OBJECTIVE:To improve the quality standard for Jingwu oral liquid.METHODS:The qualitation of Radix Polygoni Multiflori preparata and solomonseal rhizome in Jingwu oral liquid were identified by TLC and the assaying of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside in the oral liquid were determined by HPLC.RESULTS:In the TLCs of Radix Polygoni Multiflori preparata and solomonseal rhizome,the color of speckles was the same as that of the control articles in the corresponding places.The linear range of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside was 0.0 562?g～0.6 744?g(r=0.9 996);The average recovery was 100.1%(RSD=1.89%).CONCLUSION:The method is simple,fast,and reproducible and of high recovery rate,which can be used as quality control for Jingwu oral liquid.

Objective To evaluate the clinical effectiveness of laparoscopic cholecystectomy ( LC) combined with endoscopic sphincter-otomy ( EST) for treatment of gallstones with common bile duct stones. Methods Clinical data of 652 patients who were treated by LCs and ESTs were retrospectively analyzed. Results ESTs were successfully accomplished in 634 patients (97. 2%), and LCs were successfully ac-complished in 621 among the 634 patients (97. 9%) after ESTs. The mean length of hospitalization after LCs was 4 days (2~15 days). In all 652 patients, there were 233 cases of acute pancreatitis, including 171 cases before ESTs and 62 cases (13. 1%) after ESTs, and they were all cured after ESTs. In 621 patients after LCs, bile leakages occurred in 8 patients (1. 2%) and they were cured with intraoperative drainages. Local fluid accumulations occurred in 120 patients (19. 0%), and 118 cases of them healed without management, and the other 2 cases were cured with percutaneous tube-placement drainages guided by ultrasonography because of abscesses. Conclusion It is safe and effective to treat gallstones with common bile duct stones by LC combined with EST because of its high success rate, short hospital stay and mild complications.

Objective To investigate the effect of acceptance and implementation therapy on anxiety and depression in cancer patients'spouse. Methods The spouses of 50 hospitalized patients from January to June 2014 were assigned into the control group and the spouses of another 50 cases of hospitalized patients from July to December 2014 into the observation group . All the patients received routine care methods. The control group received general health education and the spouses in the observation group were managed with acceptance and implementation therapy, totally for 3 times. After the intervention, the self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used to evaluate the levels of anxiety and depressions in both groups. Results Before intervention, there were no differences in SAS and SDS scores between the groups (P>0.05). After intervention, the SAS scores in the observation group and the control group scores were (48.36 ± 6.28) and (55.11 ± 6.74), respectively, with statistically significant difference (t=7.530, P<0.05). The SDS scores of the observation group and control group were (50.03 ± 4.12) and (58.50 ± 4.63) respectively, with statistically significant differences (t=6.672, P<0.05). The SAS and SDS score of observation group were lower than those before intervention (t = 7.283,7.524, all P < 0.05). Conclusion The acceptance and implementation therapy can effectively alleviate the anxiety and depression in the spouses of those patients with cancer.

Objective To predict the potential targets of apigenin by virtual screening .Methods The targets preliminarily forecast by PharmMapper ,were validated by associating data mining and Autodock Vina in PyRx 0 .8 .Subsequently ,receptor‐ligand interactions were analyzed by Discovery Studio 3 .5 .Results The virtual screening by PharmMapper indicated that apigenin coupled well with the disease‐related targets including insulin receptor ,estradiol 17‐beta‐dehydrogenase 1 ,and cathepsin K .According to the data mining ,insulin receptor was found in related experimental researches ,while the other two had few reports previously .And then ,the interactions between apigenin and the target proteins were analyzed by Autodock Vina and Discovery Studio Visualizer 3 .5 ,involving hydrogen bonds ,electrostatic forces ,van der Waals forces etc .Conclusion The most potential targets of apigenin were insulin receptor ,while 17‐beta‐dehydrogenase 1 and cathepsin K were also possible .

Objective:To explore the application value of continuous positive airway pressure ( CPAP) in the treatment of obstruc-tive sleep apnea hypopnea syndrome (OSAHS ) complicated with coronary heart disease (CHD).Methods:Totally 50 patients with OSAHS complicated with CHD from June 2012 to June 2014 in our hospital were randomly divided into the control group and CPAP group with 25 ones in each .The patients in the control group were treated with the conventional drug therapy for coronary heart disease , while those in CPAP group were treated with CPAP additionally .After 6-month treatment, PSG index, clinical symptoms, arrhythmia and ischemia electrocardiogram changes of the patients in the two groups were compared .Results:After the 6-month treatment , AHI and oxygen desaturation index in CPAP group were significantly decreased when compared with those before the treatment , and the min-imum value of SaO2 and the average value of SaO 2 were higher than those before the treatment (P<0.01), and the above indices were better than those in the control group (P<0.01).The incidence rate of chest pain and chest tightness , arrhythmia, times of myocardi-al ischemia in a day and time of myocardial ischemia in a day were decreased after the treatment (P<0.01), and the decrease in CPAP group was more notable than that in the control group (P<0.01).Conclusion: CPAP combined with the conventional drug therapy for coronary heart disease can improve the oxygen concentration and reduce the incidence of arrhythmia and myocardial ischemia in the patients with OSAHS with CHD , which can be widely used in clinics .

