Objective To study the changes of ICAM-1 expression in transplanted lung at the early stage after rat pulmonary transplantation.Methods The expression level of ICAM-1 protein and its mRNA in rat transplanted lung after 4 h of ventilation and reperfussion were detected by using immunohisto chemical method(SABC method)and semiquantitative reverse transcriptase polymerase reaction method in the experimental group(n=6).When donor lungs had been flushed with low potassium solution(LPDS) and preserved in 4.C LPDS for 12 h,left orthotopic pulmonary transplantation was performed.In control group (n=6), left pulmonary artery,pulmonary vein and bronchi were fully freed to be naked.Immunohistochemical test results were recorded in negative(-),suspicion(±),faint positive(+),positive(++)and intensive positive(+++).PCR products were separated by agrose gels and the density of the bands were determined by density scanning.Results The stained color of alveolar epithelial cells and pulmonary vascular endothdial cells in the transplanted lung was significantly more intensive in experimental group than in control group(P＜0.01) and the relative density values(ICAM-1/βactin)were also significandy higher in experimental group(0.837±0.044) than in control group (0.442±0.037),P＜0.01. Conclusions ICAM-1 expression in the transplanted lung was up-regulated in the early stage after pulmonary transplantation,which was related with the enhancement of ICAM-1 mRNA.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

Objective To explore the efficacy of ligustrazine combined with chemotherapy in patients with relapsed or refractory NHL and the relationships with P-gp expression. Methods 60 Patients were randomized into reversal agent group (ligustrazine + chemotherapy) and control group (chemotherapy) and the outcome was analysed. P- gp expression was analysed by flow cytometry. Subgroup analysis was conducted a ccording to P-gp expression. Results 56 patients could be evaluated. Progression -free survival (PFS) showed no statistical diference in two groups ( P = 0.065 1 ) ; reversal agent group had a higher overall response rate (ORR) than control group(P=0. 048).Subgroup analysis showed 41 patients had P-gp( +). The complete remission rate and complete remission uncertain rate of patients with P-gp( + ) in reversal agent group and control group were 33. 33% and 8. 70% , respectively, reversal agent group had a significant advantage ( P = 0. 048). Patients with P-gp( + ) in reversal agent group had a higher ORR than control group (61. 11% vs 26. 09% ,P =0.024). Kaplan-Meier survival curve and Log-rank test showed patients with P-gp( + ) in reversal agent group had longer PFS than control group (P =0.046 4). Blood pressure in a small number of patients received ligustrazine therapy decreased,and mitigated after reducing the dose. Conclusion Ligustrazine combined with chemotherapy can elevate response rate,prolong PFS with manageable toxicity and correlate to P-gp expression in relapsed or refractory NHL

Epidemiologic evidence suggests that diabetes mellitus appears to be negatively associated with abdominal aortic aneurysm formation. Diabetes mellitus may affect the physiological characteristics of the aorta and reduce the prevalence, incidence and expansion rate of abdominal aortic aneurysm. But the impact of diabetes mellitus on the rupture, operative complication and clinical outcome of abdominal aortic aneurysm is unclear. Randomized controlled trials are needed before conclusions can be drawn on the link between development and expansion of abdominal aortic aneurysm and medicines for diabetes. This review aims to analyze updated evidence of the association between diabetes mellitus and abdominal aortic aneurysm.

Objective To investigate the value of procalcitonin in guiding the antibiotic use in acute exacerbation of chronic ob-structive pulmonary disease(AECOPD) .Methods A total of 184 patients with AECOPD in our hospital from March 2014 to Sep-tember 2015 were selected and divide into two groups by the random number table method .Ninety-two cases in the control group received the antibiotic therapy according to clinical experience .Ninety-two cases in the observation group was implemented the anti-biotic therapy according to serum procalcitonin level .Results Compared with before treatment ,the procalcitonin level after treat-ment in the observation group was decreased significantly (P<0 .05) .The procalcitonin level after treatment ,occurrence rate of a-cute exacerbation within six months and re-hospitalization rate within six months in the observation group were lower than those in the control group(P<0 .05) .The antibiotic use time ,antibiotic expenses ,hospitalization time and hospitalization cost in the obser-vation group were less than those in the control group(P<0 .05) .The total effective rate and hospitalization mortality rate had no statistical difference between the two groups (P>0 .05) .Conclusion Serum procalcitonin is significantly increased in the patients with AECOPD .Procalcitonin can be used for the optimized use of antibiotics .

