The incidence of severe acute pancreatitis (SAP) is increasing year by year,and the mortality is high,which poses a great threat to the life of patients.In recent years,the immune nutrition therapy has become the focus in the treatment of SAP,among which,fish oil lipid emulsion as a novel type of fat emulsion,has the multi-effects of anti-inflammatory,anti-oxidative,immunomodulatory,promoting signal transduction and protecting organ function apart from providing nutrition and regulating metabolism.It can prevent the deterioration of the whole body from systemic inflammatory response syndrome (SIRS) to multiple organ dysfunction syndrome (MODS and ultimately improve the prognosis of patients.

Objective To evaluate the safety and efficacy of transcatheter closure of perimembranous ventricular septal defects(VSD) using domestic symmetric double-disk occluders.Methods From March 2003 to July 2007,689 VSD patients were treated with transcatheter intervention using domestic symmetric double-disk occluders and received clinical follow up at 1 month,3 months,6months after the procedure and annually thereafter.Complications including residual shunt,heart rhythm changing,shape of the occluder and the presence of tricuspid/aortic regurgitation were observed thoroughly.Results The overall technical success rate was 98.8%.One patient died of cerebral bleeding 7 days after intervention.One patient suffered complete A-V block and required permanent pacemaker implantation and another patient conversed to open surgery because of moderate tricuspid regurgitation.Asymmetric Amplatzer occluders were used in 6 patients but the intervention failed in 2 patients whom required an open conversion.Comorbidities such as atrial septum defect,patent foramen ovale were treated concomitantly.No other complications were observed during the follow-up period.Conclusion Complication associated with transcatheter closure of perimembranous VSD using domestic symmetric double-disk occluders was relatively low.The procedure was safe and applicable.Meanwhile,it is necessary to stick to the procedural indications and avoid complications such as tricuspid regurgitation etc.

To observe the hemodynamics changes of patients undergoing laparoscopic cholecystectomy under epidural or general anesthesia. Method: 25 patients were divided into two groups: general(GA, n=11)and epidural (EA, n=14) anesthesia groups. Hemodynamic parameters (HR, MAP, CVP, PCWP, PAP, CO, CI, SVR, PVR) were measured with Swan-Ganz technique after the patients being placed to rT position,CO2 being insufflated into peritoneal cavity, and at the end of operation. Result: 1. In the EA group, CVP reduced significantly after rT position placed, SV, CO, MAP and HR reduced significanty after peritoneal insufflation,but still within normal range. 2. In the GA group, CVP, SV, CO were significant ly reduced after rT position placed; After peritoneal insufflation, all parametere were significantly increased except SV had no significant change. Conclusion:Epidural anesthesia can be safely applied to the ASA Ⅰ-Ⅱ grade patients undergoing laparoscopic cbolecystectomy.

The paper elaborates on the design thinking of the outpatient intelligent medicine delivery system under the two parallel Hospital Information Systems (HIS) of the Central Hospital of Wuhan and implements the distribution of medicine delivery windows based on the multi-channel payment methods of the old HIS outpatient system and the preplanned and real-time intelligent medicine delivery based on the new HIS pharmacy system.Using the integrated platform of Ensemble and services of Web Service,it realizes the data interaction among multiple systems.The system operates steadily and the new HIS is well connected with the old.Thus,the efficiency can be enhanced and the quality can be ensured.

Objective To compare the retromolar space (RMS) between different antero-posterior (A-P) skeletal patterns, and explore the relationship between the status of third molar eruption/impaction with the different A-P skeletal patterns. Methods A total of 90 orthodontic patients (25-35 years old) were investigated from dental pantomograms (DPTs) and lateral cephalograms (LC). The subjects were divided into three groups according to their ANB angle:skeletal classⅠ, skeletal classⅡ, and skeletal classⅢ. Each group was subdivided into impacted and erupted subgroups. Values of DPT and LC were traced, and the following variables were measured including mandibular body length, third molar angulation and ret-romolar space width. Results The retromolar space width was significantly smaller in classⅡsubjects than that in classⅢsubjects, and RMS was significantly smaller in impacted group than that in erupted group, respectively (P<0.05). There was no significant difference in the mandibular angle in erupted subgroup (P<0.05). There was a smaller mandibular body length in impacted subjects (P<0.05). Conclusion There is a relationship between different morphological characteristics of sagittal skeletal pattern and the impacted status. Patients with shorter mandibular body length and decreased RMS width are more inclined to impaction.

[Objective]To probe the probability of autologous platelet-rich glue(PRG) combined with particulate of cancellous bone in treating the aseptic tibial nonunion.[Method]Sixty patients with tibial nonunion in distal 1/3 fragment were randomly divided into 2 groups.Each group had thirty patients.PRG combined with particulate of autogenous cancellous bone was transplanted in the experimental group while particulate of cancellous bone was applied in the control group.The mean time to the operation was 10.5 months(7-36 months) and the average age were 38 years old.The postoperative systemic and local reaction,and X-ray finings of tibial nonunion were observed.The average follow-up was 22 months.According to the Johner-Wruh scoring system the results were statistically analyzed.[Result]At 8 months bony union was all achieved.There was a remarkable difference between those of experimental and control groups.[Conclusion]PRG had an important role in speeding bone healing,and the combination of PRG and particulate of autogenous cancellous bone is an ideal alternative of bone transplantation.

