Objective:To explore the effects of exercise on the spleen T lymphocyte proliferation and T lymphocyte subsets of SD rats.Methods:24 SD rats were divided into 3 groups randomly:control group,30 min exercise group,60 min exercise group;MTT and flow cytometry ( FCM ) were utilized to examine the spleen T lymphocyte proliferative capability and T lymphocyte subsets, respectively.Results:Compared with the control group,the T lymphocyte proliferation,levels of CD4+and CD4+/CD8+T lymphocytes in 30 min exercise group rats were significantly increased(P<0.05),levels of CD8+T lymphocytes in 30 min exercise group rats had no statistically significant change;the T lymphocyte proliferation,levels of CD4+,CD8+and CD4+/CD8+T lymphocytes in 60 min exercise group rats had no statistically significant changes.Conclusion: Suitable loaded exercise could enhance the cellular immune function, this is probably related with the mechanisms of improving the T lymphocyte proliferative capability and regulating the CD4+/CD8+T lymphocyte subsets.

BACKGROUND: Based on excellent hydration ability, the materials for repairing bone defects could be fabricated by three-dimensional printing from amorphous calcium phosphate simply with pure water as adhesive solution; and more importantly, the printed products could be directly used in clinical medicine without high temperature sintering, so amorphous calcium phosphate fits well with technical features of three-dimensional printing. OBJECTIVE: To prepare bone repair materials of amorphous calcium phosphate with mechanical property and printing accuracy to meet practical application requirements by three-dimensional printing. METHODS: Amorphous calcium phosphate used as prototyping powder was prepared by coprecipitation method, and then the viscosity and surface tension of the deionized water as adhesive solution were adjusted by thickening agent and leveling agent, respectively. Afterwards, the three-dimensional printing productions for repairing bone defects were fabricated, and the effects of the viscosity and surface tension of adhesive solution on the forming of droplet, liquid-solid interaction and the mechanical property as well as printing accuracy of three-dimensional printing productions were investigated. RESULTS AND CONCLUSION: By investigating the forming of droplet and liquid-solid interaction, the optimal physicochemical parameters of the adhesive solution were obtained. The viscosity and surface tension of the optimal adhesive solution were 8.0 × 10-3 Pa•s and 40.0 × 10-3 N/m separately, and at this point, not only droplet could form stably and controllably (Z=5.06), but also it smoothly struck the powder layer during spraying (K=14.29), and then it infiltrated into the powder layer uniformly and spread in time (We=36.86). The corresponding three-dimensional printing production has good mechanical properties (compressive strength is 30.4 MPa), high printing accuracy (forming error is 0.9 mm), and a large number of pores indicating good bone conductivity, which partially meets clinical demands of repairing bone defects.

Objective To compare the clinical effectiveness of incision-thread-drawing procedure and incision and drainage procedure in the treatment of perianal abscess.Methods Clinical data collected from 110 patients with perianal abscess were retrospectively analyzed.Forty-six cases were treated with incision-thread-drawing procedure(therapy group) and 64 with incision and drainage procedure(control group).Results Recurrence rate and incidence of anal fistula were 4%(2/46) and 4%(2/46) in therapy group,respectively,significantly lower than those in control group(19% and 23%,P

The fibroblasts have long been considered as structural elements and playing a main role in wound healing. Recent studies indicated that fibroblasts are heterogenic cells and function as resident sentinel cells in inflammatory reaction. When activated by substances released during tissue injury or derived from infectious microorganisms or by proinflammatory cytokines, tissuespecific fibroblasts can transiently produce certain chemokines and initiate a cascade of inflammatory reaction. In addition fibroblasts can produce prostanoids that participate in inflammatory and immune responses. In this review, the new role of fibroblasts in regulation of inflammation and immune reactions and possible molecular mechanism will be discuss.

