Objective:To study the environment suitability of multi-parameter patient monitor. Methods: Choosing six different types of multi-parameter patient monitor from different manufactures, we tested six main parameters of the multi-parameter patient monitor when they had gone through the environmental tests, and the test limits come from field environment. Results:Each of the samples was not up to the standards. Conclusion:At present a clear most of the multi-parameter patient monitor in the market are designed and produced for using indoors, which are not suitable for using in field environment. We need to enhance the protection of the multi-parameter patient monitor against field environment, and improve the environment suitability.

With the enforcement of YY 0505-2012, the testing items and evaluation points of radio frequency ablation catheter in electromagnetic compatibility field should be studied and discussed. Based on the requirements of relevant standards, this paper discusses on the testing items that should be applied and the evaluation points that should be focused on by analyzing the intended use and the structure of radio frequency ablation catheter, when it intends to apply registration individually with the basic knowledge of electromagnetic compatibility field.
Catheter Ablation ; instrumentation ; Electromagnetic Fields ; Electromagnetic Phenomena ; Evaluation Studies as Topic

AIM: Granulocyte colony-stimulating factor and stem cell factor are the important cell factor in the hematopoiesis. According to changes in the cell information conduction and obstruction of the expression of the stem cell adhesion molecule, they can increase the quantities of stem cells, restrain the apoptosis and alter the function of bone marrow mesenchymal stem cells. The experiment was to discover how to isolate and culture the granulocyte colony-stimulating factor and stem cell factor mobilized peripheral blood derived mesenchymal stem cells, and to explore the induction to the cartilage cells in the monolayer culture. METHODS: Experiments were performed at the Hebei Provincical Key Laboratory, Research Center, Fourth Hospital of Hebei Medical University from January 2005 to March 2006. ①The 4-6 weeks male BALB/c mice weighting 15-20 g were provided by Animal Experimental Center of Hebei Medical University. The lab procedure met the animal ethical standard. ②The density gradient centrifugation combined with the adherence method were adopted to isolate the mesenchymal stem cells from peripheral blood of BALB/C mice after granulocyte colony-stimulating factor and stem cell factor mobilization. Detections of colony forming unit-forming ability were preformed on grow well cells. Mesenchymal stem cells were identified by flow cytometry and immunohistochemical method. The 3rd passage mesenchymal stem cells were induced to differentiate into chondrocytes in high glucose DMEM medium containing transforming growth factor beta 1. The expression of type Ⅱ collagen was detected by immunohistochemical method. RESULTS: ①The peripheral blood derived mesenchymal stem cells possessed the similar phenotypes with bone marrow derived mesenchymal stem cells and could differentiate into chondrocytes. Flow cytometry showed that CD29 and CD44 were positive, while CD34 and CD45 of them were negative. ②The passage 3 mesenchymal stem cells did not express type Ⅱ collagen and laminin, expressed vimentin in immunohistochemical method. The differentiated mesenchymal stem cells expressed type Ⅱcollagen ③The passage 1,3,5 mesenchymal stem cells showed different ratios of colony forming unit. Pairwise comparison showed that with the passage increasing, the ratio of colony forming unit degraded gradually (21.32%,16.13%,9.63%,P

Mitochondria not only acts a key role in en ergy generating process within the cell, but also is intimately associated with some important even ts in cells, such as apoptosis, generation of reactive oxygen species, lipid met abolism etc. For its special structure and function, mitochondria, as the pharma cological target for many agents, interacts with drugs extensively. Learning the interaction between mitochondria and drugs will greatly contribute to the under standing of the mechanism of the drugs, the design of new agents and the avoidan ce of the drug toxicity and side effect. This review is to present the related p roperties of mitochondria and its interactions with classical mitochondrial-tar geted drugs.

With the aggravation of aging of the population,the incidence of Alzheimer's disease (AD)has been steadily increasing,mild cognitive impairment (MCI)is considered as a cognitive state between aging and dementia.However,the diagnosis of AD and MCI is difficult and mainly depends on the clinical symptoms and the corresponding assessment scales,lacking of the application of biological markers.In this paper,we presented a general review of the biological markers related to AD and MCI,so as to provide reference for their clinical use.

