BACKGROUND:Though the high-resolution CT(HRCT)could identify the inner structures of temporal bone.its tiny parts could hardly be observed accurately and clearly by the imaging examinations of auriculotemporal portion,with the influence of scanning layers as well as the partial volume phenomenon.However,it could be easily identified by the combination of thin sections and HRCT images.OBJECTIVE:To introduce a method which could locate the posterior tympanum and its neighboring structures using HRCT images and to provide an anatomical base for the imaging diagnosis and operative treatment on this area.DESIGN,TIME AND SETTING:The observations between the auriculotemporal sections and CT images were finished both in thc Department of Sectional and Imaging Anatomy,Medical College,Nanchang University and the Center of Medical lmageology.Jiangxi Provincial People's Hospital from July 2004 to June 2007.MATERIALS:Fifteen normal adult cadaveric heads(30 sides)which fixed by 100 g/L formaldehyde were scanned,with all the samples provided by the Department of Anatomy,Medical College,Nanchang University.The main equipment was GE Hi-speed Nx/i Sys CT equipment (GE Company,USA).METHODS:Tb obtain CT images of temporal bone(depth 1.00 mm,thickness 1.00 mm),15 normal adult cadaver heads were scanned by CT method taking callthomeatal line(CML)as the baseline.After that,specimens of auriculotemporal portion from temporal bone were taken,decalcified,desiccated and embedded.Sequential sections(thick 1.00 mm)were made.MAIN OUTCOME MEASURES:Comparing sequential sections with CT image,identified respectively the fossa incudis,chorda eminence,styloid eminence,tacial recess,sinus tympanl,ponticulus promontoni,suprameatal spine,etc.RESULTS:The depth of fossa incudas was about(1.49±0.05)mm,the distance from the fossa to pyramid segment of facial nerve was(5.67±0.1 4)mm.The distance from the medial wall of posterior tympanic sinus to the horizontal segment of facial nerve was(3.1 2±0.1 5)mm.The average distance from suprameatal spine to the vertical segment of facial nerve was (16.73±1.24)mm,to chorda tympani nerve(15.87±1.14)mm,to promontory(21.84±2.43)mm.CONCLUSION:Comparing the sectional antomy and CT image of auriculotemporal potion of temporal bone is valuable for the diagnosis and treatment of otopathy.

Objective To compare imaging features and to evaluate clinical values of aortic dissection(AD) with MRI and spiral CT.Methods MRI and spiral CT findings of AD in 18 cases confirmed by clinical or surgical pathology were analysed retrospectively.Results The sensitivity and specificity of two advanced techniques in diagnosis of AD were high.MRI and CT showed the dimension,true or false lumen,intimal flap,intimal tear,thrombus and intramural hematoma of AD,aortic dilatation or stenosis and the major aortic branches.Conclusion The two techniques are important for the suspect AD,two techniques are similar to display the features of dimension,classification,true or false lumen and aortic dilatation or stenosis,MRI plays an important role in diagnosis of AD and is better than CT in demonstrating the intimal flap,intimal tear,thrombus,intramural hematoma and the involvement of the major aortic branches,there were limitation in demonstrating calcification of intimal flap or vascular wall and emergency patients.

Objective: This paper investigates Shenmai Injection’s effect on the cellular immunity of patients with acute exacerbated chronic obstructive pulmonary disease (AECOPD). Methods: 92 cases of AECOPD patients are randomly divided into two groups----control group and treatment group, each with 46 patients. Patients in the two groups are given the same treatment such as anti-inflammatory, astiasthmatic, eliminating phlegm, inhale oxygen. Based on conventional treatment, the treatment group dissolves 40mL of Shenmai Injection in 250mL of injection glucose (5%). Such solution is dripped in vein continuously for two courses of treatment, with ten drops for a course of treatment and once a drop. Indexes of CD+3, CD+4, CD+8of peripheral blood T lymphocyte subsets and ratio of CD+4/CD+8 are tested before two weeks and after two weeks of the treatment, in order to discuss the effect of Shenmai Injection on the cellular immunity of patients with acute exacerbation of chronic obstructive pulmonary disease. Results: After two weeks' treatment, the indexes of CD+3, CD+4 raise up, the indexes of CD+8 go down, and the ratio of CD4+/CD8+ increases. In contrast, there is a significant difference for the patients before the treatment; So is the same with Indexes of CD+3, CD4+, CD8+ and ratio of CD+4/CD+8 to the patients in the treatment group and those in the control group after two weeks. Conclusion: Shenmai Injection can improve the AECOPD patient’s cellular immunity.