OBJECTIVE:To establish a cluster analysis method applied in plant classification and the identification and assessment of traditional Chinese medicine.METHODS:The raw data of the shape,the chemical composition and the UV-spectra data of plants were collected,and these data were assigned with values in accordance with the binary assignment then subjected to a cluster analysis.RESULTS:The 11 species of plants under study were accurately classified into the proper categories by the new method.CONCLUSION:By this method,the analytic data are comprehensive and the conclusion is detailed and reliable,which serves as the supplement and development for traditional cluster analysis method.

The cardiotonic activity of MD, a domestic product of AR-L115, BS, was tested and verified in isolated guinea pig atria, compared with that of isopreraline (ISO). The electrically paced left atria was used to determine their positive inotropic action, while the spontaneously beating right atria was used to determine their positive chrono-tropic action. The results demonstrate: (1) MD showed a strong positive inotropic action comparable to ISO. (2) MD also showed some positive chronotropic action, although this is much weaker than that of ISO. (3) The single dose of MD showed that the positive inotropic action developed quite slowly and strengthened gradually for a long time, while the action of ISO developed so steeply that its peak effect can reached within a few minutes. (4) Propranolol can definitely block both inotropic and chronotropic actions of ISO but scarcely those of MD. This suggests that MD is not a ?1-receptor agonist.The experimental results described above are similar to those reports abroad.

OBJECTIVETo study the effect of ethyl acetate fraction of Blumea balsamifera (BBE) residue on treating rats of adjuvant arthritis (AA) and its mechanism.

METHODThe rats were immunized with the Freund's complete adjuvant (FCA). After modeling, 28 days' treatment with BBE was performed. During the experimental process, rat mass, toe girth, arthritic index (AI), proliferation of immune organs and pathological section were measured. After treatment, blood samples were collected through fossa orbitalis vein for detection of serum SOD, MDA, GSH, NO, OH*, ALP, AST, ALT, NAG and SA content using colorimetric method and IL-1, IL-6, TNF-α content using ELISA method.

RESULTAdministration with BBE (high dose) could significantly ameliorate joint swelling and arthritis index, effectively inhibit synovial hyperplasia, down-regulate the levels of MDA, NO, OH*, ALP, AST, ALT, NAG, SA, IL-1, IL-6, TNF-α and up-regulate the SOD and GSH levels in serum.

CONCLUSIONThe results suggested BBE possesses substantial anti-arthritis and antioxidant activities.

Acetates ; Animals ; Anti-Inflammatory Agents, Non-Steroidal ; administration & dosage ; isolation & purification ; Arthritis, Experimental ; blood ; drug therapy ; metabolism ; Asteraceae ; chemistry ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; Humans ; Interleukin-1 ; blood ; Interleukin-6 ; blood ; Male ; Malondialdehyde ; blood ; Oxidative Stress ; drug effects ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; blood

Objective To evaluate the effect of glucose-insulin-potassium (GIK) on intestinal injury in endotoxemic rats.Methods Sixty male Sprague-Dawley rats, weighing 200-250 g, were equally and randonly divided into endotoxemia group (lipopolysaccharide [LPS] group) and GIK group.LPS 8 mg/kg was injected intraperitoneally once a day for 3 times in total to establish the model of endotoxemia-caused intestinal injury.Starting from 2 h after the initial injection of LPS, normal saline was continuously infused at 4 ml · kg 1 · h-t in group LPS, and GIK 4 ml · kg 1 · h 1was infused intravenously in group GIK.Before establishment of the model, and at 3 and 5 days after establishment of the model, 10 rats in each group were sacrificed, and blood samples were collected from the abdominal aorta for determination of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) and diamine oxidase (DAO) concentrations in plasma by enzyme-linked immunosorbent assay.TNF-α/IL-10 ratio was calculated.A segment of ileum of 2 cm in length, 20 cm from the ileocecal junction, was removed for microscopic examination.The degree of damage to the intestinal mucous membrane was scored according to Chiu.Results Compared with the values before establishment of the model, the plasma TNF-α/IL-10 ratio, DAO concentration, and Chiu's scores were significantly increased at 3 days after establishment of the model in the two groups, the plasma TNF-α/IL-10 ratio, DAO concentration, and Chiu's scores were increased at 5 days after establishment of the model in group LPS, and the plasma DAO concentration, and Chiu's scores were increased at 5 days after establishment of the model in group GIK (P＜0.05).Compared with the values at 3 days after establishment of the model, the TNF-α/IL-10 ratio and plasma DAO concentration were significantly increased in group LPS, and the TNF-α/IL-10 ratio and plasma DAO concentration were decreased in group GIK at 5 days after establishment of the model in group GIK (P＜0.05).Compared with group LPS, the TNF-α/IL-10 ratio, plasma DAO concentration, and Chiu's scores were significantly decreased at 3 and 5 days after establishment of the model in group G1K (P＜0.05).Conclusion GIK can reduce intestinal injury in endotoxemic rats.