatively low level of glial fibrillary acidic protein (GFAP). C6 cells were positive for CXCR4 and FPR in the cytoplasm and strongly positive for vascular endothelial growth factor (VEGF). Conclusion C6 cell line is poorly differentiated and highly expresses chemoattractant receptors CXCR4 and FPR, both of which might be related to the angiogenic potentials.

Objective:To explore the clinical efficacy of Paishi granules on promoting stone expulsion after extracorporeal shock wave lithotripsy ( ESWL) . Methods:A retrospective study method was adopted. Totally 510 cases of lithangiuria patients were divided into the treatment group and the control group with 255 cases in each. The control group was treated by ESWL, and the treatment group was treated by Paishi granules after ESWL. The stone expulsion rate,clinical efficacy and adverse reactions in the two groups were stud-ied and compared. Results:The stone expulsion rate of the observation group was 78. 8%,which was significantly higher than that of the control group (51. 0%, P<0. 05). The curative rate of the observation group was 72. 9% with the total effective rate of 92. 5%, that of the control group was 43. 9% with the total efficiency of 82. 4%,and the differences between the two groups were statistically significant (P<0. 05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0. 05). Conclusion:Using Paishi granules after ESWL can promote stone expulsion with high efficacy and slight adverse reactions.

BACKGROUND:A high incidence of deep vein thrombosis after total hip replacement does great harm to patients,so it is necessary to look for a safe and effective prevention program after total hip replacement to reduce the harm of deep vein thrombosis.OBJECTIVE:To observe the influence of comprehensive prevention scheme on deep vein thrombosis after total hip replacement.METHODS:102 cases undergoing total hip replacement were randomly divided into observation group and control group with 51 cases in each group.Patients in the observation group received the comprehensive prevention program,which is composed of three interventions: sequential cross combined with low molecular heparin,intermittent pneumatic pressure therapy and continuous femoral nerve block analgesia.In the control group,patients received low-molecular-weight heparin for anticoagulation therapy by the doctor according to his clinical experience,or intermittent pneumatic pressure therapy.The occurrence and distribution of deep vein thrombosis were compared in patients of both groups.At the same time,platelet and hemoglobin were recorded.RESULTS AND CONCLUSION:In the observation group,three patients suffered from deep vein thrombosis with an incidence of 6％.In the control group,11 patients affected deep vein thrombosis,with an incidence of 22％.There were significant differences between the two groups (P＜ 0.05).No significant difference in constituent ratio of the distribution of deep vein thrombosis was detected in the distal vein and proximal vein in patients of both groups (P＞ 0.05).Hemoglobin value was lower at 1 day after surgery compared with that pre-operation in the two groups (P＜0.05).Platelet value was lower at 1 day after surgery compared with that pre-operation in the two groups (P＞0.05).No significant difference in hemoglobin and platelet values was detected between two groups at 1 and 6 days after surgery (P＞ 0.05).Results indicated that the comprehensive prevention program containing the sequential and cross combined with low molecular heparin,intermittent pneumatic pressure therapy and continuous femoral nerve block analgesia can significantly reduce the incidence of deep vein thrombosis,and no serious complications occurred,and the effects are affirmative and reliable.

Objective To investigate the coagulation function,the related factors and prognosis of preterm infants with periventricular hemorrhage-intraventricular hemorrhage (PVH-IVH).Methods Eighty-one preterm infants with PVH-IVH and 81 infants with non-PVH-IVH as a control were enrolled in the study.The changes in activated thromboplastin time(PT),activated partial thromboplastin time (APTT),Fibrinogen and D-dimer were determined by enzyme-linked immunosorbent assay.Clinical factors which were related to PVH-IVH in infants were analyzed with statistical analysis and prognosis was assessed with neonatal behavioral neurological assessment (NBNA).Results The average of gestational age in PVH-IVH group was(30.9 ± 1.79)weeks,in control group were (31.36 ± 1.39)weeks,and there was significant difference between the 2 groups (F =6.52,P =0.012).There were significant differences in APTI,and D-dimer between PVH-IVH and non-PVH-IVH infants(all P ＜ 0.05).Multivariate Logistic regression analysis showed that the related factors included gestational age,neonatal respiratory distress syndrome(NRDS),hyperoxia,and mechanical ventilation(OR =3.98,1.72,2.99,5.65,all P ＜ 0.05).The NBNA scores of the 2 groups were also significantly different(t =9.16,P ＜ 0.05).Conclusions The occurrence of PVH-IVH is very complex resulted from gestational age,NRDS,hyperoxia,mechanical ventilation and other factors.In order to avoid the occurrence of intracranial hemorrhage and improve the life quality of preterm infants,the NRDS was prevented as far as possible,coagulation abnormalities were managed positively and the mechanical ventilation was shortened.

