OBJECTIVE To investigate the infection status and drug-resistance of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) isolated from female genitourinary tract.METHODS A total of 1890 female genitourinary tract specimens were collected from Jan 2003 to Dec 2006.The results of mycoplasma cultivation and susceptibility test were analyzed retrospectively.RESULTS Among 1890 specimens,there were 756 with positive mycoplasma cultivation and the positive rate was 40.0%.The positive ratio was the highest at the age of 21 to 40.From 12 commonly used antibiotics,the sensitivity to tetracyclines was the highest,and then was to macrolides.Four quinolones all showed the higher resistance,and their sensitivity rates were all below 50%.CONCLUSIONS The drug-resistance of mycoplasma in female genitourinary tract is greatly severe.The clinical doctors should think highly of it.

Objective To explore the clinical effects of sitagliptin alone or its combination with metformin on the patients with type 2 diabetes registered on their first clinic/hospital visits.Methods Eightytwo patients with type 2 diabetes who diagnosed with oral glucose tolerance test (OGTT),insulin test and glucagons stimulating C-peptide test from May 2011 to February 2012 in our hospital were randomly divided into two groups:the patients in control group took sitagliptin alone (control group,n =41) and the patients in experimental group took sitagliptin combined with metformin (experimental group,n =41).Eighty-two patients,after the mission,were given diabetic diet and moderate exercise therapy.Taking sitagliptin 100 mg,1 times/d;combined with metformin groups given sitagliptin 100 mg/d,metformin 250 mg,3 times/d.Six months later,the major indexes comparing between before and after treatment of weight,body Mass Index (BMI),glycosylated hemoglobin(HbA1c),OGTT,fasting plasma glucose (FPG),2 h plasma glucose (2 hPG),fasting insulin (FINS),2 h postprandial insulin (2 hINS),fasting C-peptide and 2 h postprandial C-peptide levels were measured to research the changes during the treatments.Results After treatments,all the indexes were decreased significantly,and the BMI,HbA1c,FPG,2 hPG,FINS,2 hINS,fasting C-peptide and 2 h postprandial C-peptide in the group with sitagliptin united with metformin were significantly lower than the group with sitagliptin((27.4 ± 1.2) kg/m2 vs.(27.9 ±1.5) kg/m2,t =-2.812;(8.1 ±1.2)％ vs.(8.8 ± 1.3)％,t =-2.953;(8.17 ±1.22) mmol/L vs.(9.22 ± 1.28) mmol/L,t =-4.940;(10.16 ± 1.68) mmol/L vs.(11.01 ± 1.62) mmol/L,t =-3.362; (9.22 ± 1.93) mU/L vs.(9.85 ± 1.59) mU/L,t =-2.212;(74.62±13.73) mU/L vs.(83.75 ± 13.12) mU/L,t =-5.235;(1.43 ±0.34) μg/L vs.(1.77 ±0.27)μg/L,t =-3.194; (6.80 ± 1.40) μg/L vs.(7.61 ± 1.64) μg/L,t =-4.480 ; P ＜ 0.05).Conclusion Sitagliptin is useful to treat type Ⅱ diabetes,plays important roles in improving patients' quality of life and insulin resistance,control of blood glucose,reduce the family and the burden of social health,and the effect will be more better when united with metformin.

MicroRNA(miRNA) is involved in virtually all biologic processes, including cellular proliferation, apoptosis, and differentiation. Thus, miRNA deregulation often results in impaired cellular function and disease development, so miRNAs have potential therapeutic relevance. The elucidation of these processes regulated by miRNAs and the identification of novel miRNA targets in the pathogenesis of hypertension is a highly valuable and exciting strategy that may eventually led to the development of novel treatment approaches for hypertension. Several mechanisms have been implicated in the pathogenesis of hypertension: overactivation of therenin-angiotensin-aldosterone system (RAAS), dysfunction of the vascular endothelium, damnification of vascular smooth muscle. To maintain and restore target organ expression of miRNA stable may be a new strategy for treatment of hypertension. The article reviews pathogenesis of miRNA and hypertension, researches of miRNAs as biomarker and therapeutic target, discusses advances in miRNA-based approaches that may be important in treating hypertension.
Animals ; Biomarkers ; metabolism ; Humans ; Hypertension ; drug therapy ; genetics ; metabolism ; MicroRNAs ; genetics ; metabolism ; Molecular Targeted Therapy

Chemotherapy treatment plays an important role in the comprehensive treatment of malignant disease,but the chemotherapy related knowledge and the selection of an appropriate regimen for certain patient are hard to master.The establishment of computer-assisted cancer chemotherapy program and management system with a follow-up database of cancer patients can help the oncologist to master the designing skill of chemotherapy regimen and cancer-related knowledge quickly,improve the teaching qnality and the efficiency of treating malignant diseases.

