2.Structure,Function and Evolution of Baculoviral Inhibitor of Apoptosis Protein Gene
Rui ZHANG ; Qing YAO ; Jian-Xin PENG ; Hua-Zhu HONG ;
Microbiology 1992;0(01):-
Baculoviral IAP(inhibitor of apoptosis protein) gene was identified firstly in IAP gene family.The structurcal feature of baculoviral IAP genes are characterized BIR and RING domain;Despite similar to P35 in antiapoptotic function,baculovrial IAP and P35 differ in structure and mechnism of action.Phylogenetic analysis of IAP genes and lots of evidence sppport the origin of this viral gene by capture of a host gene early in the evolution of Lepidoptera.
3.Research Advances Baculovirus DNA polymerase gene
Yuan-Yuan QIAO ; Rong PENG ; Jian-Xin PENG ; Hua-Zhu HONG ;
Microbiology 1992;0(03):-
Baculovirus DNA polymerase gene belongs to an early gene of baculovirus. It is a necessary gene required for replication of virus in insect cells. It can encode DNA polymerase induced by virus. In the process of replication, DNA polymerase can bind to homologous regions and non-homologous regions, which are believed to act as the origins of virus DNA replication with other replication factors. In addition, DNA polymerase has advantages over occlusion protein and egt gene for resolving deep branching taxonomic relationships of baculovirus phylogenies.
4.Research progress and medicinal chemistry strategies of URAT1 inhibitors
Xiao-yu SHI ; Tong ZHAO ; Jian ZHANG ; Rui-peng LIANG ; Zhi-jiao ZHANG ; Xin-yong LIU ; Peng ZHAN
Acta Pharmaceutica Sinica 2022;57(10):2960-2971
The urate transporter 1 (URAT1) which controls urate reabsorption is a membrane transporter in the apical membrane of human renal proximal tubule epithelial cells. It was found that about 90% of patients suffer from hyperuricemia due to insufficient uric acid excretion. Therefore, the development of URAT1 inhibitors that can reduce the level of serum uric acid
5.Influence of Ginkgo biloba extract on proliferation of ACC-2 cell, Survivin and TIP30 gene expression in adenoid cystic carcinoma of lacrimal gland.
Po NIU ; Xin-xia ZHAO ; Fei YAN ; Yong-ming ZHOU ; Peng JIAN
China Journal of Chinese Materia Medica 2014;39(24):4860-4864
Exploring the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell in lacrimal adenoid cystic carcinoma and analyzing the influence of EGB on the gene expression of Survivin and TIP30 based on the levels of the gene and protein. ACC-2 cell in human with ACC of lacrimal gland disposed by EGB of different concentration was in vitro cultured. MTT method was used for cell proliferation detection. Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin and TIP30 gene expression together with protein expression were analyzed by RT-PCR and Western blotting. And it is indicated that EGB has inhibitory effect on the proliferation of ACC-2 cell in vitro. Furthermore, the dose-effect relationship was significant. Compared with the control group, it had statistical difference (P <0.01). The inhibitory concentration 50% (ICso) is 88 mg . L-1. By flow cytometer examination, it was indicated that EGB can gradually increase ACC-2 cell in G0-G1 stage and decrease it in G2-M and S stage. With the increase of dose, the apoptosis rate of ACC-2 cell obviously increased (P <0.05 or P <0.01). Both of the expression results of RT-PCR and Western hybrid proteins have showed that the concentration of EGB increased, it could be seen a significant decrease in Survivin gene expression (P <0.01). Meanwhile, the TIP30 gene expression got a significant increase. Therefore, EGB can effectively inhibit ACC-2 cell Survivin gene expression in human with adenoid cysistic carcinoma of larcrimal gland as well as promoting TIP30 gene expression, inducing the ACC-2 cell apoptosis and inhibiting tumor cell proliferation, which provided a certain theoretical and experimental basis for the application of Chinese herbal medicinal ingredient in the treatment of tumors.
Apoptosis
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drug effects
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Apoptosis Regulatory Proteins
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metabolism
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Carcinoma, Adenoid Cystic
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drug therapy
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Cell Cycle
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Cell Line, Tumor
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Cell Proliferation
;
drug effects
;
Drugs, Chinese Herbal
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Gene Expression
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Ginkgo biloba
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chemistry
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Humans
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Lacrimal Apparatus
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drug effects
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Plant Extracts
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chemistry
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isolation & purification
;
pharmacology
6.Pharmacophore identification of novel dual-target compounds targeting AChE and PARP-1.
Xin-Lei GUAN ; Feng-Chao JIANG ; Yue WANG ; Peng-Fei WU ; Fang WANG ; Jian-Guo CHEN
Acta Pharmaceutica Sinica 2014;49(6):819-823
Multi-target drugs attract increasing attentions for the therapy of complicated neurodegenerative diseases. In this study, a computer-assisted strategy was applied to search for multi-target compounds by the pharmacophore matching. This strategy has been successfully used to design dual-target inhibitor models against both the acetylcholinesterase (AChE) and poly (ADP-ribose) polymerase-1 (PARP-1). Based on two pharmacophore models matching and physicochemical properties filtering, one hit was identified which could inhibit AChE with IC50 value of (0.337 +/- 0.052) micromol x L(-1) and PARP-1 by 24.6% at 1 micromol x L(-1).
