Biliary atresia is a disease characterized by progressive inflammation and fibrosis of intrahepatic and extrahepatic bile ducts, which is an important cause of persistent jaundice in infants, Without timely treatment, it can develop rapidly into cirrhosis, and the child will die of liver failure. Therefore, the early diagnosis of biliary atresia is particularly important. The diagnosis of biliary atresia mainly depends on intraoperative cholangiography and liver biopsy, but they have the disadvantages including invasion, complex operation and many complications, which is not conducive to the early diagnosis of biliary atresia. In comparison, some non-invasive examination methods such as laboratory examination and imaging examination have obvious advantages. In this paper, the value of various diagnostic methods of biliary atresia is analyzed, which provides new ideas for its early diagnosis.

Objective To observe the effect of combined administration of Jiang Zhi Tong Luo Soft Capsule(JTSC)and rosu-vastatin on type 2 diabetic with hyperlipemia and its possible mechanisms.Methods Eighty cases of Type 2 diabetic patients with hyperlipemia were divided into combine treatment group (40 cases)and control group (40 cases ).On the basis of routine treat-ment,patients in the combine treatment group were treated by oral 10 mg rosuvastatin (once a day)and oral 100 mg JTSC (three times a day ).Patients in control group were treated by oral 10 mg rosuvastatin (once a day and continuously for 12 weeks).We de-tected the liver function,renal functions,creatine kinase,TC,TG,LDL,HDL and hs-CRP were tested before and after treatments. Results After 12 weeks treatment,the combine treatment group was much better than the control group in reducing the level of TG,TC,LDL,hs-CRP and increasing HDL-C(P <0.05 ).Conclusion Rosuvastatin can effectively treat Type 2 diabetic patients with hyperlipemia.Combined administration of JTSC and rosuvastatin shows better effect than rosuvastatin used alone on the treat-ment of Type 2 diabetic patients with hyperlipemia.

Objective To investigate the effect of miR-21 on the secretion of IFN-γ and IL-17 from peripheral blood mononuclear cells (PBMC) in type 1 diabetic patients (T1D). Methods The expression levels of miR-21 in PBMC from 60 T1D patients and controls by quantitative real-time quantitative polymerase chain reaction (qRT-PCR) were detected and the secretions of IFN-γ and IL-17 from T1D PBMC after transfection with miR-21 were detected by enzyme linked immunosorbent assay. Results The expression of miR-21 in PBMC from T1D patients was significantly lower than the control (P<0.01). The levels of IFN-γand IL-17 from T1D PBMC after transfection with miR-21 were remarkably downregulated compared to the control (P < 0.05 or P < 0.01). Conclusion Compared with that at the healthy control group, the expression of miR-21 in PBMC from T1D patients is decreased. MiR-21 could suppress the secretions of IFN-γand IL-17 from PBMC in T1D patients.

Objective To investigate the time-course of mRNA and protein expression of inducible nitric oxide synthase (iNOS) in pulmonary artery and thoracic aorta and the difference in mRNA and protein expressions of iNOS between pulmonary artery and thoracic aorta induced by endotoxemia in rats. Methods Forty-eight Wistar rats of both sexes weighing 350-450g were randomly divided into 2 groups: control group in which animals received normal saline intraperitoneally (ip) and were killed 3h later by dislocation of cervical vertebrae (n=12); test groups in which animals were injected intraperitoneally lipopolysaccharide (LP) 4mg-kg-1 in 2ml of normal saline and were killed after 3h ( n = 12), 8h ( n = 12) , and 48h( n = 12) . Chest was opened immediately and pulmonary artery (PA) and descending thoracic aorta ( TA) were removed. mRNA and protein expression of iNOS in PA and TA were measured by RT-PCR and Western blot.Results The mRNA and protein expressions of iNOS in PA peaked at 8h and that in TA at 3h after ip LPS injection. The levels of mRNA and protein expressions of iNOS in both PA and TA returned to control level at 48h after ip LPS injection. The levels of mRNA and protein expressions of iNOS in PA at 3h and 8h after ip LPS injection were significantly higher than those in TA.Conclusion The mRNA and protein expressions of iNOS in PA and TA after LPS injection are time-dependent. The mRNA and protein expressions of iNOS in TA after LPS injection are earlier than those in PA but the level of mRNA and protein expressions of iNOS in PA after LPS injection is higher than that in TA.

