Helicobacter pylori infection is a common gastrointestinal infection,which is a major cause of chronic gastritis,peptic ulcer,stomach cancer and gastric mucosa-associated lymphoid tissue lymphoma and other diseases.In recent years,many studies show that Helicobacter pylori infection is closely related with many diseases,which may cause the retardation of children development and affect blood lipids and glucose metabolism.Helicobacter pylori infection may also lead to chronic inflammatory process,results in a large number of cytokines and play an important role in artery atherosclerosis.This paper reviews on the correlation between Helicobacter pylori infection and metabolic diseases and its mechanism as well.

ObjectiveTo investigate the pain relieving degree and the comprehensive efficacy of pricking-cupping bloodletting plus microwave therapy in treating intractable tennis elbow.MethodSeventy-two patients meeting the inclusion criteria were randomly allocated, using a random number table, to a treatment group (pricking-cupping bloodletting plus microwave therapy,n=36) and a control group (electroacupuncture plus microwave therapy,n=36). The pain relieving degree and the overall therapeutic effect were assessed using the Visual Analogue Scale (VAS) and theCriteria of Syndrome Diagnosis and Therapeutic Effect in Traditional Chinese Medicinein the patients after treatment.ResultThe pain relieving degree and the overall therapeutic effect were more remarkable in the treatment group than in the control group (P<0.05).ConclusionPricking-cupping bloodletting plus microwave therapy can effectively heighten the pain relieving degree and the overall therapeutic effect in treating patients with intractable tennis elbow.

Objective To develop secondary nursing discipline system based on the situation of China and the international trends of nursing education,which would be applied as the theoretical guideline for the construction of nursing system in China.Methods An indicator system of entry criteria of secondary nursing discipline was formed by examining the current situation of nursing knowledge body and organizational system in domestic and foreign countries through bibliometric and content analysis,were further supported in the panel meeting.Then the secondary nursing discipline system were constructed according to the entry criteria.Results Knowledge innovation,knowledge dissemination,personnel training and social needs of health were found to be the first indicators of the entry criteria,including 14 secondary indicators,10 secondary nursing disciplines were proposed.Conclusions The secondary nursing discipline system is in line with current situation of nursing at home and abroad,and would be gradually refined with the de velopment of nursing discipline.

AIM:To explore the changes and the mechanism of heart functions in the rats with spleen-qi defi-ciency syndrome.METHODS:The rats were randomly divided into blank control group and spleen-qi deficiency model group.The changes of cardiac functions in the rats were determined by ultrasonic imaging with a high-resolution in vivo im-aging system.HE staining was used to observe the pathological changes.The protein expression of brain natriuretic peptide ( BNP) in the myocardium was assessed by Western blotting.The contents of BNP and cAMP in the serum and myocardium were measured by ELISA.The mRNA expression of basic fibroblast growth factor ( bFGF) and protein kinase A ( PKA) was detected by real-time PCR.RESULTS:Compared with blank control group, the myocardial cells in the model group had different degrees of necrosis and degeneration.Stroke volume and ejection fraction were decreased.The contents of cAMP and BNP in the serum and myocardium were increased in model group.The protein expression of BNP and the mR-NA expression of bFGF and PKA were also increased.CONCLUSION:Spleen-qi deficiency syndrome causes heart func-tion decline in rats.The expression of BNP, cAMP, PKA and bFGF is all increased.

Objective:To analyse the actual position of denture base and its relationship with the anatomic marks of alveolar ridge. Methods:According to the standardized process,10 sets of complete dentures were fabricated for edentulous patients by taking func-tional impression.Before and after completion of denture fabrication,trial wearing and full adjustment one week after application,the margin extension,thickness and form of the denture bases were measured.The relationship of denture base outline with anatomic marks of alveolar ridges was observed.Results:The most adjusted regions of upper and lower complete denture bases were hamular notch area,buccal shed area,far middle buccal corner region and retromylohyoid ridge area(P <0.05).Buccal outline of mandibular base was consistent with the external oblique ridge and the base edge corresponding to masseter muscle attachment in far middle buccal corner region was excluded from the base.Lingual outline was relieved in area of lingual frenum and was consistent with mandibular torus.Conclusion:Denture base is easily over extended in hamular notch area,buccal shed area,far middle buccal corner region and retromylohyoid ridge area.

Objective To explore the relationships among psychological empower, role stressors and job burnout in nurses.Methods From February to October of 2014, by random sampling method, 420 nurses from three hospitals of Jinzhou city of Liaoning province were selected and assessed with the psycho-logical empower questionnaires,the job burnout questionnaire,role stressors questionnaire.The data were ana-lyzed using pearson correlation analysis and logical multiple linear regression analysis.Results The average score of job burnout was (59.58±18.30) points, the average score of psychological empower was (38.87±7. 05) points.Job burnout and each dimension were negative correlated with self-determination of the psycho-logical empower ( r=-0.25--0.34, P<0.05) and self-efficacy ( r=-0.08--0.53, P<0.05).The scores of role stressors were positively correlated with the scores of emotional exhaustion( r=0.17-0.43, P<0.05) and cynicism ( r=0.07-0.46, P<0.05) .Multiple regression analysis showed that age(β=-0.104) ,education lev-el(β=-0.237),seniority(β=-0.334),self-determination(β=-0.152),self-efficacy(β=0.127),role am-biguity(β=0.284),role overload(β=0.325) were influencing factors of nursing burnout.Conclusion -Nurses role stressors and psychological empower can predict nurses’ burnout, so nurse managers can in-crease the level of authority to relieve psychological job burnout by reducing caregiver stress source.

