The systematic teaching does better to help students master and use what they have learned, and it will be good for avoidance of repeated contents and shortening teaching time. It can also help students find more interest in studying neurology.

Gene expressions of interleukin-6 receptor subunit gp130 and gp80 were investigated by competitive RT-PCR in the rat model of osteoporosis. A significant increase in expressions of gp130 and gp80 was found in bone marrow of osteoporotic rat, this change may be a possible mechanism in osteoporosis due to loss of estrogen.

Objective To upgrade ordinary gastrointestinal machine to a digital one which can be connected to PACS.Methods By adding extra camera lens,frame grabber and processing software,ordinary gastrointestinal machine was transformed.Results The updated machine could work in digital way with fully DICOM support.Conclusion By analyzing the X-ray imaging chain,a digitalization method of gastrointestinal machine is presented,which can server as a guideline to digital transformation of non-digital X-ray machines.

Objective:To investigate the effect of catgut implantation at acupoint,Compound Erxian Decoction on levels of serum cytokine in ovariectomized rats.Methods:Sixty 3-month-old female Sprague-drawley rats,weighed 230?20g each,were divided into six groups,ten rats in each group.The first group were sham-operated(Sham-G),while the remaining groups,established by excising both sides ovary,were catgut-acupuncture group(CAG),Chinese herb group(CHG),catgut-acupuncture plus Chinese herb group(CCG),estrogen group(EG)and model group(MG).There wasn’t any therapy in sham-operated group and model group.After 12 weeks corresponding therapy,all groups were sacrificed to procure blood serum for testing the contents of E 2 ,GM-CSF,IGF-1,TNF-?by means of radioimmunoassay and both retral thighbone for testing bone mineral density(BMD) and bone mineral content(BMC)in each specimen.Results:The BMD and BMC of thighbone,E 2 and IGF-1 of serum and in CAG,CHG,CCG and EG was compared with Sham-G,there was no significant difference,but which was higher than MG(P

Objective To explore the effect of δ-opioid receptor (DOR)on ischemic tolerance of rat brain.Methods The focal ischemic tolerance models of Sprague Dawley rats were established using the twice suture method with the middle cerebral artery occlusion (MCAO).A total of 24 male Sprague Dawley rats were randomly divided into sham group,ischemia (MCAO) group,sham +ischemia (sham+ MCAO) group and ischemic preconditioning + ischemia (IP+ MCAO) group (n=6,each).The neurological status was assessed using Zea-Longa neurological deficit scores at 7 days after cerebral infarction.The mRNA expressions of DOR,Bax,Bcl-2 in hippocampus in ischemic rat brain were detected by RT-PCR method.Results The Zea-Longa neurological deficit scores were 0.0±0.0,2.6±0.5,2.8±0.6 and 1.5±0.6 in Sham group,MCAO group,Sham+MCAO group and IP+ MCAO group,respectively at 7 days after cerebral infarction.The scores had significant differences among MCAO group,Sham+ MCAO group and IP+ MCAO group (both P＜0.01),but no difference between MCAO group and Sham+MCAO group(P＞0.05).The mRNA expressions of DOR and Bcl-2 were higher and Bax mRNA expression was lower in IP+MCAO group than in MCAO and Sham+ MCAO groups (all P＜0.01).Conclusions Ischemic preconditioning may increase the mRNA expressions of DOR and Bcl-2,reduce Bax mRNA expression,and improve the neurological status in rats.

