Hepatocellular carcinoma (HCC)is a common malignant tumor in the world,and surgical resection and liver transplantation are two radical treatment modalities,but only 10%-20%of all patients can receive such treatments.In recent years,local therapies including radiofrequency ablation,microwave ablation,cryoablation,and the irreversible electroporation ablation which appeared recently have gradu-ally become the alternative therapies for the patients who are unable to undergo surgery.In addition to local tumor growth control and im-provement in survival outcomes,the ablation technology also helps to downgrade tumor for secondary resection.This article focuses on the re-search progress in radiofrequency ablation alone and in combination with other therapies in the treatment of HCC,compares radiofrequency ablation with other local ablative therapies,and briefly introduces the application of intelligent navigation technology in adjuvant ablation. With the development of medical imaging and progress in related fields,the ablation technology will be widely applied in clinical practice in the future.

This experiment was made on 4-6 week-old nude mice(NM). The CNE-2 cells were inoculated subcutaneously into each side of the neckback for the original generation. Implanted fresh transplanted NPC tissue to NM's neck-back with cannula for next passage. We passed up to 8 generations and the transplantability was 100%. We observed dynamicrly the changes of NM's immune status after they have bearing the NPC as following: (1) The net weight was significantly reduced. (2) Splenic index(S. I.) and tumor weight(T. W.) increased. S. I. was highly related to T. W. (r=0.8395). (3) Splenic NK activity slightly increased in 5 days, began to decrease in 10 days and significantly decreased in 15 or more days. NK activity was negative related to T. W. and S. I. markedly (r=-0.7132, r=-0.8237). (4) Splenic NK cells had excellent responsiveness to human IFN in vitro in 5 days and still had some in 10 days, but it had not any after 15 days. (5) Natural proliferative ratio of tumor bearing NM's splenic cells was higher than that of control group, but the responsiveness to ConA was much lower. (6) Ai Ke Xing suppressed the growth of transplanted NPC by means of decreasing sera MDA enhancing host's splenic NK activity and increasing mononuclear infiltration of the transplanted NPC tissue. So we concluded that in NM whose T cells were deficient splenic NK cells were important immunocompetent cells for resisting the growth of transplanted NPC.

The hospital the authom work with, the first one to undergo the second-cycle nation-wide hospital evaluation, was assessed by a third party and the hospital evaluation experts. The two cycles of hospital evaluation were entirely different in historical background, priorities and procedures. The evaluation standards for the second cycle, which were on a par with internafional ones, set the right track of development for medical institutions, emphasizing quality, security, service and performance and highlighting the delivery of quality patient care. The gains from the second-cycle hospital evaluation can be summarized as follows: more highly emphasized continuous improvement of service quality, enhanced awareness of people-oriented management, reinforced medical quality and medical security control, heightened hospital infection control, greater capability of responding to emergencies, and strengthened for-malation of hospital rules and regulations. In the end five suggestions are put forward with regard to the second-cycle hospital evaluation.

Objective To determine the potential deficiency due to lack of anaerobic culture and evaluate the effect to reduce adverse reaction associated to transfusion-translated bacterial infection.Methods The result of 9 758 units of apheresis platelet concentrates (PCs)detected with automated microbial detection system were reviewed and the medical records of the patients that received the contaminated PCs were followed.Results The confirmed positive rates by aerobic and anaerobic cultures were 0.06％ (6/9 758)and 0.16％ (16/9 758),respectively.In 10 of 16 yield cases,only the anaerobic culture was positive.The most of the bacterial detected by anaerobic culture only were Propionibacterium acnes.Their mean detection time from inoculation was 96.8±18.21 hours.Conclusion Addition of anaerobic culture would enhance the detection of bacterial contamination in PCs.However,since only slow-growing bacteria were detected,and because their clinical significance was debatable,blood service should select feasible and costeffective projects using only aerobic bottle for bacterial screening,like the majority of licensed blood centers in North America and Hong Kong,China.

Objective To assess training programs from trainees of Shanghai General Practice Teacher Training Center for further improvement.Methods Total 171 trainees attending the GP teacher training program in April,2012 were selected for survey.A self-designed questionnaires were adopted in the survey.The rationality of the curriculum and training contents in the program were analyzed.Results Among 170 trainees who answered the questionnaires,86 of them (51％) considered the training curriculum as very appropriate,76 of them (9％) considered as appropriate; 97 of them (57％) thought the training forms as very vivid,65 of them (38％) thought as vivid.The overall evaluation score for teachers was 4.64.Ninety three of the 170 trainees (55％) expressed a great willingness for training,70 (41％) expressed as willingness.Among 170 trainees 156 (92％) preferred to learn new advances in medicine,111 (65％) preferred to learn teaching skills and theories in general practice.Thirty two trainees (19％) considered that the contents of training met the trainees' needs completely,129 (76％)considered that most contents of training met the trainees' needs.Eighty two (48％) considered that they gained a lot after the training,80 (47％) considered that they gained fairly.The survey showed that 147 (86％) believed the training had broadened their minds,104 (61％) believed the training had increased their capabilities of clinical diagnosis and treatment; 53 (31％) thought the training helped raise their theoretical level.Eighty seven (51％) were very satisfied and 75 (44％) were comparatively satisfied with the training in overall evaluation.Conclusion The training program provided by Shanghai General Practice Teacher Training Center is basically satisfactory and trainees are benefited from the training considerably.

