Epidermal growth factor receptor ( EGFR) express highly or mutant in esophageal cancer,which causing tumor resistance to radiotherapy and leading to poor radiotherapeutic effects.Treatment with antiEGFR can get radiosensitization.There are three main types of molecular targeted research for EGFR:synthetic monoclonal antibody,small molecule tyrosine kinase inhibitors,and other.strategies modulating EGFR biosynthesis based on RNA interference.

Objective To study the optimum conditions of extraction and ? - cyclodextrin inclusion for the volatile oil from Radix Angelicae Sinensis, Rhizoma Chuanxiong and Cortex Cinnamomi in Shaofu Zhuyu Tablets. Methods The extraction conditions were evaluated by the ultimate oil extract ratio with single- factor analysis, and the inclusion technology were optimized by the inclusion output ratio and oil- bearing ratio in the inclusion with orthogonal test. Results The optimum conditions for extraction were as follows: smashing the medicinal material into coarse powder (through 10- mesh screen), adding 8 times of the water (mwater ∶ mpander=8 ∶ 1), extracting oil for 4 hours. The best conditions for inclusion were as: the ratio of Oil and ? - cyclodextrin being 1 mL ∶ 8g, and stirring for 2 hours at 50 ℃ . Conclusion The extraction and inclusion conditions in this study are optimal with high oil extract rate, reasonable inclusion process, and minimum cost.

Objective: To observe the effects of transforming growth factor-β1 (TGF-β1) and atorvastatin on expressions of collagen type I P-Smad2, Smad4 and Smad7 in human atrial ifbroblasts, and to explore the ifbrosis and anti-ifbrosis mechanisms in human atrium. Methods: Human right atrial appendage tissue was obtained from the cardiac surgery in our hospital and the atrial ifbroblasts were isolated and cultured by generations. The effects of TGF-β1 and atorvastatin on atrial ifbroblast proliferation was detected by MTT method and the effect of TGF-β1 at (0, 0.1, 1.0, 5.0, 10.0, 20.0, 50.0) ng/ml and atorvastatin at (0, 0.1, 1.0, 10.0, 100.0) μmol/L on mRNA and protein expressions of collagen type I P-Smad2, Smad4 and Smad7 in atrial ifbroblasts were examined by RT-PCR and Western-blotting analysis respectively. Results: MTT detection presented that compared to TGF-β1 at 0 ng/ml, with the intervention of TGF-β1 at (1 and 10) ng/ml, the mRNA and protein expressions of collagen type I P-Smad2, Smad4 increased,P<0.05 and the expressions of Smad7 decreased,P<0.05. Compared to TGF-β1 at 10.0 ng/ml, with the intervention of TGF-β1 + atorvastatin at 10.0 μmol/L or with atorvastatin at 10.0 μmol/L alone, the mRNA and protein expressions of collagen type I P-Smad2, Smad4 decreased,P<0.05and expressions of Smad7 increased,P<0.05. Conclusion: TGF-β1 promotes human atrial ifbroblast proliferation and collagen type I expression, while atorvastatin inhibits such proliferation and expression, the effect might be done by affecting TGF-β1/Smads pathway in human atrial ifbroblasts.

Objective To establish a RP-HPLC method for the determination of gardenoside in Xinxue Tablets and to provide evidence for ameliorating its quality criterion.Methods HPLC conditions were as follows: C18(4.6?250 mm,5 ?m)column with temperature at 25 ℃,acetonitrile-water(15∶85) as mobile phase with flow rate of 1 mL/min,and the detection wavelength at 238 nm.Results Gardenoside was well separated from other components and had good linearity in the range of 0.128～1.024 ?g(r=0.999 2).The average recovery was 98.67 %and RSD=1.57 %.Conclusion The method is accurate,reproducible,and can be used for the quality control of Xinxue Tablets.

Objective To investigate the effect of repeated psychological stress on central nerve dopamine system, and the effect of tyrosine intervention. Methods Rats were subjected to psychological stress by Communication Box model continuously for 14 days (30min/d), and fed with tyrosine in the dose of 250mg/kg, 500mg/kg or 1000mg/kg respectively. Tyrosine hydroxylase (TH) and fos protein of the brain tissue in the ventral tegumental area (VTA), nucleus accumbens (Nac) and mesoprefrontal cortex (mPFC) area were detected by immunohistochemical staining. Results In the ventral tegumental area (VTA), 5.1mm posterior to anterior fontanel, TH positive neurons were obviously less in number in psychological stress group than that in control group (P

