Objective To investigate the feasibility and effect of recanalization of inferior vena cava with percutaneous transluminal angioplasty(PTA)by Inoue-balloon. Methods Eighty-nine patients with Budd-chiari syndrome(BCS)were treated with PTA by Inoue-balloon. Results After PTA, the median(interquartile range)diameter of hepatic segment inferior vena cava increased from 0.00(0.20 ～ 0.00) cm to 1.90(2.00 ～ 1.47)cm; (P

Alzheimer Disease ; drug therapy ; enzymology ; Amyloid Precursor Protein Secretases ; antagonists & inhibitors ; Animals ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Membrane Glycoproteins ; antagonists & inhibitors ; Molecular Targeted Therapy ; Phytotherapy ; methods ; Presenilins ; antagonists & inhibitors

BACKGROUND: Malnutrition and overnutrition are the two problems during growth and development of high-school students.OBJECTIVE: To understand the situation and relevant factors of malnutrition and overnutrition of high-school students.DESIGN: Transverse investigation was designed.SETTING: Health Supervision Institute of Chongqing Health Bureau; Department of Nutrition & Food Hygiene, Chongqing University of Medical Sciences; the Eighth Middle School of ChongqingPARTICIPANTS: Totally 896 students in 15 classes were collected from one full-time middle school in Shapingba district in Chongqing by cluster sampling method from March to May 2004. The students were excluded if they suffered from hereditary metabolic disease and secondary pathological malnutrition or obesity due to trauma or other diseases. All of participants were in the known in the investigation. Of those, 826 students filled up the questionnaire in detail (the effective rate was 92.19%), in which, 414 students were male and 412 students female, aged varied from 13 to 18 years.METHODS: The students chose were in the investigation with questionnaire and the contents of it included basic physical situation, state of sickness, situation of parents, dietary behavior, nutritional knowledge, dietary habits, etc. The students filled up the questionnaire by them own and handed over on the spot. The evaluation of student nutrition state adopted the heights and body masses in recent physical examination scale, in reference to the criteria in "height and body mass of Chinese students aged from 7 to 22 years". The standard body mass was 80% of body masses of people with same height, based on which, the standard body mass was 100%, that less than the standard by 90% was taken as malnutrition and more than it by 120% was taken as obesity. Non-conditional logistic stepwise regression was used to analyze the data so as to probe into the factors of malnutrition and obesity in middle-school students.MAIN OUTCOME MEASURES: [1] Morbidity of malnutrition and obesity in middle-school students. [2] Factors of malnutrition and obesity in middle-school students.RESULTS: Totally 826 students entered result analysis. [1] Morbidity of malnutrition and obesity: it was 9.44% and 24.21% respectively, in which,the morbidity of malnutrition.for female was higher than male [4.84%,14.08% (x2=15.1036, P ＜ 0.05)] and the morbidity of obesity in male was higher than female [30.09%, 18.2% (x2=10.5737, P ＜ 0.05)]. [2] Factors of malnutrition and obesity: significant correlation was present between malnutrition and 5 factors, named sex, indulgence in or being choosy food,sleeping time, breakfast and animal internal organs (P ＜ 0.05-0.01). Significant correlation was present between obesity and 5 factors, named sex,body mass index of father, eating velocity, midnight snack and indulgence in or being choosy food (P ＜ 0.05-0.01).CONCLUSION: It is discovered in the investigation that the morbidity of malnutrition and obesity in middle-school students are very high and that is all closely related to sex and indulgence in or being choosy food. In order to correct malnutrition and obesity in middle-school students, it is necessary to provide correct nutritional knowledge and help them arranging diet rationally and establishing healthy life pattern.

Objective Based on dual channel melting curve analysis-based assay,we developed a method to rapidly detect the drug-resistant mutations in Mycobacterium tuberculosis through real-time PCR.Methods According to the common first-line drug-resistant mutations of Mycobacterium tuberculosis,we designed six dual-labeled fluorescence probes to rapidly detect the drug-resistant mutations through realtime PCR melting curve after amplifications of drug-resistant related gene region of DNA.The targets include rpoB 81 bp core region,katG315,inhA promoter,ahpC promoter and embB306.To validate the sensitivity and specificity of our method,we performed real-time PCR assays to detect drug-resistant mutations in 76 clinical MDR-TB samples,which were collected by Shanghai CDC in 2008.Results In the validation,this method successfully detected drug-resistant mutations in all 76 clinical MDR-TB samples.The △Tm of mutations were from 1.8 to 14.4 ℃.Comparing with the sequencing data,all mutations covered by the six probes were detected with 100％ sensitivity and 100％ specificity (rpoB,80/80; inhA,7/7 ; katG315,59/ 59;ahpC,8/8;embB306,27/27).This method can successfully detect drug-resistant mutations from 100 copies/μl DNA samples.Conclusions A widely applicable real-time PCR assay to detect first line drug-resistant mutations of Mycobacterium tuberculosis has benn developed.This method has proven to have the advantages of high sensitivity,specificity and low risk of contamination.It can be used in rapid diagnosis of clinical drug-resistant tuberculosis and the evaluation of laboratory drug sensitivity test.

