Objective:To discuss management and use for electrocardiogram (ECG) monitor during detection and repairing.Methods: To solve the problems in the process of management and use of electrocardiogram (ECG) monitor through detection and repairing of this equipment, and will discuss the management measures of ECG monitor. Furthermore, the effective quality control measures for ECG monitor will be raised.Results: Qualified rate of ECG monitor is 80.47%.It is useful to improve measure of quality control by PDCA.Conclusion: Quality control will improve the utilization rate of medical resources, and reduce investment costs of these resources.

Recently,with the recognition of blood supply and anatomy of the liver caudate lobe,the intervention therapies of hepatocellular carcinoma in caudate lobe were enriched.However,because of the complex anatomy,the treatment of hepatocellular carcinoma in caudate lobe is still controversial.The interventional methods for hepatocellular carcinoma in caudate lobe were mainly included transcatheter arterial chemoembolization (TACE),percutaneous ethanol injection (PEI),transcatheter arterial embolization (TAE),radioembolization,radiofrequency ablation (RFA) and endoscopic ultrasound-guided ethanol injection.The present statues and progresses of interventional therapy of hepatocellular carcinoma in caudate lobe were reviewed in this article.

Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip.Methods The mRNA was extracted from cancer tissue and normal lung tissue,and then was labeled by Cy5-dCTP or Cy3-dCTP.The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell carcinoma and adenocarcionma.Results Seventeen genes were up-regulated and 19 genes were down-regulated in both lung squamous cell carcinoma and adenocarcinoma,the expression of 20 genes were remarkable higher in squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenocarcinoma.Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer;cDNA chip technique might be a useful method in screening lung cancer implicated genes.

Acupuncture Therapy ; Adult ; Alcoholism ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Peripheral Nervous System Diseases ; therapy

Adolescent ; Adult ; Child ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; Musculoskeletal Manipulations ; Snoring ; therapy ; Young Adult

Functional near-infrared spectroscopy is a non-invasive optical brain function detection technology,which can do multi-point measurement of changes on cerebral cortical area corresponding to the oxygenation of the blood hemoglobin and deoxygenated hemoglobin concentration,and then reflect the state of brain cortex function.Its greatest advantage is to allow the subjects head moving in a certain range,especially suitable for children.At present this technology could be applied to clinical pediatric,such as epilepsy,autism,attention deficit/hyperactivity disorder,neonatal disease,neurosurgery and cardiovascular surgery,etc.Functional near-infrared spectroscopy technology will have more applications in the field of research on brain function.

BACKGROUND:No ideal drugs can be used in the prevention and treatment of aseptic loosening of artificial joints. Some researchs showed that erythromycin has strong inhibitory effects on periprosthetic osteolysis. Its antibacterial activity, however, limits its application in artificial joint loosening prevention. LY267108 is a new type of erythromycin derivatives, eliminates the antibacterial activities, and retains the anti-inflammatory activity.
OBJECTIVE:To evaluate inhibitory effect of LY267108 on nuclear factor kappa B during osteoclast activation.
METHODS:RANKL and macrophage colony-stimulating factor were added to RAW264.7 cellline of a mouse model induced by osteoclasts. Simultaneously, different concentrations of alendronate sodium, erythromycin and LY267108 were cocultured for 48 hours. The activity of nuclear factor kappa B and content of intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha were measured by electrophoretic mobility shift assay and western blot assay.
RESULTS AND CONCLUSION:LY267108 has a strong inhibitory effect on nuclear factor kappa B. 10 mg/L LY267108, 25 mg/L erythromycin and 10 mg/L alendronate sodium had similar inhibitory effects on nuclear factor kappa B, which was obviously stronger than 10 mg/L erythromycin. However, 25 mg/L LY267108 had strongest inhibitory effects. No significant difference in intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha levels was detected among 10 mg/L LY267108, 25 mg/L erythromycin and 10 mg/L alendronate sodium groups, but was stil apparently higher than 10 mg/L erythromycin group. Levels of intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha were highest in the 25 mg/L LY267108 group. Results indicated that LY267108 in the process of osteoclast activation had stronger inhibitory effects on nuclear factor kappa B compared with erythromycin, and its safety was higher than alendronate sodium. Simultaneously, LY267108 did not have antimicrobial activity, and became a potential ideal drug for prevention and treatment of aseptic loosening of artificial joints. However, the inhibitory effects of LY267108 on the degradation of inhibitory subunit of nuclear factor kappa B alpha would be a mechanism of inhibiting the activation of nuclear factor kappa B.

