Objective To study the feasibility and curative effect of laparoscopic radical resection of rectal carcinoma. Methods Twenty-eight cases undergoing laparoscopic radical resection (laparoscopic group) and 26 cases undergoing open radical resection (open group) were enrolled from January 2004 to December 2007. The following parameters: operation-related situations, postoperative recovery,result of radical resection, and postoperative outcome were compared between the two groups. Results The blood loss during operation in laparoscopic group was less than that in open group [(148.0±26.5) ml vs (396.0±79.6) ml, P<0.01]. The gastrointestinal tract and urination function in laparoseopic group recovered faster than those in open group[the time of diet in taking was (2.8±0.1) d vs (3.9±0.3) d,the time of detaining urethral catheter was(4.2±0.2) d vs (6.0±0.8) d] (P<0.05). The hospital stay was shorter in laparoscopic group than that in open group [(9.8±1.1) d vs(13.2±2.8) d, P<0.01]. The operation time, the number of cleared lymph nodes and complications of laparoscopic between the two groups were no significant difference (P>0.05). There was no significant difference in local recurrent and beyond metastases rates between the two groups. Conclusion Laparoscopic radical resection of rectal carcinoma parallels with open surgery is in safety and effectiveness, it has less traumatic and blood loss and faster in patients recovery.

Stomatology is an applied science,and experimental teaching plays an important role in training renovation talents.A complete construction of the laboratory system is a complex systematic project,which requires the right teaching conception,advanced experimental instruments,high-quality teachers of laboratory,the optimization of the teaching contents,as well as highly efficient operating mechanism and management approach.It is also a process of exploration and innovation.

Objective To explore the effect of vascular endothelial growth factor-C (VEGF-C) gene transfection on the expression level of VEGF-C in human breast cancer MCF-7 cell. Methods The constructed VEGF-C gene eukaryotic expression vector was transfected into the human breast cancer MCF-7 cell by using lipofectamine transfection reagents, and the positive cell clones were obtained through G418 selection after transfection. The expressions of VEGF-C mRNA and protein were detected by RT-PCR and Western blot respectively. Results Following the transfection of the VEGF-C recombination plasmid, there were significant differences on the expression levels of VEGF-C mRNA and protein between pcDNA3.1-VEGF-C transfection group and pcDNA3.1 transfection group (12.382?2.183 vs 6.039?1.950, P

Objective To discuss the effects of bacteria lysates (OM-85BV),1,25-dihydroxy vitamin D3 [1,25-(OH)2-VitD3],two immune regulators on airway inflammation in asthmatic mice models,and its pathways of action on airway inflammation were discussed.Methods Forty male BALB/c mice graded 4-6 weeks were randomly divided into 5 groups.Group A:control group;group B:asthmatic model group;group C:1,25-(OH)2-VitD3 group;group D:OM-85BV group;group E:combination group.On days 0 to 14,mice in C,D and E groups were given 1,25-(OH)2-VitD3,OM-85BV and 1,25-(OH)2-VitD3 + OM-85 BV,and mice in A,B groups were given 9 g/L saline instead.On days 15,22 and 29,mice in B,C,D,E groups were intraperitoneally with injection of ovalbumin(OVA)-aluminum hydroxide [Al(OH)3].Group A were given 9 g/L saline instead.On days 36 to 40,mice of B,C,D,E groups were given an aerosol challenge of 10 g/L OVA for 0.5 h once a day.Mice in the control group were given the same amount of 9 g/L saline.Animals were sacrificed 24 hours after the final inhalational challenge,and for the recovered bronchoalveolar lavage fluid(BALF) of the left lung was used for differential inflammatory cell counts and for detecting the level of inflammatory cytokine interleukin-17 (IL-17).Right lung samples were used for pathological investigation and detecting the expression of the IL-17 mRNA and RORγt mRNA by real time-PCR.Results Compared with the control group,the asthma models expressed more serious expression in bronchospasm contraction,hyperplasia disorders of bronchial epithelial cells,infiltration of inflammatory cells in lung,and so on.Compared with the control group,the total number of inflammatory cells counts[(104.04 ±5.51) 107/L vs (22.79 ± 1.91) 107/L] and eosinophils proportion [(37.63 ± 3.64) ％ vs (2.37 ± 1.55) ％] in BALF in group B were significantly increased (all P ＜ 0.05),the levels of IL-17 [(85.13 ± 5.77) 103 pg/L vs (47.44 ± 4.57) 103 pg/L] in BALF were significantly higher(P ＜ 0.05),the relative expressions of IL-17 mRNA (13.68 ± 1.59 vs 1.00 ± 0.00) and RORγt mRNA (4.53 ± 0.51 vs 1.00 ± 0.00) in lung were higher,which had a statistical significance (all P ＜ 0.05).The situations of group C,D,E were obviously improved compared with group B,and those of group D were improved remarkably.Conclusions Oral OM-85BV and 1,25-(OH)2-VitD3 intervention could relieve the airway inflammation of asthmatic mice models,and its effect can be remarkable by oral OM-85BV.The two immune regulators could relieve the degree of airway inflammation on asthmatic mice models by reducing the expression of Th17 cells differentiation.Therefore the two immune regulators could be the choices for preventing the happening and the development of the asthmatic airway inflammation.

