Objective To discuss the effects of bacteria lysates (OM-85BV),1,25-dihydroxy vitamin D3 [1,25-(OH)2-VitD3],two immune regulators on airway inflammation in asthmatic mice models,and its pathways of action on airway inflammation were discussed.Methods Forty male BALB/c mice graded 4-6 weeks were randomly divided into 5 groups.Group A:control group;group B:asthmatic model group;group C:1,25-(OH)2-VitD3 group;group D:OM-85BV group;group E:combination group.On days 0 to 14,mice in C,D and E groups were given 1,25-(OH)2-VitD3,OM-85BV and 1,25-(OH)2-VitD3 + OM-85 BV,and mice in A,B groups were given 9 g/L saline instead.On days 15,22 and 29,mice in B,C,D,E groups were intraperitoneally with injection of ovalbumin(OVA)-aluminum hydroxide [Al(OH)3].Group A were given 9 g/L saline instead.On days 36 to 40,mice of B,C,D,E groups were given an aerosol challenge of 10 g/L OVA for 0.5 h once a day.Mice in the control group were given the same amount of 9 g/L saline.Animals were sacrificed 24 hours after the final inhalational challenge,and for the recovered bronchoalveolar lavage fluid(BALF) of the left lung was used for differential inflammatory cell counts and for detecting the level of inflammatory cytokine interleukin-17 (IL-17).Right lung samples were used for pathological investigation and detecting the expression of the IL-17 mRNA and RORγt mRNA by real time-PCR.Results Compared with the control group,the asthma models expressed more serious expression in bronchospasm contraction,hyperplasia disorders of bronchial epithelial cells,infiltration of inflammatory cells in lung,and so on.Compared with the control group,the total number of inflammatory cells counts[(104.04 ±5.51) 107/L vs (22.79 ± 1.91) 107/L] and eosinophils proportion [(37.63 ± 3.64) ％ vs (2.37 ± 1.55) ％] in BALF in group B were significantly increased (all P ＜ 0.05),the levels of IL-17 [(85.13 ± 5.77) 103 pg/L vs (47.44 ± 4.57) 103 pg/L] in BALF were significantly higher(P ＜ 0.05),the relative expressions of IL-17 mRNA (13.68 ± 1.59 vs 1.00 ± 0.00) and RORγt mRNA (4.53 ± 0.51 vs 1.00 ± 0.00) in lung were higher,which had a statistical significance (all P ＜ 0.05).The situations of group C,D,E were obviously improved compared with group B,and those of group D were improved remarkably.Conclusions Oral OM-85BV and 1,25-(OH)2-VitD3 intervention could relieve the airway inflammation of asthmatic mice models,and its effect can be remarkable by oral OM-85BV.The two immune regulators could relieve the degree of airway inflammation on asthmatic mice models by reducing the expression of Th17 cells differentiation.Therefore the two immune regulators could be the choices for preventing the happening and the development of the asthmatic airway inflammation.

Objective To explore the effect of vascular endothelial growth factor-C (VEGF-C) gene transfection on the expression level of VEGF-C in human breast cancer MCF-7 cell. Methods The constructed VEGF-C gene eukaryotic expression vector was transfected into the human breast cancer MCF-7 cell by using lipofectamine transfection reagents, and the positive cell clones were obtained through G418 selection after transfection. The expressions of VEGF-C mRNA and protein were detected by RT-PCR and Western blot respectively. Results Following the transfection of the VEGF-C recombination plasmid, there were significant differences on the expression levels of VEGF-C mRNA and protein between pcDNA3.1-VEGF-C transfection group and pcDNA3.1 transfection group (12.382?2.183 vs 6.039?1.950, P