Objective To investigate the effect of psychological stress on the function of dopamine receptor in the central nervous system in rats and the intervention effect of tyrosine. Methods Rats were continuously subjected to psychological stress by communication box model 30min a day for 14 days. They were fed with tyrosine 250mg/kg, 500mg/kg, or 1000mg/kg, respectively. The maximal bonding capacity (Bmax) and the balance dissociation constant (Kd) of D1, D2 receptor of DA at the site of ventral tegmental area (VTA), nucleus accumbens (Nac), mesoprefrontal cortex (mPFC) were determined by radioactive ligand bonding analysis. Results Bmax of the D1 receptors declined significantly in mPFC and Nac area of psychological stress treated group compared with that of control group (P

Objective To explore the incidence risk of postoperative delirium in different underlying diseases and the effective measures of postoperative recovery.Methods 978 cases of emergency surgery,included acute abdomen 324 cases,358 cases of cardiovascular disease,296 cases of cranial trauma.To analyze preoperative underlying diseases,we observed the incidence rate of postoperative delirium,and the efficacy of different care on delirium recovery was compared.Results There was a higher incidence rate of postoperative delirium in patients with coronary heart disease,high blood pressure and emphysema (P ＜ 0.05),especially in patients with multiple disease (P ＜0.01).And effective psychological intervention could increase the recovery rate of delirium.Conclusion The preoperative underlying diseases has a significant impact on the occurrence of postoperative delirium,and the psychological intervention has good effect on the recovery of delirium.

Objective To determine the effect of ABCC4 gene silencing on cell proliferation and cell cycle in human pancreatic cancer cell lines PANC1 and BxPC-3.Methods PANC1 and BxPC-3 pancreatic cancer cells were transfected with a lentivirus expressing an ABCCA short hairpin RNA (shRNA).ABCC4 mRNA and protein expression of transfected cells was determined by RT-PCR and Western blot,colony formation ability was measured by colony assay,and cell cycle change was investigated by the flow cytometric analysis.Results Lentivirus expressing an ABCC4 short hairpin RNA (shRNA) was successfully established.After transfection with shRNA lentivirus,ABCC4 mRNA and protein expression were significantly inhibited (0.28 ±0.01 vs 1.00 ±0.03,0.22 ±0.02 vs 1.00 ±0.03,P ＜0.05).Colony formation ability was significantly decreased (4 vs 65,P ＜0.05),and cell cycle was significantly blocked at G1 phase [(54.98 ±1.78) ％ vs (42.93 ± 0.88) ％,(68.55 ± 0.75) ％ vs (54.76 ± 0.29) ％].Conclusions ABCC4 gene silencing can significantly inhibit cell proliferation of human pancreatic cancer cell lines PANC1 and BxPC-3,and block the cells at G1 phase.

20/min,PaCO2 12?109 or 0.10.26 patients with sepsis infected by Gram negative bacilli were categorized as G-group,15 infected by Gram positive coccus as G+ group and 15 healthy persons as control group.All the cases were proved to have infection by body fluid examination under microscope or bacterial culture.The level of flagellin was determined by ELISA.The peripheral blood mononuclear cells(PBMC)were isolated by the density gradient centrifugation,and total RNA of PBMCs was extracted by RNA BIOzol extract.The level of TLR5 mRNA in the peripheral blood was detected by semi-quantitative RT-PCR.The statistic significance among groups was examined by Students' t test.Results Plasma flagellin levels were below the detection limit of the ELISA kit in both G+ and control groups,while the mean level of plasma flagellin amounted to 6.636?5.147ng/ml in G-group.TLR5 mRNA expression in G-group was significantly higher than that in G+ and control groups,and no significant difference was found between G+ group and control group.Conclusion Flagellin may be involved in the development of ALI through interacts with TLR5.

Objective:To evaluate the performance for the Sysmex XN20 A1 automation blood cell analyzer.Methods: The precision, contaminative rate, linear range, blank, accuracy and various sample models were verified, and low values of blood platelet were compared with the methods of microscopy and dye.Results:The contaminative rate was lower than 0.4%. The linear arrange and precision were well for the analyzer. The values of RBC, HGB and PLT were within the range of 1±0.05 and the correlation coefficients were higher than 0.975. The bias of average and constant values by the accuracy verification samples test met the demands of accuracy. And the relative differences of various sample models meet the requirements of the comparability. The variable coefficient of low values of PLT was lower than 4% by the dye method.Conclusion: The Sysmex XN20 A1 automation blood cell analyzer has the characteristics of perfect precision, accuracy, low contaminative rate, broad linear arrange and good repeatability for the low level PLT. It can be applied in clinical laboratory.