Objective To study the efficacy of electroacupuneture combined with auricular acupoint seeds on cluster headache and its adverse reaction interfering.Methods A total of 98 cases with stroke were randomly recruited into a control group of 49 cases and a treatment group Of 49 cases.The control group was treated with conventional medicine,while the treatment group was treated with electro-acupuncture combined with auricular acupoint seeds,besides tge conventional intemal medicine.The efficacy was evaluated after one monm's treatment.A follow-up efficacy was evaluated in the continuous 6 months.Results 2 cases in the treatment group and 1 case in the control group quitted in the process.The treatment group showed significant better results in the immediate efficacy,3-mortth follow-up efficacy and 6-months follow-up efficacy as compared to the control group(all P＜0.05).The adverse reaction incidences of the treatment group were also significantly lower than the control grouD at 3-months and 6-months follow-up study(all P＜0.05).Conclusion The therapy of electro-acupuncture combined with auricular acupoint seeds is helpful to improve pharmacotherapy effect and alleviate medium term and long term adverse drug reaction.It can be served as an effective preventative measure for cluster headache.

Objective To investigate the effect of goserelin combined with marvelon in treatment of en -dometriosis(EM)and its effect on estrogen levels .Methods 120 EM patients were randomly divided into the ob-servation group(60 cases)and the control group(60 cases)according to the digital table.Two groups underwent laparoscopy .3.6 mg sustained release goserelin was injected subcutaneously from the anterior abdominal wall once every four weeks on the first time of menses recovery .The treatment continued during the following six months.The observation group were treated with marvelon on the basis of this , 1 tablet each day, 21 days of con-tinuous treatment and then began to take medicine on the 5 th day of the next menstruation during the following six months.The two groups were followed up for two years .The level of luteinizing hormone(LH), follicle-stimula-ting hormone(FSH)and estradiol(E2), the cumulative pregnancy rate, recurrence rate and peri menopausal sta-tus of the two groups were observed before and after treatment .Results The total effective rate was 96.67%and 83.33%respectively for the observation group and the control group .The difference had statistical significance (P<0.05).The level of E2, FSH, and LH at 1 month, 3 months, 6 months after treatmnet was lower than that before treatment(P<0.05).The level of E2, FSH, LH of two groups had no statistical difference (P<0.05). The cumulative pregnancy rate of the observation group was higher than that of the control group while the recur -rence rate was lower than that of the control group ( P<0.05 ) .The perimenopausal symptom score ( Kupperman score)of the two groups had no significant difference (P>0.05).Conclusion Goserelin combined with marvel-on can effectively improve the effect of laparoscopic surgery in treatment of endometriosis , lower estrogen level , improve patients'pregnancy rate , and reduce the recurrence rate .

Objective To investigate the protective effects of thalidomide on rats with acute necrotizing pancreatitis (ANP) and its mechanism. Methods Fifty four SD rats were randomly divided into the three groups; ANP group, thalidomide group and control group with 18 rats in each group. The model of ANP was induced by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct. Rats in thalidomide group received thalidomide 200 mg/kg body weight gastric lavage 1 h after ANP induction. The rats were sacrificed 3 h, 6 h, and 12 h after ANP induction, and the amount of intraperitoneal ascites was quantified. The serum levels of TNF-α, IL-6, IL-18 were measured by EUSA. The proportion of CD4 + T cell, CD8 + T cell in peripheral blood was determined by flow cytometry. The expression of TNF-α mRNA in pancreatic tissue were measured by RT-PCR. The expression of ICAM-1 protein in pancreatic tissue was measured by immunohistochemistry. Pancreatic tissue underwent pathologic examination. Results Six hours after surgery, the amount of ascite, serum levels of TNF-α, IL-6, IL-18, CD4 + T cell, CD,+ T cell, pancreatic TNF-α mRNA and ICAM-1 protein expression, pathologic score in control group was (1.03 ±0.31)ml,(57.17±11.29)pg/ml, (24.45 ±4.14)pg/ml, (64.23 ±21.85)pg/ml, (47.58 ±9.21)% , (40.88 ± 2.96)%, 0.07 ±0.02, 0.57 ±0.30, 0.67 ±0.81, respectively, and the corresponding values were (3.63 ± 0.38)ml, (107.54 ±33.05) pg/ml, (47.30 ± 11.40) pg/ml, (367.76 ± 108.43 ) pg/ml, (54.90 ± 7.15)%, (17.17 ±3.12)%, 0.65 ±0.26, 3.20 ±0.57, 11.50 ±1.87 in ANP group; and (1.45 ±0.53)ml, (80.60 ±20.48) pg/ml, (26.61 ±10.85) pg/ml, (321.82 ±85.20) pg/ml, (29.80 ±2.19)% , (15.52± 1.96)%, 0.35 ±0.23, 2.37 ±0.67, 8.00 ±3.03. Besides the value of CD8 + T cell was significantly decreased, all other values were significantly increased when compared with control group (P <0.05). Conclusions Thalidomide can decrease the release of inflammatory mediator, and reduce the pathological damage of pancreas of ANP rats by inhibiting TNF-α mRNA expression.

