Intraaterial injection of superoxide dismutase (SOD)15000U/kg 15 min before releasing the obstruction is given . 35 rats are divided into 3 groups : group 1, 7 for control; group 2,14 for releasing the obstruction for 1 h ; in which (7 as 2A , 7 as 2B ) 2B are given SOD ; group 3 , 14 for releasing the obstruction for 2 h , in which (7 as 3a , 7 as 3B ) 3B given SOD . The results show : the content of malondialdehyde (MDA) in bowel , and heart tissue in 2A increases 66% and 55% (p 05) vis group 1 ; contents of MDA in bowel, heart, liver , and lung tissues in 3A increase 126% , 69%,36%, and 28% (p

The contents of malondialdehyde(MDA) in the heart,lung and bowel tissues are elevated (P

The effects of oxygen-derived free radicals on the lipid peroxidation injury of the hepatic subcellular organelles were observed in rats after they were inflicted with endotoxic or hemorrhagic shock.It was found that in the early stage of shock,the content of malondialdehyde(MDA)of hepatic mitochondria and lysosome suspension were significantly increased(P

It was found that after 2 dosages of Pseudomonas aeruginosa suspension(7?109/kg and 2?1010/kg)were infused to rabbits respectively to induce septicemia,the elevation of the tumor necrosis factor(TNF)level in the circulating blood was higher in 2?1010/kg group than in 7?109/kg group.The dynamic changes of circulation TNF in P.aeruginosa septicemia were different from those in endotoxin shock.There was a continous increase of plasma glucagon,Iactic acid and ?-G(P

After intravenous infusion of recombinant human tumor necrosis factor(TNF)in the dosage of 60?g/kg or 130?g/kg to rabbits,it was found that their plasma malondialde-hyde(MDA)was significantly increased(P

The effects of endlbtoxin on lipid peroxidation injury of rat hepa-tocytes and the protective effects of exogenous superoxide dismutase (SOD) were studied in vitro.It was found that after the isolated rat hepatocytes and endotoxin (500 ng/ml) were incubated together for 6 hours,there was an obvious increase of malondialdlehyde (MDA) level and lactic dehydrogenase (LDH) and SOD activities,and the increase of LDH and SOD activities occurred earlier than that of MDA level.Polymorphonuclear neutrophils (PMN) after activated by endotoxin could destroy the hepatocytes and increase MDA level and LDH and SOD activities which could partially be inhibited with the pretreatment of exogenous SOD.These findings suggest that endotoxin can intensify the injurious effects of PMN on hepatocytes and it can also directly induce lipid peroxdatipn injury on hepatocyes but the mechanism was not clear.

Changes of hepatic mitochondrial respiratory function and the protective effects of several free radical scavenging enzymes or drugs on the mitochondrial functions were observed after superior mesenteric artery occlusion shock was inflicted to the rat.It was found that there was an obvious decrease of respiratory control rate (RCR) in the 1st hour after the occlusion was released and a further decrease in the 2nd hour,and P/O value decreased significantly at the same time,which indicates that mitochondrial dysfunction does occur.in the shock due to superior mesenteric artery occlusion.However,RCR ard P/O value did rot significantly decrease in the 1 st hour after occlusion releasing in the treated groups with allopurinol (ALLO),a combination of catalase (CAT) and superoxide dismutase (SOD),a combination of SOD and ALLO,and a combination of SOD,ALLO,and CAT as compared with those of the control,and were markedly higher as compared with those of the experimental group.In the 2nd hour after occlusion releasing,RCR of the ALLO treated group was significantly lower than that of the control and remained significantly higher than that of the experimental group.The average survival time of the animals was much longer in the treated groups than in the experimental group.Our findings demonstrate that free radical scavengers,SOD,CAT,and ALLO especially a combinaton of them can exert protective effects of different degrees on the mitochondrial respiratory function during a shock due to superior mesenteric artery occlusion.

Endothelial cells (EC) from human umbilical vein have been subcultured for 10 passages as identified with optical and electron microscopy.The modulation effects of recombinant human tumor necrosis factor (rHTNF and TNF) on the 1st to 3rd generation of ECs in their synthesizing tissue factor were studied.It was found that when rHTNF and ECs were cocultured to the 4th hour,the activity of the tissue factor synthesized by ECs was significantly elevated (P

This paper is to report the determination of the skeletal muscle cellular membrane potential (SMCMP) in vivo with the technique of "Semi-floating"glass microelectrode. The changes of SMCMP and the distribution of extra- and intracellular electrolytes and water in different phases of irreversible hemo-rrhagic shock in rabbits were studied systematically.It was found that SMCMP decreased significantly and immediately as soon as hypotension occurred, which means that the functions of the cellular membrane begin to deteriorate in the early stage of shock; SMCMP continued to decrease when the shock was progressing, which reflects that besides the failure of the cellular membrane function, there is also the degradation of the functions of the cell proper; the leakage of the intracellular potassium across the cellular membrane and the retention of the intracellular sodium and water occurred three hours after the onset of the shock, which indicates that the inability of cells to regulate their volume.

Objective To understand the prevalence,treatment and influence factors of hypertension in maintenance hemodialysis (MHD) patients in Anhui Province.Methods A total of 2724 adult patients on MHD from January 1st 2014 to March 31st 2014 in 26 hospitals of southern,northern and central Anhui Province were investigated.Their demographic characteristics,primary disease,complications,medications,dialysis and laboratory examination were explored.The prevalence treatment rate and control rate of hypertension were analyzed.Associated factors for controlling hypertension [systolic blood pressure (SBP) ＜ 140 mmHg and diastolic blood pressure (DBP) ＜ 90 mmHg] were assessed by logistic regression analysis.Results (1) The prevalence of hypertension in the hemodialysis patients was 87.0％.Their treatment rate and control rate were 93.2％ and 23.9％ respectively.The average of SBP was (145.90±21.18) mmHg,and the DBP on average was (83.60± 12.21) mmHg.The most commonly used anti-hypertensive drug is calcium channel blocker (88.2％).Over one third (45.7％) of patients were treated with two kinds of anti-hypertensive drug,26.2％ with 1 kind,21.7％ with 3 kinds,and 6.4％ with 4 kinds or more.(2) Compared with non-hypertension patients,patients with hypertension have older age,higher body mass index (BMI),phosphorus,SBP and DBP,as well as lower hemoglobin and Kt/V (all P ＜ 0.05).(3) The multivariate logistic regression analysis showed that Ca ＞ 2.50 mmol/L (OR=2.084,95％CI 1.008-4.307,P=0.047) positively correlated with controlling hypertension,while smoke (OR=0.594,95％CI 0.356-0.911,P=0.046) and BMI 18.5 ～ 23.9 kg/m2 (OR=0.516,95％CI 0.293-0.907,P=0.022) negatively correlated with it.Conclusions High prevalence yet low control rate of hypertension in MHD patients in Anhui Province were observed.Hypocalcemia may be a protective factor for hypertension control,while smoke and BMI may be risk factors for it.