Objective To discuss the CT features of mediastinal teratoma.Methods 35 patients with mediastinal teratoma confirmed by clinical and pathologic data were retrospectively analyzed.Results Most mediastinal teratomas(97.1%) were localized in the middle or upper area of anterior mediastinum.Mediastinal teratoma included cystic teratoma(60.0%) and solid teratoma(40.0%).All kinds of tissues could be found in the tumors.CT features of cystic teratoma(n=21) were the cystic mass with fluid density,most of them(n=19) had the characteristics of "obviouswalled cysts".CT features of solid teratoma(n=14) were the soft-tissue mass with mixed densities.CT features of malignant teratoma(n=5) were that the layer of fatty tissue between the tumor and the surrounding structures disappeared,and that the wall of neighbour vessels were mostly or all surrounded by the tumor.Mediastinal teratoma could cause pleural offusion(n=6) and pericardial offusion(n=12).Conclusion Mediastinal teratomas have obvious CT imaging features.CT has very important value in the diagnosis and differential diagnosis of mediastinal teratoma.

Objective To explore the therapeutic effect of Yupingfeng powder in secondary immunodeficiency caused by simple nephrotic syndrome(SNS) in children.Methods Seventy-eight cases of SNS patients were divided into 2 groups randomly.One was control group which were treated with immunosuppressant only.The other group was Yupingfeng powder group which were treated with both immunosuppressant and Yupingfeng powder.The morbidity of infection,the relapse rate of SNS,and serum immunoglobulin IgA and IgG and the T-lymphocytes subtype were detected before and after treatment in two groups.Results The morbidity with infection was 55.3% in Yupingfeng powder group,which shows a remarkable decrease compared with control group(85%).The relapse rate of SNS was 39.5% in Yupingfeng powder group which was much lower than that in control group(67.5%).The level of serum IgA and IgG before treatment were(3.88?1.22) g/L and(0.83?0.14) g/L and rose up to(10.06?1.89) g/L and(1.38?0.10) g/L after treatment in Yupingfeng powder group.Whereas the control group with(6.23?1.55) g/L and(0.85?0.13) g/L was remarkably lower than those in Yupingfeng powder group.CD4+,CD8+ and the ratio of CD4+/CD8+ were(37.91?3.89)%,(30.69?3.08)% and(1.24?0.49)% respectively before treatment.They rose up to(42.38?4.89)%,(26.01?2.20)% and(1.63?0.41)% respectively in Yupingfeng powder group,while those in control group were(39.87?3.91)%,(29.76?3.55)% and(1.34?0.24)% respectively after treatment.Conclusion It is feasible to treat the Yupingfeng powder to cure the of secondary immunodeficiency caused by SNS in children.

Purpose To establish a golden hamster model of intrahepatic cholangiocarcinoma (ICC) using BOP [N-nitrosobis (2-oxo-propyl) amine] and to explore the protein expression of Wisp-1,β-catenin, TGF-β1 and Smad4 in ICC and their relationship with the tumorigenesis. Methods 57 female golden hamsters aged 8 to 9 weeks (39 in experimental group, 18 in control group), the experi-mental animals were subjected to subcutaneous injection of BOP, the control group was injected with saline. The liver was removed and paraffin sections were prepared for histopathological observation. The protein expression of Wisp-1,β-catenin, TGF-β1 and Smad4 was detected by immunohistochemistry (IHC) in these blocks. Results Most of the animals in the experimental group (29/39) developed ICC, part of the animal (8/39) developed bile duct dysplasia, 1 developed focal bile duct hyperplasia, and 1 was not found bile duct hyperplasia. The positive expression rates of four protein markers in ICC, bile duct dysplasia and normal intrahepatic bile duct tissues were Wisp-1,79. 3%, 87. 5% and 5. 0%,β-catenin, 96. 6%, 100. 0% and 15. 0%, Smad4, 96. 6%, 100. 0% and 25. 0%, TGF-β1, 62. 1%, 12. 5% and 5. 0%, respectively. The positive expression rates of Wisp-1, beta-catenin and Smad4 protein in both the ICC and the tissue of bile duct dysplasia were higher than that of normal intrahepatic bile duct tissue ( P<0. 001 ) , The positive ex-pression of TGF-β1 in the ICC tissue was higher than that of normal intrahepatic bile duct tissue and bile duct dysplasia (P<0. 001). Conclusions The study showed that BOP can induce a golden hamster model of ICC and provides a reliable animal model for the study of ICC. The high expression of Wisp-1,β-catenin, TGF-β1 and Smad4 in BOP-induced intrahepatic cholangiocarcinoma is closely re-lated to occurrence, development and infiltration of ICC.

