We transformed a E.coli—yeast shuttle plasmie containing human interleukin 2 gene into a yeast strain Saccharomyces cerevisiae 8534—8c,then hybredized the transformants with another yeast strain Saccharomyces cerevisiae CSH 83—L to get diploid hy brids The hap Joid transfor-mants and diploid gybreds were compared for their expression level of recombinant interleukin 2.We also studied the gybrids'rIL—2 expression under various conditions and its fermentation.

By means of cDNA amplified fragment length polymorphism(cDNA-AFLP) technique, a fragment P1708 was amplified from Polima cytoplasmic male sterile Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinenesis Makino, syn, B. rapa L. ssp. chinenesis) 'Bpol97-05A'. RT-PCR showed that this fragment was specifically expressed in male sterile material. Sequencing and BLAST search in GenBank database indicated that P1708 had 100% homolog with chloroplast ndhJ-trnF gene region except a 54 bp insertion. Gene specific primer pairs were synthesized according to ndhJ-trnF gene region and two fragments about 1 900 bp were amplified respectively using genomic DNA templates of Polima cabbage and male fertile oilseed rape. The sequencing results showed that the gene region ndhJ-trnF of Polima cabbage contained two 54 bp repeats and some variation sites. The repeat part shared the same sequence as trnF gene except three bases at 5′ ends. For the insertion of 108 bp sequence, a new open reading frame was created.

Objective To observe the effects of continuous blood purification(CBP) combined with early enteral nutrition (EEN) in the treatment of acute severe acute pancreatitis(SAP) complicating hyperlipidemia.Methods Fifty-six cases of acute SAP complicating hyperlipidemia in our hospital from January 2014 to October 2015 were selected as the research subjects and divided into the CBP plus EEN group(combined group) and blood purification(BP) group according to the random digital table method,28 cases in each group.The CBP group was treated with CBP,while on the basis of CBP group the combined group was combined with EEN therapy (within 2 d after admission).The clinical efficacy and the improvement situation of various indexes after treatment were observed in the two groups.Results After treatment,the total effective rate in the combined group was 96.43 ％,which was higher than 75％ in the CBP group,the difference was statistically significant (P＜0.05);the related indicators of TG,UAMY,LPS,SAMY,CRP and LDH after treatment in the two groups were decreased,but which in the combined group were more significant compared with the CBP group(P＜0.05).After treatment,the APACHE-[[score in the combined group was significantly lower than that in the CBP group(P＜0.05),the symptom relief time and hospital stay time in the combined group were shorter than those in the CBP group (P＜0.05).The incidence rate of complications in the combined group was 10.71％,which was significantly lower than 28.57％ in the CBP group,the difference was statistically significant(P＜0.05).Conclusion CBP combined with EEN can significantly improve the prognosis of SAP patients and effectively reduces the complications.

ObjectiveTo validate the judgment criteria of the validity scale for the assessment system on the character and temperament approach of command officers,and to describe the theory of this scale.MethodsUsing the way of imitating and field testing,correction of the criteria was verdicted based on the binary distribution theory.Results ①According the imitating research,about the 22 pairs of couple-items,the correct ratio,which is completely based on guessing,is about 4.9％,not reach 5％.②According the field testing,about the 22 pairs of couple-items,the correct ratio,which is completely based on guessing,is about 7.6％,not reach 8％.ConclusionOn the basis of theory analyzing,imitating research and field testing,not only can find the couple-items scale has many good features,such as more various show,more excellent disguise,but also can affirm the criterion of 19,which is based on the binary distribution theory,is also appropriate.

Objective To explore the correlation between the glucose variability and the death of critically ill patients in neurology department.Methods Clinical characteristics of 231 patients admitted to neurointensive care unit (N-ICU) were analyzed retrospectively.The patients were divided into two groups:survival group(n =190)and death group (n =41).The baseline data,such as gender,age,mechanical ventilation,acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score were recorded.All data of blood glucose measured during hospitalization in N-ICU were collected.Standard deviation (SD),coefficient of variability (CV),maximum successive change and mean amplitude of glycemic excursions (MAGE) were used as glucose variability index.Logistic regression analysis was used to study the association between the glucose variability and the death.Receiver operating characteristic (ROC) curve was adopted to evaluate the application value of each glucose variability index on predicting mortality.Results Glucose variability,APACHE Ⅱ score (deleting the age component) and mechanical ventilation were significantly correlated with death (OR =4.959,1.444,5.472 ; 95％ CI 2.015 ～ 12.202,1.240 ～ 1.681,1.455 ～20.574;P =0.000,0.000,0.012).The predictive value was high and same when using SD and APACHE Ⅱ score (deleting the age component) as predictor (area under the ROC curve =0.910,0.957 ;Z =1.396,P =0.163).The application value of CV,maximum successive change and MAGE was moderate (area under the curve =0.847,0.856,0.872).Conclusion There is significant association between the glucose variability and the death of critically ill patients in neurology department.

