Description of the security grading protection used in the security protection system for information systems in medical organizations. Elaboration of the research ideas, process and some outcomes for the Fundamental Requirements for Security Grade Protection of Information Systems in Medical Organizations, from the five aspects of system modeling, grading guidance for industries, threat and risk analysis, security objective output, and security adjustment.

Objective To evaluation of curative effect of Yiqi-Wenshen decoction combined with salmon calcitonin subcutaneous injection for the patients with postoperative bone atrophy after calcaneal fracture.Methods According to the inclusion criteria, 120 patients with calcaneal fracture were randomly divided into 2 groups, 60 in each group. The control group received the subcutaneous injection of salmon calcitonin, and the combined group received the control group treatment plus Yiqi-Wenshen decoction. Both groups were treated for 6 weeks. The X-ray was used to detecte the local bone density of limbs, and the foot function scores of ankle function and the changes of clinical symptoms and signs before and after treatment were observed.Results After treatment, joint pain (2.8 ± 1.1vs. 3.6 ± 1.1,t=4.129), tenderness (1.0 ± 0.5vs. 1.4 ± 0.7,t=3.297), the color change of (1.5 ± 0.8vs. 2.0 ± 0.6,t=3.117) score in the combined group were significantly lower than those in the control group (P<0.05). The degree of swelling (112.5 ± 11.8 cm3vs. 122.5 ± 13.6 cm3,t=3.816) in the combined group was significantly lower than that in control group (P<0.05). The bone mineral density (0.762 ± 0.020 g/cm2vs. 0.722 ± 0.023 g/cm2,t=3.803,P<0.05) in the combined group was significantly higher than that in the control group. The effect rate of the combined group was 90% (54/60), and the control group was 70%. There was significant difference between 2 groups (χ2=7.500,P=0.006). The effect rate of ankle joint function of the combined group was 83.3% (50/60), and the control group was 70% (42/60). There was significant difference between 2 groups (χ2=4.444,P=0.035).Conclusions The Yiqi-Wenshen decoction combined with subcutaneous injection of salmon calcitonin could relieve the pain, swelling and other symptoms of the patients with bone atrophy after calcaneal fractures, and could benefit the recovery of ankle joint function.

Objective To investigate whether 5, 7-dihydrox-8-nitrochrysin (NOC) induces apoptosis in U937 monocytic leukae-mia cells is involved in the regulation of the activity of PKD 2.Methods U937 cell line cells were cultured in vitro .Apoptosis rate was analyzed by flow cytometry (FCM) using propidium iodide (PI) staining.DNA ladder bands were observed by DNA agarose gel electro-phoresis.The phosphorylated protein expression of PKD 2 was analyzed using Western blot .Results NOC (2.5, 5.0, and 10.0μmol/L) increased apoptosis rate in U937 cells in a concentration-dependent manner ( P <0.05).After treatment with NOC (5.0 and 10.0μmol/L) for 24 h, U937 cells presented typical DNA ladder bands .At the same time, not only did NOC effectively down-regulate the ex-pression of PDK2 phosphorylated protein , but also increased apoptosis rate in U 937 cells in the presence of G?6976, a specific inhibitor of PKD2.Conclusions The effect that NOC induces apoptosis in U 937 cells is related to the inhibition of the activity of PKD 2.

BACKGROUND: In the research of biomaterial, especially bio-coatings field, how to find a good biological and mechanical properties basal body, and to prepare coatings with uniform and compact surface and strong bonding force, is the key factor in clinical Application of artificial prosthesis. OBJECTIVE: To review the progress of bio-coatings.METHODS: Databases of CNKI, VIP, and Science Direct were retrieved with key words of "Biocoatings, HAp, Titanium, Carbon/carbon composites" to search papers published from January 1998 to October 2009. PApers underlying bio-coatings were selected. Totally 216 literatures were initial searched, according to inclusive criteria, 31 literature were summarized. RESULTS AND CONCLUSION: By contrasting the basal bodies made of titanium or carbon/carbon and different coating preparation, we found that the basal body composed of carbon/carbon composites exhibited good biocompatibility, which had similar strength to natural bone. Using hydrothermal method, the composites coating prepared by taking carbon/carbon as basal body and hydroxyApatite, chitosan, or polymethyl methacrylate as coatings provided a new idea for increasing the combing strength between coatings and basal body.

Background and purpose:Ovarian cancer is associated with a high recurrence and mortality due to the existence of cancer stem cells. The purpose of this study was to investigate the effect of casticin (CAS) on the capability of self-renewal in ovarian cancer stem cell like cells (OCSLCs) derived from SKOV3 cell line. Methods:SKOV3 cell line cells were cultured in vitro, and OCSLCs were obtained and amplified through suspended culture with conditioned medium of the stem cells. The phosphorylation level of FoxO3a was analyzed using Western blot. The protein expression of FoxO3a was inhibited by FoxO3a speciifc siRNA transfection, and then the ratio of sphere-formation was detected. Results: Compared with parental cells, OCSLCs over-expressed phosphorylated FoxO3a (pFoxO3a) and had elevated ratio of sphere-formation [(3.1±0.3)% vs (34.8±6.8)%, P<0.05]. CAS significantly inhibited the capability of sphere-formation in OCSLCs and down-regulated the expression level of pFoxO3a. And the transfection of FoxO3a speciifc siRNA suppressed the protein expression of FoxO3a and attenuated the inhibitory effect of CAS on the sphere-formation of OCSLCs. Conclusion: Reduced expression level of pFoxO3a is involved in the effect that CAS inhibits sphere-formation of OCSLCs derived from SKOV3 cell line.

