Objective Identification of viral replication in patients with HB without HBeAg is significant for estimating course and adopting appropriate therapy.This research aimed to clarify clinical signification of detecting Pre-S1Ag for identifing viral replication in patients with HB without HBeAg.Methods Sera samples were collected from 65 outpatients with HB confirmed by liver biopsy. HBV markers ,Pre-S1 antigen in sera were detected by ELISA;HBV-DNA by PCR;HBsAg, HBcAg and Pre-S1Ag in tissue by immunohistochemistry method.Results Intracellular HBsAg,Pre-S1Ag mainly expressing in the plasma account for 78.4% (51/65) ,69.2%(45/65) respectively, HBcAg partly in the plasma and the nucleus account for 67.6(44/65).Positive rate of expressing HBcAg,Pre-S1Ag are little less than HBsAg, but very near between Pre-S1Ag and HBcAg.There are no significant difference by ?~2 test (P=0.736). There are no significant difference between HBV-DNA (≥10~3copies/ml) and Pre-S1Ag(P=0.059),but there are significant difference HBV-DNA-positive are 18, in which 14 are Pre-S1Ag-positive.It demonstrates that there are viral replication in 18 of 30 HBeAg-negative patients and Pre-S1Ag corresponds considerably with HBV-DNA. Among 14 HBeAg-negative patients there are 4 cases with Pre-S1Ag without HBeAg because of Pre-C mutation.Conclusion Pre-S1 is a valuably serological marker for HBV replication without HBeAg.

Natamycin is a polyene macrolide antifungal antibiotic ,which is wide ly used in the food industry in order to prevent mould contamination .Biosynthe s is gene cluster of natamycin is discovered by the overall of progress in molecul ar biology of natamycin, including 16 open reading frames which includes the gen e for 26-member ring formation of natamycin (pimS0-pimS4 ) and the modifying gene s, and the function of the protein including polyketide synthases(PKSs)、PimD、P imJ、PimK were studied

To investigate the expression of inducible nitric oxide synthase (iNOS) in the hydropic cochlea of guinea pigs, the experiment consisted of two groups: normal control group and experimental group. The hydropic animal model was established by destroying the endolymphatic sac. The expression of iNOS in the hydropic cochlea was examined by immunohistochemistry. The results showed that the expression of iNOS in the hydropic cochlea was positive. The expression of iNOS in the area of stria vascularis and spiral ganglion cells was much stronger. It is suggested that nitric oxide is involved in the pathophysiology of cochlear dysfunction in hydropic cochlea.

The object of this study was to investigate the anti-ototoxic effect of iminoethyl-lysine on cochlea of guinea pig damaged by gen-tamicin. The experiment consisted of three groups: normal control group, experimental treatment control group and experimental treatment group. Guinea pigs of the latter two groups were subjected to hypodermic injection of gentamicin, at the same time guinea pigs of the experimental treatment group were treated with intraperitoneal injection of iminoethyl-lysine. while guinea pigs of the experimental control group were treated with the same volume of saline. Guinea pigs of the normal control group were treated with hypodermic and intraperitoneal injection of the same volume of saline as that of the other two groups. The expression of iNOS was examined in the cochleas of all groups by immunohisto-chemistry. ABR hearing threshold shift were examined, and the damage of hair cells of cochleas of all groups was investigated by scanning electronic microscopy. The results showed that iNOS expression was negative in cochleas of the normal control group, and positive in that of other two groups. The expression of iNOS in the experimental control group was much more stronger than that of the experimental treatment group. The ABR threshold shift of the experimental treatment group was much more lower than that of the experimental control group. The damage of cochlea of the experimental control group was much more severe than that of the experimental treatment group. These results suggest that the expression of iNOS is positive in gentamicin-damaged cochleas. Iminoethyl-lysine has significant anti-ototoxic effect on the cochlea damaged by gentamicin, suggesting nitric oxide plays a very important part in the process of the cochlea injury by gentamicin.

Actins ; metabolism ; Angiomyolipoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Epithelioid Cells ; metabolism ; pathology ; Female ; Gastrointestinal Neoplasms ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Kidney Neoplasms ; metabolism ; pathology ; Lung Neoplasms ; pathology ; Lymphangioleiomyomatosis ; pathology ; Male ; Melanoma ; pathology ; Melanoma-Specific Antigens ; metabolism ; Pancreatic Neoplasms ; pathology ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; Sarcoma, Clear Cell ; pathology ; Skin Neoplasms ; pathology ; Uterine Neoplasms ; pathology

Objective To assess the chest X-ray radiographic findings in children with influenza A (H1N1) virus infection. Methods The chest X-ray radiographies in 67 children with influenza A(H1N1) virus infection were reviewed in this study. The chest radiographies were obtained 3-8 days after the onset of symptoms and for the follow-up. Results The abnormalities were bilateral in 53 patients and unilateral in 7 patients. The predominant radiographic findings were bilateral patchy consolidation (n=42) with rapid confluence in 10 patients, lobular consolidation (n=7) with interstitial hyperplasia in 1 patient 3 month later, diffuse consolidation (n=11) with interstitial hyperplasia in all patients after 3 month. ConclusionThe predominant chest X-ray radiographic findings are bilateral patchy consolidation and diffuse consolidation with interstitial hyperplasia afterward.

