Glucocorticoid(GC) /glucocorticoid receptor(GR) inhibits cell proliferation and protects cells from apoptosis induced by serum deprivation,hypoxia,irradiation or chemotherapy drugs in many cell types,thereby GC/GR can maintain cell number homeostasis,which is an important aspect of homeostasis regulation of GC/GR in higher organisms.This article reviews antiproliferative and antiapoptotic effects of GC/GR on cell and their molecular mechanism,mainly focusing on the molecular events and target genes downstream of GC/GR.

Objective To understand the current status of intestinal helminth infections in Dafeng City. Methods The resi?dents in 5 villages of Dafeng City were investigated and their stool samples were detected for the eggs of helminth with Kato?Katz technique and Enterobius vermicularis was detected by the cellophane anal swab method. Results The total infection rates of intestinal helminth were 5.77% 5.51% 4.60% 4.18% 3.41% and 1.38% from 2005 to 2010 respectively. The trend of total infection rates declined year by year. The infection rates in the 20?30 age?group and 60?80 age?group were higher than those in other age?groups. The infection rates of the male and female were 5.63 359/6 375 and 2.42 144/5 949 re?spectively and there was a significant difference χ2=74.81 P=0.00 . The infection rate 11.70% in the northern areas of Dafeng City was higher than that in other places and the trend of the infection rates decreased from the eastern and northern to the western and southern. The infection rate of E. vermicularis was 1.75%in children in 2010. Conclusions The infection rate of intestinal helminth is low but E. vermicularis infection is relatively general in the children in Dafeng City. Therefore the pre?vention and treatment still need to be strengthened.

Humans ; Multiple Myeloma ; Neoplasm Recurrence, Local

OBJECTIVE:To determine the content of hydroxyproline in liver tissue in rats with hepatic fibrosis.METHO_ DS:RP-HPLC was used with precolumn derivation by2,4-dinitrochlorobenzene(DNCB)in the Borax buffer solution for lh in boiling water.Acetonitrile-acetate buffer linear gradient elution was used.Detecting wavelength was360nm.RESULTS:Derivation of hydroxyproline could be separated from other amino acids and accurately determined.CONCLUSION:The method is rapid,accurate,and suitable for detection of biopsy samples obtained by liver puncture.

Objective Vessel overload index (VOI)was studied to monitor patients with hypertensive cerebral hemorrhage.Methods 144 cases with hypertensive cerebral hemorrhage were divided into two groups at random.One group was treated with mannitol plus urapidil hydrochlorid and the other with mannitol alone as controls.VOI were observed before and 2～4 hours after treatment and the changes of VOI values were analyzed with t-test.Results There was no significant difference in VOI values between groups before treatment (P＞0.05),but after treatmen (P＜0.05),30 day mortality was 18.18% in controls and 8.7% in observed groups.Conclusion VOI can be used to evaluate vessel load in hypertensive patients with cerebral hemorrhage and to indicate hypotensive therapy properly.

Objective To get a better understanding of the characteristic MRI appearances of cerebral MS lesions.Methods 61 patients with clinically diagnosed multiple sclerosis were examined with SE sequences,using 0.5T super-conducting scanner.The MRI features of MS were analyzed.Results All of the patients in 61 cases were found MS lesions in MRI.The lesions in cerebral,brainstem,and cerebellum were clearly visualized,but in optic nerves were not so good.The MS foci appeared low or middle signal intensity in T 1WI and high signal intensity in T 2WI.It was playing an important role in the diagnosis of the MS that the paraventricular lesions were perpendicular to the lateral ventricles and there were MRI abnormal signals in corpus callosum with shrinking. Conclusion MRI can show the characteristic appearances of MS lesions,demonstrate many more lesions that not visible on CT scan,provide informations about the number,size,location and extent of the lesions therefore MRI is the first method of choice in the diagnosis of MS.