Objective The purpose of this study was to investigate the effect of different level of sex steroids (?-estradiol and progesterone) on the proliferation of uterus endometrial epithelial cells. Methods Rabbit uterus endometrial epithelial cells were isolated by digestion of trypsin and centrifugation. The specificity and homogeneity of the endometrial epithelial cells cultured in media containing ?-estradiol and progesterone were determined by immunofluorescence staining of cytokeratin monoclonal antibody and the flow cytometric analysis. The different concentration of progesterone (P4) and ?-estradiol (E2) were added in media, the proliferative capability of the endometrial epithelial cells cells in vitro was determined indirectly by MTT assay. Results High purity endometrial epithelial cells (96%) were isolated using centrifugation method. The endometrial epithelial cells were examined by immunofluorescence staining. The endometrial epithelial cells were positive stained by anti-cytokeratin antibody. Immunofluorescence staining demonstrated that cytokeratin was detectable in the cytoplasm of epithelial cells. The cell growth curve showed that the proliferation of endometrial epithelial cells was not significantly changed by the treatment of E2 (100nmol/L) or P4 (100nmol/L) individually, but a combination of 100nmol/L E2 and 10nmol/L P4 could promote its proliferation greatly (P

Objective To study the effects of propofol pretreatment on S100? and neurosecretion enzyme (NSE) of brain tissues with global cerebral ischemia-reperfusion in rat, and to evaluate the effects of propofol in protection of brain. Methods 30 SD male rats were randomly divided into 3 groups: sham operation group (group A, n=10); single cerebral ischemia-reperfusion group (group B, n=10); propofol pretreatment at 2h before ischemia group (group C, n=10), in which propofol (100mg/kg) was given intraperitoneally (ip) before ischemia. 24h after ischemia-reperfusion, the neuroethology scores were recorded and evaluated, and S100? and NSE in rat brain were determined. Results The neuroethology scores of group A were higher than those of group B (P

There are many etiologies, which can produce dysbacteriosis. Among these etiologies which have serious influence and are commone clinically is prolonged, large and abuse of antibiotics.The principles of prevention and cure for dysbacteriosis are presented as follows: A. Reason-able use of antibiotics: (1) Short-term treatment with small dose; (2) Whenver possible, using nar-rowspectrum and different broadsprectram antibiotics ; ( 3 ) Avoiding taking orally; ( 4 ) Selective decontamination. B. Recommending usage of bio-gen and its growth factors.

Objective To evaluate the relationship between the carotid atheroscalerosis and coronary artery disease. Methods High frequency ultrasonography was used to examine the carotid artery in seventy two patients who had undergone coronary artery angiography because of acute myocardiac infarction, angina or chest pain. They were divided a group with normal coronary angiography and another group with coronary artery lesion. The latter group included three subgroups according to the number of coronary artery involved: B1 group, B2 group, and B3 group. Scoring of atherosclerosis of the common carotid artery, internal carotid artery and external carotid artery were made with reference to plaques, the intima media thickness (IMT) and the V max of blood flow of the carotid artery as demonstrated by high frequency ultrasonograply. Results The IMT of the group with coronary lesion was significantly higher than that of the normal group ( P

objective To evaluate the relationship between the pulse pressure and the cardiac geometric structure in patients with essential hypertension. Methods Three hundred and ninety-five patients,age ranging from 20 to 77 years,were enrolled into the study. Their clinical systolic and diastolic blood pressure (SBP/DBP) were all above 140/90mmHg. All the patients either were initially diagnosed as having essential hypertension,or confirmed after taking a the placebo for 2 weeks after stopping anti-hypertensive drugs. The cardiac geometric structure was determined by the echocardiography at the same time when the patients untook the 24 hours ambulatory blood pressure monitoring. Results The clinical pulse pressure was significantly different among the groups as divided by the age ( P

Objective To investigate the effect of potassium iodide on the incidence of sporotrichosis, and provide the theoretical basis for the prevention of sporotrichosis. Methods Experimental mice were divided into 4 groups, group 1～3 were fed with water containing different doses of potassium iodide, and no potassium iodide was given to the control group. After two weeks, the fungi were inoculated intraperitoneally to all the mice. In the third to the eighth weeks, the mice were sacrificed every week in batches. The infection, pathological changes and mycological findings of the mice were observed. Results The incidence rate of experimental sporotrichosis was significantly decreased in the mice (P