Objective To observe the effect of aerosol inhalation of ambroxol combined with budesonide on neonatal pneumonia .Methods 66 infants with pneumonia treated were enrolled in this study.The patients were divided into observation group ( n =33 ) and control group ( n =33 ) according to the random number method.The treatment group were treated with ambroxol combined with budesonide and the control group were treated with budesonide.The two groups of infant lung function, clinical outcomes and blood flow were compared.Results The total effective rate (93.94%) in the observation group was significantly higher than that in the control group (45.45%), there was significant difference between the two groups (P<0.05).After treatment, FEV1 and PEF in the two groups were significantly higher than those before treatment, the observation group [(95.21 ± 7.69)%, (93.21 ±7.89)%] were significantly higher than those of the control group[(87.03 ±7.23)%, (84.55 ±7.45)%], the differences between the two groups were statistically significant (P<0.05).The OI of the two groups was significantly higher than that of the control group and the OI of the observation group was significantly higher than that of the control group.PaCO2 of the two groups decreased significantly and the PaCO2 of the observation group was significantly lower than that of the control group, the difference was statistically significant ( P<0.05 ).Conclusion The combination of ambroxol and budesonide is effective in the treatment of neonatal pneumonia.It can effectively improve the infant pulmonary function, shorten the treatment time and improve the hemodynamics of infants .

BACKGROUND: In order to enhance the rate and degree of osseointegration of cementless implants, hydroxyapatile(HA) was applied on the different prosthesissurfaces. Themajoritiesofthesestudieshaveutilized non-weight-bearing in vivo dog models and have focused on the fixation of the femoral component. What is the osseointegration of macrotextured acetabular components in a weight-bearing model(dog) like?OBJECTIVE:To evaluate the various macrotextured surface coatings applied to acetabular components of the same design in aweight-bearing canine total hip replacement model.DESIGN: Randomized conparative study using theexperimentalanimals as subjectsSETTING: Department of Ortopaedics, Beijing Tongren Hospital;PathologyDepartment, Bone and Joint Laboratory,St. Michael' s Hospital, U of T,Toronto,CanadaMATERIALS: The experiment was completed in the Pathology Department,Bone and Joint laboratory, St. Michael' s Hospital, U of T, Toronto, Canada from October 2001 to December 2001. Acetabular components with the selected surface characteristics were implanted into forty dogs with the body mass of 29 - 44 kg of either gender and divided into seven groups, including group 1 (AD/HA), Group 2 (CoCr beads/PA), Group 3 (CPTi bead surface),Group 4(CPTi 30 PPI foam),Group 5(CPTi 30 PPI/PA foam),Group 6(CPTi 40 PPI foam), Group 7(CPTi 40 PPI/PA foam).METHODS: Exercise was restricted to leash walks for the first fourteen days post-operatively. After this, dogs were allowed unrestricted activity. The dogs were euthanized at either three or sixmonths after surgery as the project' s protocol. The whole acetabulum,divided into weight-bearing and non weightbearing areas, containing the component was harvested and submitted for histological and histomorphological assessment. MAIN OUTCOME MEASURES:The bone ongrowth and ingrowth with weight bearing and without weight bearing areas in each group.RESULTS:There was significantly greater bone apposition and bone ingrowth for implants withHAcoatingsascompared to uncoated implants. There was also significantly greater bone ingrowth in weight-bearing areas as compared tonon weight-bearing areas at both time periods. The bone growth into AD/HA was 17％ at 3 months and descended to 14％ at 6 months. The bone growth into CPTi beads surface was 21％ at 3 months,30％ at 6months in weight-bearing areas. All four foam-coating surfaces there were 15％ to 21％ ongrowth at 3 months,15％ to 32％ at 6 months and 21％to 32％ingrowth at 3 months,29％to 45％at 6 months in weight-bearing areas( P ＜ 0.05).CONCLUSION: Hydroxyapatite coating produces significantly greater boneingrowth andappositionin all thesurfacetreatmentstestedinthis study. Weightbearingproducesagreater boneingrowththannon weight-bearing areas and obviously increases with the time prolonging, especially the foam coating

Objective To discuss curative effects of naloxone in treatment of newborns with hypoxic-ischemic encephalopathy(HIE).Methods 100 newborns with HIE were divided into two groups at random,the naloxone therapeutic group and the is routine therapeutic group.The therapy effects were compared.Results The total effective rate of naloxone therapeutic group was 92%,and the total effective rate of matched control group was 72%.There were outstanding differences between two groups.Conclusion Naloxone shall be used to treat newborn with HIE based on the correct judgment of symptom and suitable treatment as early as possible.It's an effective way to black the development of the state of HIE,and to relieve the brain injury.

