Objective To observe the clinical efficacy of plum-blossom needling combined with Deanxit for the treatment of depression or anxiety in chronic tension headache patients. Methods Sixty chronic tension headache patients with depression and/or anxiety were randomized into treatment group and control group , 30 cases in each group. The treatment group was treated by plum-blossom needling combined with oral use of Deanxit , and the control group was treated only by oral use of Deanxit. After treatment for 4 weeks , the scores of headache symptom, Hamilton Depressive Scale (HAMD), and Hamilton Anxiety Scale (HAMA) of the two groups were observed. Results After treatment , the scores of headache symptom , HAMA and HAMD in all patients were decreased (P < 0 . 05 compared with those before treatment), and the decrease in the treatment group was obviously superior to that in the control group, the difference being significant between the two groups (P <0.05). Conclusion Plum-blossom needling combined with Deanxit shows better effect than Deanxit for the treatment of depression or anxiety in chronic tension headache patients.

There is a close relationship between osteoporosis and osteoarthritis .The joints of bearing weight often suffer the osteoarthristis with osteoporosis .There are some symptoms in the disease ,such as pain and motion disorder ,and joint abnormality in late＋ stage .The principle of rehabilitation is to improve osteoporosis , release pain and increase the function if motion .The treatment includes medicament ,acupuncture ,injection ,rehabilitation ,psychology and surgery therapies .The injection can release pain effectiverly .The methods of rehabilitation include physical therapy,exercise ,massage ,and occupational therapy.These therapies can release pain ,improve motion and increase the quality of life .The article reveiws the clinical symptoms ,diagnosis and treatment ,especially the rehabilitation therrapy.

ABRO1 (Abraxas Brother 1), known also as KIAA0157 or FAM175B, is an important component of BRISC deubiquitinating enzyme ( DUB) complex which specifically shears K-63 linked polyubiquitin chains .This article reviews the research progress in ABRO 1 in terms of its structural characteristics , BRISC complex composition , the regulation of DUB activity and interferon response , oxidative stress response and anti-myocardial ischemia and provides new ideas on fur-ther study of the physiological function of ABRO 1 and its association with immune and cardiovascular diseases .

Objective To study the relationship of the polymorphism of endothelial nitric oxide synthase (eNOS) gene and blood pressure, lipid profiles and blood sugar level. Methods Totally 184 essential hypertension patients and 196 matched health individuals with normal blood pressure were subjected in this study. Their age, sex and BMI were recorded and eNOS Glu 298 Asp gene polymorphism were detected in using PCR-RFLP. Lipid profiles, including triglycerides (TG), total cholesterol (CHO), HDL, LDL, and blood sugar level were also detected. Results Significant difference of the percentage of eNOS Glu 298 Asp gene were observed in age, systolic blood pressure, BMI group (P

Objective:To analyze the distribution of HFE gene (hemochromatosis gene) mutation in normal and iron overload men and discuss the influence of HFE gene mutation on iron overload status of Chinese adult men..Method:The data of 226 normal and 331 iron overload men were drawn from National Nutrition and Health Survey in 2002 as control and case group respectively to analyze and compare the distribution of HFE mutation including C282Y mutation, H63D mutation and S65C mutation in normal and iron overload men.Results:In case group, normal gene was 90.03%, homozygosity for H63D mutation 0.6%, heterozygosity for H63D mutation 9.07%, heterozygosity for S65C 0.3%. In control group normal gene was 94.25%, heterozygosity for H63D 5.75%, and no S65C and C282Y mutation was detected. Conclusion: HFE gene mutation was not the major reason resulting in iron overload in Chinese adult men.

Objective:To study the critical value of soluble transferring receptor (sTfR) for assessing iron overload in Chinese adult men. Method:226 normal iron status and 331 iron overload men were drawn from National Nutrition and Health Survey 2002 as control group and case group respectively. To compare the sTfR concentration and distribution between two groups and ascertain the sTfR critical value to assess iron overload by ROC curve. Results:The concentrations of sTfR were 1 325.46?484.92 ng/ml, 1 788.56? 457.02 ng/ml in case group and control group respectively, the former lower than the latter (P

