Objective To explore the pertinent communication strategy of health education for parasitic diseases.Methods A cross-sectional study was carried out.Before the health education intervention,the knowledge,attitude and practice(KAP)for parasitic diseases of 199 and 250 farmers in two provinces of Sichuan and Yunnan in southwest China were investigated with questionnaire by using the method of PPS.Results Most of the farmers used the simple and crude toilets and their health status were poor,and there were only 7.57% of people having the bio-gas facilities.The awareness rate of the knowledge on malnutrition caused by parasitic diseases was 34.30% and the awareness rate of knowledge about anemia caused by parasitic diseases was 29.62%.There were 81.74% of farmers who believed helminthic drug and 74.61% of people who tended to buy drug by themselves.The health knowledge of the residents mainly came from the interpersonal communication and TV,and the farmers preferred TV,poster and brochure to get the health knowledge.Conclusion The health education with posters,TV and intervention communication is one of the important measures to control parasitic diseases.

Objective To compare the therapeutic effects of three therapeutics to earlier primary gastric diffuse large B cell lymphoma(PG-DLBCL).Methods By reviewing a data-base of 66 patients with earlier PG-DLBCL from Aug 2000 to Oct 2011 were enrolled,patients were divided into chemotherapy alone(21 cases),radiotherapy combined with chemotherapy(22 cases) and surgery followed chemotherapy group(23 cases),and to comparing the prognosis of the three therapeutics.Results The complete remission rates(CR) of the three groups was 47.6％,77.3％,65.2％ (P ＞ 0.05).The median progression-free survival(PFS)of the three groups in 66 patients was 61.5,90.4 and 79.1 months,the radiotherapy combined group was proven to be significantly better than chemotherapy alone group and surgery followed chemotherapy group(x2 =6.726,P ＜0.05).The 3-year survival rate of the three groups was 81.6％,90.5％ and 82.6％.The five year survival rate was 72.5％,90.5％,82.6％.There was no significant difference (P ＞ 0.05) in 3-year survival rate and 5-year survival rate.Conclusions Radiotherapy combined with chemotherapy can significantly improve the PFS without increasing the risk of other adverse outcomes,and could be the first treatment way for the earlier PG-DLBCL.

Objective To comparison between radiofrequency ablation introduced by tri-dimension reconstruction and ultrasound and operation for the treatment of small hepatocellular carcinoma. Methods 94 patients with small hepatocellular carcinoma from Jan 2009 to Mar 25 in Shanxi Cancer Hospital were analyzed. 45 patients were both given CT examination to rebuild tri-dimension and radiofrequency ablation treatment. 49 patients were given excision. Both groups were given CT examination to rebuild tri-dimension before the treatment. The differences in recurrence rate, overall survival and complication ratio between the two groups were compared. Results After 1, 2 and 3 years radiofrequency ablation treatment, the survival rate in radiofrequency ablation group were 95.56 % (43/45), 86.67 % (39/45), 60.00 % (27/45) respectively, comparing with 93.88%(46/49), 79.60%(39/49), 59.20%(29/49) in operation group. The difference was not statistically significant (P>0.05). There was no statistical significance in 1-year, 2-year and 3-year recurrence rates between the two groups (P> 0.05). There were statistical significances in incidence of post-treatment pains [13.33%(6/45) vs 100.00%(49/49),χ2=60.416, P<0.05] and complication (P<0.05) between the two groups. By Mar 25th, 2015, there was no needle tract implantation in the patients with radiofrequency ablation treatment. Conclusions For the small hepatocellular carcinoma with less than 3cm in diameter, introduced by CT tri-dimension image reconstruction and ultrasound, the radiofrequency ablation treatment excels in effect with fewer damages, infective complications, lower cost and can be applied many times. The overall effect is close to surgical removal, and therefore it can be used as the first line therapy for small hepatocellular carcinoma.

Objective To investigate the difference between the self-evaluation of patients with dental and facial esthetics in skeletal malocclusion and normal persons.Methods The subjects were divided into three groups:62 patients with skeletal Class Ⅱ,84 patients with skeletal Class Ⅲ,as well as 130 persons in control group.There was no obvious difference in gender and age among the three groups.Happiness with dental and facial appearance was assessed using questionnaires.Analysis of variance,linear regression and logistic regression were used for statistical analysis.Results Skeletal malocclusion patients were less happy with their dental appearance than control group.Class Ⅱ patients and women had lower happiness scores for their dental appearance.Among skeletal malocclusion patients,the shape (44.5 ％) and prominence of their teeth (17.8 ％) were the most frequent causes of concern.Older subjects,women,and skeletal malocclusion patients were less happy with their facial appearance.Most of them were dissatisfied with the facial appearance focused on the chin (53 ％),facial contours (37 ％),and the position of the teeth when smiling (33 ％).A greater proportion of Class Ⅲ subjects than Class Ⅱ subjects wished to change their appearance.Conclusions The findings indicate that women and skeletal malocclusion patients have lower levels of happiness with their dentofacial appearance.Although Class Ⅱ patients exhibit the lowest levels of happiness with their dental appearance,there is some evidence that concerns and awareness about their facial profile are more pronounced among the Class Ⅲ patients.

