Objective: To establish the quality standard for Shentaipikang Granules (Cornu Cervi Pantotrichum, Herba Epimedii, Herba Cistanches, Cortex Phellodendri, etc). Methods: Counu Cervi Pantotrichum in this prescription was examined by microscopical identification. Cortex Phellodendri, Rhizoma Dioscoreae and Rhizoma Atractylodis Macrocephalae were identified by TLC. Radix Rehmanniae was identified by HPLC. Icariin was determined by HPLC. Results: Cornu Cervi Pantotrichum could be examined by microscopical identification, Cortex Phellodendri, Rhizoma Dioscoreae and Rhizoma Atractylodis Macrocephalae by TLC, and Radix Rehmanniae by HPLC. Icariin showed a good linear relationship at a range of 0.104 ～ 0.624 ?g , r = 0.9998 . The average recovery was 100.1% and RSD was 1.3% ( n =9) respectively. Conclusion: These methods are simple, accurate and specific and can be used for the quality control of Shentaipikang Granules.

Objective To investigate the effect of mesenchymal stem cells transplatation on angiogenesis and secretion of cytokines after rat myocardial infarction. Methods Harvested rat mesenchymal stem cells were cultured and labeled in vitro. And at the same time, myocardial infarct model was set up by liquid nitrogen cryoinjuring the left ventricular free wall. 4 weeks later, 2?10 6 mesenchymal stem cells or cold D-Hanks liquid were injected into several different points of infarcted area. At 1, 2 and 4 weeks after mesenchymal stem cells transplatation, specimens were acquired from infarcted area. Then cell morphology and capillary density were measured, and vascular endothelial growth factor(VEGF) and transforming growth factor ?(TGF-?) expression levels were assayed by RT-PCR. Results Mesenchymal stem cells were alive at 1, 2 and 4 weeks after transplantation and had a trend toward differentiation and maturation in vivo. The number of capillary vessels in per infarcted area (0 1 mm 2) in experiment group increased compared with control group. And the number of capillary vessels in experimental group had a trend to decrease in experiment group(P

Objective To investigate the effect of mesenchymal stem cells transplantation on heart function and the expression of Ca 2+ -ATPase (ERCA) mRNA after rat myocardial infarction. Methods Wistar inbred rat mesenchymal stem cells were cultivated, proliferated and labeled in vitro. Myocardial infarct models were set up by liquid nitrogen cryoinjuring the free wall of left ventricle. 4 weeks later, 2?10 6 mesenchymal stem cells or cold D-Hanks solution were injected into several different points of infarcted myocardial area of experiment group and control group rats. In addition, 5 Wistar inbred rats served as normal group only got thoracotomy twice. 4 weeks after transplantation, all rats received cardiac function test and specimens from infarcted area were got for the expression assay of SERCA2 mRNA by RT-PCR. Results Mesenchymal stem cells were alive at 1, 2 and 4 weeks after transplantation and had a trend toward differentiation and maturation in vivo. Traumatic dynamic measurement was applied to assay heart function by Buxco system, which showed that SLVP and +dp/dt were the highest in normal group, next in experiment group, and the lowest in control group, which LVEDP was the lowest in normal group, next in experiment group, and the highest in control group. The expression level of SERCA2 mRNA in control group was much lower than that in normal group(0.988?0.004 VS 1.824?0.009,P

To improve the precision of image registration based on corner detection, a relative position function between multiple points to determine matching points accurately. First the corners in images are detected using Harris detector, and clustering method is used to eliminate most wrong matches after coarse screening. Then the proposed relative position function is used as a criterion of precise matching. Finally the image registration process is accomplished by affine transformation. Results show that the proposed algorithm is more effective and accurate than conventional registration algorithm.

