Objective To explore the effect of sodium butyrate(NaB) on phosphorylation/ acetylation of histone H3(ph/acH3) at G?-globin gene and A?-globin gene promoter regions in K562 cells.Methods K562 cells were devided into 2 groups:K562 cells were grown in the presence or absence of 0.5 mmol?L-1NaB for 48 h [K562(NaB) group] and untreated K562 cells group(K562 group).Semi-quantitative RT-PCR was employed to measure the levels of G?-globin mRNA and A?-globin mRNA.The real time PCR-based chromatin immunoprecipitation(ChIP) was used to detect the levels of ph/acH3 at G?-globin gene and A?-globin gene promoter regions.Results Compared with the K562 group,there was a 1.4-fold(t=-149.022,P=0.000) and 1.2-fold(t=-13.363,P=0.000) increase in G?-globin mRNA and A?-globin mRNA,respectively,in K562(NaB) group.The level of ph/acH3 at G?-globin gene and A?-globin gene promoter region increased by 2.9-fold(t=-12.833,P=0.006) and 3.2-fold(t=-10.484,P=0.000),respectively,in K562(NaB) group,compared with the K562 group.The %Input value of G?-globin and A?-globin promoter fragment was 10.0-fold(P=0.000) and 9.5-fold(P=0.000) higher than that value of Necdin gene promoter fragment in the K562(NaB) group,while the %Input value of G?-globin and A?-globin promoter fragment was 3.2-fold(P=0.000) and 2.7-fold(P=0.000) higher than that value of necdin gene promoter fragment in K562 group.Conclusions NaB improves the phosphorylation and acetylation of H3 at ?-globin gene promoter regions,and this may be one of the mechanisms of expression of ?-globin genes induced by NaB.

OBJECTIVE:To study the stability and to predicate the expiration date of betamethasone cream.METHODS:The HPLC method was used to determination the content of betamethasone,the stability test of cream was done by initial mean velocity method and strong light exposure experiment,respectively.RESULTS:The linear concentration range for betametha?sone cream was0.25～8?g/ml(r=0.9991),the average recovery rate was98.56%,RSD=0.78%;The content was highly correlated to temperature,whereas on which the light exposure did little effect.CONCLUSIONS:Betamethasone cream should be stored under room temperature and in a cool place,it is predicted that the expiration date of betamethasone cream is1year.

Abstract On August 11, 2022, Tongxiang Center for Disease Control detected a case of brucellosis, and the case was not engaged in related work. Case finding and risk factor investigation were immediately conducted to trace the source of infection. It was revealed that the case sold aquatic products at a farmer's market and frequently picked up goods at a seafood warehouse adjacent to a lamb slaughtering site. There was a potential risk of infection due to indirect contact with slaughtered lambs or contaminants. Serological tests were conducted on 9 employees of the slaughtering site and 48 residents nearby, and the brucellosis cases diagnosed in hospitals in the same area were searched. A total of 11 brucellosis cases were identified, including 9 confirmed cases and 2 asymptomatic infections. There were 2 cases of slaughtering workers and 9 cases of non-occupational individuals from the surrounding area of the slaughtering site. Brucella melitensis biovar 3 were isolated from a slaughtering worker and a non-occupational individual. The slaughtered lambs primarily came from northern regions such as Inner Mongolia and Heilongjiang. It was concluded that it was a cluster caused by Brucella melitensis biovar 3 and spread through direct or indirect contact with imported infected lambs or contaminated environments from a lamb slaughtering site. It is suggested to strengthen the quarantine of imported sheep, legally shut down non-compliant lamb slaughtering sites, implement designated slaughtering and enhance occupational protection.

Animals ; Astragalus membranaceus ; Endotoxemia ; drug therapy ; metabolism ; Interferon-gamma ; analysis ; Interleukin-4 ; analysis ; Male ; Mice ; Phytotherapy ; Plant Extracts ; therapeutic use ; Th1 Cells ; drug effects ; metabolism ; Th2 Cells ; drug effects ; metabolism

OBJECTIVETo explore the situation of quality of life (QOL) of nurses working in night shifts and the major factors influencing the QOL, and to provide scientific basis of the further effective intervention for the quality of life.

METHODSThe nurses working in night shift were sampled randomly in Grade 3A-hospitals of Tianjin, and investigated using WHO Quality of Life-BREF Scale.

RESULTSThree hundred eighty five questionnaires were collected from 398 questionnaires. The average score of subjective QOL perception was 2.90 (the full score is 5), subjective health perception was 2.64 (the full score is 5). The score of physical domain of QOL was 13.21 +/- 2.18; psychological domain of QOL was 13.38 +/- 2.28; social domain of QOL was 14.71 +/- 2.32; environmental domain of QOL was 11.36 +/- 2.31, all of which were significantly lower than norm value (P<0.01). The results analyzed by ordinal logistic regression showed that the factors affecting physical domain of QOL were years of working experience, sleep time, gastrointestinal disorders, family harmony status and regular diet; the factors affecting psychological domain of QOL were family harmony status and gastrointestinal disorders; the factors affecting social domain of QOL were the nature of nursing work and family harmony status; the factors affecting environmental domain of QOL were job title, sleep time, gastrointestinal disorders and regular diet.

