Objective To explore the relationship between FOLFOX Chemotherapy and GSTP1,XRCC1 gene polymorphism in patients with colorectal cancer.Methods 60 cases of colorectal cancer treated in Tumor hospital of Hunan Province from January to December 2014 were selected as the research object.Treat patients with modified FOLFOX regimen,determined the GSTP1 ,XRCC1 gene sequence polymorphism,and explored the relationship between the efficacy and the GSTP1 ,XRCC1 gene sequence polymorphism.Results The number of patients with complete remission and partial remission and the num-ber of patients with stable and progress were not related to the gender,age,tumor location,pathological differentiation,TNM staging of patients (χ2=0.222~1.8,P>0.05).Of all cases,the frequencies of GSTP1A/A,A/G and G/G genotype were 68.3%,23.3% and 8.3%,respectively.GSTP1 gene wild-type (A/A)patients were treated with less efficiency than the GSTP1 gene mutation type (A/G+G/G)patients (χ2=4.493,P=0.034).Of all cases,the frequencies of XRCC1 G/G,G/A and A/A genotype were 58.3%,36.7% and 5%,respectively.XRCC1 gene wild type (G/G)patients with effective rate was higher than that of mutant type (G/A+A/A)patients (χ2=4.691,P=0.030).Conclusion The study showed that GSTP1(A/A),XRCC1 (G/G)gene polymorphism may be associated with clinical response to FOLFOX chemotherapy in advanced colorectal cancer.

The urinary continence mechanism in the fetal and neonatal periods is the foundation of the development of continence function in the lower urinary tract (LUT).At present,it is rarely reported what is the fetus and newborns' LUT continence,and the domestic reviews about these knowledge is blank.The authors searched all the research reports about LUT continence and its mechanism in the fetal and neonatal period both in the human and animals,and reviewed the urinary continence of the fetus and newborns at first time at home,to fill the domestic blank of this field.

Objective To discuss clinical effect and clinical application value of lianqi capsules treated with the middle-late stage primary liver cancer. Method We choosed 168 cases of middle-late stage primary liver cancer patients in Guang'an District Hospital,Sichuan province,Guang'an treatment, and they were randomly divided into observation group and control group. Both two groups were treated with the same chemotherapy regimens. Pingxiaopian were auxiliarily used in control group, and lianqi capsules in observation group. Clinical curative effect and security of two groups were observed. Result There was no statistically significant difference between the total effective rate of clinical treatment group and control group. There were statistically significant differences between two groups on the quality of life period (P<0.05) and adverse reactions (P<0.05). Conclusion Lianqi capsules combined with chemotherapy was used in treatment of patients with locally advanced primary liver cancer, and it can effectively improve the patients’quality of life in the whole process, control the growth of the tumor, and increased synergistic effect of chemotherapy for patients. So Lianqi capsules was worthy in clinical application and popularization.

Objective To discuss the value of expressed prostate secretion(EPS) pH test in the diagnosis of chronie prostatitis. Methods EPS was obtained from 86 out-patients with chronic prostatitis, pH test and microscopy field being studied, prostate ultrasonography was performed after filling bladder. Results Mean pH of the EPS was (7.0±0.5), EPS-WBC was (20±10)/HP, and prostate ultrasonography positive rate were 63.9 % (55/86). pH of the EPS and the frequency of ultrasound abnormal ities were significantly correlated to the WBC in EPS.There was no correlation between age, disease process duration and the pH of EPS. Conclusion In chronic prostatitis, EPS-pH was positively correlated with the ultrasonography abnormalities and the WBC elevation of EPS, and EPS-pH test in the diagnosis of chronic prostatitis were meaning and value.

Sixty cases of acute lumbar sprain were treated by puncturing Yintang(Ex-HN 3) toward Shenting(GV 24), among which, 50 cases were cured, 9 were improved and only one failed, and the total effective rate was 98.3%.

Objective To investigate clinical significance and the variation of serum interleukin -6(IL-6) ,high-sensitivity C-reac-tive protein(hs-CRP) and T-cell CD40 ligand(CD40 L) in patients with rheumatoid arthritis (RA)(included activity group and stable group) .Methods 90 cases of RA patients in the observation group ,90 cases of healthy volunteers were selected as control group . Enhanced immunoturbidimetric assay was used in measurement of serum hs-CRP .Levels of CD40 L ,IL-6 were detected by enzyme-linked immunosorbent assay .Results BPC ,RF ,ESR ,hs-CRP ,IL-6 and CD40 L in peripheral serum of observation group were sig-nificantly higher than control group ,the differences were statistically significant (P<0 .05) .BPC ,ESR ,hs-CRP ,IL-6 and CD40 L in peripheral blood of patients in RA activity group were significantly higher than patients with RA in stable group ,the differences were statistically significant(P<0 .05) .RF ,ESR ,hs-CRP ,IL-6 and CD40L in peripheral serum of RA patients whose platelet ele-vated were significantly higher than RA patients whose platelet stay normal level ,the differences were statistically significant (t=6 . 070 ,3 .858 ,3 .272 ,3 .217 ,2 .253 ,P<0 .05) .Conclusion Serum hs-CRP ,IL-6 and CD40 L relate to RA inflammation closely .Com-bined detection can observe the condition changes and treatment effect in patients with RA .

