Objective To reduce information loss in the refining processing of photoplethysmographic (PPG) pulse waveform. Method A novel PPG pulse waveform refining processing technique, named truncation/extrapolation technique was proposed. The key point of the proposed technique was to preserve the shape pattern of the most significant parts of the waveform at the expense of the distortion of the insignificant part. Thus, as compared with the conventional time-normalization method, the proposed technique ensured the accumulation coherent of the signal energy could be. Result Waveform analysis and comparison of experimental data between two methods verified the effectiveness of the proposed method in maintaining the most significant time domain features of the pulse waveform over a relatively wide range of the heart rate change, especially the shape and width of the dominant peak and the position of the dicrotic notch of the first derivative of PPG(dPPG). Conclusion The proposed method reduces the information loss in PPG pulse waveform refining processing, thus helps further processing of the PPG signal using some advanced signal processing techniques, such as waveform modeling and pattern recognition.

Objective To study the effects of phase change material (cooling agent) on the quality of blood after long-distant transportation.Methods A total of 12 bags, packed with pre-chilled phase change materials, were put into regular blood transportation kit, and the new blood transportation kit, which was developed by our hospital. In each kit there were 54 U of red blood cell suspensions. The temperatures in the kit were recorded during the transportation at different times.Results The temperature in the kit was kept between 1—7℃ during the 72h transportation. The temperature met the needs of red blood cell transportation.Conclusion Phase change material could be used as an effective cooling agent for long distance blood transportation.

Cryoprecipitation is one of the most important blood components.With the deeper study about cryoprecipitation,it has been used in clinics more and more extensively.This articles reviewed the new development of cryoprecipitation in clinical uses.

Objective To explore the effects of ultraviolet irradiation on platelet. Methods Three assays were been developed.These include platelet count,release of alpha granule membrane protein 140(GMP-140)and morphology of platelet by electron micrograph in those samples before and after ultraviolet irradiation.Results Compared with the nonirradiated controls,the irradiated units showed significant changes including decrease of platelet count,increase of release of GMP-140 and platelet lesion in morphology.Conclusion Ultraviolet irradiation may have activation and lesion effect in platelet.

Objective To test the performance of field blood transportation vehicle from the aspects of heat preservation,refrigeration and oil consumption.Methods Two vehicles without power supply were stowed with maximum load,one with red cell suspension,and the other with frozen plasma.With the environmental temperature higher than 35 ℃ or lower than 25 ℃,related time lengths were detected respectively.Results The holding time of the vehicles was from 1.5 h to 7 h,refrigerating time was from 15 min to 138 min,and a full tank of gasoline was exhausted around 5～7 hours.Conclusion During line-haul,field blood transportation vehicle has to be equipped with additional cooling agents,in case the refrigeration equipment out of work for the reason of gasoline exhaustion.

Objective To investigate the relationship between serum 1 ,25‐hydroxy vitamin D3 concentration level and children with bronchial asthma .Methods 222 children with bronchial asthma treated in paediatrics of Zhongshan Boai hospital from 2012 Jan 2014 Dec were chosen as asthma group(A) ,while 223 cases of healthy children were selected as control group(C) .Electro‐chemical luminescence method was used to detect the serum level ,and its effect on asthma was disscussed .Results The concen‐tration of serum 1 ,25‐hydroxy vitamin D3 of asthma group (86 .30 ± 27 .70 nmol/L) was low than the control group ( 93 .39 ± 27 . 15nmol/)L .Serum levels of asthma group in different age groups (A1 ,A2 and A3) were 106 .87 ± 28 .28 nmol/L ,88 .98 ± 20 .65 nmol/L ,68 .38 ± 19 .23 nmol/L respectively ,While those of control groups were (110 .00 ± 22 .30) nmol/L ,(87 .36 ± 24 .42) nmol/L ,(21 .23 ± 16 .42) nmol/L respectively .Male and female serum level in asthma group were (88 .26 ± 27 .73)nmol/L and (81 .94 ± 26 .91)nmol/L respectively .While those of control group were(91 .91 ± 26 .57)nmol/L and (85 .69 ± 28 .04) nmol/L re‐spectively .Conclusion There is significant correlation between serum 1 ,25‐hydroxy vitamin D3 level and children with bronchial asthma .,yet no correlation between serum level and gender .1 ,25‐hydroxy vitamin D3 duficiency is one of the risk factors of chil‐dren asthma .

Objective: To investigate the temperature control methods for long-distance transportation of red blood cell suspensions in military affairs or emergence.Methods: We loaded blood transport cars with red blood cell suspensions to full capacity and kept the temperature by combined cooling methods.Results: The temperature in the container was kept between 2-6.2℃ during the 72 h transportation,even when the refrigeration system of the car was out of work.Conclusion: The required temperature for long-distance transportation of red blood cell suspensions could be attained by combined cooling methods in case of insecurity of transportation time and the effect of single cooling method.