CONCLUSIONThe QOL of nurses working in night shifts is lower than that of general people. The corresponding measures should be taken to raise the quality of life of nurses in night shifts.

Adult ; Female ; Humans ; Male ; Middle Aged ; Nurses ; psychology ; Quality of Life ; Surveys and Questionnaires ; Work Schedule Tolerance ; Young Adult

Objective To evaluate the treatment of hypervascular hepatic metastasis with TACE. Methods One hundred and twenty nine cases of hepatic metastasis treated by TACE were selected retrospectively and then analyzed the survival rate,clinical effectiveness and lipidol deposition quantity in tumor.Results Malformation of tumor vessels and rich blood supply were found in all cases of this study.The survival rates of 6 months,1 year and 3 years were 100%,73.6% and 26.4% respectively.The clinical effective rate was 68.2%(88/129)and no-progress rate was 23.3%(30/129).The satisfactory lipidol deposition quantity was obtained in 80.9%(97/129).Conclusions TACE is a favorable method for hepatic metastasis,and discerning the hypervascular subgroup could improve the treating effectiveness and be useful to make an appropriate planning.

Objective To compare genomic expression differences between androgenic complete hydatidiform mole (AnCHM) and normal first trimester villi with similar gestation weeks,and search for potential adjuvant diagnostic molecular markers.Methods Short tandem repeat (STR) detection was used to identify AnCHM,human oligonucleotide array U133 Plus 2.0 was used to measure genomic expression differences between AnCHM and normal villi,and quantitative fluorescent RT-PCR was used to verify array of several genes.Results Nine of 11 histologically diagnosed complete hydatidiform moles were found to be AnCHM by means of STR,and the other 2 were biparental complete hydatidifonn mole (BiCHM). Compared with villi,oligonueleotide array showed 279 genes (0.72%,279/38 500) were over expressed and 1710 genes (4.44%,1710/38 500) under expressed in AnCHM.Bioinformatics analysis found that differentially expressed genes were involved in multiple biological processes and pathways.Changes of imprinting genes,growth hormone genes and chorionie somatomammotropin hormone genes were especially remarkable.Conclusions Pathogenesis of AnCHM is a complex process involving multiple genes and pathways.Altered expression of imprint genes may play important roles in the process.

Objective To observe the therapeutic response of liver tumors by arterial embolization hyperthermia with Nano Superparamagnetic Iodized Oil(NSIO)using rabbit VX2 liver tumor model.Methods A total 24 rabbits containing experimental hepatic tumors were randomly assigned to one of four groups as follows:NSIO embolization hyperthermia group(group A),Lipidol embolization group(group B),NSIO embolization group(group C),and contronl group(group D),each groups contain 6 VX2 rabbits.Fourteen days after implantation of the experimental hepatic tumor,VX2 rabbits were treated.In group A group B and group C,the rabbits hepatic proper artery were selectively catheterized by 3 Fr microcatheters via right femoral artery under fluoroscopic guidance.10% NSIO 0.5 ml(group A and group C)or Lipidol 0.5 ml(group B)infused into proper hepatic artery.Three days after embolization,the rabbits in group A and group B were exposed to gap-type alternating magnetic field for 30 minutes,while rabbits in group C and group D have not been exposed to alternating magnetic field.The liver tumor size were measured by CT scanning before and 14 days after treatment then the animals were sacrificed,the liver,lung,heart spleen and kidney were harvested for histopathology examination,the liver tumor size were detected directly. Results All subjects experienced uneventful 14 days surivials,on the biochemical examination,there were no changes about the function of liver and renal in each group 14 days after treatment compare to pre- treatment.Fourteen days after treatment,the tumor size decreased by 8.09% in group A,but increased by 9.72% and 13.00%(P＜0.05)in group B and group C respectively,in group D,the tumor size increased by 57.50%(P＜0.01).In histopathology examination,the tumor necrosis in three treatment groups were manifest,particular in group A.Conclusion Arterial embolization hyperthermia with NSIO has obvious therapeutic response to experimental hepatic tumors,it encourages further development of this technology for the treatment of liver cancer in humans.

Objective To evaluate the mental status of malignant hematologic patients, explore the morbidity of depression in malignant hematologic patients, and investigate valid interventional treatment on them. Methods 134 malignant hematologic patients were evaluated by SDS and HAMD, and 49 patientswere selected who was diagnosed depressive disorder then randomly divided into 2 groups. One was experimental group and the other control group. The patients of experimental group were treated with antidepressant drug and mental intervention during common therapy, while the patients of control group only took common therapy. The change of immunological function after treatment was detected. Results The morbidity of depression in malignant hematologic patients was 37 %. The scores of SDS and HAMD were significandy decreased and the depressive symptoms were notablely improved in experimental group and there were significant differences after treatment and before treatment (P <0.01), but there was no significant difference in control group (P >0.1). The NPY plasma levels significantly increased after treatment in experimental group(P <0.01), but there was no significant difference in control group(P >0.05); the CD+4/CD+4 values of patients in the experimental group were significantly increased after treatments. Statistical analysis revealed significant differences in the experimental group patients between pre-treat and after-treat (P <0,05),but no obvious difference in the patients of control group (P>0.5). Conclusion Mental intervention and antidepressive treatment can improve all of the depression, immunological function and quality of life of malignant hematologic patients.