Objective To investigate whether the partially C-terminal deletion of NR2A subunit alters the surface expression and channel function of NMDA receptors in both HEK293 cells and cultured hippocampal neurons of rats. Methods Four plasmids for NR2A mutants with N-terminally GFP-tagged and C-terminal deletion NR2A?C1(?897L-1017S),NR2A?C2(?1024D-1142P),NR2A?C3(?1149D-1347G),and NR2A?C4(?1354S-1464V) were generated,and transfected into HEK293 cells and hippocampal neurons in culture.Surface staining was performed using anti-GFP antibody and Cy3 conjugated secondary antibody.Glutamate evoked currents were also detected using whole-cell recording. Results Positive surface staining was found for all the HEK293 cell co-transfected NR1-1a/NR2A?C1,NR1-1a/NR2A?C2,NR1-1a/NR2A?C3 or NR1-1a/NR2A?C4,and quantitative analysis showed no significant decrease in surface expression level when compared to that from NR1-1a/NR2A transfection.Glutamate-evoked currents were recorded in HEK293 cells co-transfected with NR1-1a/NR2A?C2 or NR1-1a/NR2A?C4.Surface expression level of NMDA receptor clusters on dendrites was significantly decreased in the neurons transfected with NR2A?C1,NR2A?C2 or NR2A?C3 than in those transfected with NR2A.Conclusion C-terminal deletion of NR2A subunit differentially effects surface expression of NMDA receptors in HEK293 cells and in hippocampal neurons in culture.

Objective To investigate changes in NF ?B signal pathway in PAM stimulated by lipopolysaccharide(LPS) in vitro , and to explore the molecular pathological mechanism of acute lung injury(ALI). Methods After PAM were stimulated by LPS, the changes in expression of IKK mRNA, activation of NF ?B, degradation of I?B, and secretion of TNF ? in PAM were measured at 0, 15min, 30min, 1h, 2h, and 4h by in situ hybridization, electrophoretic mobility shift assay (EMSA), and enzyme linked immune absorbing analysis (ELISA), respectively. Results The expression of IKK ? mRNA was increased 15min after LPS stimulation and reached the peak at 30 min, then returned to the base line after 1 hour. The changes in I?B ? mRNA were opposite. The activity of NF ?B was increased 15min after LPS stimulation, peaking at 1 hours, and returned to the pre stimulation level after 2 hours. The content of TNF ? was increased initially at 30min, reached the peak at 1 hour, and gradually returned to the pre stimulation level in 2～4 hour. Conclusion The transduction pathway of activation of IKK ? degradation of I?B/activation of NF ?B/synthesization of TNF ? might play a critical role in the molecular pathological mechanism of LPS induced ALI.

[ ABSTRACT ] AIM: To investigate the molecular mechanisms of cytotoxicity induced by dihydroartemisinin ( DHA) in non-small cell lung cancer ( NSCLC) cells.METHODS:NSCLC cell lines A549 and NCI-H1650 were treated with various concentrations of DHA for indicated time.Subsequently, the effects of DHA on the cell activity, colony forma-tion ability and apoptosis were determined by MTT assay, colony formation assay, Annexin V-FITC/PI staining and flow cy-tometry, respectively.At the same time, the effects of DHA on glucose, ATP and lactate levels were assessed, and the PI3K pathway activation and glucose transporter 2 ( GLUT2) expression were detected by Western blot in the A549 cells and NCI-H1650 cells.Overexpression of GLUT2 and Rheb was established in A549 and NCI-H1650 cells by transfection with GST-GLUT2 and GST-Rheb plasmids, respectively, and the effects of DHA on cell activity, apoptosis, glucose level, ATP content and PI3K pathway activation were analyzed in A549 cells and NCI-H1650 cells.The effect of glucose depriva-tion on the cytotoxicity triggered by DHA in NSCLC cells was also determined.RESULTS:Compared with control group, DHA significantly inhibited cell activity and colony formation ability, and induced remarkable cell apoptosis in the A549 cells and NCI-H1650 cells.At the same time, DHA reduced ATP and lactate contents, and hindered glucose uptake in a time-and dose-dependent manner in A549 cells and NCI-H1650 cells.The activity of PI3K pathway and GLUT2 expression were downregulated, while upregulated GLUT2 expression and activated PI3K pathway reduced the cytotoxicity induced by DHA in NSCLC cells.Glucose deprivation increased DHA-mediated cytotoxicity in NSCLC cells.On the contrary, high levels of glucose inhibited DHA-mediated cytotoxicity in NSCLC cells.CONCLUSION: DHA restrains cell activity and colony formation, and induces apoptosis.DHA induces cytotoxicity via inhibiting PI3K pathway activation and GLUT2 ex-pression, leading to inhibit glycolytic metabolism in NSCLC cells.