Objective To explore the epidemic conditions of rheumatic fever (RF) with a simple method at some regions.Methods Antistreptococcal DNase B test microtiter method was adopted to detect human sera antibody in natural populations who are responsible to RF.All 216 schoolchildren aged 10～12 years old were chose by the random cluster sampling.The schoolchildren′s serum antibody against DNase B in each season from september 1988 to August 1989 was detected,and compared with antistreptolysin “O” (ASO);furthermore,5 different regional schoolchildren′s serum antibody to DNase B was tested, and 100 schoolchildren aged 10～13 years old were chose in every region.Meanwhile,the RF incidence of 5～18 year schoolchildren was investigated for 4 years,and approximately 364 915 person times were inquired.All the results of the anti DNase B and ASO were compared with the schoolchildren′s RF incidence.Results ① The levels of the anti DNase B were higher during fall,winter and spring seasons,and lower in summer,which were coincided with the RF incidence,and linear relation was very good ( r =0 913, P

Objective To investigate the protective effect of Schizandrae Lignanoid (SCL) on myocardial ischemia reperfusion injury in hyperlipidemic rats and its mechanisms and provide theoretical basis and experiment foundation for treatment of myocardial ischemia complicated with hyperlipidemic disease with SCL in the future. Methods After hyperlipidemic rat models were set up by oral administration of high lipid emulsion,the rats were divided into seven groups: control group,sham group,model group,SCL 60,20,5 mg?kg-1 groups and 200 mg?kg-1 CDT group.After reperfusion,the blood samples taken from the ventricles were assayed for blood lipid;MPO activities of ischemic myocardium were measured;Infarct area of left ventricles was measured by Evens blue-TTC staining,and myocardial pathological changes were also observed.Results Compared with model group, the myocardial infarct area/ventricle area ratios and MPO activities in SCL 60,20,5 mg?kg-1 groups decreased (P

Objective To investigate the protective effect of Schizandrae Lignanoid(SCL)on the apoptosis of PC12 cells induced by H2O2 and its relative mechanisms.Methods PC12 cells were divided into four groups: control group,model group,high dose SCL(SCL1) group,and low dose SCL(SCL2) group.Apoptosis of PC12 cells was induced by H2O2.The cell activity was determined by MTT,the cell apoptotic rate was determined by Annexin V-FITC/PI and ??m was detected by flow cytometry.The expressions of bcl-2 and bax were detected by immunohistochemistry.Results Compared with model group,SCL increased the survival rate of PC12 cells(P

Objective To evaluate clinical effects of using axonal repair technique (cell surgery) for the treatment of peripheral nerve injuries. Methods 12 patients with 13 injured peripheral nerves were treated with axonal repair technique of soaking the stumps of the nerve in a modified Collins fluid, freezing the stumps with liquid nitrogen, trimming the solidified stumps with a sharp blade, coapting the nerve with glue and two stitches of epineurium, so that the injured peripheral nerves were repaired at the axonal level. The postoperative evaluation was performed at an average of 9.7 months. Results In four of 12 cases operated upon with contralateral C 7 nerve root transfer(C 7 to ulnar nerve), regenerating axons had reached to the ipsilateral sternoclavicular joint or axilla at postoperative 4-7 months, so that the second stage of contralateral C7 nerve root transfer procedure could be performed. In five cases of which the accessory nerves were transferred to suprascapular nerves, the strength of supra-and infraspinatus of 3 patients had recovered to MRC 3-4 at postoperative 16 months, and that of the other two, to MRC 1-2 at 12 months. In one case undergoing the second stage procedure of contralateral C 7 nerve root transfer (ulnar nerve to median nerve), the flexor carpi radialis showed some nerve regeneration shown in EMG at postoperative 4 months. In one case of which phrenic nerve was transferred to the musculocutaneous nerve (the nerve graft 8 cm), the biceps showed some nerves regeneration shown in the EMG 15 months postoperatively. In one patient for whom two digital nerves were repaired, the protective sensation had recovered 3 months postoperatively. Conclusion Axonal repair technique is an effective and practical way for the treatment of peripheral nerve injury.

At the concentrations of 10-8mol/L, 5.5?l0-8mol/L, 10-7mol/L morphine produced a dose-dependent increase of beating rate in cultured rat heart cells with 9%,32%and 81% respectively. The increase was antagonized by opiate antagonist naloxone ( 10-5mol/L ) , but not affected by propranolol ( 10-5mol/L ) which could cancel the positive chronotropy of isoprenaline ( 10-9mol/L ) on the beating cells. The results support the concept that morphine may be a direct effect on myocadium, perhaps through opiate receptors.

