OBJECTIVE:To improve the quality standard for Jingwu oral liquid.METHODS:The qualitation of Radix Polygoni Multiflori preparata and solomonseal rhizome in Jingwu oral liquid were identified by TLC and the assaying of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside in the oral liquid were determined by HPLC.RESULTS:In the TLCs of Radix Polygoni Multiflori preparata and solomonseal rhizome,the color of speckles was the same as that of the control articles in the corresponding places.The linear range of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside was 0.0 562?g～0.6 744?g(r=0.9 996);The average recovery was 100.1%(RSD=1.89%).CONCLUSION:The method is simple,fast,and reproducible and of high recovery rate,which can be used as quality control for Jingwu oral liquid.

Forecasting ; Quantum Theory ; Toxicology ; trends

Objective To explore the correlation of serum ferritin (SF),fibrinogen(FIB) and D-dimer levels in patients with small vessel disease(SVD).Methods 359 patients with SVD were selected,including 186 patients with lacunar infarction ( LI) and 173 patients with leukoaraiosis ( LA) .176 healthy controls were enrolled in this study as control group.The SF,FIB and D-dimer concentrations were measured by radioimmunoassay method and Clauss method respectively .Results The serum levels of SF , FIB and D-dimer in SVD patients were significantly higher than those in the controls [(171.82 ±156.28)μg/L vs (100.37 ±70.11)μg/L,(3.27 ±0.93) g/L vs (2.97 ± 0.88)g/L,(1.10 ±1.06)mg/L vs (0.83 ±0.55)mg/L](all P<0.05).Compared with LI subgroup,the levels of SF,FIB and D-dimer were higher in LA subgroup [(185.22 ±108.55)μg/L vs (159.36 ±89.69)μg/L,(3.32 ± 0.86)g/L vs (3.23 ±0.99)g/L,(1.29 ±1.17)mg/L vs (1.03 ±0.80)mg/L],but the differences were not signifi-cant(all P<0.05).Multivariate regression analysis revealed that by comparison to the reference quartile ,the odds ra-tio (95%CI) for SVD patients in the higher quartile (SF>151.16μg/L,D-Dimer>1.02 mg/L) were 3.444(95%CI=2.097-5.158,P<0.001) and 1.767(95%CI=1.114-2.803,P=0.016),respectively after multivariable ad-justment.Conclusion The increased levels of SF ,FIB and D-dimer are closely related with SVD .FIB and D-dimer might come to be attractive approaches to prevent the progress of SVD .

Circulating tumor markers have been paid more attention in the application of the treatment for breast cancer, the level of which has extended from protein to gene, including traditional tumor markers, HER-2 extracellular domain, circulating tumor cells, circulating tumor DNA (ctDNA), circulating RNA (ctRNA) and so on. As “liquid detection”, the detection of circulating tumor markers with real-time dynamic, easy operation, good reproducibility and other advantages are widely used in aiding early diagnosis, determining prognosis, prospectively predicting response or resistance to speciifc therapies, surveillance after primary surgery, and monitoring therapy in patients with advanced disease, The further study of circulating tumor markers may contribute to patient’s individual treatment.

A high performance liquid chromatographic method with hypersil C_(18) column, 0.020 mol/L potassium dihydrogen phosphate solution(which contains 0.25% triethylamine, pH 5.1)-methanol(20∶ 80, V/V) as mobile phase and fluorescence detection with 280 nm as excitation wavelength and 320 nm as emission wavelength has been developed for the determination of terbutaline sulfate and propranolol hydrochloride in Erythrocyte suspensions. Erythrocyte surface receptor oscillating reacted 1 h with terbutaline in 37 ℃ water bath. Free terbutaline and propranolol were separated from the membrane receptor-binding complex through 1500 r/min centrifugal in 5 min. The linear range was 0.010-3.000 mg/L in Alsever's solutions and the correlation coefficients were 0.9999 and 0.9998. The relative standard deviations(RSDs) of terbutaline and propranolol were 0.8% and 1.2%, respectively. The limits of detection(LODs) of both were 1.00 and 3.00 mg/L. This simple, safety and sensitive method was suitable for the determination of terbutaline and propranolol in erythrocyte suspensions and for the study of receptor-ligand binding.

Sulfotransferase (SULT) enzymes play important roles in the biosynthesis of estrogen.These enzymes catalyze the sulfation of estrogens to form biologically inactive molecules. Several studies have shown that Arg213His polymorphism in SULT1A1 gene may be a risk factor for breast cancer. In addition, a low mRNA expression of SULT has been significantly associated with reduced recurrence and improved overall survival in patients with estrogen receptor (ER)-positive tumors. Furthermore, SULT may be a crucial factor in the response to tamoxifen treatment. As an important part of estrogen synthetic pathways, SULTs are closely re lated to breast cancer.

