Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip.Methods The mRNA was extracted from cancer tissue and normal lung tissue,and then was labeled by Cy5-dCTP or Cy3-dCTP.The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell carcinoma and adenocarcionma.Results Seventeen genes were up-regulated and 19 genes were down-regulated in both lung squamous cell carcinoma and adenocarcinoma,the expression of 20 genes were remarkable higher in squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenocarcinoma.Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer;cDNA chip technique might be a useful method in screening lung cancer implicated genes.

Recently,with the recognition of blood supply and anatomy of the liver caudate lobe,the intervention therapies of hepatocellular carcinoma in caudate lobe were enriched.However,because of the complex anatomy,the treatment of hepatocellular carcinoma in caudate lobe is still controversial.The interventional methods for hepatocellular carcinoma in caudate lobe were mainly included transcatheter arterial chemoembolization (TACE),percutaneous ethanol injection (PEI),transcatheter arterial embolization (TAE),radioembolization,radiofrequency ablation (RFA) and endoscopic ultrasound-guided ethanol injection.The present statues and progresses of interventional therapy of hepatocellular carcinoma in caudate lobe were reviewed in this article.

Objective:To discuss management and use for electrocardiogram (ECG) monitor during detection and repairing.Methods: To solve the problems in the process of management and use of electrocardiogram (ECG) monitor through detection and repairing of this equipment, and will discuss the management measures of ECG monitor. Furthermore, the effective quality control measures for ECG monitor will be raised.Results: Qualified rate of ECG monitor is 80.47%.It is useful to improve measure of quality control by PDCA.Conclusion: Quality control will improve the utilization rate of medical resources, and reduce investment costs of these resources.

Acupuncture Therapy ; Adult ; Alcoholism ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Peripheral Nervous System Diseases ; therapy

Objective To investigate the clinical effect of voriconazole in the treatment of invasive fungal infections in lung cancer patients with radiotherapy and chemotherapy,to provide a reference for clinical treatment. Methods The clinical data of 64 lung cancer patients with radiotherapy and chemotherapy with invasive fungal infec-tions were retrospectively analyzed.According to the treatment methods,they were divided into control group and observation group,32 cases in each group.Fluconazole treatment was used in the control group,and the observation group used voriconazole therapy.The overall response rate,time to return to normal white blood cells and other indica-tors of liver and kidney function were compared between the two groups.Results The effective rate of the observation group was 81.25%,which was significantly higher than 68.75% of the control group,the difference was statistically significant(χ2 =5.876,P=0.008).The temperature recovery,cough disappeared,leukocyte recovery time of the observation group were (3.61 ±0.61)d,(6.11 ±0.81)d,(7.85 ±0.92)d,which were significantly shorter than those of the control group,the differences were statistically significant(t=4.145,P=0.025;t=4.045,P=0.027;t=4.385,P=0.021).The treatment of the two groups had mild liver and kidney damage,and there was no signifi-cant difference between the two groups(all P>0.05 ).Conclusion Voriconazole treatment has good clinical effect for lung cancer patients after radiotherapy and chemotherapy with invasive fungal infections,the treatment period is short,but with mild liver and kidney function impairment,the treatment should strengthen the monitoring.

BACKGROUND: Drug therapy can improve the clinical symptoms and extend the gestational age through the maternal way to treat intrahepatic cholestasis of pregnancy (ICP), however, fetal hypoxia and meconium-stained amniotic fluid can not clearly improved. OBJECTIVE: To explore the effect of replacement of amniotic fluid that contains high concentrations of bile acids by artificial amniotic fluid through amniocentesis in the rat model of ICP. DESIGN, TIME AND SETTING: Random control animal experiments were completed in the Laboratory (first-degree laboratory) of Animal Experimental Center in Chongqing Medical University from March to September 2008. MATERIALS: Forty SD pregnant rats with 15 days of pregnancy were randomly divided into intracavitary injection of S-Adenosyl-L-methionine (SAMe) (IUS), intracavitary injection of sodium chloride (IUN), intravenous injection of SAMe (IVS) and blank control (IC) groups, with 10 rats in each group. METHODS: On day 15 of pregnancy, ICP rat models were induced by daily injection of estradiol benzoate. On day 17 of pregnancy, 1 mg SAMe and 0.3 mL sodium chloride were injected into amniotic cavity of in the IUS and IUN groups through amniocentasis. Meanwhile, 30 mg SAMe were injected though tail veins in IVS group. No treatment was performed in the IC group. MAIN OUTCOME MEASURES: The meconium-stained amniotic fluid rate and the still birth rate were calculated. Total bile acid (TBA) level in amniotic fluid was measured. In addition, the expression of hypoxia-inducible factor 1 ?(HIF-1?) mRNA in the placenta of rats were examined by immunohistochemistry and reverse transcription PCR at the days 20 of pregnancy. RESULTS: The meconium-stained amniotic fluid rate in the experimental group was lower than that of the IC group (P