Objective To study the feasibility and curative effect of laparoscopic radical resection of rectal carcinoma. Methods Twenty-eight cases undergoing laparoscopic radical resection (laparoscopic group) and 26 cases undergoing open radical resection (open group) were enrolled from January 2004 to December 2007. The following parameters: operation-related situations, postoperative recovery,result of radical resection, and postoperative outcome were compared between the two groups. Results The blood loss during operation in laparoscopic group was less than that in open group [(148.0±26.5) ml vs (396.0±79.6) ml, P<0.01]. The gastrointestinal tract and urination function in laparoseopic group recovered faster than those in open group[the time of diet in taking was (2.8±0.1) d vs (3.9±0.3) d,the time of detaining urethral catheter was(4.2±0.2) d vs (6.0±0.8) d] (P<0.05). The hospital stay was shorter in laparoscopic group than that in open group [(9.8±1.1) d vs(13.2±2.8) d, P<0.01]. The operation time, the number of cleared lymph nodes and complications of laparoscopic between the two groups were no significant difference (P>0.05). There was no significant difference in local recurrent and beyond metastases rates between the two groups. Conclusion Laparoscopic radical resection of rectal carcinoma parallels with open surgery is in safety and effectiveness, it has less traumatic and blood loss and faster in patients recovery.

Stomatology is an applied science,and experimental teaching plays an important role in training renovation talents.A complete construction of the laboratory system is a complex systematic project,which requires the right teaching conception,advanced experimental instruments,high-quality teachers of laboratory,the optimization of the teaching contents,as well as highly efficient operating mechanism and management approach.It is also a process of exploration and innovation.

BACKGROUND: It has reported that nicotinamide is capable of protecting intervertebral disc (IVD) against interleukin-1β (IL-1β) or tumor necrosis factor-alpha (TNF-α) induced degeneration. However, the protective mechanism of nicotinamide on IVD cells apoptosis and proliferation remains unclear.OBJECTIVE: To investigate regulatory effects of nicotinamide on rabbit nucleus pulposus cell apoptosis and proliferation in vitro.DESIGN, TIME AND SETTING: Randomized control grouping design, which was carried out in the Laboratory of Orthopaedics and Stem Cell Center, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology from April to October 2007.MATERIALS: Ten Japanese white rabbits (aged 2-3 months weighing 1.5-2.0 kg) were used in this study. Furthermore, nucleus pulposus cells obtained from L1-6 lumbar spine were harvested and cultured for further experiments.METHODS: The NP cells were divided into 6 groups, including control group (without any drug as control), nicotinamide group (0.5 g/L nicotinamide), IL-1β group (10 μg/L IL-1β), IL-1β + caspase group (10 μg/L IL-1β and non-specific caspase inhibitor Z-VAD-FMK), IL-1β + small-dose nicotinamide group (10 μg/L IL-1β and 0.05 g/L nicotinamide), and IL-1β + large-dose nicotinamide group (10 μg/L IL-1β and 0.5 g/L nicotinamide). After 3 days of culture, the cells were examined with Annexin V-PI staining, caspase-3, 8 and 9 activity staining and MTT assay.MAIN OUTCOME MEASURES: The apoptotic rates, the positive rates of caspase-3, 8 and 9 activity staining and the absorbance of MTT assay of each group.RESULTS: ① As compared to IL-1β group, the apoptotic rates were decreased in the IL-1β + caspase group and IL-1β + large-dose nicotinamide group (P < 0.01). ②As compared to IL-1β group, the positive rates of caspase-3, 8 and 9 activity staining were decreased in the IL-1β + caspase group, IL-1β + large-dose and small-dose nicotinamide groups (P < 0.01 or P < 0.01). ③As compared to IL-1β group, the absorbance was increased in the IL-1β + caspase group and IL-1β + large-dose nicotinamide group (P < 0.01).CONCLUSION: Nicotinamide is capable of promoting cell proliferation and inhibiting IL-1β induced apoptosis of nucleus pulposus cells in vitro. The inhibition of apoptosis mainly acts via inhibition of the mitochondrial pathway.

Objective:To evaluate the measurement uncertainty in the determination of sodium valproate tablets by GC with an in -ternal standard method , and determine the main sources of uncertainty .Methods:A GC internal standard method was selected to sys-tematically analyze the uncertainty in the determination of sodium valproate tablets , including the sample quantity , dilution ratio, purity and area repeatability of chromatographic peaks .Results: The expanded uncertainty of sodium valproate tablets was 2.7%, and the determination range of sodium valproate tablets was (96.3 ±2.7)%(k=2).Conclusion:The established GC internal standard meth-od for the uncertainty evaluation is reliable , which is helpful to improving the quality evaluation and control of sodium valproate tablets .