Baby body plethysmography is a well-established technique of lung function determination,which is widely used to access the respiratory development and obstructive airway diseases by precisely measuring tidal breathing,functional residual capacity,and air way resistance.Babies who accept lung function measurements in the baby body plethysmography are under quiet state of spontaneous breathing without any subjective initiative,which results in multiplexed data acquisition,high precision and good repeatability.Clinical application of baby body plethysmography differs in different countries and areas,with the most intensive application in the German-speaking countries.In China,the clinical use of baby body plethysmography still remains in primary stage.Great progress has been achieved in its clinical use during the past 20 years,but there is still a lack of consensus on normal reference values or equations with which to interpret results,which has caused some trouble to diagnosis and identification of respiratory disease.In order to identify the nature and severity of airway disease,it is essential to understand the normal range of plethysmographic values that may occur in healthy infants of similar age,gender and body size.

Objective To investigate the effect of dopamine receptor in intestinal mucosal barrier function after brain injury in rats.Methods Twenty-four rats were allocated to control group,brain injury group,dopamine receptor group,and dopamine antagonist group according to the random number table,with 6 rats per group.Feeney' s weight-drop model was introduced to generate rat models of brain injury.Intestinal mucosal specimens were harvested at postoperative 7 days to evaluate intestinal mucosal morphology by HE staining,expressions of dopamine receptor D1 (DRD1) and dopamine receptor D2 (DRD2) by immunohistochemistry,and mRNA and protein expressions of DRD1 and DRD2 by real-time PCR and Western blot.Meantime,urinary samples were collected to measure lactulose to mannitol ratio (L/M) by high performance liquid chromatography (HPLC).Results Intestinal villus integrity was disrupted in brain injury group and dopamine receptor group when compared to control group,but it remained relatively intact in dopamine antagonist group.Ratio of L/M in brain injury group and dopamine receptor group was similar (0.192 ± 0.080 vs 0.183 ± 0.090,P ＞ 0.05),far higher than 0.037 ± 0.008 in control group (P ＜0.01),but it was reversed in dopamine antagonist group (0.071 ± 0.008,P ＜ 0.01).Real-time PCR showed DRD1 and DRD2 mRNAs expressed in brain injury group and dopamine receptor group were similar (0.764 ± 0.074 vs 0.718 ± 0.065,0.439 ± 0.051 vs 0.408 ± 0.090,P ＞ 0.05),far higher than 0.189 ± 0.008 and 0.076 ± 0.011 in control group (P ＜ 0.01),but both lowered in dopamine antagonist group (0.386 ± 0.071,0.270 ± 0.092,P ＜ 0.01).estern blot analysis showed DRD1 and DRD2 proteins in brain injury group and dopamine receptor group were similar,but both were far higher when compared to control group (P ＜ 0.01).Conclusion Impaired intestinal mucosal barrier may be relate to the up-regulated dopamine receptor in intestinal mucosa after brain injury in rats.

Objective To investigate the level of self-management behaviors and the quality of life with gastro esophageal reflux disease(GERD) patients,and analyze the correlation between self-management level and quality of life.Methods A total of 372 GERD patients were investigated by using Self-management Behavior Scale and Mos 36-item Short Form (SF-36) Health Survey.Results The level of self-management behaviors in GERD patients was relatively low.The total score of quality of life was (48.75±7.59) and the scores of the level of self-management behaviors was (5.13±0.59).Correlation analysis showed that there was positive correlation between quality of life and self-management behaviors(r=0.423,P＜0.01).Conclusion To promote the patients' health status,nursing staff should take measures to strengthen patient health education on the level of self-management behaviors for GERD patients.

[ABSTRACT]AIM:ToobservetheeffectofthesignalingpathwayofproteinkinaseC(PKC)-nuclearfactor-ery-throid 2-related factor 2 (Nrf2) on the expression of heme oxygenase-1 (HO-1) induced by cigarette smoke extract (CSE) in the rat airway epithelial cells .METHODS:After exposed to CSE , the airway epithelial cells of male SD rats were di-vided into control group , CSE3 group, RO318220 group, Nrf2 siRNA group and RO318220+Nrf2 siRNA group.The pro-tein levels of HO-1, Nrf2 and PKC were semi-quantified by Western blotting .The protein expression of HO-1 was assessed by immunocytochemistry .The mRNA expression of HO-1 was detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Nrf2 protein location was observed by immunofluorescence staining .HO-1 activity was also determined .RE-SULTS:After exposure to CSE for 3 h, the Nrf2 protein was mainly located in the nucleus , and the expression level of Nrf2 protein in the nucleus was stronger than that in the control cells .CSE also significantly enhanced the levels of p-PKC protein, and HO-1 mRNA, protein and activity .Pretreatment with RO318220 significantly decreased the levels of PKC pro-tein, Nrf2 protein, and HO-1 mRNA, protein and activity.Meanwhile, Nrf2 protein significantly decreased , and the activ-ity, mRNA and protein levels of HO-1 were also significantly attenuated by pretreatment with siRNA to knock down Nrf2. Pretreatment with RO318220 and Nrf2 siRNA significantly decreased the PKC protein , Nrf2 protein, and HO-1 mRNA, protein and activity .CONCLUSION:CSE up-regulates the HO-1 expression through PKC-induced nuclear translocation of Nrf2 in the rat airway epithelial cells .