Objective To investigate the etiology of the nosocomial infection and immune function changes anus and anal after operation.Methods 130 patients who needed anus and anal canal surgery were recruited in the study.The clinical data were recor-ded and postoperative nosocomial infection data were observed,including infection type,distribution,pathogenic analysis and im-mune function.Results A total of 67 cases of nosocomial infection occured,the infection rate was 51.5% (67/130),the main types of infection were perineal wound infection,abdominal infections and pelvic infections,urinary tract and vaginal infections were rare. 93 isolates were collected,of which gram-negative bacilli accounted for 67.7% (63/93 ),including bacteroides fragilis (21.5%), Escherichia coli (18.3%),Pseudomonas aeruginosa (10.8%);Gram-positive bacteria accounted for 23.7% (30/93 ),including Staphylococcus aureus(17.2%),Staphylococcus epidermidis(6.5%);5 fungi were isolated accounting for 5.4%,other strains ac-counted for 3.2%.All the immune parameters detected in patients with postoperative nosocomial infection were statistically differ-ent from those before infection(P <0.05)except for IgM(P >0.05).Conclusion The incidence of nosocomial infection was rela-tively high in patients who had anus and anal canal surgery,which could serious affect the patients'outcomes,preventive and control measures should be taken in clinical practice.

Objective To study the growth inhibision effect of disodium cantharidinate and Vitamin B6 injection on hepatocellular carcinoma cell line HepG2.Methods Different concentrations of disodium cantharidinate and Vitamin B6 injection were applied on the hepatocellular carcinoma cell line HepG2.The inhibition rate of HepG2 growth was measured by MTT assay.The apoptotic rate was detected with flow cytometry,and the morphology of apoptosis was observed by fluorescence microscope.Results The growth of HepG2 cells was significantly inhibited as well as the apoptosis of HepG2 cells was significantly encouraged by disodium cantharidinate and Vitamin B6 injection(P

AIM: To clarify the role and mechanism of hippocampus in the formation of P3-like. METHODS: (1) The hippocampal and parietal P3-like were simultaneously recorded by chronically implanted electrodes in rats. (2) In forebrain ischemia/reperfusion model, the effects of the selective damage of hippocampal CA1 subfield on the hippocampal and parietal P3-like were examined. (3) The changes of hippocampal and parietal P3-like after the inhibition of acetylcholine activity in the septo-hippocampal pathway by intraperitoneal injection of atropine (30 mg/kg) were also detected. (4) The changes of conditional active avoidance response was tested by shuttle-box following forebrain ischemia/reperfusion. RESULTS: (1) The peak latencies of hippocampal and parietal P3-like were prolonged after forebrain ischemia/reperfusion. (2) The polarity of hippocampal P3-like were reversed after the inhibition of acetylcholine activity in the septo-hippocampal pathway by atropine. (3) The prolongation of P3-like peak latency was closely related to the lowering of the score of conditional active avoidance response after forebrain ischemia/reperfusion. CONCLUSION: (1) The formation of P3-like depends on the integrity of hippocampus formation. (2) Hippocampus is not the only generator of P3-like, P3-like could be produced in multiple way. (3) The activity of acetylcholine in the septo-hippocampal pathway has notable effects on the formation of P3-like. (4) P3-like could be used as the sensitive marker of cognitive function.