Objective To evaluate the effects of nutritional intervention on the incidence of malnutrition in patients on maintenance hemodialysis (MHD).Methods Totally 196 patients on MHD were randomly assigned to nutritional intervention group (n =98) and control group (n =98).Nutritional intervention or free diet were given in these two groups.The incidence of malnutrition in these two groups was recoded.Results The baseline age,biochemical indicators,and incidence of malnutrition were matched in these two groups (P ＞0.05).After 6 months,although the number of the patients with malnutrition [35.7％ (35/98)] was slightly increased in the intervention group,it showed no significant difference when compared with the baseline level [33.7％ (33/98),P ＞0.05].In the control group,however,the incidence of malnutrition [59.2％ (58/98)] significantly increased (P ＜0.05).The incidence of malnutrition was significantly lower in the intervention group than in the control group (P ＜ 0.05).Conclusion Nutritional intervention can lower the incidence of malnutrition in patients on MHD.

Objective To determine the effects of Hyperoside ( Hyp) on the memory impairment induced by hypobaric hypoxia. Meth-ods SD rats were exposed in hypobaric hypoxia chamber which simulated 6 100 m high altitude for 7 days to induce memory impairment. Morris maze was applied to determine the effects of Hyp on memory in hypobaric chamber. The activities of SOD and CAT and levels of GSH and MDA in rat hippocampus were measured by assay kit ( Nanjing Jiancheng Bioengineering Institute) . Results In target quadrant,the time and path and the frequency to cross platform were reduced under hypobaric hypoxia as compared with normoxia group ( P < 0. 05 or P<0. 01). Administration of Hyp significantly attenuated the hypoxia-induced the memory impairment responses(P<0. 05 or P<0. 01). The activities of SOD and CAT in rats hippocampus exposed to hypobaric hypoxia were significantly improved in the presence of Hyp as com-pared with the absence of Hyp(P<0. 05 or P<0. 01),so did GSH level (P<0. 05 or P<0. 01). Significant decrease of MDA levels was observed in the hippocampus on exposure to hypobaric hypoxia with Hyp as compared without Hyp. Conclusion Hyp reduces hypoxia-in-duced oxidative stress injury by upregulating activity of antioxidant proteins such as SOD,CAT in hippocampus of rats, which contribute to at-tenuate memory impairment induced by hypobaric hypoxia.

Objective To observe the effects of saponins of Panax notoginseng (PNS) on the brain energy metabolism and mitochondrial respiratory function of oxidative damaged rat model mimic to Alzheimer disease (AD). Methods Oxidative damaged rat model mimic AD (AD group) was established by injection of dihydroxy fumaric acid (DHF) and FeCl3 ADP into the left lateral ventricle of rats. The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and phosphate creatine (Pcr) in brain were determined by high performance liquid chromatography (HPLC). The mitochondrial functions (R3, R4, P/O, and OPR) were observed by Clark oxygen electrode method. The difference in the above parameters in rats in the control group (injection of saline), model group, and administration group (injection of PNS) was observed. Results The levels of ATP, Pcr, and R3, R4, P/O, and OPR of rats in AD group were significantly lower than those in the control group (0.9% NaCl instead of DHF FeCl3 ADP), but those in PNS group were higher than those in the AD group. Conclusion PNS can improve brain energy metabolism and mitochondrial function of oxidative damaged rats, suggesting that PNS could be used for the treatment of AD.