OBJECTIVE To assess the effect of Ibudilast on the quality of life (QOL) of patients with steroid resistant allergic rhinitis.METHODS A randomized,double-blind,placebo-controlled study was carried out with Ibudilast in 66 patients with the steroid resistant allergic rhinitis.QOL was measured with Short-Form Health Survey (SF-36) questionnaire (Chinese version).Ibudilast (10rag twice daily,34 patients),placebo (32 patients) was given for 3 weeks. The SF-36 questionnaire was administered at the start of treatment and after 3 weeks of treatment.Sings and Symptom-medication scores were measured daily during the study.RESULTS There were no significant differences between the Ibudilast and placebo group in terms of sings and symptoms or QOL scores before study.After 3 weeks of treatment,symptoms scores were significantly decreased and QOL scores significantly improved in the Ibudilast group compared with those in placebo group (both P

Objective To investigate the differences of measurement of gross target volume (GTV)between endoscopic ultrasonography ( EUS )-based ( GTVEUS ) and computed tomography ( CT ) -based (GTVCT) method for thoracic esophageal squamous cell carcinoma. Methods EUS was performed on 36consecutive patients with thoracic squamous cell carcinoma, and the superior and inferior boarders of the tumor defined by EUS were marked with hemoclips. The CT planning scan was then performed with the patient in supine position, and the GTVCT and GTVEUS were contoured respectively. The lengths ( LCT and LEUS) and spatial locations of longitudinal GTVCT and GTVEUS were compared. Results The mean LCT and LEUS were (7. 79 ± 3. 15 ) cm and (7. 42 ± 2. 72) cm, respectively ( t = 0. 82, P ＞ 0. 05 ), with a correlation coefficient of 0. 61 (P ＜0. 001 ). Locations of longitudinal GTVCT and GTVEUS were compared in 34cases, with 2 excluded for invisualization on CT. The mean conformal index (CI) was (0. 79 ± 0. 18 ), and spatial variations were found in 71% patients, with 8 patients at proximal end and 21 others at distal end.There was no clip placement associated complication. Conclusion Endoscopic hemoclips placement is safe and reliable. EUS can provide additional information to CT in defining longitudinal GTV in thoracic esophageal squamous cell carcinoma, especially in superficial and submucosal carcinomas.

Objective: To analyze the prevalence and associated factors of suicide ideation among adolescent students in Guizhou Province, and to provide scientific evidence for developing strategy. Methods: Multistage stratified cluster sampling method was used to recruit 4 903 adolescents from 14 middle schools in Guizhou Province. China Youth Health Risk Behaviors Questionnaire was used and data were analyzed by using univariate analysis and Logistic regression analysis. Results: Totally 14.3%(703/4 903) middle school students had suicide ideation during the past 12month period, in which 13.2% were boys and 15.4% were girls, more girls were prone to suicide than boys(χ2=5.20, P<0.05); 13.7% junior students, 15.0% senior students, 14.5% Han ethnic students and 14.1% minority students suicide ideation, and the differences between grades and ethnic groups were of no statistical significance (P>0.05). The multivariates Logistic regression analysis showed that risk factors for suicidal ideation included being girl(OR=1.49),higher maternal education(college and above: OR=2.26), suffering psychological bullying over the past 30 days(OR=1.30), taking part in fighting for the past 12 months (OR=1.33), often feeling lonely(OR=1.86), sleeplessness(OR=1.38), deliberate selfinjury(OR=3.58),decreases of daily activities due to depression(OR=1.87), ideation of running away(OR=3.31), internet addiction(OR=1.37), walk violating regulations(OR=2.42), but occasional low mood from academic pressure(OR=0.73) was a protective factor for suicidal ideation(P<0.05). Conclusion Suicide ideation of middle school students is closely related to campus violence, adverse inner metal experience and unhealthy behavior pattern. All sectors of society should attach great importance and targeted measures to prevent suicide and protect the students.

OBJECTIVETo amplify mouse prostate stem cell antigen (mPSCA) gene and construct a recombinant plasmid to obtain mPSCA protein and identify its antigenicity.

METHODSThe gene of mPSCA was amplified by RT-PCR from mouse prostate cancer cell line RM-1 with the signal peptide sequence removed. The PCR product was cloned into pET-42a prokaryotic expression vector to construct the recombinant plasmid pET-42a-mPSCA, which was transformed into BL21 (DE3) for mPSCA expression. The fusion protein was purified and identified by SDS-PAGE and Western blotting. The antigenicity of the purified protein was characterized by ELISA.

RESULTSThe mPSCA gene was obtained with an identical sequence to that retrieved in GenBank. The prokaryotic expression vector for mPSCA was successfully constructed as confirmed by enzyme digestion and DNA sequencing. Both Western blotting and ELISA demonstrated the antigenicity of the purified mPSCA protein.

CONCLUSIONThe purified mPSCA obtained possesses good antigenicity, which will facilitate further study of immunotherapy for prostate cancer targeting PSCA.

