1.Changes of the levels of tumor necrosis factor and interleukin-8 in the blood and gastric mucosa and their mRNA expression after liver ischemia- reperfusion
Yankui CHU ; Qingjiu MA ; Wei LIU ; Jiangou LU ; Xilin DU ; Deming GAO ; Shengzhi WANG ; Tao WU
Chinese Journal of General Surgery 1997;0(06):-
Objective To investigate the changes of the levels of TNF and IL 8 in blood and in gastric mucosa and their mRNA expression after liver ischemia reperfusion (I/R). Methods 130 rats were randomly divided into 3 groups: (1) sham operation group(control group, n=10);(2)liver ischemia reperfusion group (n=60), including ischemia for 20min and 40min, and reperfusion for 1h, 24h and 72h respectively; (3) liver I/R rats treated with famotidine group (n=60). ALT and AST were detected after operation, pathomorphological changes of gastric mucosa were studied by light microscope and transmission electronic microscope, the concentrations of TNF and IL 8 in blood and gastric mucosa were measured by radioimmunoassay (RIA), TNF mRNA and IL 8 mRNA were detected by in situ hybridization. Results After liver I/R,inflammatory damage appeared in gastric mucosa, TNF and IL 8 concertrations increased significantly(P
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