1.Screening for Y chromosome azoospermia factor region microdeletions using modified multiplex PCR
Junjie YE ; Hui YAN ; Zongfang LI ; Hai GUO ; Yaoli WANG ; Jiangchuan LI ; Shuhua ZHAO
Chinese Journal of Laboratory Medicine 2010;33(2):111-114
Objective To establish an universal primer-multiplex PCR system for diagnosis of Y chromosome AZF region microdeletions in 262 patients with non-obstructive azoospermic and severe oligozoospermic male infertility. Methods In each panel of multiplex PCR, YUP and YCP containing a fragment of non-human DNA sequence at their 5' ends were designed. The universal primers and chimiric primers were employed for the amplification at the same multiplex PCR system to screen for the Y chromosome AZF region ( a, b and c) microdeletions in 262 non-obstructive azoospermic and severe oligozoospermic male infertility patients. Results Thirty-three out of 262 patients (12. 60% ) were detected with Y chromosome AZF microdeletions. Among them, 27 cases were AZF c microdeletions and 6 ones were AZF b + c microdeletions. These results were in agreement with the results from EMQN method. There was no false-positivity. The gel electrophoresis for detection of multiple STS from both methods showed that the sY84,sY86, sY127, sY134, sY254, sY255, SRY bands were homogeneous and clear with similar brightness. Conclusion The modified multiplex PCR is suitable for screening of Y chromosome AZF microdeletions in non-obstructive azoospermic and severe digozoospermic male infertility patients.
2.The common problems and solution of graduate education in cilinical medicine professional degree
Jiangchuan DONG ; Dilong CHEN ; Qing LI ; Yiting CHEN ; Jing WU ; Yahbin HOU
Chinese Journal of Medical Education Research 2011;10(9):1087-1089
With the size of graduate education continues to expand,deepening the reform of graduate education to improve the quality of graduate education has become the core of graduate education.This paper introduces the common problems and solution of graduate education in cilinical medicine professional degree.
3.Study of macrophage uptake drug and oxygen loaded lipid microbubbles in vitro
Li LIU ; Shufang CHANG ; Jiangchuan SUN ; Mingyue YIN ; Shenyin ZHU ; Yi ZHU ; Zhigang WANG
Chinese Journal of Ultrasonography 2014;23(5):434-437
Objective To investigate the feasibility and parameters for macrophage uptake oxygen and paclitaxel loaded lipid microbubbles (OPLMBs) in vitro.Methods OPLMBs were synthesized by mechanical vibration method and incubated with macrophages in different conditions in vitro.The ratio of macrophages uptake OPLMBs was observed by bright field microscope.Results The ratio of macrophages uptake OPLMBs was closely related to the size,concentration as well as the incubation time of OPLMBs with the macrophage.The ratio of macrophages uptake OPLMBs had a reverse relationship with the size of microbubbles.The highest rate of macrophages uptake OPLMBs was (18.9 ± 0.69)% when the ratio of macrophages to microbubbles was 1 to 10,the time for macrophages co-incubation with microbubbles was 30 min,which was the suitable condition for macrophages uptake OPLMBs.Conclusions OPLMBs can be phagocytized successfully by macrophages in vitro.The size,concentration as well as the incubation time of OPLMBs with the macrophages are important parameters contributed to the efficiency of macrophage phagocytosis.
