CD5+ B cells are derived from early stage precursors independently on bone marrow. It is an important subtype of B cells. Abnormalities of number and function of CD5+ B cells are found in several kinds of autoimmune diseases and B lymphocyte malignancies. Recently,the abnormalities are also found in AIDS,hepatitis, schizophrenia and the regeneration of liver cells, which indicates that CD5+ B cell may have extensively clinical significance.

Background:Psychological factor plays an important role in the pathogenesis of functional gastrointestinal disorders. Depression,anxiety may share the pathogenesis with irritable bowel syndrome( IBS),therefore,more and more attention has been paid to anti-depression and anti-anxiety in the treatment of IBS. Aims:To evaluate the effect of addition of flupentixol and melitracen tablets on IBS with depression and anxiety. Methods:Sixty IBS patients with depression and anxiety were randomly divided into control group and treatment group. Patients in control group were given live combined Bacillus subtilis and Enterococcus faecium enteric-coated capsules and trimebutine maleate,while paitents in treatment group were received flupentixol and melitracen tablets in addition to the above-mentioned medicine. Treatment course was 8 weeks. Hamilton depression scale,Hamilton anxiety scale and symptom score were evaluated before and after treatment. Results:Compared with control group,depression and anxiety in treatment group were obviously improved(P<0. 05);the relief rates of abdominal pain and diarrhea were significantly increased( P <0. 05 );and the total symptom score were significantly decreased(P<0. 05);the total effective rate was significantly increased(93. 3% vs. 76. 7%,P<0. 05). However,no significant difference in incidence of adverse reaction was found between the two groups. Conclusions:Additon of flupentixol and melitracen tablets can significantly improve the curative effect on IBS with depression and anxiety.

Objective To observe the effect of intravenous administration of dexamethasone on airway complications after laparoscopic cholecystectomy (LC). Methods 66 patients who were treated with laparoscopic cholecystectomy in Wenling hospital of traditional Chinese medicine were divided into the control group and the observation group, 33 cases in each group. The control group were injected with saline 2 mL and the observation group were received dexamethasone 10mg by intravenous infusion. The incidence of complications and complications were observed in the two groups at different times after treatment. Results At 1 hours, 4 hours, 12 hours and 24 hours after extubation, the differencesof the incidence of sore throat and throat pain degree between the two groups was not statistically significant. Conclusion Dexamethasone intravenous administration has no obvious effect on reducing airway complications after laparoscopic cholecystectomy.

Objective To study and analyze the effect of small dose dexamethasone on the prevention of throat pain at perioperative period of thyroid tumor resection. Methods 112 cases with thyroid cancer treated by resection in Wenling hospital of traditional Chinese medicine from July 2014 to July 2016 were selected as the study subjects which were divided into the study and the control group. The control group were given saline by intravenous injection, The study group were given small dose of dexamethasone intravenous injection. The preventive effect of postoperative throat pain and postoperative blood glucose level were compared between the two groups. Results Statistical analysis showed that the postoperative VAS score in the study group was better than that in the control group. The blood glucose level in the study group was better than that in the control group, the difference was statistically significant (P<0.05). Conclusion Perioperative treatment with small doses of dexamethasone is excellent for patients with thyroid tumors treated with resection. It can help patients prevent postoperative sore throat and has excellent application value and is worthy of clinical promotion.

The X protein (HBx) of Human hepatitis B virus (HBV) acts as an indirect transcriptional transactivator to regulate the expression of many viral and cellular genes, as well as playing a critical role in pathogenesis and the development of Hepatocellular carcinoma (HCC). Here we described the biological effects of HBx in association with four cellular factors, including inflammatory factors (COX-2 and iNOS), oncoprotein (Ras), and a newly identified tumor suppressor (YueF). The characteristics of these effectors, which might be associated with hepatocellular carcinoma, are also discussed.

Toxoplasmosis is an infectious disease which affects both human and animals.It distributes all over the world and makes great harm to human beings.In recent years,the morbidity of toxoplasmosis in our country increased because of contacting with pets.Toxoplasmosis has no specific clinical manifestations and responds well to correct treatment.In order to raise clinicians' notice to toxoplasmosis,this article will review the disease from the aspects of etiology,epidemiology,clinical manifestation and experimental diagnosis.

Cyclophilin A（CyPA） is a cytoplasmic protein which binds to immunosuppressive drug—cyclosporin A and possesses peptidyl-prolyl cis-trans isomerase activity. CyPA incorporated into virions through interactions with the Gag polyprotein in the life cycle of human immunodeficiency virus type 1(HIV-1) and its incorporation is essential for viral infectivity. This review discussed the function of CyPA in replication of HIV-1 virion and the usefulness of these studies for anti-HIV therapies.

Objective To establish the quantitative method for detecting perforin mRNA. Methods The competitive templates were prepared by restriction endonuclease method.The quantitative RT-PCR assay was established and used to detecting the perforin mRNA level in peripheral blood of tumor patients and healthy adults.Results Average perforin mRNA level of six healthy adults is 0.51±0.40 pmol/ml.The perforin mRNA level of patients with digestive tumors are significantly lower than that of healthy adults(P<0.01).But compared with healthy adults,no significant difference is showed in patients with breast cancer or leukemia.Conclusion The decreased perforin mRNA level probably attributes to the high incidence of cancers.Except for perforin-mediated cytolysis,additional effective mechanism against tumor cells maybe exist.

Dendritic cells(DCs) are known as the most efficient antigen-presenting cells that are potent stimulators of both B and T cell immune responses.The biological characteristics and culture in vitro of DCs,preparation of DC vaccines,experimental and clinical studies in tumor immunotherapy by DC vaccines are reviewed in this article.