1.Comparison of Recombinant Human ?-NGF from E.coli and CHO in Biochemical Characters
Jing JIANG ; Gui-Xiang JIANG ;
China Biotechnology 2006;0(02):-
Though prokaryotic cells could hardly express recombinant human beta nerve growth factor (rhNGF-?) with a proper three-dimensional conformation, using of E. coli as a host for industrial production of rhNGF-? is controversial. Recombinant human beta NGF was expressed in E. coli and was refolded in vitro. The isolated products was shown to be consistent with those expressed and secreted by CHO cells in biochemical characters by SDS-PAGE, RP-HPLC, mass spectrometry, N terminal analysis and bioassay determined using DRG and PC12 cells. The products can be acquired with 95% purity, 1.8 ng/U biological activity from both expression system,which remain invariable biological activity when lyophilized in excipient and store at 37℃?2℃, RH 75%?5% for 3 months. Moreover, the product refolded from inclusion bodies of E. coli shows the predominance in homogeneity and the lower cost.
2.Application of ultrasound enhanced thrombolysis in patients with acute ischemic stroke
International Journal of Cerebrovascular Diseases 2012;(12):943-947
This article introduces the development history of ultrasound-assisted thrombolysis technology and operating points.It rainly focuses on the research status and application prospects of the technology in the treatment of acute cerebral infarction.
3.Advances in Study on Evaluation of Functional Dyspepsia by Dyspepsia Symptom Scale and Quality of Life Questionnaire
Chinese Journal of Gastroenterology 2015;(3):180-183
Functional dyspepsia(FD)is a commonly seen gastrointestinal disease. Dyspepsia symptom scale and quality of life questionnaire are two objective approaches for evaluating the efficacy of intervention measures on FD. This article reviewed the advances in study on evaluation of FD by dyspepsia symptom scale and quality of life questionnaire.
4.Molecular abnormality of cardiocyte mitochondrial membrane in diabetic cardiomyopathy
Journal of Medical Postgraduates 2003;0(09):-
Diabetic cardiomyopathy is one of the main causes of death in diabetic patients.Mitochondrial dysfunction plays a key role in the development of diabetic cardiomyopathy,and mitochondrial function depends on the stabilization of the mitochondrial membrane structure.Studies on the molecular alteration and the underlying mechanism of the cardiocyte mitochondrial membrane of the diabetic heart may give us deeper insights into diabetic cardiomyopathy,and help us make a breakthrough in the therapeutic strategies.
5.Congenital muscular dystrophy type 1A: a report of one case with literature review
Journal of Clinical Pediatrics 2017;35(5):369-371
Objective To investigate the clinical features and genetic tests of a case with congenital muscular dystrophy type 1A (MDC1A). Methods Clinical data of proband were collected, and genetic change were tested using next generation sequencing, and literatures pertinent to the epidemiology, mechanisms, especially genetic testing of lisencephaly were reviewed. Results A 5 year and 2 month old boy present with normal intelligence and delayed motor development, he can be sit alone but not walk at two years old. Physical examination showed normal mental reaction, muscular dystrophy, hypotonia, and joint contracture at early age. From biochemical tests, we found creatine kinase (CK) and CK-MB were increased (491U/L, 41.8U/L). EMG test suggested possible muscle-derived damage. Brain MRI showed white matter abnormality. And a heterozygous mutation (c.2045-2046delAG) inherited from his mother in LAMA2 gene, and another novel heterozygous mutation (del Exon5) inherited from his father were identified by genetic test. Conclusions LAMA2 gene deficiency can lead to MDC1A, and gene testing can help diagnosis.
6.Design and implementation of materials management system for hospital warehouse of combat readiness based on RFID
China Medical Equipment 2017;14(8):108-111
Objective:To develop a materials management information system for military hospital warehouse of combat readiness based on RFID so as to enhance the management of military medical corps for materials of war preparation.Methods:RFID(Radio Frequency Identification) tags were attached on war materials and the information could been read by bar-code reader. SQL Server 2005 was used as the backend database, and VC# was combined with RFID to develop the software system.Results: This research has developed a materials management information system for military hospital warehouse of combat readiness based on RFID and this system could achieve informatization for information management, management of out and in warehouse, emergency plans management and forewarning management. Through manoeuvre, the average time of war materiel out warehouse has saved 12min.Conclusion: The research and development of information management system for materiel information of hospital warehouse for combat readiness has reference value for the application of researching RBID technique in material management.
7.Acute myeloid leukemia.
Chinese Journal of Hematology 2013;34(2):174-174
8.Delayed postoperative infection in deep site of fracture of lumbar vertebrae treated through anterior approach: a case report.
Gang-xiang WANG ; Xiang-jiang ZHU ; Hai-dong ZHOU
China Journal of Orthopaedics and Traumatology 2015;28(1):55-57
Adult
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Humans
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Lumbar Vertebrae
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injuries
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Male
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Spinal Fractures
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surgery
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Surgical Wound Infection
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therapy
10.Experimental Study on Liq Qibei against Herpes Simplex Virus-Ⅱ in Vitro
Hongyi DENG ; Huizhong JIANG ; Xiang YU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(10):-
Objective To explore the effect and mechanism of Liq Qibei against herpes simplex virus-Ⅱ(HSV-Ⅱ) for treatment of cervical erosion.Methods Using micro-inhibition cytopathic and in vitro cell culture technique,the various concentrations of Liq Qibei in inhibiting HSV-Ⅱ proliferation and the direct virucidal effect were studied.The virus titer and virus inhibition index were taken as parameters.Results ① Virus proliferation experiment:Compared with control group,the virus titer of 1.63,0.81,0.41 mg/mL Liq Qibei group had significant difference(P2.② Direct virucidal effect of Liq Qibei:comparing with control group,1.63 mg/mL Liq Qibei in 8 h,24 h and 0.81 mg/mL-24 h groups had marked difference(P0.05).Conclusion Liq Qibei antivirus effect on HSV-Ⅱ.