Current risk classification in childhood B-cell precursor acute lymphocytic leukemia has been used to modulate the intensity of treatment and prognosis assessment.However there are a significant proportion of relapses and deaths occur among childhood with good risk clinical characteristics and/or an excellent early response to therapy.So research about the aberrant expression of gene associated with relapse will improve cure rate.Aberrant expression of CRLF2 gene can be found in childhood B-cell precursor acute lymphocytic leukemia and associated with JAK aberrantion,BCR-ABL1-like signature,IKZF deletion.Over-expression of CRLF2 gene is associated with high risk relapse and poor outcome in childhood B-cell precursor acute lymphocytic leukemia,but its prognostic value is still controversial.This article mainly reviews the progress of CRLF2 gene in childhood B-cell precursor acute lymphocytic leukemia.

EVI1 gene mainly controls embryo development.3q rearrangement,MLL translocations,monosomy 7,which react with other genes,can lead to EVI1 aberrant expresion or fusion gene.Recent studies have shown aberrant expression of EVI1 gene in parts of acute lymphatic leukemia,acute myeloid leukemia and chronic granulocytic Leukemia and correlation with poor prognosis.Leukemogemc mechanism include epigenetic modifications,transcriptional control,regulation of signaling pathways,upregulation of cell adhesion,proliferation,and colony formation and antiapoptosis.Drugs of genetic therapy include epigenetic agents,mTOR inhibitor,human ITGA6/ITGB4 complex antibody and CD52 monoclonal antibody.

Objective To study the effect of alcohol on apoptosis and apoptosis-associated genes-Bcl-2 and Bax of cardiac muscle cell.Methods We established an animal experimental model by giving different concentration of alcohol to the healthy mice.Myocardial apoptosis and the expression of apoptosis-associated gene Bcl-2 and Bax were quantitatively analysed by the nuclear fast red-crystal violet staining and immunohistochemical method.Results Some nuclears of the experimental groups were stained purple and agglutinated.Counting under the high power microscope,it was found that the amount of abnormal nuclears significantly increased in alcohol-treated groups compared with the control group (P

Objective:To study VEGF-C expression in colon cancer and the relationship of VEGF-C expression with clinicopathological features and prognosis.Methods:VEGF-C expression was examined by immnohistochemical staining in 44 cases of primary colon cancers.To analyze the relation of VEGF-C expression with clinicopathological features and prognosis.Results:Of the 44 cases of colon cancers,19 cases of carcinoma (43.2%) were evaluated to be VEGF-C positive expression and 25 cases of carcinoma (56.8%) were determined to be VEGF-C negative expression.VEGF-C expression was associated with tumor differentiation (P=0.003),nodal status (P=0.002) ,Dukes stage (P=0.001).The median survival time of the patients with positive VEGF-C expression was lower than that of patients with negative VEGF-C expression (P=0.0225).Metastatic status,the nodal status and VEGF-C were found to be significant independent prognostic factors (P=0.021,P=0.0004,P=0.0469,respectively).Conclusion:VEGF-C expression is upregulated in colon cancer.VEGF-C expression plays a important role in nodal metastasis.VEGF-C appears to be a new prognostic factor for the prediction of outcome in patients with colon cancer.

Objective To explore the relationship of pregnancy-associated plasma protein-A (PAPPA),osteoprotegerin (OPG) and endometriosis,so as to apply the new theoretical basis for the etiology of endometriosis.Method PAPP-A and OPG in peritoneal fluid were measured in 25 women with endometriosis(study group) and 25 women with hysteromyoma ablation (control group).Results The levels of PAPP-A and OPG were both higher in peritoneal fluid in study group [(72.3±57.4)U/L and (3.28±2.63) μ g/L]than that in control group [(42.3± 19.7) U/L and (1.73 ±0.45) μ g/L] (P＜0.05).Furthermore,significant correlation was found between the stage of endometriosis and the levels of PAPP-A and OPG in study group (P＜0.05).The concentration of PAPP-A in peritoneal fluid were significantly higher in the secretory phase than the proliferative phase of the menstrual cycle in two groups(P＜0.05) ,and OPG in peritoneal fluid without significant difference(P＞ 0.05).Conclusion PAPP-A and OPG maybe play an important role in the development of endometriosis.

Objective: To make an impersonal evaluation on the effect of TCM on main biochemical indexes of non-alcoholic fatty liver disease (NAFLD),including ALT,AST,GGT,TC,TG,and HDL-C.Methods: We searched all the randomized controlled trials (RCTs) of TCM for NAFLD from CBM, CNKI,and VIP (1999-2008).RevMan4.2 was used in the analysis. Results: 11 RCTs,including 1078 patients,met the inclusion criteria.All of the trials were not adequate in methodological quality. None of them was double-blinded trial.The result of analysis showed that compared with basic Western medicine,TCM could increase the content of HDL-C,reduce the content of ALT,AST,GGT,TC and TG.Conclusion: The present meta-analysis suggested that TCM was beneficial over Western medicine for NAFLD which indexes including ALT,AST,GGT,TC,TG,and HDL-C.But we can not make a final conclusion due to low quality of RCT.

Objectives To study the effects of anesthesia, head-up position and CO 2 pneumoperitoneum on cardiac autonomic nerve evaluated by heart rate variability (HRV) during laparoscopic cholecystectomy. Methods 20 patients undergoing elective laparoscopic cholecystectomy were anesthetized with balanced anesthesia. Pneumoperitoneum was introduced by inflation of CO 2. Intra-abdominal pressure was maintained at (11～13) mmHg. HRV was measured in supine and head-up tilt (15?～20?) positions while the patients were awake, in the stable state of anesthesia and CO 2 pneumoperitoneum at 10 minute and 20 minute respectively. Power spectral analysis of HRV was conducted on 256-beat R-R interval segments of interpolated heart rate by using the fast Fourier transformation. Total spectral power (TP), low frequency power (LF) and high frequency power (HF) were calculated by integrating the power spectra between (0～0 5) Hz, (0 03～0 15)Hz and ( 0 15～0 35)Hz. Results The change of positions did not significantly influence HRV. Decreases in LF, HF and TP were noted during the maintenance of anesthesia. The power of LF, the normalized unit of LF and LF/HF ratio increased during inflation period. LF/HF increased from 2 15?1 16 to 4 61?2 04 ( q =4 491, P

OBJECTIVE To investigate the risk factors of nosocomial infection in elderly lung cancer inpatients, to determine reasonable protective and therapeutic measures. METHODS Sixty-eight nosocomial infection cases and 215 non-nosocomial infection cases in elderly lung cancer inpatients were analyzed retrospectively. RESULTS The data of the TNM stage (?2=14.883,P=0.001), pathologic type (?2=34.217,P=0.000) and pathogenic site (?2=15.288,P=0.000) showed significant differences. By Logistic regression analysis, ECOG, antimicrobial treatment, hospitalization and hypoproteinemia showed significant differences(P