Objective To investigate the clinical relevance of hepatic artery variation during the procedure of duodenopancreatectomy. Methods Data of 100 patients who underwent duodenopancreatectomy were retrospectively reviewed, and the anatomy of hepatic artery variation was evaluated, then the types of variation and specific intra-operative managements were recorded. Results Through pre-operative celiac artery and superior mesenteric artery DSA and duodenohepatic ligament skeletonization during operation, 16 cases were found to have hepatic artery variation, and 14 (14%) patients had alternative hepatic artery, among them there were 10 cases of alternative right hepatic artery (10%) , 8 cases originated from superior mesenteric artery, 2 cases originated from gastroduodenal artery. 4(4%) patients had alternative left hepatic artery, 3 of them originated from left gastric artery, 1 originated from right hepatic artery. The diameter of variant artery was 0.3 -0.6 cm with a mean of 0. 47 cm. All the variant arteries were reserved in operation. 1 patient had a variant hepatic artery located in the posterior of hepatoduodenal ligament parallel with portal vein, and the diameter of this variant artery was 0. 4 cm, the variant artery was reserved. 1 patient had a variant hepatic artery towards right hepatic lobe which originated from the direction of pancreatic head, and the diameter of this variant artery was 0.2 cm, the artery was dissected 1 h after artery occlusion. Conclusions Whether variant blood vessel need to be reserved shall be judged according to blood vessel diameter, the changes of liver in the course of variant artery occlusion and suggestions from blood vessel surgeon.

Objective To investigate the feasibility and safety of spleen-preserving distal pancreatetomy outside abdominal cavity. Methods We used the method of spleen-preserving distal pancreatetomy outside abdominal cavity for 6 patients of benign diseases of distal pancreas who were admitted in Gulou Hospital from December 2005 to December 2008. Results All patients underwent the operation successfully. The mean operation time was 180 minutes, the blood loss was 100～300 ml with a mean of 200ml. No patients needed blood transfusion. The mean post-operative hospital stay time was (14±5 ) days. One patient developed pancreatic fistula and was cured with non-operative management, there was no other complications. All patients were followed up from 3 to 24 months, and the results were excellent. Conclusions Spleen-preserving distal pancreatetomy outside abdominal cavity is safe and feasible, which may avoid the unnecessary splenectomy.

Objective To analyze tissue-specific expression and polymorphic regulation of the UDP - glucuronosyltransferase (UGT) 1A gene in human tissue. Methods Forty colorectal cancer tissue. 20 normal colorectal tissue and 10 normal liver tissue were studied. UGT 1A transcriptions were investigated by exon-1 specific RT-PCR. UGT 1A proteins were studied using Western blotting analysis. Meanwhile catalytic activity of microsomes was evaluated using N-hydroxy-PhiP. Results (1) UGT 1A mRNA expressions were found to be significantly down-regulated in colorectal cancer tissue as compared with the surrounding normal tissues(P

Objective To clarify whether the intravenous administration of embryonic stem cells(ESCs)could home in on injured myocardium caused by acute myocardial infarction(MI)in rats and to improve the cardiac function afterwards,and to explore the possible effect of inflammatory factor TNF-?on it.Methods ESCs were cultured in vitro and transfected with green fluorescence protein(GFP).The model of heart failure post MI in rats was established by permanent ligation of left coronary anterior descend artery.After operation the rats were administered with 10~7 ESC via tail vein every other day for 6 days.The sham and MI without ESCs infusion groups served as control.Six weeks later,the hemodynamic measurement was employed to evaluate the cardiac function.Immunohistochemistry assay was used to detect the GFP expression in heart and other organs,and the cardiac specific protein Tropinin I(Tn I)expression in GFP positive spot of heart.Meanwhile,the co-culture in vitro of ESC and neonatal rat cardiomyocytes was used to observe the effect of overexpression of TNF-?in cardiomyocytes on ESC migration.Results Six weeks after MI,cardiac function was significantly improved in rats administered with ESC in comparison with those of MI group.Histological changes demonstrated that infused ESCs formed GFP-positive grafts in infracted myocardium.Alternatively,the positive immunostaining for Tn I was found in the area corresponding to GRP positive staining.In regard to other organs,only in spleen a few GFP positive cells were found.This would indicate that circulating ESC could translocate to infracted myocardium and to form cardiac tissue.Migration assay of ESC in vitro indicated that cultured cardiomyocytes with overexpression of TNF-aobviously enhanced the migration of ESCs in comparison with cardiomyocytes without transfection. Conclusion Intravenously infused ESC could home in on infarcted myocurdium and futher differentiate into cardiomyocytes which led to the improvement of cardiac function.In the setting of acute MI the homing mechanism Could be associated with locally released inflammatory factors such as TNF-?which may play as a chemotactic agent on circulating ESC.