Objective To investigate the clinical feature, diagnosis and treatment for primary splenic space occupying lesions. Methods 13 cases with primary splenic space occupying lesions were retrospectively analyzed. Results Splenectomy and pathological diagnosis was performed in all cases,in which 10 cases were benign and 3 ca-ses were malignant. All discharged after cure. Conclusion Positioning diagnosis of primary splenic space occupying lesions depend on imaging examination(USG and CT) ,determinant diagnosis need pathological examination. This dis-ease should be accepted early surgery, splenectomy is basic operation.

Animals ; Chick Embryo ; physiology ; Electrocardiography ; methods ; Heart ; embryology ; physiology ; Heart Rate ; physiology

Objective To explore the possible role of vitamin D in the pathogenesis of IgA nephropathy (IgAN). Me-thods After the IgAN model was successfully induced at 12 weeks, the BALB/C mice were randomly divided into IgAN group (n=15) and IgAN+VitD group (n=15). The nephrosis mice were administrated with 100 μl/d propylene glycol or propyl-ene glycol+1,25(OH)2D, 3 ng/(100g?d), for 6 weeks. The control group was setted (n=15). The level of 24 hour urine protein was determined at week 0, 12 and 18. At week 18, the levels of serum 25(OH)D, ifbroblast growth factor 23 (FGF23) and galactose-deifcient IgA1 (Gd-IgA1) were detected. The mRNA and protein expressions of interleukin-21 (IL-21) in Peyer’s patches (PPs) were detected by lfuorescent quantitative reverse transcription-polymerase chain reaction and western blot respectively. The protein expression of Bcl-6 was detected by western blot. The percentages of Tfh cells/T lymphocytes, B220+IgM+/B lympho-cytes, B220+IgA+/B lymphocytes, B220-IgA+/B lymphocytes in PPs were determined by lfow cytometry. Results Compared with control group, the levels of 24 hour urine protein, FGF23 and Gd-IgA1 were increased, serum 25(OH)D was decreased, the mRNA and protein expressions of IL-21 and the protein level of Bcl-6 were increased, the percentages of Tfh cells/T lym-phocytes, B220+IgM+/B lymphocytes, B220+IgA+/B lymphocytes, B220-IgA+/B lymphocytes were elevated in IgAN group (P<0.05). These indicators were improved in IgAN+VitD group. Compared with the IgAN group, the differences were statisti-cally signiifcant (P<0.05), however compared with control group, some indicators showed no signiifcant differences (P>0.05). Conclusions 1,25(OH)2D may protect the microenvironment of PPs in IgAN through inhibiting the differentiation of Tfh cells and B cells and the generation of Gd-IgA1.

Objective To detect the changes in protein profile of rats'lung inhaled radon using two- dimensional electrophoresis(2-DE)and MS.Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100,200 and 400 working level months(WLM).The total proteins of rats'lung were extracted and isolated by 2-DE.The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS.Resuits Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots,15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS.Conclusions The 2-DE image of radon groups'lung has changed,and the lung damage effect of radon is related to some proteins.

0.05).Combination of NGF with bFGFs(10 U/ml NGF+10 ?g/L bFGF or 5 U/ml NGF+5 ?g/L bFGF) not only promoted the proliferation of HDPCs(P

Objective To investigate the prevalence of tumor necrosis factor-?(TNF-?)promoter polymorphisms(at position-308 or-238)in patients with nonalcoholic fatty liver disease(NAFLD),and to explore the relationship between TNF-? promoter polymorphisms and obesity and the insulin resis tance(IR)in those patients.Methods Metabolic variables were measured in 117 patients with NAFLD, among them 60 were obesity and 57 were non-obesity.Genotype of TNF-? promoter were determined by PCR-RFLP.Plasma TNF-? level was measured by ELISA.Indexes of IR and insulin secretion were determined using the homeostasis model assessment method.One hundred and twenty normal subjects who were matched for age and sex served as control.Results The G→A mutation of the-238 TNF-? polymorphism was significantly higher in patients with NAFLD versus controls(29.9% vs.15.8%, P