This study reviews the researches on self-handicapping in about more than 20 years. It summarizes the practical researches of self-handicapping from 7 aspects: (1)manifestations and types of self-handicapping behaviors;(2)evaluative conditions that typically elicit self-handicapping;(3)motives underlying self-handicapping; (4)factors influencing on self-handicapping; (5)consequences of self-handicapping; (6) measurements of self-handicapping. On this basis, it raises some questions in self-handicappping researches and points out the future direction.

Objective: To explore the mediation of stress between perfectionism and life satisfaction.Methods: 317 college students were surveyed with questionnaires.The data were analyzed by hierarchical regression.Results: ①Regarding general perfectionism,both male and female students' stress fully mediated negative perfectionism and life satisfaction but did not mediate positive perfectionism and life satisfaction;②Regarding domain-specific perfectionism,male students' appearance stress fully mediated related perfectionism and satisfaction,but for female students its mediation was partial.Both male and female students' personality stress fully mediated personality perfectionism and satisfaction.Mediation models involved academic,love,interpersonal relationship perfectionism and their related satisfaction were not found.Conclusion: The mediations of stress between perfectionism and life satisfaction are different in different life domains.

OBJECTIVE: To investigate the tagging condition in medication of special population in package inserts in order to give reference for rational drug use of special population. METHODS: 389 pieces of common Chinese and western medicine package inserts of our inpatient and out-patient dispensaries were randomly collected and analyzed statistically in terms of the content of package inserts for special population, including pregnant and lactant women, children and the aged. RESULTS: A part of medication items of special population in package inserts had deficiency. According to the classification of dosage form, the tagging rate of injection exceeded other dosage forms reached to more than 90%, except of medication items for children. The tagging rate of oral formulation arrived at more than 90% only in terms of contraindications and cautions. According to the classification of manufacturer, the tagging rate of domestic drugs was kept at the low level while that of imported drugs exceeded 90%. CONCLUSIONS: Package inserts need to be improved with the effort of drug supervision management department, drug production department and population of using drugs to guarantee the safety of drug use in special population.

We obtained human IL-2R cDNA 673 bp fragments from plasmid pJSB15 and prepared ?-32 p-IL-2 R cDNA probe. Dot hybridizations of RNA extracted from human lymphocytes were done by using the probe. The results showed that PHA or TPA could induce IL-2 R mRNA, respectively; and the synergistic effects were seen when used them together. IL-2R mRNA could be expressed spontaneously in many kinds of leukemic lymphocytes isolated freshly from PBL of leukemic patients.

Objective To investigate the value of expressed prostatic secretion (EPS) leukocyte count in the diagnosis of chronic prostatitis. Methods EPS was obtained from 75 out patients with chronic prostatitis,pH and WBC per high power microscopic field being studied.Transabdominal prostatic ultrasonography was performed. Results Mean pH of the EPS was 6.9?0.4.pH of the EPS and the frequency of ultrasound abnomalities were significanly correlated to the leukocyte count in EPS.There was no correlation between age of symptom duration and the pH of EPS, frequency of ultrasound abnormalities or white blood cell counts in EPS. Conclusions In chronic prostatitis,the leukocyte count in EPS was positively correlated with the ultrasound abnomalities and the pH elevation of EPS.There was no correlation between age or symptom duration and pH of the EPS,frequency of ultrasound abnormalities or leukocyte count in the EPS.