Objective To explore cardiovascular risk factors in patients of hypertension (HTN) complicated with non-alcoholic fatty liver but normal aminotransferase. Methods Forty-four cases (control group) of uncomplicated hypertension and 56 cases (study group) of hypertension complicated with nonalcoholic fatty liver but normal aminotransferase were screened from those who visited physical examination center of the hospital for regular checks-up during October 2009 to June 2010. Blood pressure ( BP), body mass index ( BMI), fasting plasma glucose (FPG), total serum cholesterol (TC) , triglyceride (TG), highdensity lipoprotein-cholesterol ( HDL-C ), low-density lipoprotein-cholesterol ( LDL-C ), gammaglutamyltransferase ( GGT ), uric acid ( UA ) , highly-sensitive C-reactive protein ( hs-CRP) and homocysteine (Hey) of the two groups were compared. Results Comparing with the control group, the study group had higher diastolic blood pressure ( DBP) (t = 1898. 5, P < 0. 05) , BMI (t = - 5. 036, P < 0.01), FPG (t= -2.026, P<0.05), TG (t = 1923. 5, P<0.05), GGT (t = 1789, P <0.01), UA 0 = 1715, P<0.01), hs-CRP (t = 1832.5, P < 0.01) and lower level of HDL-C (t =2357.0, P< 0. 01). Serum activity of GGT correlated positively with BMI (rs = 0. 349, P = 0. 000), TG ( rs = 0.413,P =0.000), UA (rs =0.446, P= 0.000) and hs-CRP (rs =0.350, P = 0.000), respectively.Conclusions When patients of hypertension complicated with non-alcoholic fatty liver but normal aminotransferase, their cardiovascular risk factors clustered further, with serum activity of GGT obviously increased which correlated positively with various risk factors, indicating increase in cardiovascular risks.

OBJECTIVE:To observe therapeutic efficacy of ursodeoxycholic acid(UDCA)combined with S-adenosyl methio-nine (SAMe) in the treatment of mild intrahepatic cholestasis (ICP) of pregnancy. METHODS:213 pregnant patients with mild ICP were selected and randomly divided into combination group (107 cases) and single drug group (106 cases). Combination group received UDCA combined with SAMe,and single drug group was given UDCA alone. Therapeutic efficacy and pregnancy outcome were compared between 2 groups. RESULTS:Compared to before treatment,TBA,CG,DBIL,ALT and AST of 2 groups were decreased after treatment,and the decrease of combination group was more significant than that of single drug group, with statistical significance(P<0.05). The time of itching control [(3.8±1.4)d] in combination group was lower than(5.3±1.5) d in single drug group,with statistical significance (P<0.05). After 3 and 5 days of treatment,itching degree score of combina-tion group was lower than that of single drug group,with statistical significance(P<0.05). There was no statistical significance in fetal distress rate,neonatal asphyxia rate,amniotic fluid contamination rate,cesarean section rate and neonatal 1 min Apgar score between combination group and single drug group(P>0.05);the preterm birth rate of combination group was 11.22%,which was significantly lower than 20.75% of single drug group,with statistical significance(P<0.05). CONCLUSIONS:Compared to UD-CA alone,UDCA combined with SAMe in the treatment of patients with ICP can control the clinical symptoms timely,recovery laboratory index as soon as possible and obtain better therapeutic efficacy.

Objective To determine the aberrant methylation status of TFPI-2,FOXE1 and NPTX2 gene promoter region in bile from cholangiocarcinoma patients and the diagnostic value for cholangiocarcinoma. Methods Methylation—specific PCR(MSP)was used to detect the promoter methylation status of TFPI-2, FOXE1 and NPTX2 genes in bile from 29 patients with cholangiocarcinoma and 27 patients with choledocholi-thiasis.The sensitivity and specificity of combined methylation of 3 promotors and brush cytology were analyzed. Results There was significant difference between the methylation rate of NPTX2,FOXE1 in choledocholithiasis group and cholangiocarcinoma group(75. 86% VS 25. 93%,χ2 = 13. 964,P<0. 05;44. 82%VS 14. 81%,χ2 = 4. 622,P<0. 05).There was no significant difference between the methylation rate of TFPI-2 in choledocholithiasis group and cholangiocarcinoma group(17. 24%VS 18. 52%,χ2 = 0. 016,P>0. 05).Positive rate was 34. 48% with endoscopic retrograde brush cytolog,and 86. 20% with promoter methylation of FOXE1 combined with NPTX2 genes,and there was significance difference in the positive rates between the two methods(χ2 = 14. 122,P< 0. 05). Conclusion The methylation rates of NPTX2,FOXE1 were significantly higher in choledocholithiasis group than those in the cholangiocarcinoma group. Detection of comethylation rate of the FOXE1 and NPTX2 genes could improve diagnostic sensitivity of cholangiocarcinoma.

