Objective To investigate the physicochemical properties,structural characters and antitumor activity of a polysaccharide PTPS-1 from Paecilomyces tenuipes.Methods PTPS-1 was obtained by DEAE-cellulose and Sephadex G-100 chromatography column.The methods of HPLC,GC,IR,and NMR were used to investigate the molecular weight,monosaccharide composition,and structural characters of PTPS-1.The CCK-8 kit was used to determine the proliferation of PTPS-1 on murine splenocyte and the supernatant of the murine macrophage with PTPS-1-stimulated on Sp2/0 cells.Results PTPS-1,whose molecular weight was 1.84?104 and purity was 98.977%,contained mannose(Man) and galactogen(Gal) as monosaccharide constitute in molar ratio of 1.3∶1.It consisted of a backbone of ?-(1→6),?-(1→2),and ?-(1→3) linkage,whose branches were made by Gal-(1→3) linkage.PTPS-1 significantly stimulated the murine splenocyte proliferation and the supernatant of macrophage inhibited the growth of Sp2/0 cells in vitro.Conclusion PTPS-1 is a polysaccharide from P.tenuipes with immunomodulatory and antitumor activity.

Objective To investigate the nutritional status of vitamin D and its relationship with age, sex and season in children aged 0-6 in Yiling District of Yichang, and to provide a scientific basis for children to appropriately supplement vitamin D. Methods A total of 11 656 children aged 0 to 6 who had undergone physical examinations in Yiling Maternal and Child Health Hospital from January 2017 to July 2018 were tested for peripheral blood 25-hydroxyvitamin D levels by fluorescence immunochromatography. Results The level of 25-vitamin D in 11,656 children tested in Yiling area was (29.35 ± 7.59) ng / mL, of which 566 (4.86%) were in vitamin D deficiency, 6,579 (56.44%) were in vitamin D insufficiency, and 4,511 were in vitamin D sufficiency (38.70%). The levels of vitamin D in peripheral blood of infants under 1 year old were significantly higher than that of infants and preschool children (P<0.05). The levels of 25-hydroxyvitamin D in peripheral blood of children aged 3-6 years were significantly lower than that of other age groups (P<0.01). The levels of 25-hydroxyvitamin D in children's peripheral blood were highest in spring and lowest in winter. There was no significant difference in peripheral blood levels of 25-hydroxyvitamin D between two genders (t=0.841, P>0.05). The levels of 25-hydroxyvitamin D in peripheral blood aged 0-3 years had no significant difference between urban and rural areas in Yiling area (P>0.05). Conclusion The levels of 25-hydroxyvitamin D in children aged 0-6 years are mostly inadequate. Vitamin D intake should be increased, especially in preschool children. Outdoor activities in winter are necessary in this area.