Since the hospital metrology station is the principal part of military metrology system,its handwrought operation greatly affects the working efficiency and implementation effects.In order to enhance working automatization,some metrology software was developed,but they only focused on the metrology management.This software achieves more functions such as auto-hint,auto-count of the inspection parameters,auto-output of the certification and so on.

AIM:To investigate the effects and mechanism of Le Er Mai(LEM) on the apoptosis of hippocampus neuronal cells in the anaphase of cerebral ischemic reperfusion injury in rats.METHODS:A rat model of middle cerebral artery occlusion reperfusion(MCAO) was produced with the intraluminal filament.During reperfusion for 30 d after 2 h of ischemia,the TUNEL staining methods were used to detect apoptosis of hippocampus neuronal cells,and immunohistochemical technique were employed to examine the protein expression of Fas,Bax,caspase-3 and caspase-9 in the hippocampial.The gene expressions of fas,bax,caspase-3 and caspase-9 in hippocampial were examined by RT-PCR.RESULTS:After 2 h ischemia and 30 d reperfusion,compared with sham-operated group,TUNEL-positive staining cells and expression levels of Fas,Bax as well as caspase-3 and caspase-9 obviously increased,and the mRNA expressions of fas,bax,caspase-3 and caspase-9 in hippocampial markedly up-regulated in model group.Compared with model group,LEM at dose of 2.00 g/kg or 0.87 g/kg,and flunarizinum significantly reduced apoptosis and decreased the protein expressions of Fas,Bax,caspase-3 and caspase-9 in hippocampial,and down-regulated the mRNA expressions of fas,bax,caspase-3 and caspase-9(P

Objective To explore whether Forkhead O transcription factor-3a (FoxO3a) activity affects the capability of sphere-formation of ovarian cancer SKOV3 cell line.Methods Sphere-forming cells (SFCs) were obtained and amplified through suspended culture with conditioned medium of the stem cells in SKOV3 cell line.After SKOV3 cells were transfected with FoxO3a specific siRNA,the protein expressions of FoxO3a and Bmi1 and the ratio of sphere-formation were compared with Western blot and sphere-forming assay,respectively.Results Compared to parental cells,SFCs from SKOV3 cell line had higher ratio of sphere-formation and over-expressed Bmi1 and pFoxO3a.Transfection of FoxO3a specific siRNA down-regulated the protein expression of FoxO3a and upregulated expression of Bmi1 in SKOV3 cells,and enhanced the capability of sphere-formation.Conclusions Silence of FoxO3a leads to enhanced capability of sphere-formation in SKOV3 cell line.

OBJECTIVE:To investigate the protective effect of Guishu blood-activating capsule on cerebral ischemical reperfusion injury of rats.METHODS:The cerebral embolism model rats were established,the rats were randomly divided into model group,nimodipine positive control group,Guishu blood-activating capsule(high,medium and low dosage groups,re?spectively)group and with another sham operation group established.Each group was administered intragastrically with water or the corresponding drugs,the cortexes and hippocampus tissues of the rats were divided into the injured side and the control side,the contents of aspartate(Asp),glutamate(Glu),malonaldehyde(MDA),lactate dehydrogenase(LD),the tumor necrosis factor?(TNF-?)and the activity of glutathione peroxidase(GSH-Px)were determined,the cerebral infarct size was determined by red tetrazoline staining method and the cerebral water content was determined by oven dry process.RE?SULTS:Compared with the model group,in one or several sub-groups of Guishu group,no marked change was noted in the content of Asp while that of the Glu in the injured side of the hippocampus tissues decreased significantly(P

Objective To study the relationship between the selection of tube tension in digitalchest radiograph and image quality.Methods When tube current was fixed at 4 mAs, the choice of X-ray tube voltage changed from 60 to 120 kV.CDRAD2.0 contrast details phantom and normal human chest were exposed by X-ray system with 7 kinds of tube voltage (the difference between tube voltage was 10 kV) ,and the X-ray incidental dose of phantom surface was measured.Five radiologists with 3 years working experience evaluated the image quality on monitor and calculated the image quality factor (IQF) and image reading score.Statistics analysis was then performed by using ANOVA test and t test.According to the results, the optimum tube voltage range was determined.Results (1) The incidental dose of phantom surface increased with the higher tube voltage.(2)When the tube voltage was changed from 60 kV to 120 kV, the IQF value observed in CDRAD2.0 phantom image on monitor was 75.0±10.4,57.1±6.4,52.7±2.5,47.9±4.5, 46.0±3.8,46.0±2.8,45.2±3.5 ,there was significant statistical differences between groups(F=19.10, P<0.01).(3)The integrated score of the chest image quality in the tube voltage 90 kV and 120 kV were 12.4±0.9 and 13.0±0.7, respectively, and there was no statistical difference between two groups(t= 1.500,P>0.05).Conclusions (1)With the increase of tube tension,the display capacity of display device gradually strengthened.When the tube tension exceeded 90 kV, the increase of image quality on monitor was not evident.(2) With proper radiation dose, the value of tube tension in digital X-ray chest photograph was about 90 kV.