Acetylcholinesterase
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metabolism
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Cholinesterase Inhibitors
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pharmacology
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Computer-Aided Design
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Drug Discovery
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methods
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Poly(ADP-ribose) Polymerase Inhibitors
7.Diagnosis and treatment of cerebellum abscess in 2 cases.
De-zhi YU ; Jian-xin QIU ; Xiao-peng HU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(4):334-335
Brain Abscess
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diagnosis
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etiology
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therapy
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Cerebellar Diseases
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diagnosis
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etiology
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therapy
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Ear Diseases
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complications
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Humans
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Male
;
Young Adult
8.Clinicopathologic features of large-cell calcifying Sertoli cell tumor of the testis.
Jian-wei ZHANG ; Xin-min GOU ; Zhi LI ; Ting-sheng PENG
Chinese Journal of Pathology 2007;36(4):281-282
Adult
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Calcinosis
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metabolism
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pathology
;
surgery
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Humans
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Inhibins
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metabolism
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Male
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Orchiectomy
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S100 Proteins
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metabolism
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Sertoli Cell Tumor
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metabolism
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pathology
;
surgery
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Testicular Neoplasms
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metabolism
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pathology
;
surgery
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Testis
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metabolism
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pathology
;
surgery
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Vimentin
;
metabolism
9.Integration of burn treatment and rehabilitation for a child with extremely severe burn.
Hongming LI ; Jiaping ZHANG ; Jian CHEN ; Huapei SONG ; Qiushi LIU ; Xin FAN ; Yizhi PENG ; Jun WU
Chinese Journal of Burns 2015;31(2):130-134
This article reports the successful experience of integration of burn treatment and rehabilitation for a child suffering from 91% TBSA flame burn injury (with 60% TBSA full-thickness injury, 30% TBSA deep partial-thickness injury, and 1% TBSA superficial partial-thickness injury), severe inhalation injury, severe burn shock, stress ulcer, gastrointestinal bleeding and atelectasis of the right upper lung. The patient was given effective fluid infusion against shock, treatment for gastrointestinal bleeding, and other effective supportive treatment for functions of various organs after being admitted to our burn ward. When vital signs became stable at 30 hours post injury, bedside rehabilitation was begun. On post injury day (PID) 4, escharectomy was performed for both lower limbs, followed by microskin grafting and allogeneic skin covering. On PID 10, invasive infection of multi-drug resistant bacteria was found with accompanied high fever, and at the same time allograft began to disintegrate, with dissolution of large area of eschar, leading to a raw surface reaching 86% TBSA. Following debridement, dressing, application of compound polymyxin B ointment, temporary covering of wounds with porcine acellular dermal matrix, adjustment of antibiotics, patient's condition was finally stabilized. From PID 28 on, split-thickness skin grafting was conducted 7 times, and the raw surface of 75% TBSA involving the upper and lower limbs and trunk was successfully covered. At the same time, our rehabilitation team launched comprehensive rehabilitation measures comprising active exercise, occupational therapy, prevention of scar formation, organ function training and psychological intervention. Finally, the patient was able to walk unaided and fed herself when the wounds were almost entirely healed in 3 months after injury. Oriented forwards functional rehabilitation, strong cooperation between team members, and synchronous effective implementation of burn treatment and rehabilitation in the whole process are the keys to achieve successful integration of burn treatment and rehabilitation of this child.
Acellular Dermis
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Animals
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Anti-Bacterial Agents
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therapeutic use
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Burns
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rehabilitation
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therapy
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Cicatrix
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Debridement
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Humans
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Shock
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complications
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Skin
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Skin Transplantation
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Swine
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Trauma Severity Indices
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Treatment Outcome
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Wound Healing
10.The clinical effect of iontophoresis of hyaluronidase in treating infants with congenital muscular torticollis
Qin JIANG ; Peng LIU ; Chu-Huai WANG ; Jian-Xin DING ; Su-Ying LUO ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(04):-
Objective To observe the clinical effects of hyaluronidase iontophoresis in the treatment of in- fants with congenital muscular torticollis(CMT).Methods Fifty infants with congenital muscular torticollis were divided randomly into a treatment group and a control group.Manual stretching was performed with both groups,while hyaluronidase iontophoresis was also administered to those in the treatment group.The range of side-flexion and rota- tion of the neck was examined at the beginning of and after 1 and 2 months of treatment.At the end of the treatment, the overall outcome was assessed according to a scoring system.Results There was no significant difference be- tween the two groups at admission.Compared with the control group,the range of side-flexion and rotation of the neck,and the overall treatment outcome were all significantly better in the treatment group after treatment.Conclu- sion Hyaluronidase iontophoresis can effectively improve function in infants with CMT and alleviate their symptoms.