Objective To observe the clinical effect of extracapsular cataract extraction and implantation of intraocular lens combined with vitreoretinal surgery by samall incisionto to treat cataract merge vitreoretinal disease.Methods A retrospective study was managed on 18 cases(18eyes). Eyesight and complications after operation was study. Result Follow-up period was from 3 months to 18 months( the 8 months on average). The cases of corrected visual acuity less than 0. 02 was 2 eyes, among 0.02 to 0. 1 was 4 eyes, among 0. 2 to 0.3 was 8 eyes, then more than 0.3 was 4 eyes. The complications of surgery included tunicae uveae reaction, cornea reaction, ocular hypertension, vitreous hemorrhage,retinal detachment. Conclusion Extracapsular cataract extraction and implantation of intraocular lens combined with vitreoretinal surgery by small incision was safe and effective to treat Cataract combined with vitrcoretinal disease. The main factor affected the eyesight retrieval was posterior segment pathology.

Objective To investigate the seroprevalence of HEV infection and genotype. Methods ELISA were used for detecting anti-HEV IgG of the serum samples,the nested reverse transcriptase PCR ( RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Siehuan province. The primers used for genotyping were the ORF2 region of HEV genome. Results The anti-HEV lgG was detected positive in childrens 6.10% (41/672),adults 42.26% (280/661 ),swines 88.89 % (32/36),chickens negative (0/59). 1 case of 15 serum samples of anti- HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR. Sequenee analysis of 4 isolates has 92.1% to 98.6% nucleofide sequence homology.These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology,which belonged to HEV genotype 4B. Conclusions The swine were the risk factors in the spread of hepatitis E virus.