Objective To investigate good nutritional measurements of CKD patients.In order to diagnose malnutrition early in CKD patients.Methods Clinical data of 125 CKD patients have been collected.The glomerular filtration rate(GFR)was estimated from the modification of diet in renal disease(MDRD).According to the results of GFR,all CKD patients were divided into five groups.The levels of biochemical markers were assayed.At the same time,bioelectrical impedance analysis(BIA)were measured.We analyzed the relationship between nutritional parameters and GFR.Results According to SGA,malnutrition rate in fifth CKD stage is 29%.Impedance of leg is significantly correlated to GFR in 1kHz,50kHz and 500kHz,impedance of truck is also significantly correlated to GFR in 50kHz and 500kHz.The values of impedance reduced with the decreasing of GFR.Conclusion SGA and BIA all can be used to evaluate nutritional status of CKD patients.BIA is a more sensitive method to detect early malnutrition and may be useful in monitoring nutrition changes in CKD patients.

Objective To observe the clinical effect of card other tromethamine for postpartum hemorrhage. Methods 80 cases with postpartum hemorrhage were randomly divided into the observation group and control group, 40 cases in each group.The control group was given oxytocin treatment.While the observation group was given oxyto-cin and card other tromethamine treatment.The clinical curative effect,bleeding time after treatment,postpartum 2h haemorrhage amount and adverse reaction,etc.were observed and compared.Results The total effective rate of the observation group was 95.0%,which was significantly higher than 75.0% of the control group,the difference was sta-tistically significant(χ2 =15.68,P <0.05).The haemorrhage amount of postpartum 2h of the observation group was (273.7 ±10.5)mL,which was significantly lower than (547.1 ±16.4)mL of the control group,the difference was statistically significant(t =88.79,P <0.05).The bleeding time of the observation group was (14.8 ±3.4)min,which was significantly shorter than (34.9 ±8.4)min of the control group,the difference was statistically significant (t =14.02,P <0.05).The incidence rate of adverse reactions of the observation group was 7.5%,that of the control group was 5.0%,there was no statistically significant difference between the two groups(χ2 =0.53,P >0.05 ). Conclusion Card other tromethamine in the treatment of postpartum hemorrhage has good clinical curative effect and high safety.

Objective:To explore the application value of continuous positive airway pressure ( CPAP) in the treatment of obstruc-tive sleep apnea hypopnea syndrome (OSAHS ) complicated with coronary heart disease (CHD).Methods:Totally 50 patients with OSAHS complicated with CHD from June 2012 to June 2014 in our hospital were randomly divided into the control group and CPAP group with 25 ones in each .The patients in the control group were treated with the conventional drug therapy for coronary heart disease , while those in CPAP group were treated with CPAP additionally .After 6-month treatment, PSG index, clinical symptoms, arrhythmia and ischemia electrocardiogram changes of the patients in the two groups were compared .Results:After the 6-month treatment , AHI and oxygen desaturation index in CPAP group were significantly decreased when compared with those before the treatment , and the min-imum value of SaO2 and the average value of SaO 2 were higher than those before the treatment (P<0.01), and the above indices were better than those in the control group (P<0.01).The incidence rate of chest pain and chest tightness , arrhythmia, times of myocardi-al ischemia in a day and time of myocardial ischemia in a day were decreased after the treatment (P<0.01), and the decrease in CPAP group was more notable than that in the control group (P<0.01).Conclusion: CPAP combined with the conventional drug therapy for coronary heart disease can improve the oxygen concentration and reduce the incidence of arrhythmia and myocardial ischemia in the patients with OSAHS with CHD , which can be widely used in clinics .

Objective To predict the potential targets of apigenin by virtual screening .Methods The targets preliminarily forecast by PharmMapper ,were validated by associating data mining and Autodock Vina in PyRx 0 .8 .Subsequently ,receptor‐ligand interactions were analyzed by Discovery Studio 3 .5 .Results The virtual screening by PharmMapper indicated that apigenin coupled well with the disease‐related targets including insulin receptor ,estradiol 17‐beta‐dehydrogenase 1 ,and cathepsin K .According to the data mining ,insulin receptor was found in related experimental researches ,while the other two had few reports previously .And then ,the interactions between apigenin and the target proteins were analyzed by Autodock Vina and Discovery Studio Visualizer 3 .5 ,involving hydrogen bonds ,electrostatic forces ,van der Waals forces etc .Conclusion The most potential targets of apigenin were insulin receptor ,while 17‐beta‐dehydrogenase 1 and cathepsin K were also possible .