4.Study on blood lipoprotein(a) in patients with essential hypertension and coronary heart disease
Jing ZHANG ; Luosha ZHAO ; Min GAO ; Fan YANG ; Jiangchuan DU ; Yang LI
Clinical Medicine of China 2011;27(5):471-474
Objective To study the changes of lipoprotein(a)[LP(a)] in patients with essential hypertension(EH) and coronary heart disease(CHD).Methods One hundred and fifty-nine EH older patients were recruited in the study.Eighty-six elderly patients were diagnosed as EH combined with CHD,and 73 patients were diagnosed as simple EH.All patients were tested for the fasting blood glucose(FBG),creatinine(Cr),total cholesterol(TC),triglyceride(TG),HDL-C,LDL-C,LP(a),and the body mass index(BMI) was calculated.Results Plasma Lp(a) increased(0.48±0.18)mmol/L in the EH combined with CHD patients,which were significantly higher than the increasing of(0.34±0.12) mmol/L in the simple EH patients(t=-11.367,P<0.05).The level of plasma Lp(a) increased with the severity of the stenosis of the coronary artery(Lp(a):(0.37±0.14) mmol/L in single arterial branch stenoses,(0.46±0.15)mmol/L in double arterial branch stenoses,(0.66±0.12)mmol/L in triangle arterial branch stenoses,F=31.842,P=0.012).Conclusion The Lp(a) concentration in patients with EH are correlated with the occurrence and severity of coronary heart disease.As a risk of coronary heart disease,Lp(a) can predict the severity of coronary artery stenosis.
5.Low-intensity ultrasound promotes uterine involution following cesarean section.
Dan WANG ; Yi ZHANG ; Li JING ; Wei HE ; Xiaoxu TANG ; Jiangchuan SUN ; Liaoqiong FANG ; Zhibiao WANG ; Hongbo QI
Journal of Southern Medical University 2013;33(2):276-278
OBJECTIVETo observe the clinical effect of low-intensity ultrasound in promoting uterine involution following cesarean section.
METHODSA total of 122 women undergoing cesarean section were randomly selected and divided into low-intensity ultrasound treatment group (67 cases) and control group (55 cases). The women in the treatment group received daily low-intensity ultrasound treatment for 30 min 24 h after the delivery for 3 consecutive days, and the control group had no particular treatments. The descent of the uterine fundus and cessation of lochia was observed in the two groups.
RESULTSThe treatment group showed obviously greater descent of the uterine fundus with a higher rate of cessation of vaginal bleeding than the control group at 30 days postpartum. The ratio of incomplete uterine involution was significantly lower in the treatment group than in the control group.
CONCLUSIONLow-intensity ultrasound is effective in promoting uterine contraction and uterine involution following cesarean section.
Adult ; Cesarean Section ; rehabilitation ; Female ; Humans ; Postpartum Period ; Pregnancy ; Ultrasonic Therapy ; methods ; Ultrasonography ; Uterus ; diagnostic imaging ; Young Adult
6.Talking about the Key Points and Notices of Drug Management in Clinical Trials
Jiangchuan XIE ; Wei GUO ; Linli XIE ; Minghong LI ; Xinmei PAN ; Liya CAO ; Yongchuan CHEN
China Pharmacy 2019;30(21):2894-2898
OBJECTIVE: To provide references for improving the standard operating procedures of drug management in clinical trials and drug management in clinical trials. METHODS: According to Good Clinical Practice (GCP), Data On-site Verification Points of Drugs Clinical Trial, Qualification Examination Rules of Drug Clinical Trial Institution, based on the quality control project carried out in our hospital since July 2016, the matters needing attention in the non-standard operation and key process of drug management in clinical trial were summarized, and the improvement measures were discussed. RESULTS & CONCLUSIONS: Non-standard drug management is a high-incidence link of non-standard operation in the trial process. Among them, the acceptance, distribution and use of drugs are the three links with the highest incidence of non-standard operation of drug management in the trial process. Therefore, when formulating the relevant management system, each institution should pay attention to it according to its own situation; such as, when accepting drugs in clinical trials, attention should be paid to checking the intact degree of drug packaging; drugs transported in cold chain should also be checked for temperature records and rejected in case of over-temperature; the copies of the waybill should be kept in file with the original to avoid fading of the thermosensitive paper; whether the relevant characteristics of the control drugs and placebos meet the requirements. Institutions can standardize the key links of drug management in the trial process, the time of project establishment, project start-up, quality control and supervision, formulate and constantly improve the relevant drug management system and standard operating procedures (SOP). For example, when starting a project, attention should be paid to the participation of drug administrators in the training and signature of start-up meeting, whether the design of the form is complete, standardized and operable. It is necessary for clinical trial institutions to pay attention to the standardization and precision of drug management and the key links in clinical trials.