Background and purpose: Multidrug resistance (MDR) of tumor cell is the main reason for clinical chemotherapy failure as well as cancer recurrence and metastasis. This study was to construct a lentiveral vector of RNA interference of MDR1 gene and observe its inhibitive role on the expression of MDR1 in A549 and A549/DDP cells. Methods: Oligos of base pairs for hairpin RNA targeting MDR1 were chemically synthesized. Via annealing and inserting them into the down-stream of H1 promoter of linearized pSUPER, we obtained the siRNA constructs for MDR1, which were afterwards transfected into A549, a human lung cancer cell line expressing high level MDR1, the impact of constructs was observed on the expression and interference efficiency of siRNA against MDR1. The effective sequence of siRNA targeting MDR1 gene was confirmed. Both sense and antisence oligo DNA of the targeting sequence was designed, synthesized and cloned into the PTM vector, containing a promoter and a green fluorescent protein (GFP). The resulting lentiviral vector containing MDR1 siRNA was named PTM-siMDR1 and then transfected into A549 and A549/DDP cells after being confirmed by PCR and sequencing. Results: Restriction digestion and DNA sequencing showed that the siRNA constructs for MDR1 were successfully produced and the expressed siRNA could effectively down-regnlate the expression of MDRI. PCR demonstrated that the lentivirus RNAi vector of MDR1 producing PTM-siMDR1 was constructed successfully. The chemosensitivity of A549/DDP cells to cisplatin were enhanced obviously after trartsfection. Conclusion: The lentivirus RNAi vector of MDR1 can significantly revise the resistance ofA549/ DDP cells with eisplatin after infection.

Objective To investigate the clinical efficacy of Dahuang Xiaozhi Suppository combined with tetracycline in treating type Ⅲ prostatitis infected with nano-bacteria. Methods Totally 120 patients of type Ⅲprostatitis infected with nano-bacteria were randomly divided into treatment group and control group, with 60 cases in each group. Both groups were disabled anti-infective drugs and other preparations for diet and life intervention. Both groups received tetracycline, once a tablet, twice a day, orally. The treatment group received Dahuang Xiaozhi Suppository, once a capsule, once a day, placing in the anus 3-4 cm. 10 d was a treatment course, for 3 courses. The clinical efficacy, major symptoms improving time, NIH-CPSI, leukocyte count and ecithin corpuscles in expressed prostatic secretion (EPS), urinary flow rate, and cytokine content of TNF-α, IL-6, IL-8, were evaluated. Results The total effective rate was 100.0% (60/60) in treatment group and 83.3% (50/60) in the control group, and treatment group was higher than the control group (P<0.05). Pelvic pain, urinary symptom, and scrotum wet improvement time of treatment group were lower than the control group (P<0.05). Compared with before the treatment, the NIH-CPSI pain scores, urinary symptom scores, life quality score, leukocyte count and TNF-α, IL-6, IL-8 content of EPS in both groups were significantly lower after treatment (P<0.05), and the cases with lecithin corpuscles +++ - ++++ in both groups significantly increased after treatment (P<0.05). The urinary peak flow rate and mean flow rate in both groups were higher after treatment (P<0.05). There was statistical significance in the scores of NIH-CPSI pain, urinary symptom and life quality, leukocyte count and TNF-α, IL-6, IL-8 content of EPS, and the cases with lecithin corpuscles ++++ between the two groups (P<0.05). Conclusion Dahuang Xiaozhi Suppository can improve the efficacy of treating type Ⅲ prostatitis infected with nano-bacteria. Combining Dahuang Xiaozhi Suppository with tetracycline can reduce prostate inflammation, with obvious efficacy.

This study examined the association of pregnancy with urolithiasis and provided new insights into urolithiasis in pregnancy. A total of 462 subjects were studied from January 2004 to December 2009 in Foshan Maternal and Child Health Hospital, China. Among the 462 subjects, 162 cases of urolithiasis during pregnancy (UPG) were selected as the observation group, 150 cases of no urolithiasis during pregnancy (NUPG) served as pregnancy control group, and 150 cases of no pregnancy (NPG) at reproductive age who took part in physical examination were randomly assigned into non-pregnant control group. At the same time, the patients in observation group were divided into the following sub-groups: no symptomatic urinary calculus (NSUC) and symptomatic urinary calculus (SUC) groups; SUC group was further divided into surgical intervention (SI) and conservative management (CM) groups. The general information and the data of blood and urine were collected and compared among the groups. The results showed that the incidence of urinary calculi in pregnant women was lower than that in non-pregnant women, the formation of urinary stone was associated with the change of metabolism of protein and sugar in pregnant women, and the surgical intervention was a practicable alternative to treat the clinical intractable symptomatic urinary calculi in pregnancy.