Triple-negative breast cancer (TNBC) is a heterogeneous disease. Recently, the development of a gene expression profile fa-cilitated the re-classification of TNBC into six new subtypes, which show varied sensitivities to different therapies. In the era of preci-sion medicine, precision therapy may be directed at various potentially actionable molecular mutations in different subtypes of TNBC.

With the development of the next generation sequencing technology, considerable attention has been paid to the utility of circulating tumor DNA (ctDNA) detection in breast cancer. There are many clinical trials showed the ctDNA detection is a potential biomarker for the diagnosis, management and prognosis of breast cancer. ctDNA detection can provide a more accurate diagnosis for patients to guide clinical treatment in precision medicine era.

Objective To study the impact of head nurses'support on the creation of encouraging management atmosphere.Methods We randomly selected 120 clinical nurses from the Sir Run Run Shaw hospital.Then we adopted head nurses'support questionnaires,nurses'stressor questionnaires and career satisfaction questionnaires and drew argumentation of our hypothesis by introducing double-factor theory.Results The management atmosphere was only inferior to the workload among all of the stressor resources.The supportive behaviors of head nurses were positively related to the career satisfaction of nurses and the encouraging management atmosphere.Conclusion Head nurses'could relieve nurses'working pressure and unsatisfactory attitude toward work by using supportive management style.In this way they could increase encouraging factors in the management and facilitated the creation of encouraging management atmosphere and improvement of effective management.

Objective:To investigate the clinical efficacy and safety of montelukast combined with mometasone furoate in the treat-ment of children with allergic rhinitis. Methods:Totally 91 children with allergic rhinitis were randomly divided into the observation group (n=46) and the control group (n=45) according to the random number table. The control group was given mometasone fu-roate, while the observation group was treated with montelukast additionally. Both groups were treated for two weeks. The symptom scores including nasal congestion, sneezing, itchy nose and runny nose, serum level changes of IL-4, IL-12 and IgE and adverse drug reactions before and after the treatment in both groups were evaluated and compared. Results:The total effective rate in the observation group (93. 48%) was significantly higher than that in the control group (75. 56%, P<0. 05). Compared with those before the treat-ment, the symptom scores in both groups were decreased after the treatment (P<0. 05), and those in the observation group were lower than those in the control groups (P<0. 05). After the treatment, the serum levels of IL-4 and IgE in both groups were significantly de-creased when compared with those before the treatment, while the IL-12 levels were increased significantly (P<0. 05), and the chan-ges in the observation group were more significantl than those in the control group (P<0. 05). There were no serious adverse drug re-actions during the treatment course in both groups. Conclusion:Montelukast combined with mometasone furoate in the treatment of al-lergic rhinitis shows more notable efficacy with higher security.

Objective To discuss the application of ice-breaking action in the construction of nurse team, strengthen the cohesion of nurse team, therefore, to improve nursing quality. Methods First, let all nurses working in the same ward area to participate an action, namely ice-breaking action or secret angel action, in which every nurse chose secretly one of other nurses by drawing cards, then she often cared for that nurse secretly, namely she became a secret angel for that nurse. In return, she was also chosen and cared by one of others secretly. That means every nurse would become a secret angel, and owned a secret angel in the same time. Second, to test the change of happiness index of every nurse, the change of team's cohesion, and the change of patients' contentment indexes, before and after the secret-angel action was taken. Results Happiness indexes of 37 participants improved distinctly, and so did the contentment indexes of their patients after the secret-angel action was taken. Conclusions The secret-angel action can strengthen the cohesion of nurse team, improve happiness indexes of nurses, contentment indexes of their patients, and improve nursing quality.

