Objective: To explore the function of spleen promoting blood production through observing change of blood routine and bone marrow of anemia rats treated by supernatant of rat spleen. Methods: Two groups of anemia rats were injected with spleen supernatant of normal(group1) and spleen deficiency rat(group2), and other two groups were translated glossary with spleen homogenate of normal rat and Guipi soup. After treatment for one week, the blood routine, bone marrow and spleen index were observed. A week after withdrawing treatment ,those were observed again. Results: ①Compared with model group, the blood routine of other groups were increased in varying degrees, especially in group1, had significant different (P