Objective To develop a method of determining benzene hydrocarbon and halogenated alkane hydrocarbon in the air of workplaces with the capillary gas chromatography of carbon disulphide desorption. Methods Benzene hydrocarbon and halogenated alkane hydrocarbon in the air of workplace were collected by active carbon sampling cuvette, then separated by hydrogen flames detector gas chromatography machine after carbon disulphide desorption. Benzene hydrocarbon and halogenated alkane hydrocarbon were determined quantitively by retention time and quantitatively by apex area. Results The linear ranges of benzene, toluene, p-xylene, m-xylene, o-xylene, ethyl benzene, styenl, chlorobenzene, acetone, carbontetrachloride, dichloromethanl, trichloromethane, 1, 2-dichloroethane, naphth alene were 0.019-81.600, 0.018-91.200, 0.018-88.800, 0.018-56.8, 0.011-92.000, 0.012-63.200, 0.018-93.200, 0.449-2298.400, 0.252-1287.000 and 0.076-390.000 mg/m3 respectively. The recovery rates were 88.4%-98.6% and RSD were 1.0%-6.0%. Conclusion This method can separate efficiently and determine accurately benzene hydrocarbon and halogenated alkane hydrocarbon in the air with a good precision. It is suitable for the determination of the toxicants in the air.

Objective To establish a method for determining VOCs in drinking water by headspace gas chromatography. Methods VOCs in the water were extracted by headspace technology, then analyzed with Rtx-WAX capillary column, in the same time, VOCs was determined with GC by controlling the temperature and the speed of nitrogen. The retention time of the peaks was used for qualitative analysis, external standard method was used for quantitative analysis. Results The linear ranges of dichloromethane, benzene, toluene, 1, 2-dichloroethane, ethylbenzene, p-xylene, m-xylene, o-xylene, isopropylbenzene, chlorobenzene, styrene were 6.2-311.2, 2.0-100.0, 1.6-81.5, 5.7-282.8, 1.9-93.4, 1.7～85.4, 1.7-87.2, 2.1-103.2, 1.5-76.3, 2.2-107.3 and 1.9-96.0 ?g/L respectively. The lowest determination limit were 0.5-5.9 ?g/L, the rate of recovery were 88.8%-109.6% and RSDs were 2.2%-5.9%. Conclusion This method is simple, rapid, sensitive and can efficiently separate and accurately determine 11 kinds of VOCs in drinking water.

Objective To establish a method for simultaneous determination of kelthane and pyrethroid pesticide residues in water.Methods Kelthane and pyrethroid pesticide residues in the water were extracted by liquid-liquid,then analyzed with DB-1 capillary column.In the same time,they were determined with GC by controlling the temperature.Retention time of the peaks was used for qualitative analysis,while external standard method was used for quantitative analysis.Results The linear ranges of Kelthane,Bifenthrin,Fenpropanate,Lambda cyhalothrin,Permethrin,Beta cyfluthrin,Alphacypermethrin,Fenvalerate were 0.24-31.1,0.21-27.2,0.20-26.0,0.21-26.5,0.20-25.7,0.21-26.4,0.22-29.0 and 0.22-27.4 ?g/L,r≥0.999 0.The lowest determination limits were 0.20,0.33,0.30,0.13,0.36,0.26,0.33 and 0.30 ?g/L,the rate of recovery was 86.0%-111.7% and RSD was 3.8%-11.8%.Conclusion This method can separate efficiently and determine 8 kinds of pyrethroid pesticide residues in the water and only needs 23 minutes.It's simple,rapid and sensitive.