Objective To evaluate the outcomes of the standardized general practice training program in Shanghai Zhongshan Hospital,Fudan University.MethodsTotal 183 graduates of 11cohorts completed general practice standardized training in Shanghai Zhongshan Hospital,Fudan University since 2000.A questionnaire survey on the career status was conducted and 173 graduates were responded successfully with a respond rate of 95％.ResultsAmong 173 responded graduates,55 were males and 118 females with an average age of 33.8 y.One hundred and sixty nine graduates (98％) were still engaged in general practice,including 148 working in Shanghai and 21working in other cities.For professional ranks,3 of them were chief physicians,17 vice-chief physicians,81attending physicians and 72 residents (34 of them would be soon promoted to attending physician).Among the 173 graduates,39 were heads of GP teams and 49 held administrative positions.Up to February 2011,81graduates had published 184 papers as firstauthor,32 were successful in grant application and 33 research grants were awarded with a total fund of 2.487 million Yuan.Thirteen graduates obtained the chance for further study of general practice in Hong Kong,Taiwan or abroad.ConclusionThe follow-up study shows that the outcomes of general practice standardized training program in Shanghai Zhongshan Hospital are satisfactory.

Objective To evaluate the standardized general practice training program.Methods A questionnaire survey was conducted among general practitioners who completed the standardized residency training program in Zhongshan Hospital,Fudan University from January 2000 to June 2011.Results During this period 183 residents received general practice training,15 were excluded the survey due to loss of follow-up or other reasons,168 questionnaires were distributed and 165 valid questionnaires were returned with a recovery rate of 98％.The main findings:2/3 of surveyed GPs were sent by their affiliated institutions; 85％ evaluated the program as highly or very highly; 56％ thought that their expectations were completely or mostly fulfilled; 68％ thought that the program enhanced their clinical capability and theoretical levels; over half thought that the clinical rotation and strict teaching management were the advantages of the program; 84％ thought that they improved their skills,knowledge and ideas; 74％ thought that the training program was very good or fairly good; 48％ thought that the preceptors in communities were comparatively weak and some theoretical lessons were not relevant to the context of the community.The surveyed GPs required improving of practice components,shortening the total length of training and increasing the teaching contents on scientific research.Conclusion The surveyed general practitioners give credit to the GP residency training in Zhongshan Hospital and put forward some proposals for further improvement.

Objective To investigate the role of IP-10 in the pathogenesis of ankylosing spondylitis (AS). Methods The peripheral blood monocytic cells (PBMC) were collected from 39 AS patients, and the mRNA expression of IP-10, IFN-? and IL-4 were measured by semi-quantitive RT-PCR with G3PDH as the internal control. Results The mRNA expression levels of IP-10, IFN-? and IL-4 as well as the rate of IFN-?/IL-4 were elevated in the PBMC of AS patients compared with the healthy controls (P

Gene therapy of spinal cord injury (SCI) is the most promising method compared with the others, because it doesn't involve the problems of resource and higher exclusion which respectively exists in fetal nerve transplantation and peripheral nerve transplantation. There are two ways of gene therapy to be chosen: one is to transfer objective genes to the target-cells in vivo directly; the other is to transfer objective genes to one proper kind of transplantable cells firstly, then graft the highest expressing cells to the target-cells in vivo. To realize the transfer of genes to cells, two measures are used in common: physical or chemical measure such as micro infection et al and biochemical measure i. e. gene modified defective virus. Although there are some questions unresolved in this field, the clinical value of gene therapy of SCI in the future is depended.

Objective To explore the effects of low energy intravenous He-Ne laser irradiation of blood (ILIB) on cell proliferation, apoptosis and genotoxicity of lymphocytes in patients with cerebral infarction (CI). Methods The lymphocytes in peripheral blood in patients with CI were sampled before and after treatment and cultured, and then, some parameters such as the mitosis index (MI), cell cyclical kinetics index (CCKI), sister chromatid exchanges frequencies (SCEF) and apoptosis were analyzed in order to explore the effects of ILIB on lymphocytes. Results The proliferative activities of lymphocytes were significantly increased by ILIB (P0.05). After ILIB, the rate of apoptosis in patients with CI was significantly increased when compared with that before treatment and that of the control group, as revealed by flow cytometry (FCM) (P