Hepatocellular carcinoma (HCC)is one of the most fatal malignant tumors worldwide.As an important part of cutting-edge re-search fields,proteomics has been widely used in the studies of related diseases and has currently become a crucial experimental approach to research on HCC.Significantly expressed proteins can be identified as potential biomarkers for early diagnosis and targets for therapeutic drugs for HCC.Moreover,they can be used for prediction of the recurrence and prognosis of HCC,as well as for investigation of pathogene-sis of the disease.The proteomic results from worldwide clinical studies of HCC are summarized,and it is suggested that the clinical applica-tion of results of basic research on HCC proteomics will bring great benefit to the diagnosis and treatment of HCC.

As a novel research tool,metabolomics technology can reveal the differences in metabolic profiles during the development and progression of hepatocellular carcinoma (HCC),so it has been widely applied for research on HCC biomarkers.The significance of metabo-lomics in the diagnosis of HCC is briefly described,and the metabolomics research aiming at the discovery of HCC biomarkers,including an-imal experiments and clinical studies of metabolites in the serum,urine,and liver tissue,is reviewed.It is pointed out that analyzing and monitoring metabolites in the development and progression of HCC is of great significance for individualized treatment.

Objective To investigate the effects of Donglian Capsule(the combination of Ophiopogonis Tuber Polysaccharide with Rhizoma Coptidis Alkaloids, OPRA for short, Component of Chinese medicine)on early insulin resistance in experimental diabetic rats. Methods The animal model of insulin resistance in 2 type diabetic was established by injecting low dose of STZ in 60 high fat-fed SD rats. After one week, the successful models were randomly divided into 4 groups: untreated, high and low OPRA-treated, mefformin-treated group, Meanwhile, a normal control group was designed. After four weeks, the differences of FBG, PG, INS and C-P were compared among the groups. Results The OPRA can significantly reduced the level of serum TC and FBG, increased the C-E ]Lightened the IRI(P<0.01). Conclusion The OPRA can reduce the level of serum TG and FBG, and decrease insulin resistance to protect the β-cell function.

HM(hibernating myocardium)is an adaptive phenome-non of myocardium against sustained ischemia,which maintains its tissue vitality through balancing energy supply and demand.It widely exists in patients suffering from coronary heart disease. HMhas its own metabolic pattern,instead of regular FAO(fatty acid β-oxidation)-based metabolism,glycolysis became main pro-cedure.Reduction of FAO,TCA (tricarboxylic cacidcycle),ETC (electron transport chain)enzyme has been observed,ROS(reac-tive oxygen species)and UCP2(uncoupling protein 2)have been up-regulated.UCP2,which promotes proton leak across innermembrane of mitochondrial and leads to ATP reduction,has e-merged as an important regulator of the energy production.It is regulated by up-stream proteins such as AMPK,PPARs,PGC-1α,and other factors like FFA(free fat acid),ROS and purine nucleotide.HM has potential function of ischemic myocardium, which can improve cardiac function through reasonable interven-tion.Modulation of UCP2 can optimize energy production,and is essential to HM metabolism.

AIM:To study the reversal effect of a cyclosporin D analogue PSC833 on multidrug resistance of doxorubicin-resistant human myelogenous leukemia ( K562/DOX) cells.METHODS: The reversal effects of PSC833 on resistance to doxorubicin ( DOX)/vincristine ( VCR) in K562/DOX cells were observed by MTT assay.The cell cycle analysis was performed by flow cytometry.Annexin V/PI staining was used to identify PSC833-induced apoptosis in K562/DOX cells.These cells underwent incubation with DCFH-DA, JC-1 and Fluo-3/AM followed by flow cytometry for the measurement of reactive oxygen species ( ROS) , mitochondrial membrane potential (ΔΨm ) , and intracellular calcium, re-spectively.The protein levels of cytochrome C (Cyt C), Bcl-2, Bax, and cleaved caspase-3 were detected by Western blotting.RESULTS:The DOX/VCR-induced cytotoxicity was significantly potentiated by PSC833.PSC833 arrested the cells in G2/M phase and increased the apoptosis induced by DOX in K562/DOX cells.During the apoptosis, the level of ROS and intracellular calcium increased, while the level ofΔΨm decreased.Furthermore, the release of Cyt C, activation of caspase-3, up-regulation of Bax and down-regulation of Bcl-2 were observed in K562/DOX cells treated with PSC833 and DOX.CONCLUSION: The reversal effect of PSC833 on multidrug resistance in K562/DOX cells is associated with the induction of apoptosis through a mitochondria-dependent pathway.