Objective To establish a test method of dichloromethane,1,1-dichloroethylene,1,2-dichloroethylene,1,2-dichloroethane in drinking water with headspace gas chromatography.Methods Halogenated alkane hydrocarbon in the water was extracted by headspace technique,then analyzed with DB-624 capillary column.In the same time,determined with GC by controlling the temperature.Retention time of the peaks was used for qualitative analysis,while external standard method was used for quantitative analysis.Results The linear ranges for dichloromethane,1,1-dichloroethylene,1,2-dichloroethylene,trans-1,2-dichloroethylene,1,2-dichloroethane were 0.85-168,0.07-12.1,0.40-77.8,0.53-119 and 1.2-265 ?g/L respectively,the determination limits were 0.83,0.07,0.40,0.53 and 1.10?g/L respectively,r≥0.999 5,the rate of recovery were 102.5%-113.8%,and RSDs were 5.5%-11.8%.Conclusion This method is simple,rapid and sensitive,can efficiently separate and accurately determine 5 kinds of halogenated alkane hydrocarbon in the water and only takes 5.2 minutes.

Objective To establish the method of acetaldehyde and acraldehyde in water with headspace gas chromatography. Methods Acetaldehyde and acraldehyde in the water samples were extracted by headspace technology,then analyzed with DB-624 capillary column. In the same time,they were determined with GC by controlling the temperature. Retention time of the peaks was used for qualitative analysis,while external standard method was used for quantitative analysis. Results In 3.0-250 ?g/L, the regression equation for acetaldehyde was y=406.83 x+0.847,r=0.999 9,the lowest detection limit was 1.0 ?g/L. In 6.2-500 ?g/L, the regression equation for acraldehyde was y=207.53 x-0.450,r=0.999 8,the lowest detection limit was 3.3 ?g/L. The rates of recovery were 90.0%-95.5%,and RSDs were 2.1%-3.7%. Conclusion This method is simple,rapid,sensitive and is applicable to the determination of acetaldehyde and acraldehyde in water.

BACKGROUND:Though the high-resolution CT(HRCT)could identify the inner structures of temporal bone.its tiny parts could hardly be observed accurately and clearly by the imaging examinations of auriculotemporal portion,with the influence of scanning layers as well as the partial volume phenomenon.However,it could be easily identified by the combination of thin sections and HRCT images.OBJECTIVE:To introduce a method which could locate the posterior tympanum and its neighboring structures using HRCT images and to provide an anatomical base for the imaging diagnosis and operative treatment on this area.DESIGN,TIME AND SETTING:The observations between the auriculotemporal sections and CT images were finished both in thc Department of Sectional and Imaging Anatomy,Medical College,Nanchang University and the Center of Medical lmageology.Jiangxi Provincial People's Hospital from July 2004 to June 2007.MATERIALS:Fifteen normal adult cadaveric heads(30 sides)which fixed by 100 g/L formaldehyde were scanned,with all the samples provided by the Department of Anatomy,Medical College,Nanchang University.The main equipment was GE Hi-speed Nx/i Sys CT equipment (GE Company,USA).METHODS:Tb obtain CT images of temporal bone(depth 1.00 mm,thickness 1.00 mm),15 normal adult cadaver heads were scanned by CT method taking callthomeatal line(CML)as the baseline.After that,specimens of auriculotemporal portion from temporal bone were taken,decalcified,desiccated and embedded.Sequential sections(thick 1.00 mm)were made.MAIN OUTCOME MEASURES:Comparing sequential sections with CT image,identified respectively the fossa incudis,chorda eminence,styloid eminence,tacial recess,sinus tympanl,ponticulus promontoni,suprameatal spine,etc.RESULTS:The depth of fossa incudas was about(1.49±0.05)mm,the distance from the fossa to pyramid segment of facial nerve was(5.67±0.1 4)mm.The distance from the medial wall of posterior tympanic sinus to the horizontal segment of facial nerve was(3.1 2±0.1 5)mm.The average distance from suprameatal spine to the vertical segment of facial nerve was (16.73±1.24)mm,to chorda tympani nerve(15.87±1.14)mm,to promontory(21.84±2.43)mm.CONCLUSION:Comparing the sectional antomy and CT image of auriculotemporal potion of temporal bone is valuable for the diagnosis and treatment of otopathy.

