Accidents, Occupational ; Acute Disease ; Construction Industry ; Ethylene Dichlorides ; poisoning ; Humans ; Male ; Young Adult

Gene therapy of spinal cord injury (SCI) is the most promising method compared with the others, because it doesn't involve the problems of resource and higher exclusion which respectively exists in fetal nerve transplantation and peripheral nerve transplantation. There are two ways of gene therapy to be chosen: one is to transfer objective genes to the target-cells in vivo directly; the other is to transfer objective genes to one proper kind of transplantable cells firstly, then graft the highest expressing cells to the target-cells in vivo. To realize the transfer of genes to cells, two measures are used in common: physical or chemical measure such as micro infection et al and biochemical measure i. e. gene modified defective virus. Although there are some questions unresolved in this field, the clinical value of gene therapy of SCI in the future is depended.

ApolipoptoteinA1isthemaincomponentofhigh-densitylipoproteincholesterolintheplas-ma,which plays an important role in the lipid transportation and metabolism.It is closely related with cardio-vascular disease,liver disease,hereditary amyloidosis and so on.CA1 25 ,as an important biomarker,has been widely used for the diagnosis and monitoring treatment of ovarian cancer.Improved sensitivity and specificity of diagnosis are obtained when it is combined with other tumor markers,especially CA1 25 .As reported recently, it also is related with the generation,progress and prognosis of ovarian cancer.It has been promising to be a new kind of treatment.

OBJECTIVE:To establish an HPLC method for determining nicardipine hydrochloride(NCD) microspheres in cerebrospinal fluid(CSF) of rabbits by HPLC.METHODS:NCD microspheres were injected into the cistern of rabbits.Cerebrospinal fluid was sampled at different time points and NCD was separated on Agilent-Zorbax column at a flow rate of 1.0 mL?min-1 with column temperature of 30 ℃.The mobile phase consisted of methanol-water(75 :25,pH=6.0) and detection wavelength was set at 236 nm.RESULTS:The linear range of NCD was 2～128 ng?mL-1(r=0.999 8) with recovery of 98.07%.The RSD of intra-day and inter-day were both below 1.47%.The LOD was about 0.5 ng?mL-1.On 3～11 day after medication,the concentration of NCD fluctuated within(14.15?0.37) ng?mL-1.CONCLUSION:The method is simple,rapid and accurate for determination of NCD in cerebrospinal fluid of rabbits.

Objective To investigate the neuroprotection of different brain regions after transplantation of genetically modified myoblasts producing brain derived neurotrophic factor (BDNF) into cortices of neonatal rats subjected to hypoxic ischemic encephalopathy (HIE). Methods Seven day old pups were randomized into sham operated group (C), HIE+BDNF group (B) and HIE+mock transplantation group (A). A rat myoblast cell line expressing and secreting BDNF (BDNF(+)/L 6TG) was constructed. A stereotaxical intracerebroparenchymal transplantation of either BDNF(+)/L 6TG (B) or BDNF(-)/L 6TG (absence of BDNF, A) at 0.8 ?l of cell suspension (4?10 4/?l) into the left cortex of the brain was carried out shortly after HIE undergone by ligation of left common carotid artery followed by a 2.5 h inhalation of humidified 8% O 2+92% N 2 at 37℃. Changes of areas of different brain regions of cortex, hippocampus and striatum at different sections were observed 21 d after the procedure. Injury severity scores of brain tissues were also performed. Results Twenty one days after the manipulation, various degrees of atrophy were observed in the three regions of left hemispheres in group A or B versus contralateral corresponding regions or group C whereas this atrophy in group B was significantly reduced when compared to group A. Not only was the atrophy of cortex near the grafting point alleviated but some other regions of left hemispheres. A similar change pattern was seen in injury severity scores of the tissue. Conclusion Present data suggest that intracerebral transplantation of genetically modified myoblasts producing BDNF has a beneficial effect on the protection from damage of both circumscriptus brain tissues and some other areas distanced from the grafted place.

OBJECTIVE: To investigate the progress of clinical application of implant during distraction osteogenesis in the jaw bones, and to evaluate its biocompatibility.METHODS: The first author retrieved CNKI database from 1989 to 2009 in Chinese and Medline database from 1989 to 2009 in English with the key words of "jaw, distraction osteogenesis, treatment". A total of 56 articles were screened according to inclusion and exclusion criteria. Finally, 20 articles concerning treatment progress of distraction osteogenesis and clinical application of implant were included.RESULTS: Distraction osteogenesis implants contained intraoral or extraoral distraction device, which could be used for distraction of transport plate. A distraction was selected in accordance with patients' condition and requirement. Distraction osteogenesis had been an important manner for complicated tooth jaw facial deformity and jaw defect in oral dentofacial surgery and Orthodontic treatment. It could have effective osteogenesis in combination with various systemic therapy, local therapy and physical therapy. Presently, distraction osteogenesis implants were metal materials. Metal implants have great advantages in preventing bacterium growth, keeping implant fixation and distraction effects, and the price was high. However, the cost of Ni-Ti memory alloy wire was lower compared with common retractors. The fixing device and alloy wire closely adhered to the bone could be completely implanted into tissues, with the advantages of anti-infection and completely closing the exterior and interior wound. CONCLUSION: Distraction osteogenesis is a new method for treating dental and craniofacial hypodevelopment straightening of teeth and for repairing jaw bone defect deformation. Biocompatibility of metal implants was good such as internal small Ni-Ti memory alloy wire. The biocompatibility of other types of implants should be improved.

Applying H-index combined with other evaluation index of qualitative and quantitative evaluation for seientifie research ability, levels of researchers as well as academie journals, is becoming inereasingly concerned and will gradually be applied. The paper discusses the connotation, extension, feasibility, limitations on H-index and further analyzes the applicability of using H-index to evaluate the quality of academic journals.

Bioterrorism ; classification ; psychology ; trends ; Communicable Diseases ; transmission ; Disease Transmission, Infectious ; prevention & control ; Humans

Objective To investigate the clinical value of serum procalcitonin(PCT),C-reactive protein(hs-CRP),white blood cell count(WBC)and neutrophil percentage(NEU％)in the early diagnosis of sepsis caused by different pathogenic bacteria infection to provide a risk assessment for sepsis patients.Methods The clinical data of 117 patients with sepsis caused by bloodstream infection in the intensive care unit(ICU)of our hospital from Oct.2013 to Oct.2015 were retrospectively analyzed.All patients were performed blood culture before antibiotics use and divided into the G+ bacteria group and G-bacteria group according to the results of blood culture.PCT,hs-CRP,WBC and NEU％ were detected in all cases.The differences in the detection results between the two groups were analyzed and the correlation between PCT with APACHE-Ⅱ and SOFA scores was evaluated.Results Among 117 cases of sepsis,78 cases were in the G+ bacteria group and 39 cases were in the G-bacteria group;compared with the G+ bacteria group,PCT in the G-bacteria group had obvious change and the difference was statistically significant(P＜0.05),while the detection results of hs-CRP,WBC and NEU in the G-group had little change(P>0.05);the serum PCT level was positively correlated with the SOFA score in the G-bacteria group(r=0.536,P＜0.05),but had no correlation with the APACHE-Ⅱ score(P>0.05);serum PCT level had no correlation with the APACHE-Ⅱ score and SOFA score in the G+ bacteria group(P>0.05).Conclusion PCT has some value in the early differential diagnosis and prognosis of sepsis caused by different pathogens,and it is more sensitive to G-bacteria.

Chronic Disease ; Endoscopy ; Humans ; Paranasal Sinuses ; surgery ; Sinusitis ; surgery