Objective To investigate the clinical effect and security of spraying Zhikang Capsules guided by gastroscopy in treatment elderly upper gastrointestinal bleeding.Methods Eighty-four elderly gastric duodenal ulcer bleeding patients were randomly assigned to group A,B and C (n =28 for each group).Patients in group A were used noradrenalin to spray with gastroscopy,in group B were used thrombin and in group C were used Zhikang Capsules.The total effective,the immediate homeostasis rate,the incidence of rebleeding,the emergency operation rate and the incidence of adverse reaction were recorded and analyzed.Results Total effective rate of group C was 96.4％ (27/28),significantly higher than that in group A (71.4％,20/28),B (75.0％,21/28) and the difference was significant(P＜0.05).The incidence of rebleeding in group A,B,C were 28.6％ (8/28),17.9％ (6/28),3.6％ (1/28) respectively,and the difference was significant (P ＜0.05).The same trend was seen regarding of the incidence of adverse reaction among three groups (25.0％,7/28;10.7％,3/28;0,0/28;P＜0.05).Conclusion Spraying Zhikang Capsules guided by gastroscopy is showed with good therapeutic effect in treating elderly upper gastrointestinal bleeding and high safety,and with less adverse reaction.

Objective To study the changes of corneal sensitivity in diabetic and nondiabetic subjects after phacoemulsification. Methods This trial involved 42 diabetic subjects(42eyes)and 46 nondiabetic subjects(46 eyes)with cataract.All eyes were underwent phacoemulsification.Corneal sensitivities were tested before surgery and 1 day,1 week,1 month,3 month,6 month after surgery.Results The corneal sensitivity of diabetic subjects was greatly reduced at 1 day,1 week,1 month,3 month after surgery(P＜0.05),and returned to the preoperative values at 6 month(P＞0.05).The corneal sensitivity of nondiabetic subjects was reduced at 1 day,1 week,1 month af- ter surgery(P＜0.05),and returned to the preoperative values at 3 month(P＞0.05).The corneal sensitivity of diabetic subjects was lower than nondiabetic subjects before surgery(P＜0.05).The reduced range of corneal sensitivity in diabetic subjects was greater than that of nondiabetic subjects(P＜0.05).Conclusions The corneal sensitivity is decreased and the recovery is postponed after poacoemul- sification in diabetic subjects.

ObjectiveTo détect in the tissue of primary bronchogenic carcinoma(PBC) and discuss its significance. Methods was assessed by immunohistochemical method ot SP in paraffin tissue sections of 40 PBC and paratumor normal lung tissue. Results The positive rate of TNF-a in cytoplasm of carcinoma cells was 47.5 % (19/40), and it was not found in all paratumor normal lung tissue(P< 0.05). ConclusionTNF-a gene expressed in some patients with broncho genic carcinoma. The growth of bronchogenic carcinoma is related to TNF-a gene.

Objective The DNA fingerprints of cultivated Pinellia ternata collected from different geographic regions were generated by using AFLP markers to find the feasibility in analyzing their genetic diversity, relationship, and germplasm identification. Methods The DNA polymorphism of 51 cultivated germplasm of P. ternata collected from 17 different habitats and four cultivars of P. pedatisecta (outgroup) were detected by AFLP molecular markers. Results The DNA fingerprints of 51 individuals of P. ternata were obviously distinguished by eight pairs of high polymorphic and efficient primer combinations screened from 64 primer combinations. The phylogenetic clustering results revealed that all the tested cultivars were fully differentiated, and individuals from the same regions were mainly clustered together. Moreover, cultivars from East-China, including Zhejiang and Jiangsu Provinces, displayed clear genetic distinction from other regions. The clustering results were strongly supported by Bootstrap test. Conclusion AFLP Markers can be potentially used in analyzing of genetic diversity, relationship, and germplasm identification of this medicinal plant, and the germplasm from regions of East-China, including Zhejiang and Jiangsu Provinces, displays the relative separate genetic characters from other regions.

Objective To study DSA characteristics of traumatic renal hemorrhage and to discuss the methods for treatment of this disease.Methods Superselective renal arterial angiography was carried in 14 patients with traumatic renal hematuria from 2001~2007.11 cases were iatrogenic renal damage and 3 cases were renal trauma.6 of them were under unstable hemodynamic conditions.Results The bleeding was found in 7 cases,located at the interlobar artery,the branch of segmental artery of the kidney,and the proximal end of arcuate artery.They presented as renal arterial-pelvic fistula(4 cases),arterial venous fistula(1 case) and renal arterial-pelvic fistula with AV fistula(2 cases),2 of them accompanied with pseudoaneurysm.The characteristics on DSA were radiopaque contrast medium overflow rapidly and abundantly,and the lesions were far from peripheral area of kidney,in the other 7 patients,the bleeing arteriae were located at the distal end of arcuate artery and the smaller branches of interlobular artery.The radiopaque material remained in parenchyma of kidney until parenchymal phase.There were no obvious artery-vein fistula or renal arterial-pelvic fistula in all cases.Conclusion DSA examination is very helpful in diagnosis and treatment of traumatic renal hemorrhage.