OBJECTIVE:To observe therapeutic efficacy and safety of tobramycin dexamethasone in the treatment of meibo-mian gland dysfunction(MGD). METHODS:108 MGD patients were randomly divided into control group(53 cases)and observa-tion group(55 cases). Control group was given eyelid cleaning with conventional physical cleaning method,at the same time,giv-en Doxycycline hydrochloride tablets 0.1 g orally after meal,twice a day. Based on control group,observation group was addition-ally given Tobramycin and dexamethasone eye ointment with length of 1.0-1.5 cm,smearing into conjunctival sac,4 times a day. Treatment courses of 2 groups lasted for 4 weeks. Clinical efficacies of 2 groups were observed as well as eyelid observation score, symptom score,the levels of IL-6 and TNF-α,the occurrence of ADR before and after treatment. RESULTS:The total response rate of observation group was significantly higher than that of control group(89.1% vs. 77.4%),with statistical significance(P<0.05). Before treatment,there was no statistical significance in eyelid observation score,symptom score,the levels of IL-6 and TNF-α between 2 groups(P>0.05). After treatment,eyelid observation score of 2 groups were significantly higher than before treatment,and the observation group was significantly higher than the control group;symptom score and the levels of IL-6 and TNF-α in 2 groups were significantly lower than before treatment,and the observation group was significantly lower than the con-trol group,with statistical significance(P<0.05).There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Based on routine treatment,tobramycin dexamethasone shows significant therapeutic efficacy for MGD,and can improve clinical symptoms significantly but does not increase the occurrence of ADR.

N-acetyltransferase are phase II code proteins with overlapping substrate specifici-DMEs, and two highly similar human NAT gene ty. NAT2 is a polymorphic acetyltransferase gene (designated NAT1 and NAT2) are shown to en- locus and "slow acetylation" in humans is due tonutations in the single coding exon of the NAT2 gene. It was also demonstrated that there exist discrete NAT1 structural variants, and differences in tissue levels of NAT1 among humans are related to specific sequence differences in the NAT1 structural gene. Biochemical studies have shown that NAT1 and NAT2 play an important role in the metabolism of some carcinogens, epidemiological studies have revealed an association between ploy-morphisms of NATs and increased cancer risk.Metabolic phenotypes/genotypes can significantly influence DAN adduct formation and could ultimately lead to alterations in cancer risk. If unequivocal biomarkers of genetic susceptibility to cancer can be developed successfully, then identification of individuals at increased risk would be very helpful in the fields of public health and preventive medicine.

Objective To investigate the influence of Hui medicine flatiron moxibustion therapy and nursing intervention on pain and serum IL-2,TNF-α in rheumatoid arthritis (RA) patients before and after treatment and care interventions.Methods 89 cases RA hospitalized patients from Traditional Chinese Orthopedics and Traumatology department of General Hospital of Ningxia Medical University were collected and were randomly divided into the control group (43 cases) and the treatment group (46 cases) from May 2011 to September 2013.Two groups used a combination method of overall and portion acupoints.The control group used warm acupuncture,30 min every time,1 time per day,routine care was given.The treatment group was given with Hui medicine flatiron moxibustion instrument by the professional Chinese medicine nurses,direct moxibustion acupoints site to portion skin slight degree redness,30 min every time,1 time per day,and with routine care,focused on the reasons things out the enlighten,emotion,suggested transferring,calm the nerves of the traditional Chinese medicine psychological care guidance.10 times as a course,to rest for 3～5 days on treatment interval,for efficacy evaluation after three courses.Meanwhile,before and after the application,to test the pain degree with NPRS; to assess the psychological and emotional status with SCL-90,serum IL-2 and TNF-α levels were tested with radioimmunoassay.Results The total effective rate was 89.13％ on the treatment group,the control group was 74.42％.After treatment,the NPRS,SCL-90 scores were significantly improved than those before treatment; the NPRS,SCL-90 scores of the treatment group were better than those of the control group.After treatment,serum IL-2 and TNF-α levels were significantly improved compared with those before treatment; after treatment,IL-2 and TNF-α levels of the treatment group were better than those of the control group.All the above comparison had statistical significance.Conclusions Hui medicine flatiron moxibustion therapy and nursing intervention may effectively improve pain in RA patients,ease the psychological anxiety,increase serum IL-2,TNF-α levels,enhance the quality of life for patients,and promote disease recovery.