BACKGROUND:Studies have found that Wudi Dan can suppress local inflammatory response of the lesioned joints to protect the articular cartilage. OBJECTIVE:To verify the effect of Wudi Dan on chondrocyte viability and apoptosis as wel as the therapeutic effect on osteoarthritis. METHODS:Rat chondrocytes were cultured in serum medium containing Wudi Dan, and the effects of Wudi Dan on cel viability and apoptosis were observed by comparison with the control group. Rabbit model of knee osteoarthritis was constructed using modified Hulth method. Rabbit models were divided into two groups:Wudi Dan group treated with Wudi Dan and control group treated with normal saline, twice a day, consecutively for 4 weeks. Therapeutic effect of Wudi Dan on knee osteoarthritis was observed;cel viability and apoptosis were observed under microscope;the levels of interleukin-1 and matrix metal oproteinase-3 were determined using immunohistochemical method. RESULTS AND CONCLUSION:The apoptotic rate of chondrocytes was significantly lower in the Wudi Dan group than the control group. Pathological findings of the rabbit knee joints showed that the control group had more severe damage to the articular cartilage than the Wudi Dan group. Immunohistochemical staining revealed that in the Wudi Dan group, the cytoplasm and extracel ular matrix were colored light and there were a smal number of positive cel s as wel as low expression of interleukin-1 and matrix metal oproteinase-3. The results suggest that Wudi Dan can effectively protect against articular cartilage lesions, reduce inflammation, and have a good therapeutic effect on osteoarthritis. Its mechanism may be related to inhibition of chondrocyte apoptosis, reduction of cytokine production and inhibition of protein expression of matrix metal oproteinase.

Objective To analyze the imaging features of primary pulmonary hemangiopericytoma,and to provide a helpful method for clinical diagnosis.Methods Retrospectively analyze the CT and MRI characteristics of 3 cases with pathologically proved primary pulmonary hemangiopericytomas.Results All cases manifested as solitary massive tumors.On plain CT scans,the tumors were heterogeneous,well and sharply circumscribed.On contrast-enhanced CT scans,the tumors were apparently enhanced and the abnormally enhanced vasculars could be seen.On MRI scans,the tumors had inhomogeneous signal intensity and the vascular flowing-void effect could be seen.Conclusion Solitary tumor of massive volume,abnormally enhanced vasculars on contrast-enhanced CT and the vascular flowing-void effect on MRI are imaging features of primary pulmonary hemangiopericytomas.

OBJECTIVE:To investigate the similarity of dissolution curves between generic preparations and reference prepara-tions of Bisacodyl enteric-coated tablets in various dissolution mediums,and to provide reference for improving production technolo-gy and internal quality of generic preparations. METHODS:Paddle method was adopted with rotation speed of 75 r/min. The disso-lution test was performed using 1000 mL pH 6.0 phosphate buffer solution,pH 6.8 phosphate buffer solution,water containing 2% sodium lauryl sulfate. HPLC method was used to determine average accumulative dissolution of main components from 3 kinds of generic preparations and reference preparations at different time points to draw out dissolution curves. Similarity factor(f2)meth-od was used to the similarity of dissolution curves. RESULTS:Dissolution curves of reference preparations were basically the same in 3 kinds of dissolution mediums. But the dissolution curve f2 of one generic preparation among 3 manufactures to dissolution curve of reference preparation were ≥50,namely the similarity. CONCLUSIONS:The quality of generic Bisacodyl enteric-coat-ed tablets produced by different manufacturers is obviously different;the generic preparations needs to be further improved in the production technology and internal quality. For domestic generic preparation,it is necessary to strengthen the real-time monitoring of its dissolution curve,to ensure the drug quality.

Since the development of civil basic medical insurance, it exerts subtle influence on medical conduct. The medical insurance economically affects the medical conduct. And medical ethics is one of the important factors which influence medical conducts greatly. Therefore, it is very important for the medical insurance enterprise's development to discipline the medical conducts by medical ethics.