Some human leucocyte antigens(HLA),espeually class Ⅱ genotypes such as HLA DQ/DR are closely linked to type 1A diabetes mellitus(T1ADM).But the genetic susceptibility of HLA DR/DQ are different between Caucasian and Asian populations.The aspartic acid at position 57 of DQ? can not confer resistance to T1ADM in Asians.DQ2 and DQ8 are the well-known susceptible genotypes in Caucasians while DQ4 and DQ9 are in Asians.An unexpected high risk of T1ADM is observed for the linkage disequilibrium between DRB1*0301/ DRB1*0401 and DQ2/DQ8 in both the populations.The DQB1*0302,DQA1*0501,DRB1*0401,*0402,*0407 are associated with T1ADM in Caucasians,but it is not the case in Asians.In contrast,DQB1*0401 shows a positive association to T1ADM in Asians,has a protective effect against T1ADM in Caucasians.Additionally DRB1*09 could be another susceptible gene in Asians.

Objective To evaluate the diagnostic value of rapid ELISA method in detecting D‐dimer combined with cardiac tro‐ponin I (cTnI) for early exclusion of acute myocardial infarction (AMI) .Methods The samples from 178 consecutive patients with chest pain and suspected AMI were tested for cTnI and D‐dimer on admission;then cTnI was also tested at 6 h after admission . AMI was definitely diagnosed by echocardiography combined with angiography .The cTnI cut‐off for diagnosing AMI was 0 .11μg/L (accounting for 10% of total CV lowest level) .Results AMI was diagnosed in 40 cases(22 .4% ) .The negative prediction value (NPV) on admission was 87 .9% for single cTnI and increased to 98 .4% at 6 h after admission .The cut‐off for single D‐dimer on admission was 200 ng/mL FEU and its NPV was 87 .9% .No false negative was observed when both cTnI and D‐dimer were nega‐tive on admission .Conclusion The combination detection of cTnI and D‐dimer has the potential to be used as an early exclusion di‐agnosis of suspected AMI in the patients with chest pain at arriving in the emergency department ,thus shorten the patient′s hospi‐tal duration and save costs .

Objective To evaluate the efficacy of D98A,a newly developed superparamagnetic oral MRI contrast agent,in the diagnosis of abdomen.Methods Plain and contrast MRI after taking 500～1000 ml D98Ain 80 patients were performed by using different field strength MR scanners,and the sequences included T 1WI,T 2WI,and fat suppressed sequences.The contrast effect,distribution,influence on demonstration of contours of abdominal organs,and side effects were studied concerning this suspension.Results It showed that this oral contrast agent had good security character,wide consideration area,low cost,good contrast effect,and high value of clinical application.The gastrointestinal tracts appeared as hypointensity on various MR sequences,and thus,made them easy to be identified.The margins of abdominal organs were easier to be located after taking the contrast,and the distinction had statistical denotation.The demonstration rate of gastrointestinal tracts was raised from 10% to 89% after using D98A.There were no side-effects in all patients. Conclusion D98A is safe and effective in facilitating the localization of the abdominal organs.It can improve the accuracy in delineating the gastrointestinal tracts and in diagnosing the lesions of abdominal organs.

OBJECTIVE:To establish the method for content determination of?-Schisandrin in Lingzhi granules.METH_ ODS:HPLC method was adopted and a C 18 column was used.The mobile phase was acetonitrile-0.1%H 3 PO 4 of water solution(67∶33).The detection wavelength was at254nm.RESULTS:The calibration curve of?-Schisandrin was linear in the range of0.0816?g～0.816?g(r=0.9999),and the average recovery was99.21%(RSD=0.79%).CONCLUSION:The method is simple and accurate,and can be used for the quality control of Lingzhi granule.