Objective To evaluate quality of life in elderly patients treated with iodine-125 radioactive seed implantation for head and neck cancer.Methods From 2005 to 2011,40 elderly patients (≥ 65 years old)with head and neck cancer were treated with brachytherapy of 125I seed implantation alone (without radiation therapy history)and evaluated with QOL-RTI questionnaire for QOL.QOL of them were evaluated and relative factors were analyzed.Results QOL of these patients treated with 125 I-brachytherapy alone was satisfied.QOL in patients with the base tongue cancer was worse than that in others.Age had a significant effect on function/physical QOL and general QOL,but had no significant effect on emotional QOL and socioeconomic QOL.QOL of patients with early stage cancers were better than those with late stage cancers.Pathology,numbers of 125I seeds implanted,and time-period after brachytherapy had no significant effect on QOL.Conclusions QOL of elderly patients with head and neck cancer treated with125 I-BT is good.Tumor volume and clinical staging have significant effects on QOL.

The present article introduced the current medical education of the Feinberg School of Medicine in Northwestern University.Thmugh the comparisons on edueation system and carriculums design between United States of America and China,advices were made to the medical education in China.The article shed light on the current reform of medical education in China.

AIM\ To study the protective mechanism of ligustrazine(LT), shenmai injection(SI), combination of ligustrazine and shenmai injection (LSI) to injury of kidney after brain ischemia reperfusion in aged rats from the free radical change. METHODS Aged rats (more than 20 months) were divided into model group (Aged model), control group(Aged control), nimotop group(NG), ligustrazine group(LG), shenmai injection group(SIG) and LSI group(LSG). The following items was measured in aged rats with 60 min reperfusion after 30 min brain ischemia: the content of creatinine(Cr), urea nitrogen(BUN), MDA and the activitiy of superoxide dismutase(SOD). RESULTS The distinct pathological and functional injury in the kidney was found in the models, and this change was alleviated in each treatment group. The increase of MDA content in the models kidney tissue was obvious than that in the controls. The MDA level in the LG and the SIG was decreased. CONCLUSION The kidney injury after brain ischemia reperfusion in aged rats is correlated with the injury of free radical. LT, SI ,LSI and nimotop alleviate this injury. LT and SI restraine the injury of lipid peroxidation, this may be one of the mechanisms of restraining kidney injury after brain ischemia reperfusion.

AIM: To establish the quality control standard for Compound Dingfengzhitong Capsule (Radix Notoginseng, Radix Saposhnikoviae, Radix Angelicae Dahuricae). METHODS: Radix Saposhnikoviae, Radix Angelicae Dahuricae in capsule were identified by TLC, and the content of ginsenoside R g1 was determined by HPLC. RESULTS: Radix Saposhnikoviae, Radix Angelicae Dahuricae could be identified by TLC. The Ginsenoside R g1 showed a good linear relationship at a range of 0.5286?g～26.43?g, r=0.9999(n=6) , the average recovery was 99.94%, and RSD was 1.34%( n =9). CONCLUSION: The method is accurate with the good reproducibility and can be used for the quality control of Compound Dingfengzhitong Capsules.

Purpose To evaluate the value of imaging on the diagnosis and treatment of human-infected H10N8 virus. Materials and Methods The chest X-ray and CT features of one case of human infected H10N8 virus were retrospectively studied. Results A 73-year-old female patient was admitted to the third affiliated hospital of Nanchang University on November 30, 2013 due to cough, sputum for 3 days and fever for 1 day. The patient was diagnosed with severe pulmonary infection and underwent chest CT on December 1, which showed large opacities with air bronchograms were in the lower lobe of her right lung and ground-glass exudative lesions in the lower lobe of the left lung;the next 24 h, 48 h and 72 h review tracking chest X-ray showed the chest lesions developed rapidly which involved more lobes with more areas, integration of lesions and plenty of pleural effusion in a very short time. This was a typical white lung sign of acute respiratory distress syndrome. The patient died later on December 6, which was reported as the case of human-infected avian influenza (H10N8 virus) by Chinese Center for Disease Control and Prevention. Conclusion The chest imaging features found in human infected H10N8 virus present as progressive opacities and exudative lesions.

OBJECTIVE:To establish a method for simultaneous residual determination of dichloromethane and ethyl acetate in bisacodyl raw material. METHODS:Head-space GC was performed on the capillary column of 6% cyanopropyl phenyl-94% di-methyl polysiloxane(DB-624)by temperature programming,the temperature of injector was 220 ℃,detector was flame ionization detector with temperature of 250 ℃,carrier gas was high purity nitrogen with the flow rate of 3.0 ml/min,split ration was 1∶10, headspace heating temperature was 70 ℃,equilibration time was 30 min,volume of headspace vial was 5 ml,and the injection volume was 1 ml. RESULTS:The linear range was 6-120μg/ml for dichloromethane(r=0.999 9)and 50-1 000μg/ml for ethyl ac-etate(r=0.999 9);the limit of quantitation was 0.2,1.7 μg,limit of detection was 0.06,0.5 μg;RSDs of precision,stability and reproducibility tests were no higher than 3%;recoveries were 100.30%-102.00%(RSD=0.63%,n=9) and 100.10 %-101.30%(RSD=0.44%,n=9). CONCLUSIONS:The method is simple and accurate,and can be used for the simultaneous residual deter-mination of dichloromethane and ethyl acetate in bisacodyl raw material.