Objective To review the research advances in molecular biology of vascular restenosis.Methods The literatures about molecular biology of vascular restenosis were reviewed.Results Current transgenic ways had some advantages and disadvantages. Gene therapy with HSV tk, Rb,p21,p27,p53,c myc, c myb, vascular endothelial growth factor,bFGF,platelet derived growth facfor,nuclear factor ?B and so on inhibited vascular restenosis.Conclusion A better transgenic system and gene combination therapy will be effective to treat vascular restenosis.

Objective:To estimate the effect of HSV1-TK/GCV suicide gene therapy on human salivary gland epidermoid carcinoma cell line MEC-1. Methods: Expression vector G 1NAtK containing HSV-TK cDNA was transfected into MEC-1 cells.After transfection, the cells were selected by G418 for two weeks. The integrated gene and mRNA were detected with PCR and RT-PCR. The cytotoxicity and bystander effect were estimated by MTT and typan blue exclusion assay. The morphological changes after GCV treatment were observed with HE and 33258 stain and in situ cell apoptosis detection kit. Results: The 404 bp DNA fragment was amplified through PCR and RT-PCR in the transfected cells respectively. TK positive clone was named MEC-1/TK. The sensitivity of MEC-1/TK to GCV was 1 000 times more than that of parent MEC-1 cells.More than 90% of MEC-1 cells were killed by 10 ?g/ml of GCV when only 10% of MEC-/TK cells were present. The morphological changes included shrinking,detaching and floating of the cells. Some of the cells showed nucleus condensation and breakage of nucleus. A lot of cells showed nucleus positive in in situ apoptosis detection. Conclusion: HSV1-TK/GCV can confer MEC-1/TK cell killing efficiently. MEC-1/TK also has strong bystander effects. HSV1-TK/GCV system confers its effect, in part, by inducing apoptosis in TK positive cells.

Objective:To explore the mechanism of islet cell apoptosis caused by glucose at different concentrations.Methods:The islet cells of SD adult rats were prepared by collagenase digestion,purified by Fcoll400 and cultured in RPMI1640.Different concentrations of glucose or mannitol were added into the monolayer of islet cells.The medium insulin concentration was measured by RIA.The apoptosis rate was measured by the flow cytometry and the bax and bcl-2 expressions were detected by immunoblot.Results:①With glucose concentration raised from 11.1 mmol/L to 22.2 mmol/L,the insulin concentration began to raise and reached the peak,then it began to decline.②With glucose at 11.1 mmol/L and 22.2 mmol/L,the apoptosis rate was the highest,while at 5.5 mmol/L,there was no significant difference compared with the controls. For the groups of mannitol-infused,there was no differences of bax or bcl-2 expressions and no difference of the apoptosis rate comparing with the controls.③With glucose infused,the bcl-2 expressions in 11.1 mmol/L and 22.2 mmol/L groups were negative,bax expression was positive;the bax and bcl-2 expressions were negative in the group of 33.3 mmol/L;the bax and bcl-2 expressions in the 5.5 mmol/L group showed no difference compared with the controls.Conclusion:Higher levels of glucose at 11.1mmol/L and 22.2 mmol/L lead to the release of more insulin and the raise of apoptosis rate.But with glucose concentration at 33.3 mmol/L,the release of insulin decreased and the apoptosis rate declined.Hyperosmolality could not cause the apoptosis of the islet cell.

OBJECTIVE To investigate the distribution of clinical bacterial isolates and the change in antibiotic resistance spectrum in our hospital from 2005 to 2007.METHODS Data of bacterial susceptibility testing of clinical isolates from the Second Affiliated Hospital in of University of South China from 2005 to 2007 were collected and analyzed by software WHONET25.Results were assessed according to the National Committee for Clinical Laboratory Standards(NCCLS) of America issued in 2005.RESULTS The amount of Gram-negative bacteria decreased and of Gram-positive bacteria increased during this period.The proportion of coagulase negative Staphylococcus(CNS) had been increasing and reached 21.7% in 2007.The proportions of Staphylococcus aureus decreased from 17.6% in 2005 to 13.0% in 2007.Escherichia were the top two bacteria in 2007.The drug resistance rate of staphylococci against penicillin and erythromycin was more than 92.2% and 52.2%,respectively.The oxacillin resistance rate of CNS was 74.5%,significantly higher than that of S.aureus(16.5%).Drug resistance rate of Enterococcus to vancomycin was 1.1%.Gram-negative bacteria were found resistant to meropenem and imipenem.The resistance rate to ampicillin of Klebsiella and Escherichia was very high.CONCLUSIONS The variation of drug resistance and distribution of clinical bacterial isolates in our hospital are related to the improper use of antibiotics.It is very important to select antibiotics correctly according to the results of antibiotics susceptibility tests.

