The acurate diagnosis of invasive fungal infection(IFI)is the key point for the treatment and proginosis for IFI patients.Pathological diagnosis is still considered as the gold standard for the IFI diagnosis although difficult.The regular procedures for IFI diagnosis including culture and direct examintion are often low yeilding,the discrimination for contamination and infection is hard.Imaging manifestations are nonspecific but have important suggestive values.The detection for molds antigen content is useful,but its sensitivity and specifity need to be improved.The proper diagnosis for IFI relys on the combination of clinical characteristic,imaging manifestion and serial observation.

Objective To evaluate the changes of reticulated platelets (RP)in diagnosis and treatment of idiopathic thrombocytopenic purpura (ITP). Methods RPs were measured by flow cytometry and the percentage and absolute counts of RPs were calcula- ted in ITP group,non-ITP group and control group.Results Compared with control group,ITP group had a significantly high percentage and low absolute counts of RPs (P

AlM: To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γcoactivator-1α ( PGC-1α) on vascular endothelial growth factor ( VEGF) expression in human retinal vascular endothelial cells ( HRVEC) .METHODS:Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic ( 20%O2 ) or hypoxic ( 1% O2 ) environment for another 16h. The expression of VEGF mRNA and protein were detected by real - time PCR, ELlSA and immunofluorescence cytochemistry.RESULTS: VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC - 1αprotein both under normoxia and hypoxia conditions as compared with control groups (P<0. 01).CONCLUSlON: PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.

Heart failure is a syndrom of heart disease with high morbidity and mortality .There are more and more evidence indicating immune activation developing important role in the occurrence and development of heart failure . Interleukin (IL)‐35 ,known as a negative regulatory factor of one of the members of IL‐12 family ,participates pathogenetic process of heart failure .The present article made a review for influence of IL‐35 on heart failure .

Nowadays, LAN can't meet the requirements of field communication, and WLAN is playing more and more important role for its strong transmission ability and high mobility. This paper introduces the application of WLAN to filed medicine.

Objective To know the levels of polychlorinated dibenzo-p-dioxins(PCDDs),polychlorinated dibenzo-p-furan (PCDFs)and polychlorinated biphenyl(PCBs)in milk and milk powder.Methods From Feb.2007 to Jun.2007,the milk and milk powder samples were collected and PCDDs,PCDFs and PCBs were extracted from the samples by Soxhlet extraction,cleaned up by FMS and quantified by HRGC-HRMS,using isotope dilution methodology.Results PCDDrFs and PCBs were detected in all samples.The mean levels of PCDD/Fs and dl-PCBs TEQ in the milk powder were 0.43 pg/g lipid(median:0.34 pg/g lipid),and in the packed milk were 3.83pg/g)lipid(median:2.04 pg/g lipid).The mean levels of PCDD/Fs and dl-PCBs TEQ in all samples were 2.13 pg/g lipid(median:0.815 pg/g lipid).The levels of PCDD/Fs and dioxin-like PCBs in the detected samples were far below the limitation of EU except of two milk samples,and the levels of PCDD/Fs and dioxin-like PCBs were higher in the packed milk than those in the milk powder.Conclusion Some of the milk and milk powder in the investigated city has been polluted by polychlorinated dibenzo-p-dioxins(PCDDs),polychlorinated dibenzo-p-furan(PCDFs)and polychlorinated biphenyl(PCBs).

Objective To explore the clinical value of B ultrasound-guided psychro-circulation percutaneous microwave coagulation therapy(PMCT) in the treatment of liver cancer.Methods One hundred and sixteen liver cancer patients with 154 lesions were selected to receive psychro-circulation PMCT in our department from July 2004 to December 2007.The pre-and postoperative alpha fetoprotein(AFP) levels were detected.The diameters and blood flow state of the tumors were measured and compared by use of B ultrasound and computerized tomographic scanning(CT).The postoperative 1,2 and 3 y survival rate of those patients was followed up and recorded.Results No mortality or severe complications occurred in this series.Preoperative serum AFP level of the patients was(421.6?36.5)?g/L and decreased obviously to(232.5?25.6)?g/L at 1 month after psychro-circulation PMCT(P

10.3969/j.issn.1008-9691.2013.03.010

For better adaptation to clinical work and scientific research as well as self-development,medical postgraduates are required to improve their reading ability to harvest information from medical English articles.Constructing formal schema based on linguistic schema and enriching content schema can significantly enhance medical English reading ability,according to schema theory and education practice.Therefore,the learning mode was explored based on schema theory and this theory was applied in practice to elevate reading ability by means of reading report.

Objective To establish oxidative stress model of hydrogen peroxide treatment by using human umbilical vein endothelial cells (ECS) as cell model to study the protective mechanism of anti oxidative stress and determine the signal transduction pathway of remifentanil.Methods Primary cultured human umbilical vein endothelial cells which were incubated with 0.1 M hydrogen peroxide to establish injury model to study remifentanil protection and related pathways.The experiment was divided into nine groups: control group (group C), Hydrogen peroxide group (group H1), Hydrogen peroxide+SP600125 group (group H2), Hydrogen peroxide+SB203580 group (group H3), Hydrogen peroxide+PD98059 group (group H4), hydrogen peroxide+remifentanil group (group HR1), hydrogen peroxide+remifentanil+SP600125 group (group HR2), hydrogen peroxide+remifentanil+SB203580 group (group HR3), hydrogen peroxide+remifentanil+PD98059 group (group HR4).Groups H1, H2, H3 and H4 only performed MAPK pathway blockade experiments.Groups HR1, HR2, HR3 and HR4 individually added remifentanil 10 ng/ml to protect 1 h.SOD activity, MDA level, Caspase-3 activity were detected and anti oxidative stress of remifentanil observed to confirm preliminary transduction pathway;Using RT-PCR expression levels of c-Jun before was observed before and after treated with remifentail 10 ng/ml.The aim was to determine the transduction pathway of the signaling molecules.Results Compared with group C, SOD activity were decreased significantly, MDA performance level were increased significantly in groups H1, H2, H3 and H4 (P<0.05).Compared with group H1, SOD activity was increased significantly, MDA performance level was decreased significantly in group HR1 (P<0.05).SOD activity difference and MDA performance level of groups HR2 and H2 had no statistical significance.Compared with group H3, SOD activity was increased significantly, MDA performance level was decreased siginificantly in group HR3 (P<0.05).Compared with group H4, SOD activity was increased significantly, MDA performance level was decreased significantly in group HR4 (P<0.05).Caspase-3 activity of groups H1, H2, H3 and H4 were higher significantly than that of group C (P<0.05).The level of C-Jun mRNA in group H1 was significantly higher than that of group C;But it was higher in group HR1 than that of group C, it was significantly lower than that of group H1 (P<0.05).Conclusion By activating the JNK pathway and its downstream signaling molecule c-Jun, remifentanil 10 ng/ml has the effect of increasing SOD activity, reducing the level of MDA expression and playing a role in anti oxidative stress.