Objective To explore the pertinent communication strategy of health education for parasitic diseases.Methods A cross-sectional study was carried out.Before the health education intervention,the knowledge,attitude and practice(KAP)for parasitic diseases of 199 and 250 farmers in two provinces of Sichuan and Yunnan in southwest China were investigated with questionnaire by using the method of PPS.Results Most of the farmers used the simple and crude toilets and their health status were poor,and there were only 7.57% of people having the bio-gas facilities.The awareness rate of the knowledge on malnutrition caused by parasitic diseases was 34.30% and the awareness rate of knowledge about anemia caused by parasitic diseases was 29.62%.There were 81.74% of farmers who believed helminthic drug and 74.61% of people who tended to buy drug by themselves.The health knowledge of the residents mainly came from the interpersonal communication and TV,and the farmers preferred TV,poster and brochure to get the health knowledge.Conclusion The health education with posters,TV and intervention communication is one of the important measures to control parasitic diseases.

were obviously increased. Conclusion HIF-1α was successfully cloned. HIF-1α-pcDNA3.1 can be effectively transfected into MSCs with liposome-mediated method, which can result stable expression of HIF-1αin transfected MSCs.

Objective To explore the relationship between the expression of basic fibroblast growth factor (bFGF) and microvessel density(MVD)in non-small cell lung cancer.Methods The expression of bFGF and MVD were observed in 54 eases of NSCLC were detected with in situ hybrldization and immunohistochemical detection.Resuits The expression of bFGF and MVD was greater in adenecarcinomas than in squamous cell carcinomas of NSCLC (P＜0.05).The expression of bFGF was significantly different among the three groups of both squamous cell carcinomas and adenocarecnomas with varying differentiation (P＜0.05).There was hisher bFGF expression and greater MVD in NSCLC patients with regional lymplmode involvement and those with laterdistant metastasis(P＜0.05).Condusion bFGF may play an important role in tumor angiogenesis and lymphatic metastasis of human NSCLC,and detection of bFGF may be a good metastasis and prognostic predictors for human NSCLC.

BACKGROUND:Standard artificial prosthesis does not well match to patients' skeleton due to absolute individuation,while the difference between them does not keep the stabilization of artificial joint for a long term.OBJECTIVE:To construct three-dimensional models of custom-made femoral prosthesis by using computer-aided design and to verify whether custom-made femoral prosthesis is superior to popular femoral prosthesis via simulating and contrasting mechanical study.DESIGN,TIME AND SETTING:Opening study was performed in the Orthopaedics Institute of the First Clinical Hospital and the Biomechanics Institute,Jilin University between September 2006 and May 2007.MATERIALS:One fresh femur was derived from adult corps.METHODS:Two-dimensional images of the fresh femur was obtained by using total-length CT scanning and processed with computer edge recognition and three-dimensional contour extraction software to identify outer and inner contour of bone cavity,extract contour data of bone cavity and prosthesis,and construct three-dimensional models of femur and custom-rhade femoral prosthesis.SolidWorks software was used to establish three-dimensional prosthetic models in common biological and bone cement types; moreover,bone-cement,biological,and custom-made femoral prosthesis were replaced via simulating clinical surgery.MAIN OUTCOME MEASURES:Standing on single or double feet was simulated to measure stress distribution,interface stress,and primary micromotion of femoral prosthesis in the three types.RESULTS:Edge extracting was replaced by Canny calculator,characterizing by stable running,credible outcome,and consistent with the primary request.Stress,femoral stress,interface stress,and primary micromotion of custom-made femoral prosthesis were significantly lower than biological and'bone-cement femoral prosthesis (P ＜ 0.01).CONCLUSION:The computer-aided design is reliable to perform the assistant design of prosthesis; furthermore,biomechanical properties of the custom-made femoral prosthesis are superior to those of popular femoral prosthesis.

Objective To discuss the reasons and the best treatment of the encephalocele during severe head injury. Method Retrospective analysis 26 cases of the clinical data of patients with acute encephalo-cele during severe head injury used bilateral craniotomy method. Result Good recovery in 4 patients, mod-erate disability in 5 patients, severe disability in 2 patients, vegetative state in 3 patients, death in 12 pa-tients. Conclusion Delayed intracranial hematoma and acute diffuse brain swelling are the main reasons of craniotomy brain injury in acute encephalocele, and using bilateral craniotomy can significantly increase the survival rate and quality of life.