Objective To investigate the clinical correlation of intestinal bacterial translocation(BT) and in-testinal barrier of pediatric intestinal surgery,and to observe the relationship between BT and postoperative infection.Methods The plasma D-lactic acid,LPS level of 61 children with intestinal surgery in our hospital were detected before and after surgery.Polymerase chain reaction (PCR) was used to test blood bacterial DNA,in order to judge whether there is BT.Results The PCR results of preoperative children were negative,the PCR positive for 3 cases.According to the PCR results,postoperative systemic inflammatory response syndrome(SIRS) and complications of infection of PCR positive group were obviously higher than those of PCR negative group(P ＜ 0.01).And plasma D-lactic acid,LPS inspection of PCR positive group were obviously higher than PCR negative group(P ＜ 0.01).Conclusion Intestinal barrier damage has close relationship with BT in children after intestinal surgery,SIRS and infection complications also has a direct relationship with BT.

Carbapenem-resistance is an emerging problem in Chinese neonatal intensive care units.Carbapenem-resistant enterobacteriaceae(CRE)can hydrolyze almost all β-lactam antibiotics including carbapenems by producing carbapenemase.There are three groups of carbapenemases, namely Amber A, B and D groups, which have different hydrolytic activities to specific β-lactam antibiotics.Currently, Chinese NICUs have been facing high colonization and infection rates of CRE, with high fatality rate and rapid transmission.The treatment of neonatal CRE infections is extremely difficult.The limited choice of antibiotics, the lack of pharmacokinetic and pharmacodynamic data and the uncertainty of the optimal dose and interval bring great challenges to the effective therapy of neonatal CRE infections.The main antimicrobial agents for CRE in adults and children include carbapenems, ceftazidime/averbactam, fosfomycin, polymyxin, aztreonam, etc., but there are few studies in neonates.Once infants are colonized or infected by CRE, decolonization and treatment are very difficult.Therefore, strict implementation of infection control and neonatal antimicrobial stewardship programs to reduce CRE production, transmission and infection, are the most important measures to cope with the prevalence of CRE.

OBJECTIVE: To review the outcomes of experiments about vascular endothelial growth factor(VEGF) and the effect of it on bone formation, and to make sure whether VEGF can promote bone formation or not.DATA SOURCES: Electronic data searches were performed to obtain data from the databases of http://www.ncbi. nlm. nih. gov/pubmed and http://www. zglckf.com. DATA SELECZION: Nearly 30 articles about VEGF and the role of VEGF affecting bone formation were selected, regardless of the randomized design,blind method involved in or not.DATA EXTRACTION: These researches proved the structures, biologic properties and expression of VEGF and vascular endothelial growth factor receptor(VEGFR), and showed that VEGF plays an important role in endochondral ossification by promoting angiogenesis, bone turnover and preventing apoptosis of chondrocyte. In intramembranous ossification, absence of cartilage, osteoblasts are likely producing, and responding to, VEGF. VEGF also acts to recruit and activate osteoclasts as well as stimulate osteoblast chemotaxis, differentiation and matrix mineralization. It is debatable whether local application of exogenous VEGF can promote bone repair or not. However, in certain situation such as ischemia, the increase of VEGF locally may promote bone repair.DATA SYNTHESIS: Compared with the control group, VEGF can promote osteogenesis, bone formation and remodeling through the effect of VEGF on endochondral ossification and intramembranous ossification.CONCLUSION: One possible advantage of local VEGF therapy may be its ability to couple angiogenesis with bone formation and remodeling. If we can ultimately apply VEGF to treat osteonecrosis, bone defect and nonunion, we will find a new therapy for these diseases.

BACKGROUND: Icari n as one of the main components of Epimedium has an inhibitory effect on osteoclasts.. OBJECTIVE: To further investigate the influence of icariin on the root absorption of the maxil ary first molar at mesial part during orthodontic treatment in rats. METHODS: Orthodontic root resorption models were established in the left maxil a of rats. Local injection of 200 mg/kg icari n (icari n group) or normal saline (positive control group) was administrated into the left first molar buccal periosteum. The right maxil a of rats served as negative control group that was treated with neither appliance nor drug injection. The mesial distance between bilateral first molars and the contralateral maxil ary incisor was measured before and after the appliance was placed. Mesial surface of the mesial root of bilateral maxil ary first molars was observed using scanning electron microscope. RESULTS AND CONCLUSION: Mesial movement of the maxil ary molars in the icari n group was significantly less than that in the positive control group (P < 0.05). Under the scanning electron microscope, smal absorption lacunae were scattered in the icari n group, while the positive control group showed a large amount of absorption lacunae and they were interconnected into a sheet, showing a stark contrast with the smooth root surface of the negative control group. It is indicated that icari n can inhibit root resorption caused by orthodontic treatment, while reducing the amount of mesial movement of the molar under corrective force.

Cardiac Catheterization ; standards ; Heart Defects, Congenital ; therapy ; Humans