Bicuspid ; Dental Pulp Cavity ; pathology ; Humans ; Mandible ; Root Canal Therapy

Child, Preschool ; Female ; Humans ; Infant ; Male ; Scurvy

OBJECTIVETo observe the clinical efficacy of comprehensive Western medical treatment plus Compound Yindan Decoction (CYD) in treatment of acute cholestatic hepatitis (ACH).

METHODSUsing randomized controlled study, 60 ACH patients in line with inclusive criteria were randomly assigned to the treatment group (treated by comprehensive Western medical treatment plus CYD) and the control group (treated by comprehensive Western medical treatment alone), 30 in each group. Scores for symptoms and levels of liver functions [total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), total biliary acid (TBA)] were observed before and after treatment.

RESULTSCompared with before treatment in the same group, total scores for symptoms decreased in the treatment group and the control group at the end of the 1st and the 4th week after treatment (all P < 0.05). Compared with the control group, total scores for symptoms decreased in the treatment group at the end of the 1st week (P < 0.05). Compared with before treatment, serum levels of TBIL, DBIL, ALP, GGT, and TBA all decreased in the two groups at the end of the 4th week after treat- ment (P < 0.01). Compared with the control group, serum levels of TBIL, DBIL, ALP, GGT, and TBA all decreased in the treatment group at the end of the 1st and the 2nd week after treatment (P < 0.05). Compared with the control group, the average time for TBIL and DBIL decreasing to the level less than five times the normal value was significantly shorter in the treatment group (P < 0.05).

CONCLUSIONCYD could significantly improve clinical symptoms of ACH patients, decrease serum levels of TBIL and DBIL, reduce serum levels of ALP, GGT, and TBA, obviously improve cholestasis, and promote the recovery.

Alkaline Phosphatase ; Bilirubin ; Cholestasis ; Combined Modality Therapy ; Drugs, Chinese Herbal ; therapeutic use ; Hepatitis ; drug therapy ; Humans ; gamma-Glutamyltransferase

220.6 ?mol/L in serums.Conclusions TORCH infection is common in infantile hepatitis in hospital.The infection rates caused by CMV are higher than those caused by HSV,TOX,RV and the rate caused by TOX is lower than others,and then the injury of liver caused by CMV is more severe than others.Neonates less than 7 days always have more organs injuried than the elders.

Objective To explore the correlation of rhinovirus infection and the morbidity of asthma in children.Methods The RV gene in nasopharyngeal secretion of 30 children with asthma acute exacerbation(asthma group),30 children in asthma clinical remission(remission group) and 30 healthy children (control group) were detected by reverse transcription-polymerase chain reaction(RT-PCR).The total serum IgE(T-IgE) was determined by chemiluminescence.The eosinophil(EOS) count (EOS%) of peripheral blood and lung function were also measured.Results The rhinovirus infection rate was 36.67% in asthma group and 3.33% in remission group.No rhinovirus was detected in control group.The rate of rhinovirus infections in asthma group was significantly highter than that in remission group and control group(?2=7.50,13.47 Pa0.05). FEV1% was (62.73?13.54)% in asthma rhinovirus infection and (86.42?17.78)% masthma with no rhinovirus infection.There was remarkable difference between 2 groups(F=14.553 P﹤0.05). The T-IgE was (836.32?44.801) kU/L and EOS% was 10.63?4.09 in asthma group with rhinovirus infection.The total IgE was (439.10?231.28) kU/L and EOS% was 5.04?2.64 in asthma group with no rhinovirus infection.There was significant difference between 2 groups(U=2.475,F=20.806 Pa0.05).Conclusions There is a close correlation between rhinovirus infection and acute attack of asthma(or worsening asthma) in children. Asthma exacerbations would be likely to happened easier because of rhinovirus infection in asthmatic children with high T-IgE.