Melatonin has a significant inhibitory effect on various cancers,especially on breast cancer.In estrogen receptor (ER)-positive human breast cancer,anti-cancer effects ofmelatonin on breast cancer cells and transplanted tumors mainly achieve by suppressing ER mRNA expression and ER transcriptional activity via the MT1 receptor.In addition,melatonin regulates the transactivation of other members of the nuclear receptor super-family,estrogen metabolizing enzymes,and the expression of related genes.Furthermore,melatonin also suppresses tumor aerobic metabolism,critical cell-signaling pathways relevant to cell proliferation,survival,metastasis,and drug resistance.Melatonin demonstrates both cytostatic and cytotoxic activity in breast cancer cells that appears to be cell-type specific.Studies on animal and human models indicate that disruption of the circadian nocturnal melatonin signal promotes the growth,metabolism,and signaling of human breast cancer,resulting in invalid hormone therapy and chemotherapeutic resistance in breast tumors.

Objective To analyze the composing principles of the prescriptions for Lijie disease by using The Prescription of Traditional Chinese Medicine Dictionary. Methods The prescriptions for Lijie disease in the book of The Prescription of Traditional Chinese Medicine Dictionary were collected. Database was established by using TCM Inheritance Support System (V1.1). Medicine using frequency, composing principles, and composition of new medicine were analyzed through association rules and entropy clustering method. Results Based on the analysis on 187 cases of prescriptions, 42 Chinese herbal medicines were with the frequency of 42 times and Angelicae Sinensis Radix was the core herbal medicine. 69 core combinations with three to four herbal medicines and 6 new prescriptions were obtained through evolution. Conclusion According to the analysis of composing principles and clustering new prescriptions of Lijie disease, the treatment for Lijie disease is toning liver and spleen, and nourishing liver and kidney.

Objective To explore the correlation of serum ferritin (SF),fibrinogen(FIB) and D-dimer levels in patients with small vessel disease(SVD).Methods 359 patients with SVD were selected,including 186 patients with lacunar infarction ( LI) and 173 patients with leukoaraiosis ( LA) .176 healthy controls were enrolled in this study as control group.The SF,FIB and D-dimer concentrations were measured by radioimmunoassay method and Clauss method respectively .Results The serum levels of SF , FIB and D-dimer in SVD patients were significantly higher than those in the controls [(171.82 ±156.28)μg/L vs (100.37 ±70.11)μg/L,(3.27 ±0.93) g/L vs (2.97 ± 0.88)g/L,(1.10 ±1.06)mg/L vs (0.83 ±0.55)mg/L](all P<0.05).Compared with LI subgroup,the levels of SF,FIB and D-dimer were higher in LA subgroup [(185.22 ±108.55)μg/L vs (159.36 ±89.69)μg/L,(3.32 ± 0.86)g/L vs (3.23 ±0.99)g/L,(1.29 ±1.17)mg/L vs (1.03 ±0.80)mg/L],but the differences were not signifi-cant(all P<0.05).Multivariate regression analysis revealed that by comparison to the reference quartile ,the odds ra-tio (95%CI) for SVD patients in the higher quartile (SF>151.16μg/L,D-Dimer>1.02 mg/L) were 3.444(95%CI=2.097-5.158,P<0.001) and 1.767(95%CI=1.114-2.803,P=0.016),respectively after multivariable ad-justment.Conclusion The increased levels of SF ,FIB and D-dimer are closely related with SVD .FIB and D-dimer might come to be attractive approaches to prevent the progress of SVD .

Neoadjuvant systemic therapy for breast cancer patients means systemic treatments before operations.Combination chemotherapy,endocrine therapy and targeted therapy before operation can degrade the tumor staging and increase the breast-conserving rates.Neoadjuvant chemotherapy has been accepted as one of the standard therapies for patients with locally advanced breast cancers.In the neoadjuvant setting,the studies about the use of aromatase inhibitors and trastuzumab in selected patients have also made great progresses.

Circulating tumor markers have been paid more attention in the application of the treatment for breast cancer, the level of which has extended from protein to gene, including traditional tumor markers, HER-2 extracellular domain, circulating tumor cells, circulating tumor DNA (ctDNA), circulating RNA (ctRNA) and so on. As “liquid detection”, the detection of circulating tumor markers with real-time dynamic, easy operation, good reproducibility and other advantages are widely used in aiding early diagnosis, determining prognosis, prospectively predicting response or resistance to speciifc therapies, surveillance after primary surgery, and monitoring therapy in patients with advanced disease, The further study of circulating tumor markers may contribute to patient’s individual treatment.