Animals ; Antigens, Neoplasm ; genetics ; immunology ; isolation & purification ; Cloning, Molecular ; Escherichia coli ; metabolism ; GPI-Linked Proteins ; genetics ; immunology ; isolation & purification ; Genetic Vectors ; Male ; Mice ; Neoplasm Proteins ; genetics ; immunology ; isolation & purification ; Plasmids ; Prostate ; cytology

Objective: To explore the characteristics of psychological control of parents of college students and their correlation with health risk behaviors, and to provide a reference for further improving parenting styles and improving the physical and mental health of college students. Methods: With the method of multi-stage stratified random sampling, 3 318 college students from 6 universities in Guizhou Province were selected. The parents’ psychological control questionnaire and Chinese adolescent health-related behavior questionnaire were used to conduct anonymous field survey. Results: A total of 660 students’ parents have got low score of mental control in low-score group (<26), 1 844 in the middle group (26-49) and 814 in the high group (>49). Students who had suicide plans scored higher in the dimensions of invasion of privacy, inhibition of speech expression, and sense of disability than those who had not(t=4.08, 3.74, 3.82, P<0.01); The scores of smokers in achievement expectation, invasion of privacy and ability ineffectiveness were higher than those of non-smokers(t=7.12, 3.91, 8.10, P<0.01); the scores of drinkers in all dimensions were higher than those of non drinkers(t=9.35, 4.64, 5.90, 8.78, P<0.01); the scores of Internet addicts in all dimensions were higher than those of non-smokers(t=8.92, 14.27, 3.70, 15.94, P<0.01). Spearman correlation analysis showed that smoking, drinking and Internet addiction were positively correlated with parents’ psychological control(r=0.11, 0.15, 0.19, P<0.01). Conclusion Parents’ psychological control of college students in achievement expectation, ability ineffectiveness and speech inhibition; parents’ psychological control is positively related to smoking, drinking and Internet addiction behavior. Parents should give more active encouragement and guidance to their children and strengthen communication.

Objective: To understand the sanitation status of rural primary and secondary schools during 2015-2019, and provide data support for the formulation of environmental sanitation policies for schools in rural areas of Guiyang. Methods: According to the requirements of the Work Plan for the Rural Environmental Sanitation Monitoring Project in Guiyang City from 2015-2019, 145 rural schools in Guiyang were selected by stratified random sampling method, including 75 elementary schools and 70 junior high schools. Through on site inspection and questionnaire survey, information regarding school basic situation, drinking water for students, water supply and toilet sanitation in school. Results: Students drinking water was mainly bottled water or water from a water purifier, the proportional rate increased from 37.9% to 72.4%; water supply in the school was mainly from village water plant, from 69.0% to 93.1%; the proportion of schools with independent public toilets and toilets in dormitory buildings ranged from 65.6% to 100.0%, the proportion of schools with toilets in teaching buildings was less than 60.0%, but the penetration rate of sanitary toilets in independent public toilets, teaching buildings and dormitory buildings were more than 80.0%. Qualified rate of squatting in the men s toilet fluctuated between 93.1% and 100.0%. Qualified rate of the squatting in the women s toilet had increased from 44.8% to 65.5%. Proportion of school toilets guarded by specific staff decreased from 31.0% to 27.6%. The proportion of soap increased from 20.7% to 58.6%, the difference was statistically significant ( χ 2=21.27, P =0.01). Conclusion During 2015-2019, the environmental sanitation in rural areas of Guiyang was acceptable, with students drinking water, school water supply and sanitary toilets moderately improved. Toilets in the teaching building and women s toilet squatting numbers need to be increased. School toilets managed by specific personnel, as well as good hygiene habits education promotion are encouraged.

Objective: To understand the smoking of college students in Guizhou Province and to explore associated factors for better prevention and control of smoking behavior. Methods: A multi-stage stratified random sampling method was used to describe the current smoking situation of college students. And 2 451 college students of different majors and grades from three cities with high, medium and low economic development levels were selected. Besides, we used multivariate logistic regression analysis to analyze the major factors affecting current smoking. Results: Ever smoking rate of college students in Guizhou Province was 50.8%, the peak age of first smoking attempt was 8-13(35.2%); current smoking rate of college students in Guizhou Province was 20.8%. Logistic regression analysis showed that male, upper grade, monthly living expenses of not less than 1 000 yuan, single parents, family smoking and peer smoking were the main risk factors affecting college students’ smoking(OR=5.52,1.44,1.48,1.69,1.47,1.88,P<0.05). Conclusion College students in Guizhou Province have a higher rate of smoking attempt and low rate of current smoking, as well as low tobacco dependence which is determined by multiple factors. Tobacco hazard education, mental health education and living environment intervention for college students may help reduce the probabitity for tobacco use.