BACKGROUND: Light-curing composite resins have been applied in the dental repair due to its beautiful color, excellent physical and chemical properties and easy to operation. However, its insufficient mechanical properties tend to cause composite fractures, resulting in undesired clinical efficacy.OBJECTIVE: To investigate the preparation, properties and biomechanical performances of filler-co-augmented photo-curable resin-based oral materials.METHODS: The nano-silica surface-grafted with poly(methyl methacrylate) (PMMA) was obtained by atom transfer radical polymerization. Co-electrospinning was used to prepare the acrylonitrile/PMMA core-shell nanofibers, and a two-dimensional lactic acid-glycolic acid copolymer nanofiber membrane with a lattice structure was obtained using a copper mesh as a receiving device. The multi-scale and multi-dimensional packing was prepared by sol-precipitation method with silane coupling agent as a raw ethyl ester precursor, and further modified using silane coupling agent. The mechanical properties, volumetric shrinkage, toxicity, and degradation properties of the light-curing resin grafted with SiO2-PMMA were compared with those of the light-curing resin combined with trapezoidal polysiloxane materials grafted with methyl methacrylate. RESULTS AND CONCLUSION: (1) Characterization of the composite resin under scanning electron microscope: the filler SiO2-PMMA core-shell nanofibers dispersed well in the light-curing resin matrix, in the presence of monodisperse phenomenon and less aggregation phenomenon. However, the trapezoidal polysiloxane material in the light-curing resin matrix dispersed unevenly, in the presence of reunion phenomenon. (2) The flexural strength, flexural modulus and fracture work of the light-curing resin graftedwith SiO2-PMMA core-shell nanofibers were significantly higher than those of the trapezoidal polysiloxane-based light-curing resin (P < 0.05). (3) The volume shrinkage of the light-curing resin grafted with SiO2-PMMA core-shell nanofibers was lower than that of the trapezoidal polysiloxane-based light-curing resin (P < 0.05). (4) Compared with the trapezoidal polysiloxane-based light-curing resin, the water absorption and cytotoxicity (absorbance value) of the light-curing resin grafted with SiO2-PMMA core-shell nanofibers were significantly higher than those of the trapezoidal polysiloxane-based light-curing resin (P < 0.05), while the solubility of the light-curing resin grafted with SiO2-PMMA core-shell nanofibers was lower (P < 0.05). It is concluded that the prepared light-curing resin grafted with SiO2-PMMA core-shell nanofibers has excellent properties and biomechanical properties.

Objective To investigate the effect of hypoxia on the e xp ression and function of integrin receptor ? v? 3 of bovine retinal vascular endotheliocytes. Methods Bovine retinal vascular endotheliocy tes in the culture dishes coated by vitronectin was put into the normal and hypo xemic condition, respectively. Enzyme linked immunosorbent assay and cel l-adhesion analysis were used to detect the expression and function of integrin receptor ? v? 3 in bovine retinal vascular endotheliocytes, respectively. Results Under the condition of hypoxia, the expression of ? v? 3 increased gradually, and reached the peak at the 48th hour. The expressio n of ? v? 3 at the 60th and 72nd hour in hypoxia group was higher than that in the normal group. Bovine retinal vascular endotheliocytes absorbed more Vn of extra-cellular matrixes (ECM) after cultured under hypoxemic condition for 24 hours. Conclusion Hypoxia may up-regulate the expression of ? v? 3, which promote the adsorbability of endotheliocytes.

Objective To explore the effects of Zhaoke defibrase and anti-? v? 3 mAb (23C6) on the adhesion and immigration of bovine retinal vascular endothelial cells. Methods The culture dishes coated with vitronectin (Vn) and collagen,assays of adhesion and immigration were performed 60 minutes after different concentration of Zhaoke defibrase and anti-? v? 3 mAb was added to the bovine retinal vascular endothelial cells. The apoptosis of bovine retinal vascular endothelial cells induced by Zhaoke defibrase and anti-? v? 3 mAb was detected by electron microscopy. Results Both Zhaoke defibrase and anti-? v? 3 mAb inhibited the adhesion and immigration of bovine retinal vascular endothelial cells in a dose-dependent manner. The inhibited concentration (IC 50) of Zhaoke defibrase was less than 0.05 ?mol/L, while IC 50 of anti-? v? 3 mAb was more than 2.5 ?mol/L. 81.8% endothelial cells adhering to Vn were inhibited by 0 1 ?mol/L Zhaoke defibrase, while 76.3% by endothelial cells adhering to Vn were inhibited by 10 ?mol/L anti-? v? 3 mAb. Typical apoptosis cells were found in bovine retinal vascular endothelial cells after affected by Zhaoke defibrase and anti-? v? 3 mAb. Conclusion Both Zhaoke defibrase and anti-? v? 3 mAb can significantly inhibit the adhesion and immigration of bovine retinal vascular endothelial cells to extracellular matrix, and the mechanism may lie in inducing the apoptosis of endothelial cells.

0.05).Conclusion The significantly reduced serum IGFBP-3 level is helpful for the diagnosis of HCC,especially in patients without chronic hepatitis and cirrhosis.