Objective To investigate the expression of PES1 in ovarian cancer and its relationship with the vascular endothelial growth factor(VEGF) expression .Methods The expression of PES1 in ovarian cancer tissues and corresponding pericancerous tis-sues was detected with Western blot .Results The expression of PES1 in ovarian cancer tissues was obviously higher than that in the pericancerous tissues .The secretion amounts of VEGF in cell culture fluid of CAOV-3 and ES-2 with transfection of FLAG-PES1 were elevated from (178 .0 ± 11 .8) pg/mL and (309 .5 ± 18 .5) pg/mL to (375 .0 ± 18 .3) pg/mL and (633 .2 ± 25 .7)pg/mL respectively (P<0 .01) ,the secretion amounts of VEGF were also increased(P<0 .01) .The relative VEGF mRNA levels were also raised by 1 .8 times and 2 times respectively (P< 0 .01);Western blot simultaneously showed that the over expression of PES1 could increase the expression of HIF-1αin these two kinds of cells .The luciferase report gene transcriptional activation activity de-tection reveald that PES1 had no direct effect on the VEGF transcription .After cotransfecting HIF-1αSiRNA and FLAG-PES1 to CAOV-3 and ES-2 cells ,Western blot demonstrated that HIF-1αSiRNA could obviously inhibit the increase of HIF-1αexpression induced by PES1 ,at the same time the secretion amounts of VEGF and mRNA level were also supressed .Conclusion The expres-sion of PES1 is obviously up-regulated in ovarian cancer tissues .

AIM: The multiple effective components in Yiqing Capsule(Radix et Rhizoma Rhei,Rhizoma Coptidis,Radix Scutellariae) were analyzed by RP-HPLC. METHODS: A column of Kromasil C_(18)(250 mm?4.6 mm,5 ?m)was used.The mobile phase was composed of A 0.5% triethylamine(pH3.0,containing 5 mmol/L SDS),B methanol and acetonitrile(50∶50) with gradient elution.The flow rate was 1.0 mL/min with the detection wavelength at 254 nm. RESULTS: In the linear range the correlation coefficients ranged from 0.999 4 to(0.999 9).The average recoveries were 95.2%-102.2% with corresponding RSD of 0.8%-2.0%,respectively. CONCLUSION: The method is rapid,accurate and reliable.It can be used to control the quality of Yiqing Capsule.

Objective To investigate effect of leptin on nerve function in patient with Parkinson disease .Methods 68 cases patients with PD were selected and randomly divided into two groups, according to random number table, from October 2013 to December 2014 in the hospital.34 cases in control group were treated with conventional therapy, and 34 cases in experimental group were treated with leptin, with a consecutive treatment of twelve weeks.The score of activity of daily living scale ( ADL ) and mini-mental state examination ( MMSE ) were analyzed pre and post-treatment. ResuIts After treatment, the ADL score of experimental group was significantly lower than that of control group ( P<0.05 ) , MMSE score of experimental group was significantly higher than that of control group (P<0.05).The adverse reactions in both groups were minor.ConcIusion Leptin has good curative effect in treatment of Parkinson disease, and could significantly improve the neurological function of patients, with mild adverse reactions.

AIM:To establish a method for determining menthol, methyl salicylate,cinnamal dehyde and engenol simultaneously in Baicao Oil by GC. METHODS: A separation was performed on a large bore capillary column(30m?0.53mm) with PEG-20M as the stationary phase of GC.The method was carried out with FID as the detector without split stream sampling.A programmed temperature was employed.Benzyl alcohol was selected as the internal standard substance. RESULTS: An excellent seperation of menthol,methyl salicylate,cinnamal and engenol in Baicao Oil was gained.The good linear relationships of menthol (62.7?10~3-2.01?10~3 ?g/mL),methyl salicylate (119?10~3-3.81?10~3 ?g/mL),cinnamal dehyde (0.42-205 ?g/mL),engenol (19.9-636 ?g/mL) were observed.All correlation coefficients were greater than (0.999 2).The average recovery of menthol,methyl salicylate,cinnamal and engenol were between(98.6%)～100.3%,and RSD was less than 2.4%. CONCLUSION: The method is simple,accurate and suitable for quality control of Baicao Oil.

Colorectal Neoplasms ; pathology ; surgery ; Humans ; Liver Neoplasms ; secondary ; surgery ; Neck Dissection