Objective To quantize the synergistic force in movement of upper limbs between shoulders and elbows. Methods The trans-verse forces of elbows and shoulders during movement were recorded in a healthy adult with an upper-limb-force-measuring plate form which comprised of 2 three-dimensional force sensors, respectively. Then he performed shoulder abduction/adduction and elbow extension/flexion at 100%, 75%, 50%and 25%of the maximum contraction force, respectively. The ratio of the active action force and the joint action force (named assessment index) was used to assess the synergistic degree of the forearm and the upper arm. Results In the shoulder abduc-tion motion, the assessment index decreased as the strength of active action decreased, meant interference of joint action increased. Howev-er, it was almost stable in the shoulder adduction, increased in the elbow extensionas, and was irregular in the elbow flexion, as the active ac-tion strength decreased, respectively. Conclusion It may be more difficult to control upper arm than the forearm.

The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminePLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90% - 95% and it had no obvious attenuation within 84 h. However, the plasmid transfection rate was only 5% -25% and it was decreased significantly within 60 h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.
Green Fluorescent Proteins/genetics ; HL-60 Cells ; Liposomes ; Oligonucleotides/*genetics ; Plasmids/*genetics ; Transfection

Objective To evaluate the control effect of comprehensive measures of schistosomiasis after its transmission in?terruption in Kaihua County,Zhejiang Province,so as to provide the references for further consolidation of schistosomiasis con?trol. Methods The data of Oncomelania hupensis snail survey and control,as well as environmental reform in Kaihua County were collected and analyzed from 1996 to 2015. Results From 1996 to 2015,totally 2 635 snail habitats and 102.75 hm2 area with snails were found,and 125.4 thousand snails were dissected and no one was schistosome infected. The accumulated snail control area was 4 932.98 hm2,and the area with snails was effectively reduced by the comprehensive control measures. Conclu?sion The schistosomiasis control effect could be consolidated by the comprehensive control measures emphasizing environmen?tal reconstruction,and the snail surveillance work still should be strengthened.

Background Measurement of corneal thickness is important for the early diagnosis and treatment of some eye disorders,including corneal diseases and refractive errors.However,the corneal parameters from schoolage population are rarely reported.Objective The aim of this survey was to characterize the central corneal thickness (CCT),minimum corneal thickness (MCT) and paracentral corneal thickness in healthy Chinese schoolage population.Methods A cross-sectional study was designed in this study.Children aged 7 to 15 years with the diopter of-3.00 D to +3.00 D were recruited from two primary schools in Baoshan district in Shanghai based on random cluster sampling under the approval of Shanghai First People's Hospital and informed consent of child custodian.Routine examinations were firstly performed to determine the healthy participants.CCT (within 2 mm range away the corneal vertex),MCT and paracentral corneal thicknesses (2 to 5 mm zone away the cornea vertex in superior,inferior,nasal and temporal quadrants) were then measured by RTVue Fourier optical coherence tomography (OCT) for the comparison between both eyes and different gender.The subjects were grouped into the 7-9,10-12 and 13-15 years groups,and the correlations between age and CCT,MCT and paracental corneal thicknesses were analyzed.The coordinate position of the thinnest cornea was determined.Results A total of 147 children were enrolled in the study.The mean CCT value of the right eyes was (537.77±29.33) μm,and that of the left eyes was (539.22±29.16) μm,showing a significant difference between them (t =-3.21,P =0.00).The paracentral corneal thicknesses of the right and left eyes were (565.52±30.11) μm and (568.42±31.07) pm in the superior quadrant,and those in the temporal quadrant were (549.01 ±30.46) μm and (547.24±30.23) μm,with significant differences between them (t =-2.47,P =0.01 ; t =2.12,P =0.04).No significant difference was found in the CCT,MCT,paracentral corneal thicknesses from various quadrants (all at P＞0.05).In addition,no considerably correlation was seen between age and CCT,MCT and paracentral corneal thickness (all at P＞0.05).The thinnest cornea area was located in the inferotemporal region in 40.82％ right eyes and 57.82％ left eyes.The distance of thinnest cornea area away corneal vertex was (0.62±0.33)mm in the right eyes and (0.91±0.63)mm in the left eyes,with a significant difference between them (t =-5.17,P =0.00).Conclusions The central,superior and temporal corneal thicknesses are significantly different between the right and the left eyes among healthy Chinese school-age children,but corneal thickness change is not associated with age or gender.The thinnest corneal area does not locate at the vertex.