Objective To investigate the effects of pneumonia on gastroesophageal reflux(GER)of neonates.Methods The distal 24 h esophageal pH monitoring was performed in 30 neonates with pneumonia and 30 controls.The number of reflux episodes,the number of refluxover 5 min,the longest time of reflux,the total time of pH

Dengue virus (DENV) is a re-emerging disease transmitted by the Aedes mosquitoes and has become a major public health problem in southern China. Currently, no antiviral drug or effective vaccine exist to control this disease. The chimeric DENV structural protein vaccine cannot elicit balanced levels of protective immunity to each of the four viral serotypes; therefore, non-structural protein components may be required to construct an effective DENV vaccine. The Dengue virus non-structural 1 (DENV NS1) protein plays a critical role in viral pathogenesis and protective immunity. Therefore, immunity to Dengue 1-4 NS1 subtypes may be crucial for the prevention of severe disease. This review attempts to provide an overview about the structure and function of DENV NS1.
Animals ; Dengue ; immunology ; prevention & control ; virology ; Dengue Vaccines ; chemistry ; genetics ; immunology ; Dengue Virus ; chemistry ; genetics ; immunology ; Humans ; Viral Nonstructural Proteins ; chemistry ; genetics ; immunology

Objective To investigate the factors for retinopathy in premature infants. Methods Ophthalmologic mydri was conducted in premature infants and acording to the international classification of ROP, they were diagnosed as ROP. The risk factors of the incidence of ROP were statistically and respectively analyzed. Results Among total of 47 patients, 11 patients were found retinopathy of prematurity. Conclusion The body weight is lower, the gestation is shorter, incidence of ROP is the higher.

Objective To investigate the value of hepatic T2 value in evaluation of chronic HBV-related acute-on-chronic liver failure (HBV-ACLF).Methods The HBV-ACLF group,chronic hepatitis B group and control group who underwent liver MRI (M-GRASE sequence) were enrolled.The T2 map was produced from the post-processing software,and the mean T2 and R2 value of liver was calculated.The blood biochemical indexes from HBV-ACLF and chronic hepatitis B group were collected in 2 days pre-MR scaning.The differences of T2 and R2 values among 3 groups and the correlation between biochemical indexes and T2 value were analyzed.ROC curve was conducted to evaluate diagnostic efficiency of T2 value for HBV-ACLF.Results There were significant differences of T2value (x2 =19.074,P＜0.001) or R2 value (F=10.411,P＜0.001) among the 3 groups.The AUC of T2 value for diagnosing HBV-ACLF was 0.86 (P＜0.001),with the cut-off value 57.73 ms (R2=0.017).Moderate positive correlation was shown between T2 values and international normalized ratio (INR),prothrombin time (PT),haluronicacid (HA) values (rs =0.65,0.67,0.39,all P＜0.05),and moderate negative correlation was shown between T2 values and prothrombin activity (PTA),albumin (ALB),prealbumin (PA) values (rs =-0.67,-0.48,-0.37,all P＜0.05).Conclusion T2 or R2 value could reflect the liver function,and were correlated with some biochemical indexes,which illustrated a good diagnostic efficiency for diagnostic of HBV-ACLF.

To explore the diagnostic values of miRNA141 and miRNA375 for prostate cancer.The expressions of miRNA141 and miRNA375 in serum samples from 30 prostate cancer (PCA) patients,40 benign prostatic hyperplasia patients and 50 normal controls were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).The expression of miRNA141 in tissue samples from prostate cancer was 13.4±7.2 folds of that from benign prostatic hyperplasia while the expression of miRNA375 in tissue samples from prostate cancer was 28.4 ± 10.3 folds of that from benign prostatic hyperplasia.The differential expression of miRNA141 and miRNA375 in sera of prostate cancer suggested that miRNA141 and miRNA375 might be potential tumor markers in clinical practice.

Objective To analyze the hospitalization expenses of cancer patients covered with byitem payment and quota payment packages,and probe into the impacts on such expenses for the two payment packages.Methods Inpatient records of 600 cancer patients were sampled by random from the medical insurance databases of Zhengzhou and Fuzhou to learn their hospitalization expenses and impact factors.Results Under the by-item payment package,the expenses of urban workers’ medical insurance were found higher than those of urban residents' medical insurance,with a per capita expense of RMB 32747.70 ± 32035.01 and 23035.83 ± 22875.65 respectively.Under the quota payment package however,there were no significant differences between expenses of the two kinds of inpatients,with a per capita expense of RMB 66043.41±47562.09 and 66576.54±73417.29 respectively.Conclusion There are gaps of reimbursement level between the two basic insurance schemes,which may not disappear in a short time.Under the by-item payment package,the gap exists in the difference of perreimbursement amount; under the quota payment package,the gap is negligible between the two populations under different insurance schemes.It is recommended to make reasonable use of these different payment schemes to minimize the relative gaps in medical service accessibility caused by the difference in reimbursement level.

Objective To evaluate the preoperative CT scanning in cardiac carcinoma.Methods 52 cases of cardiac carcinoma proved by operation and pathology were analysed.Results By CT scanning,the size of the tumor,it′s local extension and relation to the adjacent structures and lymph node metastases could be demonstrated.Conclusion CT scanning plays important role in the operability of the cardiac carcinoma and the planning of treatment.