Objective To investigate the causes of success or failure of natural orifice translumenal endoscopic surgery(NOTES)in animals.Methods Data were collected from animal studies of NOTES carried out between August 2007 and September 2009.The studies were divided into acute phase and survival phase.In the studies,simple NOTES and laparoscopic-assisted NOTES were performed on female miniswines or adult mongrel dogs.Diagnostic abdominal exploration,liver biopsy,cholecystectomy,tubal ligation,oophorectomy,gastrojejunal anastomosis,and splenectomy were made in the animals.The intra-and post-operative complications and technical difficulties were recorded during the procedures.Results Totally 37 miniswines and 16 mongrel dogs were enrolled in the studies of NOTES procedures(91 procedures),including 22 acute experiments and 31 survival experiments.Simple NOTES was conducted through the transgastric route in 25 cases,transcolonic route in 8 cases,and combinations of transgastric and transcolonic routes in 16 cases,and combinations of transgastric and transvaginal routes in 6.In Laparoscopic-assisted cases,36 animals were treated via the transgastric route by traditional(26 cases)or single-port transumbilical laparoscopy(10 cases).Diagnostic exploration was carried out in 33 cases,liver biopsy was done in 11 cases,tubal ligation was made in 11,and oophorectomy was performed on 6;the procedures were all completed in these cases showing a success rate of 100%.Cholecystectomy was completed in 27 cases with a success rate of 44.4%(12/27).One case of splenectomy,and two cases of gastrojejunal anastomosis failed.The totally complication rate in the studies were 39.6%(21/53).The complications included perforation(8 cases),organ injury(5 cases),bleeding(4 cases),infection(3 cases)and pneumoperitoneum with hypertonia(1 case).Conclusions NOTES is feasible,safe and effective in diagnostic abdominal exploration and simple abdominal surgeries,but does not suit difficult abdominal surgeries.By using laparoscopy,the technique may be further developed and used in the future.

Objective To investigate into the indications and related anatomic foundation of transjugular intrahepatic portosystemic shunt(TIPS) creation by direct transcaval approach in patients with portal hypertension cirrhosis suffering unusual anatomy between the hepatic veins and portal bifurcation; and to evaluate the security, feasibility and clinical significance. Methods Direct transcaval approach TIPS were performed in 65 patients including active variceal bleeding (n=52), intractable ascites (n=12), and as a bridge to liver transplantation (n=1). Results Technical and functional success were achieved in all patients. The success rate was 100% without related complications including the technique and primary patency rate is obvious higher than classical TIPS. Conclusion In patients with unusual anatomy between the hepatic veins and portal bifurcation, and inaccessible or inadequate hepatic veins, transcaval TIPS creation is secure and feasible. The results suggest that the direct transcaval approach offering favorable primary patency because the shunt has a straight line in construction.

OBJECTIVE:To establish an HPLC method for the determination of the content of glucose in Glucose/Sodium Chloride injection.METHODS:The determination was performed on Waters Sugar PakⅠ column with column temperature set at 80 ℃,water as mobile phase and injection volume of 20 ?L.The cell temperature of RID detector was set at 40 ℃.RESULTS:The linear range of glucose was 0.05～2.50 mg?mL-1(r=0.999 9) with the lowest detection limit of 24 ng.The average recovery ranged from 99.80% to 99.90%(RSD=0.21%).CONCLUSION:The method is simple,rapid,accurate,sensitive and reliable in operation,and it is applicable for determination of glucose in Glucose/Sodium Chloride injection.

OBJECTIVE: To establish an HPLC method with pre-column programming derivatization for the content determination of 5 kinds of amino acids in Compound ?-ketoacid tablets.METHODS: The separation was performed on C8 column(gradient elution) with column temperature at 40 ℃.OPA was used as the derivation reagent.The detection wavelength was set at 338 nm.The contents of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine in Compound ?-ketoacid tablets were determined.RESULTS: The linear ranges of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine were 0.10～5.25 mg?mL-1(r=0.999 9),0.052～2.65 mg?mL-1(r=0.999 8),0.022～1.15 mg?mL-1(r=0.999 9),0.038～1.91 mg?mL-1(r=0.999 7),0.030～1.51 mg?mL-1(r=1.000 0),respectively.The average recovery was 99.8%～100.1%(RSD=0.1%).CONCLUSION: This method is exclusive,sensitive and reliable for the content determination of amino acids in Compound ?-ketoacid tablets.

Purpose:To explore different functions and values of Livin ? and Livin ?-specific silencing in inducing SPC-A1 cell apoptosis, respectively. Methods:First, Livin ?+?, Livin?and Livin ?-specific siRNA were steadily expressed in SPC-A1 respectively. Next, after isoform-specific gene silencing, biological changes with regard to cell morphology and proliferation of lung cancer cell were observed. Then, TUNEL and FCAS were performed to test the rate of apoptosis. Lastly, statistical analysis was performed to explore the different functions and values of isoform-specific gene silencing in inducing lung cancer cell apoptosis.Results:After gene silencing of Livin ?+?, Livin ? and Livin ?,there was an evident decrease in colony forming ability of SPC-A1 compared with the control(P