OBJECTIVE:To analyze and identify the liposoluble constituents in Isatis indigotica.METHODS:The liposoluble constituents in Isatis indigotica were extracted with petroleum benzin,and the constituents in methyl ester products were sperated,identified and determined by GC/MS.RESULTS:A total of 42 liposoluble compounds were detected,with fatty acid methyl esters making up 36.72%(saturated fatty acid methyl esters 8.67% and unsaturated fatty acid methyl esters 28.05%),and of which,35 were reported for the first time in the study of Isatis indigotica.CONCLUSION:The liposoluble compounds in Isatis indigotica serve as reference for its comprehensive benefits and development of its antioxydant applilication.

Objective:To assess the efficacy and tolerability of sibutramine as a weight reduction agent in treatment of overweight patients with type 2 diebetes.Methods: Forty overweight patients(body mass index ≥25 kg/m~(2)) with type 2 diebetes were randomized into sibutramine treatment group and control group(n=20).Both groups received a reduced-calorie diet(deficit of 1 045-2 090 kJ/d from an average daily intake) and continued their original treatment;sibutramine treatment group was also given sibutramine 15 mg once daily for 12 weeks.Before and after sibutramine treatment,body mass index(BMI),fasting blood glucose(FBG),fasting serum insulin(FINS),hemoglobin A_(1C)(HbA_(1C)),and serum lipids were measured in the patients.Homeostasis model assessment(HOMA) was applied to assess the status of insulin resistance(IR).Results: After treatment,BMI,FBG,HbA_(1C),_()TG,FINS and HOMA-IR all decreased(P

Optical positioning system has a wide range of application in many fields. This paper presents an optical positioning method which combines linear and plane cameras. 1-D image signal of linear camera is used to get 1-D coordinates of the targets, which can be processed quickly. We can use it to assist processing the 2-D image signals of plane camera under some constraints. Linear camera can reduce the amount of position searching and calculation in coordinates extracting of targets in 2D image, and it can also help identify multiple targets. The results showed that our method could reach an accuracy of 1.608 mm, which was about one thousandth of the measurable range, and the reconstruction time for 4 targets without geometric constraints is 23.87 ms, namely 41.9 fps.
Equipment Design ; Imaging, Three-Dimensional ; instrumentation ; Optical Devices

Objective To examine the inhibitory effect of IL-2-PE40 on the mouse corneal allograft rejection. Methods A mouse corneal graft model was set up by using C57BL/6 mice as donors and Balb/c mice as recipients. In the treatment group, IL-2-PE40 (0.6 μg/g body weight) was intraperitoneally injected from the day of surgery every 12 h until rejection happened. In the control group, the equal volume of PS was injected intraperitoneally at the corresponding time points. The transplanted cornea was observed under slit-lamp twice a week and the transplanted corneal opacity and neovascularization were rated according to Horis grading standards. It led to the determination of rejection response. The survival of transplanted cornea was regarded to be stopped when the rejection occurred. The operated eyes were observed historically on the 10th, 15th, 25th and 35th day after the surgery, and the peripheral blood was collected for measurement of T cell subgroups and T lymphocyte colonies. Results The survival time of cornea in the treatment and control groups was (30.2±2.9) days and (15.1±2.1) days respectively. In the control group, rejection occurred on the 15th day after the surgery, CD4~+ cells started rise right after the surgery and increased most obviously on the 15th day [(63.9±4.0)%] and decreased afterwards. CD4~+ cells in the treatment group were increased slightly [(42.6±4.0)%] on the 15th day. The number of CD4~+ cells in the treatment group was obviously less than in the control group (P<0.01). No significant changes in CD8~+ cells were observed in both groups. The number of T lymphocyte colonies in the control group was increased at the beginning and dropped then. No obvious change was found in the treatment group. The number of T lymphocyte colonies in the treatment group was significantly less than in the control group (P＜0.01). Conclusion IL-2-PE40 is a highly specific immunosuppressive agent. It can delay the development of corneal graft rejection and reduce the percentage of T-helper cells significantly. It also works to weaken the forming capacity of the peripheral T lymphocyte colonies.

Objective To evaluate the quality of the randomized controlled trials (RCTs) published in two key Chineze journals on clinical nutrition.Methods The articles published in CHINESE JOURNAL OF CLIN-ICAL NUTRlTION and PARENTERAL & ENTERAL NUTRITION from 2000 to 2008 were reviewed and the RCTs were identified according to criteria of the handbook of Cochrane Collaboration.The Jadad scale was used to evalu-ate the quality of these RCTs.Results Totally 238 RCT articles were published in these two journals in this peri-od.The Jadad score of all RCT articles was 1.65±0.82.Twenty-eight articles (11.76%) were of high quality and only 5 articles (2.10%) were identified 5 points.There were some problems in the RCTs design,conduction and analyses included unclear randomization methods,poor comparison,lack of inclusion and exclusion criteria.less blinding employment,unclear withdrawals and dropouts,and improper sample size.Conclusion The design and quality control of Chinese clinical nutrition RCTs still have some problems and require further improvement.

For the purpose of creating a hospital culture, the present paper summarizes theories on research-oriented organizations, addresses the significance of developing a research-oriented hospital, summarizes the means and methods for developing a research-oriented hospital according to the model of a research-oriented organization, and demonstrates the effects of a research-oriented hospital and promoting functions for hospital culture.

Objective To discuss the relativity between serum procalcitonin(PCT) level and renal impairment (RI) in critically ill patients. Methods A perspective study was performed in 60 critically ill patients. They were divided into 2 groups on the serum PCT level within 24 h: abnormal group (PCT ≥ 0.5 μg/L,38 cases) and normal group(PCT < 0.5 μg/L,22 cases). The serum creatinine (SCr) and blood urea nitrogen (BUN) were observed. Acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ ) of the patients were recorded. Calculated the sensitivity and specificity for prediction of RI as well as acute renal dysfunction (ARD). Results SCr [(188.91 ± 199.48) μmol/L]and BUN [(13.03 ±9.19)mmol/L]in abnormal group were significantly higher than those in normal group [(67.40 ± 15.35) μ mol/L and (6.91 ± 2.06) mmol/L](P< 0.01). The sensitivity of PCT forecast RI was 71.79%, and specificity was 52.38%. The sensitivity of PCT forecast ARD was 91.67% , and negative predictive value was 95.45%. Conclusion Serum PCT level has an important value for judgement of RI and ARD in critically ill patients.

Objective　To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods　The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results　①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion　There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.

Glutathione ; metabolism ; Humans ; Lipoxygenase ; metabolism ; Oxidation-Reduction ; Xenobiotics ; metabolism

Central post-stroke pain refers to pain resulting from a primary lesion or dysfunction of the central nervous system after a stroke.The prevalence of central post-stroke pain varies from 8％ to 46％.This article reviews the definition,epidemiology,pathophysiology,clinical features,and treatment of central post-stroke pain.