Purpose:To investigate whether transforming growth factor ?1 (TGF ?1) pathway is involved in the mechanism of dexamethasone (Dex) mediated proliferation inhibition in ovarian cancer cell line HO 8910.Methods:To analyse cell proliferation and cell cycle distribution by cell counts and flow cytometric analysis, respectively ; to determine the expression levels of TGF ?1 and its two receptors, T?R Ⅰ and T?R Ⅱ , by quantative RT PCR, ELISA and(or) immunocytochemistry methods.Results:Dex induced a G 0 /G 1 cell cycle arrest in HO 8910 cells, and it up regulated T?R Ⅱ expression in a concentration dependent manner.The level of T?R Ⅱ mRNA was the highest after treatment with Dex for 8 hours, with 1.4 fold more than that of control at concentration of 10 -7 mol/L ( P

AIM: To study the protection effect of baicalin on isoproterenol (ISO) induced myocardial ischemia in rats and its mechanisms. METHODS: Forty-five Sprague-Dawley rats were randomly divided into 3 groups. The rats in control group (n=15) received no treatment. The rats in ISO group (n=15) received two times of subcutaneous injection of ISO (20 mg?kg~ -1), which was separated by a 24 hours-interval. The rats in baicalin group (n=15) received ISO as described above and received baicalin 50 mg?kg~ -1 ip. simultaneously. Two days later the hemodynamic measurement was performed and the serum levels of LDH, CK and TNF-? were detected. The expression of p38mapk in myocardium was detected by immunohistochemical technique. RESULTS: The heart function of the group treated with baicalin was improved significantly compared with that of control group. The levels of LDH, CK and TNF-? in baicalin group decreased significantly compared with those in control group and the expression of p38mapk in myocardium also decreased markedly. CONCLUSION: Baicalin has protection effect on isoproterenol induced myocardial ischemia in rats, and the effect maybe due to the decrease of TNF-? induced by reducing the p38mapk expression.

Objective To observe the expression of CD_(25),CD_(25) mRNA,interleukin-1?(IL-1?) in peripheral blood of newborns with hypoxic-ischemic encephalopathy(HIE) and its clinical significance.Methods The peripheral blood mononuclear cells(PBMC) of newborns with HIE and normal controls were isolated by routine Ficoll-Hypaque,and the level of CD_(25) induced and non-induced by phytohernagglutinin(PHA) were detected by biotin-streptavidin(BSA),CD_(25) mRNA was detected by real-time PCR and IL-1? in serum was detected by enzyme-linked immunosorbent assay on the first,third and seventh day after birth.The final expressive level of CD_(25) mRNA was based on rate of CD_(25) cDNA and gluceraldehyde phosphase dehydrogenase(GAPDH).Results On the first day,the positive rates of CD_(25) induced and non-induced by PHA and IL-1? were(3.6?1.1)%,(20.9?4.8)%,(17.7?8.2) ng/L,respectively.There were significant differences in HIE group and normal controls(P0.05).Conclusions There is obvious inflammatory(rea)ction in babies with HIE.The main character of PBMC of newborns is all infantile and undifferentiated,which surface sign is infantile,and lower level of CD_(25) is expressed.However,the level of CD_(25) mRNA can be expressed consistently during the course of disease.The immunity and immune regulative response in newborns is gradually built up during the growth and can participate in recondition of brain injury caused by HIE.

Posterior scleritis,an inflammatory disease of the posterior sclera,is most commonly seen in middle-aged women and often associated with systemic immunological diseases.It presents with a variety of nonspecific signs and symptoms,and its clinical feature may be confused with ocular tumors,orbital inflammation,and retinochoroidopathy.Therefore,some auxiliary examinations are necessary for the diagnosis of posterior scleritis.With early proper diagnosis and treatment,severe complications of posterior scleritis can be avoided or lessened.In the paper,some advancement in the study of etiology,incidence,signs,symptoms,auxiliary examination,differential diagnosis and treatment of posterior scleritis were reviewed.