Objective To construct the recombinant co-expression vector carrying antisense RNA to dual-target BAG-1 and Bcl-2 genes and recombinant single expression vector carrying antisense RNA to target BAG-1 and Bcl-2 gene respectively,then to pre-liminarily investigate their effect on the proliferation of gastric cancer cell SGC-7901 in order to lay the foundation for further study the effect of this recombinant vector on the tumor cells.Methods RT-PCR was used to amplify the full length of BAG-1 and Bcl-2 cDNA from total RNA of gastric cancer cell line SGC-7901.The BAG-1 cDNA fragment and the Bcl-2 cDNA fragment were insert-ed into pMD18-T simple vector respectively.The pMD18-T-BAG-1 was digested with BamH Ⅰ and Cla Ⅰ and the pMD18-T-Bcl-2 was digested with EcoR Ⅰ and Nhe Ⅰ.Then the BAG-1 cDNA fragment and the Bcl-2 cDNA fragment were inserted into the mcs1 and mcs2 of the eukaryotic co-expression vector pVITRO2 in the antisense orientation respectively.The construction of the single expression vector pVITRO2-AsBAG-1 and pVITRO2-AsBcl-2 was confirmed by restriction endonuclease treatment and se-quence identification.Then the Bcl-2 cDNA fragment was inserted into the mcs2 of the recombinant vector pVITRO2-AsBAG-1 in the antisense orientation to construct the co-expression vector pVITRO2-AsBAG-1-Bcl-2,and the recombinant vector was also iden-tified by restriction endonucleases digestion and sequence identification.Then the recombinant vector was transfected into SGC-7901 cell respectively.The proliferation of the cell was determined by the MTT assay.The level change of BAG-1 and Bcl-2 mRNA in SGC-7901 cell was detected by the semi quantitative RT-PCR and the change situation of cell cycle was detected by the flow cytom-etry(FCM).Results The restriction endonucleases digestion and sequencing identification indicated that the eukaryotic co-expres-sion vector pVITRO2-AsBAG-1-Bcl-2 and the single gene expression vector pVITRO2-AsBAG-1 and pVITRO2-AsBcl-2 were con-structed successfully.The MTT assay method demonstrated that compared with the control group,the recombinant vector could in-hibit the proliferation of the cells in time dependent manner,and the inhibiting effect was most notably in the 72 h transfection group(P <0.01);inhibition ratio of recombinant vector groups was significantly higher than that of control group and pVITRO2 group(P <0.01).BAG-1 and Bcl-2 mRNA expression in recombinant vector groups were significantly decreased compared with that in the control group and pVITRO2 group(P <0.01),The RT-PCR results showed that the expression level of BAG-1 mRNA and Bcl-2 mRNA was significantly decreased(P <0.01),moreover the co-expression vector group was more notably than the single expression vector groups(P <0.05),but the pVITRO2-AsBcl-2 had no obvious effect on the expression of BAG-1 mRNA(P >0. 05);the FCM detection results showed that the apoptosis rate of the recombinant vector groups was significantly higher than that of the control group and the pVITRO2 group with statistical difference(P <0.01),and the co-expression vector group was more nota-bly than single expression vector groups(P <0.01).In addition,the effect of the co-expression recombinant vector group was more significant than that of the single expression co-expression vector(P <0.01 ),the apoptosis rate was increased from 0.57% to 15.75%.Conclusion The co-expression recombinant vector pVITRO2-AsBAG-1-Bcl-2 and single expression vector pVITRO2-As-BAG-1 and pVITRO2-AsBcl-2 are successfully constructed and they can inhibit the proliferation of SGC-7901 cell and induce cell apoptosis,moreover the pVITRO2-AsBAG-1-Bcl-2 vector is most notably.

Objective To observe the effect of intravenous administration of dexamethasone on airway complications after laparoscopic cholecystectomy (LC). Methods 66 patients who were treated with laparoscopic cholecystectomy in Wenling hospital of traditional Chinese medicine were divided into the control group and the observation group, 33 cases in each group. The control group were injected with saline 2 mL and the observation group were received dexamethasone 10mg by intravenous infusion. The incidence of complications and complications were observed in the two groups at different times after treatment. Results At 1 hours, 4 hours, 12 hours and 24 hours after extubation, the differencesof the incidence of sore throat and throat pain degree between the two groups was not statistically significant. Conclusion Dexamethasone intravenous administration has no obvious effect on reducing airway complications after laparoscopic cholecystectomy.

Objective To study and analyze the effect of small dose dexamethasone on the prevention of throat pain at perioperative period of thyroid tumor resection. Methods 112 cases with thyroid cancer treated by resection in Wenling hospital of traditional Chinese medicine from July 2014 to July 2016 were selected as the study subjects which were divided into the study and the control group. The control group were given saline by intravenous injection, The study group were given small dose of dexamethasone intravenous injection. The preventive effect of postoperative throat pain and postoperative blood glucose level were compared between the two groups. Results Statistical analysis showed that the postoperative VAS score in the study group was better than that in the control group. The blood glucose level in the study group was better than that in the control group, the difference was statistically significant (P<0.05). Conclusion Perioperative treatment with small doses of dexamethasone is excellent for patients with thyroid tumors treated with resection. It can help patients prevent postoperative sore throat and has excellent application value and is worthy of clinical promotion.

Objective To observe the effect of supervised rehabilitation on functional recovery in hemiplegia patients after stroke.Methods 65 patients with acute stroke were divided into supervised rehabilitation group and no-supervised rehabilitation group. They were assessed with Ueda Test and Barthel Index before and after treatment. Results The scores of the Barthel Index in supervised rehabilitation group were significantly superior to those of the not supervised rehabilitation group (P <0.05), while the scores of Ueda Test between these two groups were not significantly different (P>0.05). Conclusion Supervised rehabilitation can improve the function recovery, especially in activities of daily living.