ObjectiveTo determine optimal positive end expiratory pressure (PEEP) in mechanical ventilation in children with severe asthmatoid disease based on the quasistatic pressure-volume (P-V) curve.MethodsA serf-control study was done on 23 children with severe asthmatoid disease in the pediatric intensive care unit( PICU ).Quasistatic lung P-V curve of these patients was analyzed and the lower inflection point (LIP) from P-V curve was determined.Three different PEEP (0 cm H2O,LIP,LIP+2 cm H2O,1 mm H2O =0.098 kPa) were given to the patients.The effects of PEEP at different levels on gas exchange,hemodynamic and airway pressure were observed.ResultsThe quasistatic LIP were (2.70 ±2.00)cm H2O.When PEEP was increased to the level of LIP + 2 cm H2O,PaO2 / FiO2 and lung compliance improved significantly (P ＜ 0.01 ) and dynamic lung compliance was the highest,peak inspiratory pressure was (22.30 ± 3.00) cm H2O and mean airway pressure was( 14.11 ± 1.01 ) cm H2O,without obvious adverse effects on mean arterial blood pressure and heart rate.There was no difference in PaCO2,when compared PEEP =0 cmH2O to PEEP =LIP + 2 cmH2O.ConclusionThe application of PEEP is safe.LIP + 2 cm H2O from quasistatic P-V curve could be set as the optimal PEEP under which mechanical ventilation has the best efficacy and do not aggravate CO2 retention and abnormality of hemodynamics in children with severe asthmatoid disease.

Objective To study the influence of probucol on the diabetic mellitus patients with non-proliferative diabetic retinopathy (NPDR) about blood lipids,serum level of the total antioxidant capacity (TAOC),malondialdehyde (MDA),superoxide dismutase (SOD) and macular edema,so as to provide clinical basis for the prevention and treatment of early DR by probucol.Methods 66 type 2 diabetes patients with 127 NPDR eyes were included.Patients were random divided into control and treatment groups,the control group was treated by intensive therapy of blood glucose and blood pressure control,and the treatment group was treated with the intensive therapy and probucol 0.375g,2 times a day for 12 months.Before and after treatment,the blood lipids,oxidative stress indicators and fundus fluorescein angiography in both groups had been determined.Results 62 cases with 120 eyes were enrolled in this study.Probucol obviously decreased levels of total cholesterol (TC) [ (3.6 ± 0.58) mmol/L VS (4.71 ± 0.61)mmol/L,t = 7.65,P ＜ 0.01 ],triglyceride (TG) [ (1.07 ± 0.35) mmol/L VS (1.23 ± 0.43) mmol/L,t = 2.02,P ＜ 0.05 ],low density lipoprotein cholesterol (LDLC) [ (2.0 ± 0.47) mmol/L VS (2.55 ±0.56)mmol/L,t =4.18,P ＜0.01] and MDA[ (10.35 ±2.97)nmol/L VS (14.83 ±2.75)nmol/L,t =6.18,P ＜0.01] in plasma of the patients.Levels of TAOC [(19.25±4.11)u/ml VS (16.63 ±3.27)u/ml,t =3.57,P ＜0.01 ]and SOD[ (94.52 ± 10.28)u/ml VS (75.37 ± 9.87) u/ml,t =8.62,P ＜0.01]were significantly improved in the probucol group,and the macular edema was significantly reduced in patients of the probucol group(x2 =4.219,P ＜0.05).Conclusion Probucol regulated serum lipids,and it had the apparent action of antioxidant,and it decreased the incidence of macular edema.Probucol had a therapeutic effect in patients with NDPR.