Objective To study the clinical investigation of andrographis paniculate in treatment of infantile respiratory infection.Methods 260 cases who were confirmed diagnosed of infantile respiratory infection were divided into the therapy group with androgrophis paniculate and the control group with ribavirin at random.Results The therapy group was better than the control group,and there were outstanding differences between two study groups.Conclusion Andrographis paniculate can shorten the course of the disease,and improve the patients' symptoms and it is more safety than the ribavirin.

ABSTRACT:Objective To investigate the regulation of adenovirus type 36 infection on precursor protein particles progranulin expression in Uygur obese patients.Methods Based on the diagnostic criteria of obesity,the samples were divided into obese group and non-obese group.Serum neutralization test was used to detect the antibody of Ad36.The progranulin mRNA expressions in abdominal omental and subcutaneous adipose tissues were detected by real-time quantitative PCR.ELISA method was used to determine serum progranulin protein levels. CD68 protein expression of macrophage was detected by immunohistochemistry. Results ① In the Uygur population of our study,compared with that in the non-obese group (38/1 1 7,32.5%),the number of obese patients (54/1 1 5,47.0%)infected with Ad36 was significantly higher than that in non-obese group (P <0.05 ).② Serum progranulin was significantly increased in the Ad36-infected obese group (408.45±1 56.92)than in non-obese group (326.1 1±1 58.60)(P <0.05).The mRNA expression of progranulin did not differ between the two groups.③ The macrophage infiltration was significantly higher in the Ad36-infected obese group (14 730.1 6 ± 2 227.39 )than in non-obese group (10 786.50 ± 2 772.80 )(P < 0.05 ).Conclusion Ad36 infection may be associated with the occurrence of obesity in Uygur population,and adenovirus type 36 infection may regulate the expression of serum progranulin at the protein level.

[Objective] To construct a database for the genetic polymorphism of 18 STR loci (D8S1179, D21S11, D7S820, CSFIPO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA, PentaE, PentaD, SE33) in Hart population from Zhejiang province. To investigate the application of 18 STR loci in the field of paternity testing and prenatal diagnosis. [Methods] Fluorescent dye labeling multiplex STR-PCR, capillary electrophoresis and DNA sequencer GeneScan were adopted in genotyping 598 unrelated samples collected from Han population in Zhejiang province. 18-STR database was established and analyzed. Population comparison was conducted between Han population in Zhejiang province and 8 other population. 15-STR and 18-STR identification system were compared in 497 paternity testing cases. [Results] We observed the distribution of 18 STR loci in Han population meet Hardy-Weinberge equilibrium and was different from other 8 population (X~2 test, P>0.05). Statistical results showed that the heterozygosis (He) ranged from 0.630 to 0.942. The combined power of discrimination was>0.9999999999. Compared with 15-STR identification system, higher paternity index scores and higher exclusion rate were obtained with 18-STR identification system in dual-case paternity test and mutation identification. One trisomy 21 fetus was found in a prenatal paternity test case which had two characteristic genotypes in 2 STR loci of D21S11 and Penta D. [Conclusions] The 18 loci were relatively highly genetic polymorphic in Zhejiang Han population and could be used for paternity testing. Some STR loci could be used in prenatal diagnosis for aneuploidy.