Objective To develop an HPLC method for determination of triptoquinone H in Tripterygium wilfordii and its Tablet preparations. Methods An external method with Agilent Zorbax SC-C_(8)(250 mm?4.6 mm, 5 ?m) column as fixed phase and methanol-water (75∶25) as mobile phase was adopted. The detective wavelength was 258 nm and the flow rate was 1.0 mL/min. Results The linearity range of triptoquinone was 6.28 — 100.5 ?g/mL (r=0.999 7). The average recovery of T. wilfordii was 96.94%, RSD was 1.57% (n=9) and the average recovery of T. hypoglaucum Tablet was 100.02%, RSD was 1.74% (n=9). Conclusion The method is accurate and sensitive. It is adoptable for quantity analysis of triptoquinone H in T. wilfordii and its Tablet.

Objective To determine the content of tripterine in Tripterygium wilfordii and its tablets by HPLC.Methods An external standard method by HPLC with Zorbax C_(18)column as fixed phase and methanol-1% HAc(87∶13) as mobile phase was adopted.The detection wavelength was 425 nm and the flow rate was 1.0 mL/min.Results The linear range for tripterine was 40.96～204.8 ?g/mL(r=(0.999 6).) The average recovery of Chinese medicinal materials was 98.37% and RSD was 1.01%(n=9);the average recovery of preparation sample was 98.59% and RSD was 1.18%(n=9).Conclusion The method is simple and accurate,which can be adoptable for quantitative analysis of tripterine in the plants of Tripterygium L.

Objective To establish an HPLC method for determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii(RFFTW) and its tablets.Methods An external method with HiQ siL KYA-C_(18)(250 mm?4.6 mm,5 ?m) column as fixed phase and methanol-1% acetic acid solution((66∶)34) as mobile phase was adopted.The detective wave length was 254 nm and the flow rate was 1.0 mL/min.Results The linearity range was 6.62—105.9 ?g/mL(r=0.999 9),the average recovery of T.wilfordii from Hunan Province was 97.78%,RSD was 1.09%(n=9),and the average recovery of tablet of T.hypoglaucum was 99.83%,RSD was 1.76%(n=9).Conclusion The method is accurate and sensitive.It is adoptable for quantitative analysis of triptoquinone B in RFFTW and its tablets.

In order to prove that ectopic over-expression of Pim-2 could induce malignant transformation of human liver cell line L02, three groups of cells were set up including human liver cell line L02 (L02), L02 cells transfected with Pim-2 gene (L02/Pim-2) and L02 cells transfected with empty-vector (L02/Vector). Pim-2 expression levels were detected. The morphology, proliferation level, apoptosis rate and migration ability of the cells were detected respectively. Then the cells were subcutaneously inoculated into athymic mice and the microstructures of the neoplasm were observed. Compared with the controls, Pim-2 expression levels were significantly higher in L02/Pim-2 cells (P<0.05), and their morphology had obvious malignant changes. They also showed a significantly increased proliferation rate (P<0.05) and migration capacity (P<0.05), as well as a significantly decreased apoptosis rate (P<0.05). Only the athymic mice inoculated with L02/Pim-2 cells could generate neoplasm, and the morphology of the neoplasm coincided with that of the hepatoma. The results manifest that ectopic Pim-2 gene could be stably expressed in L02/Pim-2 cells. Both the morphological and biological changes of L02/Pim-2 cells demonstrate the trend of malignant transformation. L02/Pim-2 cells could generate hepatoma in athymic mice. In conclusion, Pim-2 could induce malignant transformation of human liver cell line L02.
Animals ; Apoptosis ; Cell Line ; Cell Movement ; Cell Proliferation ; *Cell Transformation, Neoplastic ; Humans ; Liver/pathology/physiology ; *Liver Neoplasms/genetics/pathology ; Mice ; Mice, Nude ; Neoplasm Transplantation ; *Oncogenes ; Protein-Serine-Threonine Kinases/genetics/*metabolism ; Proto-Oncogene Proteins/genetics/*metabolism