Objective To identify etiological factors for non-ulcer dyspepsia (NUD) in elderly patients presenting to the emergency department.Methods A total of 97 elderly patients with NUD presenting to the emergency department from January 2011 to December 2013 were enrolled in the NUD group and 116 elderly people undergoing annual physical examinations served as the control group.Clinical data,including Helicobacter pylori (HP) infection,unhealthy living habits,anxiety,depression and regular intake,were collected and compared between the two groups.Results Compared with the control group,the NUP group showed a higher HP infection rate [70.1％ (68/97)vs.54.3％ (63/116),x2 =5.565,P＜0.05],a higher incidence of unhealthy living habits (unhealthy dietary habits,smoking,drinking,and lack of regular exercise) (P＜0.05),a higher incidence of suspect anxiety/anxiety and a higher incidence of regular intake [30.9％ (30/97) vs 10.3％ (12/116),x2=14.138,P＜0.05].SerumpepsinogenⅠ(PGⅠ) [(178.7±13.6) μg/L vs.(125.2±10.5)μg/L,t=1.971,P＜0.01] and serum pepsinogen Ⅱ(PG Ⅱ) [(28.8±5.3) μg/L vs.(14.7±3.8)μg/L,t=1.960,P＜0.01] levels were also higher in the elderly NUD patients than in the control group.Conclusions HP infection,unhealthy living habits,anxiety and/or depression,and regular intake are closely associated with NUD in the elderly.Serum PGⅠ and PGⅡ could be used as markers in screening for malignant lesions in elderly patients with dyspepsia.

As a vital component in an overall laboratory animal care and use program , development of a disaster plan plays a critical role for every research institution .Currently, most of domestic institutions would draw up an“emergency operation plan , EOP”, but ignoring a practicable “business continuity plan , BCP” in establishing a disaster plan.In this article, we will discusse about the definition of disaster , how to set up an EOP, and how to establish a thorough BCP , in order to show an integrated and professional disaster plan in laboratory animal care and use program .

Objective To establish a method for detecting Aspergillus spp.by Loop-mediated isothermal amplification.Methods Aspergillus spp.specific primers were designed from the relative conservation region of the published sequence of 28S rRNA genes of A.fumigatus (GenBank accession number AY660917),A.terreus (GenBank accession number AF454183),A.flavus (GenBank accession number AF454158),and A.niger (GenBank accession number AF454169).Genomic DNA were extracted from Aspergillus standard strains,clinical control strains and clinical samples,and amplified by LAMP.The amplified results could be read with the naked eye by the coloring effect of fluorescent nucleotide dye without the DNA electrophoresis.Approximately 103 each Aspergillus conidia suspension was added to the serum from healthy volunteers,and detected these simulative clinical samples bv LAMP assay.Results The LAMP and PCR assays obtained positive results for all four Aspergillus species and 8 simulative clinical samples (double samples for each Aspergillus species) including 103 conidia,but negative in the remaining 15 non-Aspergillus species,human total blood genomic DNA,30 clinical serum samples infected with non-Aspergillus and 10 healthy volunteers.The LAMP assay had a minimum detection of 0.05-0.5pg,by means of detect different levels of 500,50,5,0.5,0.05 pg template in each reaction tube.Conclusion The results confirm that LAMP is a simple,rapid,sensitive and specific method,and can be used for detection of Aspergillus strains in clinical and environmental specimens.

Objective To study the clinical efficacy of percutaneous transluminal angioplasty (PTA) and vacuum sealing drainage technique on patients with diabetic foot and arteriosclerosis obliteration of the lower extremity.Methods Sixty-four patients were divided into two groups.Group A (32 patients) were treated by traditional methods,while 32 patients in Group B treated by vacuum sealing drainage technique in addition to percutaneous transluminal angioplasty.Results One week after surgery,ABI in group A was 0.53 ±0.17 vs 0.85 ±0.27 in group B,P =0.00.Ulcer healing rate increased from 31.25％ in group A to 81.25％ in group B,P ＜0.01.The amputation rate decreased from 56.25％ in group A to 18.75％ in group B,P =0.00.Claudication distance extended and ulcer recurrence rate in group A was 40％,while in group B it was 0,P =0.00.Conclusion The combination of PTA and vacuum sealing drainage significantly improves the prognosis of patients with diabetic foot and arteriosclerosis obliteration of the lower extremity.